SAMPLING OF ODOUROUS AIR FOR OLFACTOMETRIC MEASUREMENT J. HARTUNG Institute for Animal Hygiene of the Hannover School of Veterinary Medicine, Blinteweg 17 p , 3000 Hannover 71, FRG Summary Both static and dynamic sampling procedures are used for olfactometric measurements. Care must be taken inorder to obtain a representative sample and to minimize sample losses due to condensation, adsorption and permeation, when using static sampling methods, particularly. Teflon or Tedlar bags and inert tubing materials help to diminish adsorption and desorption problems. Condensation can be avoided by heating the sampling tubes or by prediluting the sample with pure, odour-free air. Within the EEC guide lines exist for odour measurement in The Netherlands, France, Germany and the United Kingdom. The usefulness of dynamic sampling is agreed on. The opinions differ as far as static sampling is concerned. It seems that both sam­ pling methods can be applied successfully for olfactomet-ric measurements. However, it is necessary to define the details of the procedures aiming at a standardization of sampling which might be the first step for a harmoni­ zation of olfactometric measurements in the laboratories of the different countries. 1 . INTRODUCTION The method of measuring odour sensorily in general can be devided into the following basic steps (1): - sample collection - sample dilution and presentation - indication of response - interpretation of response Due to the fact that many different testing procedures exist in the different laboratories, results can only be com­ pared when knowing exactly - the conditions and procedures for sampling of the air to be i nvesti gated, - the design and function of the olfactonnetric apparatus, and - the physiological and physical status of the panel. The olfactometric apparatus and the panel are in close connection with each other as shown in Table I whereas the sam­ pling procedure is more or less apart from the apparatus and the panel and affects the olfactometric inlet, only. However, sample collection is the first step and can influence the re­ sults considerably; thus, valid sampling is the base for valid

RECOMMENDATIONS FOR SAMPLING AS DESCRIBED BY GUIDELINES Four guidelines exist in the states of the EEC concerning odour: - The Netherlands: Geurnormering - Odour standard (1983) - France: Norme experimentale X43-101 - Experimental standard, 1st draft, (1982) - Germany: VDI-Guideline 3881, part 2, draft (1984) - U.K.: Odour control - a concise guide (Warren Springs, 1980) Only the guideline of Warren Springs, U.K., and the VDI-Guide-line contain detailed descriptions of the sampling procedure. Concerning both dynamic and static sampling there is an agreement to avoid adsorption and condensation By insulating and heating the inlet tubes, and by prediluting the sample with dry odour-free air when bag or vessel sampling is used. Desorp­ tion problems can be minimized by preflushing. Inert materials have to be used, only. The storage time of the sample in bags should be as short as possible. If required dust filters can be provided to prevent a contamination of the olfactometer by dust. The most detailed description of sampling is given in the German VDI-Guideline 3881. The recommendations do not prefer a certain technique but accept different techniques if they meet the requirements to prevent condensation, adsorption, influ­ ences of the dust, and desorption of odorous compounds from the sampling system. The guideline points out that these im­ portant problems should, if necessary, be settled in prelimi­ nary experiments. The guideline includes the description of a dilution equipment to avoid condensation. Figure 4 shows the forms of static sampling given in the Guideline. The Warren Springs, U.K. guide gives a full description of the recommended sampling procedure and apparatus, as shown in Figure 5. Tedlar bags of a capacity of 40-50 1 are used.The bags are introduced in a 60 1 carboy. All tubes except for the underpressure tube which is made of metal consist of PTFE in­ cluding the valves. A small battery-operated pump is used to evacuate the container, which allows the initially collapsed Tedlar-bag to inflate (14). 5. FINAL REMARKS Both dynamic and static sampling procedures are suitable for taking samples for olfactometric measurements (15), (16). If the olfactometer and the panel are available close to the source dynamic sampling may be preferred. The equipment for preventing condensation in the sampling pipe and contamination of the sampling pipe and the olfactometer by dust should be provided. Static sampling may be used at sources of odour that are not readily accessible or where the odour concentrations are changing quickly or because of expenses. When using static sampling the most important requirements are to avoid losses of sample-born compounds by adsorption and condensation and the contamination of the sample by impurities desorbing from the sampling and storing divice. Interaction of the compounds in the sample during storage can be minimized by keeping the time


of storage as short as possible, only; 24 h should not be ex­ ceeded. Table III comprises the most important criteria for valid static and dynamic sampling. It seems that both the guide of Warren Springs, U.K. and the VDI-Guideline might be a useful base to describe commonly accepted sampling procedures aiming at a standardization of sampling which might be a first step for a harmonization of olfactometric measurements in the different laboratories and countri es. REFERENCES (1) BULLEY, N.R. and D. PHILLIPS (1980). Sensory evaluation of agricul­ tural odours: A critical review. Can. Agric. Eng. 22, 107 - 112. (2) HENRY, J.G. and R. GEHR (1980). Odour control: An operator's guide. Journal WPCF 52, 2523 - 2537. (3) ROOS, C., J.A. DON and J. SCHAEFER (1984). Characterization of odour-polluted air. In: Proc.Int.Symp., Soc. Beige de Filtr. (eds.), 25-27 April 1984, Louvain-La-Neuve, Belgium, pp. 3 - 22. (4) BAKER, A.R. and R.C. DOERR (1959). Methods of sampling and storage of air containing vapors and gases. Int.J.Air Poll. 2, 142 - 158. (5) SCHUETTE, F.J. (1967). Plastic bags for collection of gas samples. Atmosph.Environm. 1, 515 - 519. (6) SCHODDER, F. (1977T. Messen von Geruchsstoffkonzentrationen, Erfassen von Geruch. Grundl. Landtechnik 27, 73 - 82. (7) CORMACK, D., T.A. DORLING and B.W7J. LYNCH (1974). Comparison of tech­ niques for organoleptic odour-intensity assessment. Chem.Ind. (Lon­ don) no. 2, 857 - 861. (8) SCHUETZLE, D., T.J. PRATER and S. RUDDELL (1975). Sampling and anal­ ysis of emissions from stationary sources. I. Odour and total hydro­ carbons. APCA Journal 25, 925 - 932. (9) WAUTERS, E., E. WALRAVENS, E. MUYLLE and G. VERDUYN (1983). An evalu­ ation of a fast sampling procedure for the trace analysis of volatile organic compounds in ambient air. Environm.Monitor.Assessm. 3, 151-160. (10) LACHENMAYER, U. and H. KOHLER (1984). Untersuchungen zur Neuentwick-lung eines Olfaktometers. Staub - Reinhalt. Luft 44, 359 - 362. (11) BERNARD, F. (1984). Simplified methods of odour measurement: Indus­ trial application and interest for administrative control. Proc. Int. Symp., Soc. Beige de Filtr. (eds.), 25 - 27 April 1984, Louvain-La-Neuve, Belgium, pp. 139 - 150. (12) GILLARD, F. (1984). Measurement of odours by dynamic olfactometry. Application to the steel and carbonization industries. Proc.Int.Symp., Soc. Beige de Filtr. (eds.), 25 - 27 April 1984, Louvain-La-Neuve, Belgium, pp. 53 - 86. (13) MANNEBECK, H. (1975). Tragbare Olfaktometer. VDI-Bericht 226, 103-105. (14) BEDBOROUGH, D.R. (1980). Sensory measurement of odours. In: Odour Control - a concise guide, F.H.H. Valentin and A.A. North (eds.), Warren Springs Laboratories, Stevenage, Hertfordshire, U.K., pp. 17-30. (15) THIELE, V. (1984). Olfaktometrie an einer Emissionsquelle - Ergebnis-se des VDI-Ringvergleichs. Staub - Reinhalt. Luft 44, 342 - 351. (16) DUFFEE, R.A., J.P. WAHL, W. MARRONE and J.S. NADERT1973). Defining and measuring objectionable odors. Internat. Pollution Eng. Congress, Philadelphia, paper no 25a, pp. 192 - 201.


2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
John A. Griffioen ◽  
Devorah M. Stowe ◽  
Macy Trosclair ◽  
Larry J. Minter ◽  
Chelsey Vanetten ◽  
...  

Biochemical testing is an important clinical tool in evaluating the physiology of reptiles and amphibians. Suitable point of care analyzers can allow for rapid delivery of results, but small patient size can inhibit sufficient sample collection. This study evaluated the utility of sample dilution with sterile distilled water as a means of biochemical evaluation when sample volume is limited. Blood was collected from 12 eastern box turtles (Terrapene carolina carolina) and 12 marine toads (Rhinella marinus) and analyzed via i-STAT CHEM8+ cartridges. Two undiluted samples and two samples diluted 1 : 1 with sterile water were evaluated immediately following collection for each animal in the study. Analytes reported in the diluted samples were limited to glucose, ionized calcium, and total carbon dioxide. The expected dilution ratio value of diluted to undiluted samples was 0.5, of which glucose in both turtles and toads was nearest. Dilution ratio values for ionized calcium, however, were higher than expected in both turtles and toads. Sample dilution is not recommended for most analytes included on the CHEM8+ cartridge due to values occurring outside the limits of detection for the analyzer. Glucose and ionized calcium values obtained on diluted samples should be interpreted with caution but may provide clinical utility in reptile and amphibian patients where sample volume is limited.


Radiocarbon ◽  
2017 ◽  
Vol 59 (3) ◽  
pp. 713-726 ◽  
Author(s):  
J-P Dumoulin ◽  
C Comby-Zerbino ◽  
E Delqué-Količ ◽  
C Moreau ◽  
I Caffy ◽  
...  

AbstractThe main objective of this report is to present the dating process routinely applied to different types of samples at the Laboratoire de Mesure du Carbone 14 (LMC14). All the results and protocols refer to our procedures over the last 5 years. A description of the sorting and chemical pretreatments of the samples as well as the extraction and graphitization of CO2 are reported. Our last study concerning the degradation of the blank level according to the storage time of the targets between graphitization and accelerator mass spectrometry (AMS) measurement is also presented. This article also provides information on how to submit a valid laboratory sample. We give details relating to sampling procedures on site as well as contamination issues relative to the 14C dating methodology.


English Today ◽  
2001 ◽  
Vol 17 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Lee-Wong Song Mei

An examination of the culture, identity and function of English in Singapore.The widespread use of English in Singapore has placed Singapore in the Outer Circle, along with India, Pakistan, Sri Lanka, Ghana, Nigeria, the Philippines and others, in contrast with Australia, the United Kingdom, the United States and others who belong to the Inner Circle (Kachru,1991). Placing countries in different circles – inner vs outer or expanding, based on the concept of nativization – has generated questions of democracy in linguistic ideology and related issues of norms and standards. These are discussed.


2018 ◽  
Author(s):  
Brian McMillan ◽  
Robert Eastham ◽  
Benjamin Brown ◽  
Richard Fitton ◽  
David Dickinson

UNSTRUCTURED This paper briefly outlines the history of the medical record and the factors contributing to the adoption of computerized records in primary care in the United Kingdom. It discusses how both paper-based and electronic health records have traditionally been used in the past and goes on to examine how enabling patients to access their own primary care record online is changing the form and function of the patient record. In addition, it looks at the evidence for the benefits of Web-based access and discusses some of the challenges faced in this transition. Finally, some suggestions are made regarding the future of the patient record and research questions that need to be addressed to help deepen our understanding of how they can be used more beneficially by both patients and clinicians.


2021 ◽  
Vol 156 (Supplement_1) ◽  
pp. S31-S31
Author(s):  
R Alqabbani ◽  
D Stickle

Abstract Introduction/Objective Our cardiac intensive care unit (CICU) asked the laboratory to provide plasma hemoglobin testing to monitor effects of ventricular-assist devices (VAD). Roche hemolysis index (HI) has been reported to be suitable for this purpose. Use of HI as a reportable test, however, must be validated in-house as a laboratory- developed test (LDT), according to CLIA regulations. We describe results of our evaluation, and caveats for intended use. Methods/Case Report Concentrated hemolysate was produced by osmotic lysis of packed red blood cells in water. Hemolysis in plasma was by dilution of concentrated hemolysate. Verification of HI as a measure of hemoglobin (mg/dL) used the Sigma-Aldrich spectrophotometric hemoglobin assay as a gold standard. Linearity, linear range, sensitivity, were investigated by dilution measurements; interferences by admixture experiments; reproducibility (precision, intra- and inter-assay) in each case by replicate measurements (n = 20). Results (if a Case Study enter NA) HI was confirmed to correspond to hemoglobin in mg/dL. Linearity was between 2-1000 mg/dL (r2 >0.99). Intra-assay precisions were <=2.5% (hemoglobin = 74,148 mg/dL); inter-assay precisions were <=4.9% (hemoglobin = 69,139 mg/dL). HI variability was ±2 at very low values (0-10 mg/dL). There were no interferences observed among common therapeutic drugs. Hyperlipemia (evaluated up to lipemic index (LI) = 225) and hyperbilirubinemia (evaluated up to icteric index (II) = 7) demonstrated no significant interference. Conclusion Analytically, HI exhibited acceptable performance for reporting of plasma hemoglobin. Deployment would require establishment of quality control (QC) and proficiency testing procedures for HI. Clinically, a caveat for use is that HI cannot distinguish between hemolysis in circulation vs. that due to sample collection. For the CICU, HI >10 mg/dL is characteristic of more than 20% of samples drawn in general, irrespective of use of VAD. Temporal patterns of HI must therefore be carefully evaluated in parallel with haptoglobin measurements to assist in VAD management.


10.2196/18218 ◽  
2020 ◽  
Vol 22 (11) ◽  
pp. e18218
Author(s):  
Helen Atherton ◽  
Anne-Marie Boylan ◽  
Abi Eccles ◽  
Joanna Fleming ◽  
Clare R Goyder ◽  
...  

Background Increasingly, consultations in health care settings are conducted remotely using a range of communication technologies. Email allows for 2-way text-based communication, occurring asynchronously. Studies have explored the content and nature of email consultations to understand the use, structure, and function of email consultations. Most previous content analyses of email consultations in primary care settings have been conducted in North America, and these have shown that concerns and assumptions about how email consultations work have not been realized. There has not been a UK-based content analysis of email consultations. Objective This study aims to explore and delineate the content of consultations conducted via email in English general practice by conducting a content analysis of email consultations between general practitioners (GPs) and patients. Methods We conducted a content analysis of anonymized email consultations between GPs and patients in 2 general practices in the United Kingdom. We examined the descriptive elements of the correspondence to ascertain when the emails were sent, the number of emails in an email consultation, and the nature of the content. We used a normative approach to analyze the content of the email consultations to explore the use and function of email consultation. Results We obtained 100 email consultations from 85 patients, which totaled 262 individual emails. Most email users were older than 40 years, and over half of the users were male. The email consultations were mostly short and completed in a few days. Emails were mostly sent and received during the day. The emails were mostly clinical in content rather than administrative and covered a wide range of clinical presentations. There were 3 key themes to the use and function of the email consultations: the role of the GP and email consultation, the transactional nature of an email consultation, and the operationalization of an email consultation. Conclusions Most cases where emails are used to have a consultation with a patient in general practice have a shorter consultation, are clinical in nature, and are resolved quickly. GPs approach email consultations using key elements similar to that of the face-to-face consultation; however, using email consultations has the potential to alter the role of the GP, leading them to engage in more administrative tasks than usual. Email consultations were not a replacement for face-to-face consultations.


2007 ◽  
Vol 41 (3) ◽  
Author(s):  
Ben De Klerk

Basic-theoretical foundations of the blessing in the worship service and ensuing guidelines for the liturgy The Biblical foundations of the blessing in the church service and the meaning and function of the salutatory and sending- away blessings are the focus of this article. A short extract from history follows, and based on findings from Scripture and his- tory, theoretical perspectives for practice and liturgical guide- lines are indicated. Some of the most important conclusions drawn are the following: The congregation of Jesus Christ should receive the blessing of the Lord as indispensable gift in its assemblage. Without this blessing communion with God and each other, trust in God and fulfilment of each believer’s voca- tion in the world are impossible. The salutatory blessing pours out onto the congregation all the blessings that enable mem- bers to participate to advantage in the church service. The congregation is blessed and sent back into the world by God himself. Without the sending-away blessing the congregation cannot fulfil its vocation in the world, especially to be witnesses for Christ. If an ordained minister of the gospel is present in the gathering of the congregation he should pronounce the bles- sing, which comes directly from God. It is vital that the congre- gation appropriate the blessing in faith by means of an overt act, namely by saying “amen” to that.


2021 ◽  
Vol 14 (12) ◽  
pp. 733-741
Author(s):  
Frith Cull ◽  
Holli Coleman

Bell's palsy is the term given to an idiopathic lower motor neurone facial nerve paresis or paralysis. It is of rapid onset, almost always unilateral, and may be associated with facial or retro-auricular pain or otalgia. It is the most common diagnosis associated with facial nerve palsy; a GP will see a case approximately every 2 years in practice in the United Kingdom. Early diagnosis and steroid treatment increase the likelihood of full recovery, whereas ocular complications can be prevented by lubricants and lid taping. Over 70% of patients recover within a year. Options to improve facial appearance and function, in those who do not experience a complete recovery, include surgery.


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