Gene Expression and Physiological Responses in Mexican Maize Landraces under Drought Stress and Recovery Irrigation

2011 ◽  
pp. 74-103
Author(s):  
Corina Hayano-Kanashiro ◽  
Carlos Calderón-Vázquez ◽  
Enrique Ibarra-Laclette ◽  
Luis Herrera-Estrella ◽  
June Simpson
Keyword(s):  
Gene Expression ◽  
Drought Stress ◽  
Physiological Responses ◽  
Maize Landraces ◽  
Mexican Maize

scholarly journals Analysis of Gene Expression and Physiological Responses in Three Mexican Maize Landraces under Drought Stress and Recovery Irrigation

PLoS ONE ◽  
2009 ◽  
Vol 4 (10) ◽  
pp. e7531 ◽  
Author(s):  
Corina Hayano-Kanashiro ◽  
Carlos Calderón-Vázquez ◽  
Enrique Ibarra-Laclette ◽  
Luis Herrera-Estrella ◽  
June Simpson
Keyword(s):  
Gene Expression ◽  
Drought Stress ◽  
Physiological Responses ◽  
Maize Landraces ◽  
Mexican Maize

scholarly journals Morphological, physiological and molecular responses of Indonesian cassava to drought stress

2020 ◽  
pp. 1723-1727
Author(s):  
Sholeh Avivi ◽  
Bella Rhea Lavifa Sanjaya ◽  
Shinjiro Ogita ◽  
Sri Hartatik ◽  
Sigit Soeparjono
Keyword(s):  
Gene Expression ◽  
Hydrogen Peroxide ◽  
Drought Stress ◽  
Physiological Responses ◽  
Plant Cells ◽  
Gene Expressions ◽  
Molecular Responses ◽  
Molecular Features ◽  
Drought Tolerant ◽  
Cassava Varieties

In Indonesia, investigations of drought-tolerant cassava by characterizing the morphological, physiological, and molecular responses have not been carried out. This research was aimed to characterize the morphological, physiological, and molecular features of 10 Indonesian cassava varieties (Adira 1, Malang 1, Cimanggu, Kaspro, Ketan, Litbang UK-2, Malang 4, Malang 6, UJ-3, and UJ-5) under drought stress. 30 days after planting, drought stress was applied by stopping irrigation of plants for 15 days. The plant height, root system, and wilting were measured as morphological responses of cassava. For physiological responses, the percentage of opening stomata, chlorophyll, and hydrogen peroxide (H2O2) content were also investigated. Gene expression of MeGBF3 and MeMSD was evaluated to analyze the response of candidate drought-tolerant genotypes. The studied parameters revealed that drought stress inhibits the growth of cassava. Some genotypes could not survive because the excessive content of H2O2 would be toxic to plant cells and disturb the plant growth. The up-regulated gene expressions of MeGBF3 and MeMSD has correlations with morphological and physiological responses of cassava to drought stress. Adira 1 and Kaspro are thought as drought-tolerant genotypes due to the morphological, physiological, and molecular responses.

scholarly journals AtCRY2 Negatively Regulates the Functions of AtANN2 and AtANN3 in Drought Tolerance by Affecting Their Subcellular Localization and Transmembrane Ca2+ Flow

2021 ◽  
Vol 12 ◽  
Author(s):  
Ting Liu ◽  
Leyan Du ◽  
Qiushi Li ◽  
Jingda Kang ◽  
Qi Guo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Plasma Membrane ◽  
Drought Stress ◽  
Ion Channels ◽  
Drought Tolerance ◽  
Subcellular Localization ◽  
Calcium Binding ◽  
Physiological Responses ◽  
Ion Flow ◽  
Long Day

Annexins are a multifunctional class of calcium-binding proteins in plants, and their physiological functions and regulation in response to drought stress remain to be elucidated. Here, we found that AtANN2 and AtANN3 conferred to drought tolerance under short-day and long-day conditions, respectively. Under their functional photoperiod, AtANN2 and AtANN3 gene expression was enhanced in the mannitol-treated roots, and their encoded proteins were rapidly targeted to the plasma membrane, and mediated significant Ca2+ flows across the plasma membrane. Cryptochromes as photoreceptors can not only sense the photoperiod and regulate ion channels on the plasma membrane to influence ion flow but also induce downstream physiological responses. AtCRY2 repressed the functions of AtANN2 and AtANN3 by affecting their plasma membrane localization and inhibited AtANN2- and AtANN3-dependent transmembrane Ca2+ flow in response to drought stress. Taken together, these results uncover a mechanism linking Annexins-AtCRY2 to transmembrane Ca2+ flow and resulting in enhanced drought tolerance in Arabidopsis.

Respon Morfologis dan Ekspresi Gen Aquaporin pada Padi IR 64 yang Mengalami Cekaman Kekeringan pada Fase Reproduktif (Morphological Responses and Aquaporin Gene Expression in Rice IR64 under Drought Stress at the Reproductive Stage)

2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Made Pharmawati ◽  
Ni Nyoman Wirasiti ◽  
Luh Putu Wrasiati
Keyword(s):  
Gene Expression ◽  
Drought Stress ◽  
Real Time ◽  
Real Time Pcr ◽  
Control Plant ◽  
Reproductive Stage ◽  
Limiting Factors ◽  
Rice Grain ◽  
Aquaporin Gene ◽  
Peg Treatment

Abstrak Cekaman kekeringan merupakan faktor pembatas penting bagi pertumbuhan dan produktivitas tanaman termasuk padi.      Penelitian ini bertujuan menganalisis respon padi IR64 terhadap cekaman kekeringan dengan pemberian polietilen glikol (PEG) pada fase reproduktif.  Penelitian juga bertujuan menganalisis ekspresi gen aquaporin akibat cekaman kekeringan.  Bibit padi ditanam dalam pot dan perlakuan PEG dengan konsentrasi 108g/L (-0.25MPa) dan 178g/L (-0.52 MPa) diberikan saat munculnya panikula. Perlakuan diberikan selama 2 minggu, kemudian tanaman disiram kembali.  Ekspresi gen diamati pada akhir perlakuan dengan semi kuantitatif real time PCR.  Ekstraksi RNA menggunakan RNeasy plant mini kit, sedangkan sintesis cDNA menggunakan Transcriptor First Strand cDNA Kit.  Hasil penelitian menunjukkan bahwa jumlah malai dan berat total malai berkurang akibat cekaman kekeringan.  Persentase gabah kosong mencapai 84,6% pada perlakuan PEG-0,52 MPa, sedangkan pada perlakuan PEG -0,25 MPa persentase gabah kosong sebesar 67,8%.  Pada kontrol persentase gabah kosong adalah 10,3%.  Ekspresi gen OsPIP2;7 sedikit menurun pada perlakuan PEG -0,52 MPa.Kata kunci: ekspresi gen, IR64, kekeringan, padi, PEG  Abstract Drought stress is one of the limiting factors of plant growth and productivity including rice.  The aim of this study was to analyze responses of IR64 rice to polyethylene glycol (PEG)-induced-drought stress at the reproductive stage.  This study also aimed to analyze the expression of aquaporin under drought stress.  Rice seedlings were grown in pot system and PEG treatment at concentration of -0.25MPa (108g/L) and -0.52 MPa (178g/L) were given when the panicles arose.  Treatments were conducted for 2 weeks, after that the plants were rewatered.  Gene expression was evaluated at the end of PEG treatment using semi quantitative real time PCR. RNA was extracted using RNeasy plant mini kit, while cDNA synthesis was done using Transcriptor First Strand cDNA Kit.  The results showed that the number and weight of rice ear were less in plant treated with PEG than in control.  The percentage of empty rice grain reached 84.6% at PEG -0.52 MPa, while at PEG -0.25 MPa the percentage of empty grain was 67.8%.  In control plant, the percentage of empty grain was 10.3%.  Drought stress did not alter the expression of OsPIP2;7.  Keywords: drought, gene expression, IR64, PEG, rice

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