Collagen types IX and X in the developing chick tibiotarsus: analyses of mRNAs and proteins

Development ◽  
1991 ◽  
Vol 111 (1) ◽  
pp. 191-196 ◽  
Author(s):  
T.F. Linsenmayer ◽  
Q.A. Chen ◽  
E. Gibney ◽  
M.K. Gordon ◽  
J.K. Marchant ◽  
...  
Keyword(s):  
Translational Regulation ◽  
Type Ii ◽  
Embryonic Chick ◽  
Time Data ◽  
Type X Collagen ◽  
Collagen Types ◽  
Marrow Cavity ◽  
Cartilage Development ◽  
Collagen Protein

To examine the regulation of collagen types IX and X during the hypertrophic phase of endochondral cartilage development, we have employed in situ hybridization and immunofluorescence histochemistry on selected stages of embryonic chick tibiotarsi. The data show that mRNA for type X collagen appears at or about the time that we detect the first appearance of the protein. This result is incompatible with translational regulation, which would require accumulation of the mRNA to occur at an appreciably earlier time. Data on later-stage embryos demonstrate that once hypertrophic chondrocytes initiate synthesis of type X collagen, they sustain high levels of its mRNA during the remainder of the hypertrophic program. This suggests that these cells maintain their integrity until close to the time that they are removed at the advancing marrow cavity. Type X collagen protein in the hypertrophic matrix also extends to the marrow cavity. Type IX collagen is found throughout the hypertrophic matrix, as well as throughout the younger cartilaginous matrices. But the mRNA for this molecule is largely or completely absent from the oldest hypertrophic cells. These data are consistent with a model that we have previously proposed in which newly synthesized type X collagen within the hypertrophic zone can become associated with type II/IX collagen fibrils synthesized and deposited earlier in development (Schmid and Linsenmayer, 1990; Chen et al. 1990).

scholarly journals Quantitative analysis of type X-collagen biosynthesis by embryonic-chick sternal cartilage

1986 ◽  
Vol 233 (2) ◽  
pp. 357-367 ◽  
Author(s):  
S A Jimenez ◽  
R Yankowski ◽  
A M Reginato
Keyword(s):  
Quantitative Analysis ◽  
Hyaline Cartilage ◽  
Type Ii Collagen ◽  
Type Ii ◽  
Embryonic Chick ◽  
Collagen Biosynthesis ◽  
Molecular Aggregate ◽  
Type X Collagen ◽  
Organ Cultures ◽  
Disulphide Bonds

We have performed a quantitative analysis of the various collagens biosynthesized by organ cultures of whole embryonic-chick sternum and its separate anatomical regions corresponding to the zones of permanent hyaline and presumptive-calcification cartilages. Our studies demonstrated that embryonic-chick sternum devotes a large portion of its biosynthetic commitment towards production of Type X collagen, which represented approx. 18% of the total newly synthesized collagen. Comparison of the collagens biosynthesized by the permanent hyaline cartilage and by the cartilage from the presumptive-calcification zone demonstrated that Type X-collagen production was strictly confined to the presumptive-calcification region. Sequential extraction of the newly synthesized Type X collagen demonstrated the existence of two separate populations. One population (approx. 20%) was composed of easily extractable molecules that were solubilized with 1.0 m-NaCl/50 mM-Tris/HCI buffer, pH 7.4. The second population was composed of molecules that were not extractable even after repeated pepsin digestion, but became completely solubilized after treatment with 20 mM-dithiothreitol/0.15 M-NaCl buffer at neutral pH. These results suggest that most of the Type X collagen normally exists in the tissue as part of a pepsin-resistant molecular aggregate that may be stabilized by disulphide bonds. Quantitative analysis of the proportion of Type X collagen relative to the other collagens synthesized in the cultures indicated that this collagen was a major biosynthetic product of the presumptive-calcification cartilage, since it represented about 35% of the total collagen synthesized by this tissue. In contrast, the permanent hyaline cartilage did not display any detectable synthesis of Type X collagen. When compared on a per-cell basis, the chondrocytes from the presumptive-calcification zone synthesized approx. 33% more Type X collagen than the amount of Type II collagen synthesized by the chondrocytes from the permanent-hyaline-cartilage zone. Subsequently, it was demonstrated that Type X collagen is a structural component of chick sternum matrix, since quantitative amounts could be extracted from the region of presumptive calcification of 17-day-old chick-embryo sterna and from the calcified portion of adult-chick sterna. The strict topographic distribution in the expression of Type X collagen biosynthesis to the zone of presumptive calcification suggests that this collagen may play an important role in initiation or progression of tissue calcification.

scholarly journals Differential localization of mRNAs of collagen types I and II in chick fibroblasts, chondrocytes, and corneal cells by in situ hybridization using cDNA probes.

1986 ◽  
Vol 102 (6) ◽  
pp. 2302-2309 ◽  
Author(s):  
M Hayashi ◽  
Y Ninomiya ◽  
J Parsons ◽  
K Hayashi ◽  
B R Olsen ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
In Situ Hybridization ◽  
Epithelial Cells ◽  
Type Ii Collagen ◽  
Type I ◽  
Type Ii ◽  
Collagen Types ◽  
Paraffin Sections ◽  
Extracellular Matrix Components

We have employed a highly specific in situ hybridization protocol that allows differential detection of mRNAs of collagen types I and II in paraffin sections from chick embryo tissues. All probes were cDNA restriction fragments encoding portions of the C-propeptide region of the pro alpha-chain, and some of the fragments also encoded the 3'-untranslated region of mRNAs of either type I or type II collagen. Smears of tendon fibroblasts and those of sternal chondrocytes from 17-d-old chick embryos as well as paraffin sections of 10-d-old whole embryos and of the cornea of 6.5-d-old embryos were hybridized with 3H-labeled probes for either type I or type II collagen mRNA. Autoradiographs revealed that the labeling was prominent in tendon fibroblasts with the type I collagen probe and in sternal chondrocytes with the type II collagen probe; that in the cartilage of sclera and limbs from 10-d-old embryos, the type I probe showed strong labeling of fibroblast sheets surrounding the cartilage and of a few chondrocytes in the cartilage, whereas the type II probe labeled chondrocytes intensely and only a few fibroblasts; and that in the cornea of 6.5-d-old embryos, the type I probe labeled the epithelial cells and fibroblasts in the stroma heavily, and the endothelial cells slightly, whereas the type II probe labeled almost exclusively the epithelial cells except for a slight labeling in the endothelial cells. These data indicate that embryonic tissues express these two collagen genes separately and/or simultaneously and offer new approaches to the study of the cellular regulation of extracellular matrix components.

Connexin expression by alveolar epithelial cells is regulated by extracellular matrix

2001 ◽  
Vol 280 (2) ◽  
pp. L191-L202 ◽  
Author(s):  
Yihe Guo ◽  
Cara Martinez-Williams ◽  
Clare E. Yellowley ◽  
Henry J. Donahue ◽  
D. Eugene Rannels
Keyword(s):  
Extracellular Matrix ◽  
Epithelial Cells ◽  
Gap Junction ◽  
Intercellular Communication ◽  
Translational Regulation ◽  
Alveolar Epithelial Cells ◽  
Alveolar Type ◽  
Type Ii ◽  
Gap Junction Intercellular Communication ◽  
Type Ii Cell

Extracellular matrix (ECM) proteins promote attachment, spreading, and differentiation of cultured alveolar type II epithelial cells. The present studies address the hypothesis that the ECM also regulates expression and function of gap junction proteins, connexins, in this cell population. Expression of cellular fibronectin and connexin (Cx) 43 increase in parallel during early type II cell culture as Cx26 expression declines. Gap junction intercellular communication is established over the same interval. Cells plated on a preformed, type II cell-derived, fibronectin-rich ECM demonstrate accelerated formation of gap junction plaques and elevated gap junction intercellular communication. These effects are blocked by antibodies against fibronectin, which cause redistribution of Cx43 protein from the plasma membrane to the cytoplasm. Conversely, cells cultured on a laminin-rich ECM, Matrigel, express low levels of Cx43 but high levels of Cx26, reflecting both transcriptional and translational regulation. Cx26 and Cx43 thus demonstrate reciprocal regulation by ECM constituents.

Battery Capacity Estimation From Partial-Charging Data Using Gaussian Process Regression

2017 ◽  
Author(s):  
Robert R. Richardson ◽  
Christoph R. Birkl ◽  
Michael A. Osborne ◽  
David A. Howey
Keyword(s):  
Gaussian Process ◽  
Gaussian Process Regression ◽  
Diagnostic Technique ◽  
Lithium Ion ◽  
Constant Current ◽  
Time Data ◽  
Capacity Estimation ◽  
Voltage Range ◽  
Battery Capacity

Accurate on-board capacity estimation is of critical importance in lithium-ion battery applications. Battery charging/discharging often occurs under a constant current load, and hence voltage vs. time measurements under this condition may be accessible in practice. This paper presents a novel diagnostic technique, Gaussian Process regression for In-situ Capacity Estimation (GP-ICE), which is capable of estimating the battery capacity using voltage vs. time measurements over short periods of galvanostatic operation. The approach uses Gaussian process regression to map from voltage values at a selection of uniformly distributed times, to cell capacity. Unlike previous works, GP-ICE does not rely on interpreting the voltage-time data through the lens of Incremental Capacity (IC) or Differential Voltage (DV) analysis. This overcomes both the need to differentiate the voltage-time data (a process which amplifies measurement noise), and the requirement that the range of voltage measurements encompasses the peaks in the IC/DV curves. Rather, GP-ICE gives insight into which portions of the voltage range are most informative about the capacity for a particular cell. We apply GP-ICE to a dataset of 8 cells, which were aged by repeated application of an ARTEMIS urban drive cycle. Within certain voltage ranges, as little as 10 seconds of charge data is sufficient to enable capacity estimates with ∼ 2% RMSE.

scholarly journals Progress on Coronal, Interplanetary, Foreshock, and Outer Heliospheric Radio Emissions

2000 ◽  
Vol 17 (1) ◽  
pp. 22-34 ◽  
Author(s):  
Iver H. Cairns ◽  
P. A. Robinson ◽  
G. P. Zank
Keyword(s):  
Solar Wind ◽  
Plasma Waves ◽  
Recent Theory ◽  
Type Ii ◽  
Solar Radio Bursts ◽  
Radio Emissions ◽  
Source Regions ◽  
Earth’S Bow Shock ◽  
Made In

AbstractType II and III solar radio bursts are associated with shock waves and streams of energetic electrons, respectively, which drive plasma waves and radio emission at multiples of the electron plasma frequency as they move out from the corona into the interplanetary medium. Analogous plasma waves and radiation are observed from the foreshock region upstream of Earth's bow shock. In situ spacecraft observations in the solar wind have enabled major progress to be made in developing quantitative theories for these phenomena that are consistent with available data. Similar processes are believed responsible for radio emissions at 2–3 kHz that originate in the distant heliosphere, from where the solar wind interacts with the local interstellar medium. The primary goal of this paper is to review the observations and theories for these four classes of emissions, focusing on recent progress in developing detailed theories for the plasma waves and radiation in the source regions. The secondary goal is to introduce and review stochastic growth theory, a recent theory which appears quantitatively able to explain the wave observations in type III bursts and Earth's foreshock and is a natural theory to apply to type II bursts, the outer heliospheric emissions, and perhaps astrophysicalemissions.

scholarly journals In-situ measurement of reactive hydrocarbons at Hohenpeissenberg with comprehensive gas chromatography (GCxGC-FID): use in estimating HO and NO<sub>3</sub>

2006 ◽  
Vol 6 (4) ◽  
pp. 8155-8188
Author(s):  
S. Bartenbach ◽  
J. Williams ◽  
C. Plass-Dülmer ◽  
H. Berresheim ◽  
J. Lelieveld
Keyword(s):  
Mean Value ◽  
Time Data ◽  
Night Time ◽  
Radical Species ◽  
Diurnal Cycles ◽  
Meteorological Observatory ◽  
Variate Analysis ◽  
The Relationship ◽  
Good Agreement

Abstract. During a field campaign at the Meteorological Observatory Hohenpeissenberg (MOHp) in July 2004, VOCs were measured using GCxGC-FID. Comparison to routinely made GC-MS measurements showed good agreement for a variety of anthropogenic and biogenic ambient VOCs ranging in concentration from below the detection limit (0.1 pmol mol−1) to 180 pmol mol−1. Pronounced diurnal cycles were found for both the biogenic and anthropogenic compounds, driven for the most part by the daily rise and fall of the boundary layer over the station. For the reactive compounds (lifetimes <2 days), a significant, non-zero dependency of the variability on lifetime was found, indicating that chemistry (as opposed to transport alone) was playing a role in determining the ambient VOC concentrations. The relationship was exploited using a single-variate analysis to derive a daytime mean value of HO (5.3±1.4×106 molecules cm−3), which compares well to that measured at the site, 3.2±2.3×106 molecules cm−3. The analysis was extended to the night time data to estimate concentrations for NO3 (1.47±0.2×108 molecules cm−3), which is not measured at the site. The feasibility of this approach for environments dominated by emissions of short-lived VOCs to estimate ambient levels of radical species is discussed.

scholarly journals On the Quality of Real-Time Altimeter Gridded Fields: Comparison with In Situ Data

2009 ◽  
Vol 26 (3) ◽  
pp. 556-569 ◽  
Author(s):  
Ananda Pascual ◽  
Christine Boone ◽  
Gilles Larnicol ◽  
Pierre-Yves Le Traon
Keyword(s):  
Real Time ◽  
Sea Level ◽  
Tide Gauge ◽  
Deep Ocean ◽  
Velocity Estimation ◽  
Time Data ◽  
In Situ Data ◽  
Delayed Time

Abstract The timeliness of satellite altimeter measurements has a significant effect on their value for operational oceanography. In this paper, an Observing System Experiment (OSE) approach is used to assess the quality of real-time altimeter products, a key issue for robust monitoring and forecasting of the ocean state. In addition, the effect of two improved geophysical corrections and the number of missions that are combined in the altimeter products are also analyzed. The improved tidal and atmospheric corrections have a significant effect in coastal areas (0–100 km from the shore), and a comparison with tide gauge observations shows a slightly better agreement with the gridded delayed-time sea level anomalies (SLAs) with two altimeters [Jason-1 and European Remote Sensing Satellite-2 (ERS-2)/Envisat] using the new geophysical corrections (mean square differences in percent of tide gauge variance of 35.3%) than those with four missions [Jason-1, ERS/Envisat, Ocean Topography Experiment (TOPEX)/Poseidoninterlaced, and Geosat Follow-On] but using the old corrections (36.7%). In the deep ocean, however, the correction improvements have little influence. The performance of fast delivery products versus delayed-time data is compared using independent in situ data (tide gauge and drifter data). It clearly highlights the degradation of real-time SLA maps versus the delayed-time SLA maps: four altimeters are needed in real time to get the similar quality performance as two altimeters in delayed time (sea level error misfit around 36%, and zonal and meridional velocity estimation errors of 27% and 33%, respectively). This study proves that the continuous improvement of geophysical corrections is very important, and that it is essential to stay above a minimum threshold of four available altimetric missions to capture the main space and time oceanic scales in fast delivery products.

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