Experimental study of the formation of the heart tube in the chick embryo

Development ◽  
1975 ◽  
Vol 33 (1) ◽  
pp. 1-11
Author(s):  
Carlos Argüello ◽  
María V. De La Cruz ◽  
Concepción Sánchez Gómez
Keyword(s):  
Experimental Study ◽  
Methylene Blue ◽  
Iron Oxide ◽  
Chick Embryo ◽  
Serial Section ◽  
Anterior Part ◽  
Time Lapse ◽  
Heart Tube

A study was made of the development of the heart tube beginning from Hamburger & Hamilton (1951) stage 8+ up to stage 12. We used labelling with particles of iron oxide followed with time-lapse cinemicrophotography, staining with methylene blue, serial section and cutting the embryo in two halves. Our results led to the conclusion that the tubular heart is formed by the addition of precardiac material into its posterior end, but in addition it is necessary to consider the fusion of the myocardium in a cephalic direction, starting with the fusion of both heart primordia at the rostral end. By this fusion the most anterior part of the heart up to stage 12 is formed.

The effect on the intestines of continuous release of methylene blue from a drug delivery system: an experimental study in a chick embryo gastroschisis model

2004 ◽  
Vol 20 (8) ◽  
pp. 609-613
Author(s):  
�zg�r Denli ◽  
Meral Barlas ◽  
Meltem Bingol-Kologlu ◽  
Aydin Yagmurlu ◽  
?�kr� �zdamar ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Experimental Study ◽  
Methylene Blue ◽  
Chick Embryo ◽  
Drug Delivery System ◽  
Delivery System ◽  
Continuous Release

Fe3O4 Magnetic Nanoparticles: Characterization and Performance Exemplified by the Degradation of Methylene Blue in the Presence of Persulfate

2016 ◽  
Vol 19 (1) ◽  
Author(s):  
Chang-Mao Hung ◽  
Chiu-Wen Chen ◽  
Yu-Zhe Jhuang ◽  
Cheng-Di Dong
Keyword(s):  
Methylene Blue ◽  
Iron Oxide ◽  
Magnetic Nanoparticles ◽  
Fe3o4 Magnetic Nanoparticles ◽  
Iron Oxide Magnetic Nanoparticles ◽  
Nanoparticles Characterization ◽  
And Performance

AbstractIn this study, the oxidation of methylene blue (MB) over iron oxide magnetic nanoparticles (Fe

scholarly journals Magnetically separable reduced graphene oxide/iron oxide nanocomposite materials for environmental remediation

2014 ◽  
Vol 4 (12) ◽  
pp. 4396-4405 ◽  
Author(s):  
Teo Peik-See ◽  
Alagarsamy Pandikumar ◽  
Lim Hong Ngee ◽  
Huang Nay Ming ◽  
Chia Chin Hua
Keyword(s):  
Methylene Blue ◽  
Graphene Oxide ◽  
Iron Oxide ◽  
Photocatalytic Degradation ◽  
Reduced Graphene Oxide ◽  
Environmental Remediation ◽  
Nanocomposite Materials ◽  
Reduced Graphene ◽  
Magnetically Separable

Synthesis of magnetically separable rGO/Fe3O4nanocomposite materials for environmental remediationviathe photocatalytic degradation of methylene blue.

Intelligent modeling and experimental study on methylene blue adsorption by sodium alginate-kaolin beads

2021 ◽  
Author(s):  
Nader Marzban ◽  
Ahmad Moheb ◽  
Svitlana Filonenko ◽  
Seyyed Hossein Hosseini ◽  
Mohammad Javad Nouri ◽  
...  
Keyword(s):  
Experimental Study ◽  
Methylene Blue ◽  
Sodium Alginate ◽  
Methylene Blue Adsorption ◽  
Intelligent Modeling

Primordial germ cells in normal and transected duck blastoderms

Development ◽  
1968 ◽  
Vol 20 (3) ◽  
pp. 247-260
Author(s):  
Teresa Rogulska
Keyword(s):  
Chick Embryo ◽  
Blood Vessels ◽  
Germ Cells ◽  
Anterior Part ◽  
Primordial Germ Cells ◽  
Primitive Streak ◽  
Experimental Investigations ◽  
Suggestive Evidence ◽  
Area Pellucida

Suggestive evidence for the extragonadal origin of germ cells in birds was first presented by Swift (1914), who described primordial germ cells in the chick embryo at as early a stage as the primitive streak. According to Swift, primordial germ cells are originally located extra-embryonically in the anterior part of the blastoderm and occupy a crescent-shaped region (‘germinal crescent’) on the boundary between area opaca and area pellucida. Swift also found that primordial germ cells later enter into the blood vessels, circulate together with the blood throughout the whole blastoderm and finally penetrate into the genital ridges, where they become definitive germ cells. Swift's views have been confirmed in numerous descriptive and experimental investigations. Among the latter, the publications of Willier (1937), Simon (1960) and Dubois (1964a, b, 1965a, b, 1966) merit special attention. Dubois finally proved that the genital ridges exert a strong chemotactic influence on the primordial germ cells.

Contact behaviour during the reassociation of dissociated epithelial cells in primary culture

1987 ◽  
Vol 88 (4) ◽  
pp. 521-526
Author(s):  
R.M. Brown ◽  
C.A. Middleton
Keyword(s):  
Epithelial Cells ◽  
Chick Embryo ◽  
Primary Culture ◽  
Cell Cultures ◽  
Corneal Epithelium ◽  
Time Lapse ◽  
Cell Types ◽  
Epidermal Cells ◽  
Isolated Cells ◽  
Contact Behaviour

The behaviour in culture of dissociated epithelial cells from chick embryo pigmented retina epithelium (PRE), corneal epithelium (CE) and epidermis has been studied using time-lapse cinematography. The analysis concentrated on the contact behaviour of 60 previously isolated cells of each type during a 24 h period starting 3.5 h after the cells were plated out. During the period analysed the number of isolated cells in cultures of all three types gradually decreased as they became incorporated into islands and sheets of cells. However, there were significant differences in behaviour between the cell types during the establishment of these sheets and islands. In PRE cell cultures, islands of cells developed because, throughout the period of analysis, collisions involving previously isolated cells almost invariably resulted in the development of a stable contact. Once having established contact with another cell these cells rarely broke away again to become reisolated. In contrast the contacts formed between colliding CE and epidermal cells were, at least initially, much less stable and cells of both these types were frequently seen to break away and become reisolated after colliding with other cells. Sheets and islands of cells eventually developed in these cultures because the frequency with which isolated cells become reisolated decreased with increasing time in culture. The possible reasons underlying the different behaviour of PRE cells, when compared with that of CE and epidermal cells, are discussed. It is suggested that the decreasing tendency of isolated CE and epidermal cells to become reisolated may be related to the formation of desmosomes.

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