Regeneration and compartments in Drosophila

Development ◽  
1979 ◽  
Vol 49 (1) ◽  
pp. 229-241
Author(s):  
Janos Szabad ◽  
Pat Simpson ◽  
Rolf Nöthiger
Keyword(s):  
Clonal Analysis ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Sensitive Cell ◽  
X Irradiation ◽  
Wing Discs ◽  
Growing Population ◽  
Male Tissue ◽  
Do So

A clonal analysis was performed in order to study the process of regeneration in wounded wing discs of Drosophila. Regeneration was induced either by cutting the disc in situ, or by shifting gynandromorphic larvae whose male tissue was hemizygous for a temperature sensitive cell lethal to the restrictive temperature. Fast growing M+ clones, labelled with y and/or mwh, were produced by X-irradiation of the following genotypes: y; scJ4(y+)M(3)i55/ mwh jv M+ (series I), and l(1)ts 504 sn3l(1)ts 5697/ In(1)w°vC; M(3)i55/mwh M+ (series II). The clones were induced either before or after the experimental lesion. Clones initiated one day prior to the lesion were able to cross compartment boundaries whereas clones initiated one day after the lesion did not do so. It is concluded that cells involved in the process cf regeneration lose their compartmental commitment, but that later on the growing population of cells again becomes subdivided into the same compartments.

scholarly journals Induction of DNA Synthesis by Fibroblast Growth Factor in Temperature-Sensitive Cell-Cycle Mutants of Rat 3Y1 Fibroblasts Arrested at Restrictive Temperature.

1992 ◽  
Vol 17 (1) ◽  
pp. 19-25
Author(s):  
Yoshikazu Umeno ◽  
Atsuyuki Okuda ◽  
Hideo Shimura ◽  
Kazukiyo Onodera ◽  
Genki Kimura
Keyword(s):  
Cell Cycle ◽  
Growth Factor ◽  
Dna Synthesis ◽  
Fibroblast Growth Factor ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Fibroblast Growth ◽  
Sensitive Cell

A clonal analysis of pattern duplication in a temperature-sensitive cell-lethal mutant ofDrosophila melanogaster

1980 ◽  
Vol 77 (1) ◽  
pp. 1-21 ◽  
Author(s):  
Jack R. Girton ◽  
Michael A. Russell
Keyword(s):  
Clonal Analysis ◽  
Temperature Sensitive ◽  
Sensitive Cell ◽  
Lethal Mutant

scholarly journals THE ROLE OF S. CEREVISIAE CELL DIVISION CYCLE GENES IN NUCLEAR FUSION

Genetics ◽  
1982 ◽  
Vol 100 (2) ◽  
pp. 175-184
Author(s):  
Susan K Dutcher ◽  
Leland H Hartwell
Keyword(s):  
Cell Division ◽  
Parent Strain ◽  
Nuclear Fusion ◽  
Cell Division Cycle ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Gene Products ◽  
Sensitive Cell ◽  
Single Lesion

ABSTRACT Forty temperature-sensitive cell division cycle (cdc) mutants of Saccharomyces cerevisiae were examined for their ability to complete nuclear fusion during conjugation in crosses to a CDC parent strain at the restrictive temperature. Most of the cdc mutant alleles behaved as the CDC parent strain from which they were derived, in that zygotes produced predominantly diploid progeny with only a small fraction of zygotes giving rise to haploid progeny (cytoductants) that signalled a failure in nuclear fusion. However, cdc4 mutants exhibited a strong nuclear fusion (karyogamy) defect in crosses to a CDC parent and cdc28, cdc34 and cdc37 mutants exhibited a weak karyogamy defect. For all four mutants, the karyogamy defect and the cell cycle defect cosegregated, suggesting that both defects resulted from a single lesion for each of these cdc mutants. Therefore, the cdc 4, 28, 34 and 37 gene products are required in both cell division and karyogamy.

Inhibition of herpes simplex virus type 1 replication in temperature-sensitive cell cycle mutants.

1978 ◽  
Vol 25 (1) ◽  
pp. 42-50 ◽  
Author(s):  
K Yanagi ◽  
A Talavera ◽  
T Nishimoto ◽  
M G Rush
Keyword(s):  
Cell Cycle ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Temperature Sensitive ◽  
Sensitive Cell ◽  
Simplex Virus

scholarly journals Requirement for Three Novel Protein Complexes in the Absence of the Sgs1 DNA Helicase in Saccharomyces cerevisiae

Genetics ◽  
2001 ◽  
Vol 157 (1) ◽  
pp. 103-118 ◽  
Author(s):  
Janet R Mullen ◽  
Vivek Kaliraman ◽  
Samer S Ibrahim ◽  
Steven J Brill
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genome Stability ◽  
Protein Complexes ◽  
Ring Finger ◽  
Dna Helicase ◽  
Open Reading Frames ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Dna Helicases ◽  
Cell Extracts

Abstract The Saccharomyces cerevisiae Sgs1 protein is a member of the RecQ family of DNA helicases and is required for genome stability, but not cell viability. To identify proteins that function in the absence of Sgs1, a synthetic-lethal screen was performed. We obtained mutations in six complementation groups that we refer to as SLX genes. Most of the SLX genes encode uncharacterized open reading frames that are conserved in other species. None of these genes is required for viability and all SLX null mutations are synthetically lethal with mutations in TOP3, encoding the SGS1-interacting DNA topoisomerase. Analysis of the null mutants identified a pair of genes in each of three phenotypic classes. Mutations in MMS4 (SLX2) and SLX3 generate identical phenotypes, including weak UV and strong MMS hypersensitivity, complete loss of sporulation, and synthetic growth defects with mutations in TOP1. Mms4 and Slx3 proteins coimmunoprecipitate from cell extracts, suggesting that they function in a complex. Mutations in SLX5 and SLX8 generate hydroxyurea sensitivity, reduced sporulation efficiency, and a slow-growth phenotype characterized by heterogeneous colony morphology. The Slx5 and Slx8 proteins contain RING finger domains and coimmunoprecipitate from cell extracts. The SLX1 and SLX4 genes are required for viability in the presence of an sgs1 temperature-sensitive allele at the restrictive temperature and Slx1 and Slx4 proteins are similarly associated in cell extracts. We propose that the MMS4/SLX3, SLX5/8, and SLX1/4 gene pairs encode heterodimeric complexes and speculate that these complexes are required to resolve recombination intermediates that arise in response to DNA damage, during meiosis, and in the absence of SGS1/TOP3.

scholarly journals A genetic screen for temperature-sensitive morphogenesis-defectiveCaenorhabditis elegansmutants

2021 ◽  
Vol 11 (4) ◽  
Author(s):  
Molly C Jud ◽  
Josh Lowry ◽  
Thalia Padilla ◽  
Erin Clifford ◽  
Yuqi Yang ◽  
...  
Keyword(s):  
Cell Shape ◽  
The Body ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Fundamental Biological Process ◽  
Embryonic Morphogenesis ◽  
Cell Shape Changes ◽  
Development Temperature ◽  
Multicellular Organisms ◽  
Shape Changes

AbstractMorphogenesis involves coordinated cell migrations and cell shape changes that generate tissues and organs, and organize the body plan. Cell adhesion and the cytoskeleton are important for executing morphogenesis, but their regulation remains poorly understood. As genes required for embryonic morphogenesis may have earlier roles in development, temperature-sensitive embryonic-lethal mutations are useful tools for investigating this process. From a collection of ∼200 such Caenorhabditis elegans mutants, we have identified 17 that have highly penetrant embryonic morphogenesis defects after upshifts from the permissive to the restrictive temperature, just prior to the cell shape changes that mediate elongation of the ovoid embryo into a vermiform larva. Using whole genome sequencing, we identified the causal mutations in seven affected genes. These include three genes that have roles in producing the extracellular matrix, which is known to affect the morphogenesis of epithelial tissues in multicellular organisms: the rib-1 and rib-2 genes encode glycosyltransferases, and the emb-9 gene encodes a collagen subunit. We also used live imaging to characterize epidermal cell shape dynamics in one mutant, or1219ts, and observed cell elongation defects during dorsal intercalation and ventral enclosure that may be responsible for the body elongation defects. These results indicate that our screen has identified factors that influence morphogenesis and provides a platform for advancing our understanding of this fundamental biological process.

scholarly journals THE SELECTION OF S. CEREVISIAE MUTANTS DEFECTIVE IN THE START EVENT OF CELL DIVISION

Genetics ◽  
1980 ◽  
Vol 95 (3) ◽  
pp. 561-577 ◽  
Author(s):  
Steven I Reed
Keyword(s):  
Cell Division ◽  
Genetic Analysis ◽  
Linkage Map ◽  
Nuclear Genes ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Mating Pheromone ◽  
Preliminary Characterization ◽  
Complementation Groups ◽  
Selection Of

ABSTRACT Thirty-three temperature-sensitive mutations defective in the start event of the cell division cycle of Saccharomyces cereuisiae were isolated and subjected to preliminary characterization. Complementation studies assigned these mutations to four complementation groups, one of which, cdc28, has been described previously. Genetic analysis revealed that these complementation groups define single nuclear genes, unlinked to one another. One of the three newly identified genes, cdc37, has been located in the yeast linkage map on chromosome IV, two meiotic map units distal to hom2.—Each mutation produces stage-specific arrest of cell division at start, the same point where mating pheromone interrupts division. After synchronization at start by incubation at the restrictive temperature, the mutants retain the capacity to enlarge and to conjugate.

Some Palaeoliths from South Arabia

1954 ◽  
Vol 19 (2) ◽  
pp. 189-218 ◽  
Author(s):  
G. Caton-Thompson
Keyword(s):  
Geographical Society ◽  
South Arabia ◽  
First Time ◽  
Do So

The material here described was found in the Hadhramaut by Elinor Gardner and myself between November 1937 and March 1938. My personal investigation of the Palaeolithic Age was limited by pre-Islamic excavations, and I am therefore indebted to her for the gathering of most of the specimens in situ in terrace gravels, and to her detailed study of their positions.The collection consists mainly of groups from four fairly widely separated localities; the physiography of these has already been outlined in a comprehensive paper published in the Geographical Journal. Whenever appropriate to the purpose of this account, which is to place for the first time on illustrated record all we observed about the palaeoliths, I have reused in this different context illustrations of Quaternary environment which appeared in that Journal. With thanks I acknowledge the permission of the Royal Geographical Society to do so.

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