Cell wall development of Micrasterias americana, especially in isotonic and hypertonic solutions

1976 ◽  
Vol 21 (3) ◽  
pp. 617-631
Author(s):  
K. Ueda ◽  
S. Yoshioka
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Close Contact ◽  
Light And Electron Microscopy ◽  
Wall Layer ◽  
Wall Material ◽  
Cell Wall Development ◽  
Wall Growth ◽  
Wall Materials ◽  
Main Component

The cell wall development of Micrasterias americana was investigated by light and electron microscopy. From digestion experiments with pectinase and cellulase, and from fluorescence spectra in Calcofluor and Coriphosphin solution, it was concluded that pectin substances were the main component of the young developing cell wall and that cellulose was synthesized after the daughter hemicell was well developed. In 0-16 M mannitol, wall materials accumulated and were incompletely incorporated into the wall at the region where wall growth would be expected. The plasma membrane was in close contact with the cell wall at the sinus, and this contact was assumed to prevent penetration of wall material at this region, resulting in the accumulation of wall material at regions other than the sinus. The cellulosic wall layer was formed after the production of pectic substances in the 0-16 M mannitol. In 0-3 M mannitol neither a definite wall layer of cellulose nor a pectic wall was produced, presumably due to extensive dilution of the wall materials in the plasmolysed space between the cell wall and the plasma membrane. Under normal circumstances, the shape of the daughter cell is assumed to be determined by the shape of the developed primary wall, which is induced by precocious differentiation of the wall at the sinus.

Cell wall structures of Epicoccum nigrum (Hyphomycetes)

1973 ◽  
Vol 51 (5) ◽  
pp. 1071-1073 ◽  
Author(s):  
J. A. Brushaber ◽  
R. H. Haskins
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Outer Layer ◽  
Secondary Wall ◽  
Outer Wall ◽  
Wall Layer ◽  
Wall Material ◽  
Primary Wall ◽  
Electron Dense Material ◽  
Wall Structures

Two structurally distinct types of secondary wall layers are present in older hyphae in addition to the primary wall. A coarsely fibrous outer wall layer often becomes quite massive and frequently fuses with the outer wall layers of adjacent cells in the formation of hyphal strands. The uneven deposition of this outer layer often produces large verrucosities. The inner secondary wall layer is relatively electron transparent and contains a reticulum of electron-dense lines. The interface of the inner secondary wall with the cytoplasm is often very irregular, and sections of the plasma membrane are frequently overlain by wall material. The outer secondary wall of conidia is composed of an electron-dense material different from that of the outer wall of hyphae. Cells in the multicellular conidia tend to be polyhedral in shape with either very thick primary walls or thin primary walls having a thick inner wall deposit.

Multivesicular structures and cell wall growth

1969 ◽  
Vol 47 (12) ◽  
pp. 1873-1877 ◽  
Author(s):  
L. C. Fowke ◽  
George Setterfield
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Physiological State ◽  
Plant Cells ◽  
Wall Material ◽  
Golgi Bodies ◽  
Wall Growth ◽  
Cell Wall Deposition ◽  
Applied Auxin ◽  
In Cells

Applied auxin caused cells of artichoke tuber slices to expand and deposit significant amounts of new wall material while cells in slices held on water remained essentially inert in both respects. Cells in all physiological treatments showed multivesicular structures at the plasma membrane (plasmalemmasomes, lomasomes), within the cytoplasm and within the central vacuoles. The number of plasmalemmasomes was considerably greater in cells not depositing wall than in cells treated with auxin to stimulate wall synthesis. Multivesicular structures showed no relation to Golgi bodies, which increase in number and apparent activity in response to auxin treatment. It is concluded that plasmalemmasomes are not involved in cell wall deposition. Multivesicular structures in plant cells could have several origins and it is suggested that some may represent artifactual reorganization of plasmalemma and tonoplast membranes during cytological processing. Such reorganization would presumably be sensitive to the physiological state of the tissue.

scholarly journals Cell wall biosynthesis and the molecular mechanism of plant enlargement

2009 ◽  
Vol 36 (5) ◽  
pp. 383 ◽  
Author(s):  
John S. Boyer
Keyword(s):  
Cell Wall ◽  
Critical Level ◽  
Turgor Pressure ◽  
Chara Corallina ◽  
Wall Material ◽  
Primary Wall ◽  
Terrestrial Plants ◽  
Green Plants ◽  
Wall Deposition ◽  
Wall Growth

Recently discovered reactions allow the green alga Chara corallina (Klien ex. Willd., em. R.D.W.) to grow well without the benefit of xyloglucan or rhamnogalactan II in its cell wall. Growth rates are controlled by polygalacturonic acid (pectate) bound with calcium in the primary wall, and the reactions remove calcium from these bonds when new pectate is supplied. The removal appears to occur preferentially in bonds distorted by wall tension produced by the turgor pressure (P). The loss of calcium accelerates irreversible wall extension if P is above a critical level. The new pectate (now calcium pectate) then binds to the wall and decelerates wall extension, depositing new wall material on and within the old wall. Together, these reactions create a non-enzymatic but stoichiometric link between wall growth and wall deposition. In green plants, pectate is one of the most conserved components of the primary wall, and it is therefore proposed that the acceleration-deceleration-wall deposition reactions are of wide occurrence likely to underlie growth in virtually all green plants. C. corallina is one of the closest relatives of the progenitors of terrestrial plants, and this review focuses on the pectate reactions and how they may fit existing theories of plant growth.

Ultrastructure of a marine psychrophilic Vibrio

1970 ◽  
Vol 16 (11) ◽  
pp. 1027-1031 ◽  
Author(s):  
S. F. Kennedy ◽  
R. R. Colwell ◽  
G. B. Chapman
Keyword(s):  
Electron Microscopy ◽  
Cell Wall ◽  
Plasma Membrane ◽  
Cell Division ◽  
Wall Material ◽  
Cross Wall ◽  
Growth Stages ◽  
Culture Age ◽  
Primary Mechanism ◽  
Age Studies

The structure of Vibrio marinus strain PS-207 was studied by both phase and electron microscopy. It was found to possess a trilaminar plasma membrane and cell wall. Membrane-bounded subunits containing DNA-like material were found dispersed throughout the cytoplasm. Giant round forms or "macrospheres" were observed in all growth stages. The size, shape, and construction of the "macrospheres" showed some variation, but could not be related to culture age. Studies of cell division in V. marinus strain PS-207 indicate the primary mechanism to be a synthesis and centripetal deposition of plasma membrane with a concomitant or subsequent synthesis and centripetal deposition of cross wall material.

scholarly journals Ultrastructural plasma membrane asymmetries in tension and curvature promote yeast cell fusion

2021 ◽  
Author(s):  
Olivia Muriel ◽  
Laetitia Michon ◽  
Wanda Kukulski ◽  
Sophie G Martin
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Sexual Reproduction ◽  
Membrane Fusion ◽  
Cell Fusion ◽  
Turgor Pressure ◽  
Wall Material ◽  
M Cells ◽  
P Cells ◽  
Cell Cell

Cell-cell fusion is central to the process of fertilization for sexual reproduction. This necessitates the remodeling of peri-cellular matrix or cell wall material and the merging of plasma membranes. In walled fission yeast S. pombe, the fusion of P and M cells during sexual reproduction relies on the fusion focus, an actin structure that concentrates glucanase-containing secretory vesicles for local cell wall digestion necessary for membrane fusion. Here, we present a correlative light and electron microscopy (CLEM) quantitative study of a large dataset of 3D tomograms of the fusion site, which revealed the ultrastructure of the fusion focus as an actin-containing, vesicle-dense structure excluding other organelles. Unexpectedly, the data revealed asymmetries between the two gametes: M-cells exhibit a taut and convex plasma membrane that progressively protrudes into P-cells, which exhibit a more slack, wavy plasma membrane. These asymmetries are relaxed upon plasma membrane fusion, with observations of ramified pores that may result from multiple initiations or inhomogeneous expansion. We show that P-cells have a higher exo- to endocytosis ratio than M-cells, and that local reduction in exocytosis abrogates membrane waviness and compromises cell fusion significantly more in P- than M-cells. Reciprocally, reduction of turgor pressure specifically in M-cells prevents their protrusions into P-cells and delays cell fusion. Thus, asymmetric membrane conformations, which result from differential turgor pressure and exocytosis/endocytosis ratios between mating types, favor cell-cell fusion.

scholarly journals PrkA controls peptidoglycan biosynthesis through the essential phosphorylation of ReoM

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Sabrina Wamp ◽  
Zoe J Rutter ◽  
Jeanine Rismondo ◽  
Claire E Jennings ◽  
Lars Möller ◽  
...  
Keyword(s):  
Cell Wall ◽  
Listeria Monocytogenes ◽  
Cell Walls ◽  
Proteolytic Degradation ◽  
Peptidoglycan Biosynthesis ◽  
Wall Growth ◽  
Essential Enzyme ◽  
Main Component ◽  
Kinase Substrates ◽  
Pasta Domain

Peptidoglycan (PG) is the main component of bacterial cell walls and the target for many antibiotics. PG biosynthesis is tightly coordinated with cell wall growth and turnover, and many of these control activities depend upon PASTA-domain containing eukaryotic-like serine/threonine protein kinases (PASTA-eSTK) that sense PG fragments. However, only a few PG biosynthetic enzymes are direct kinase substrates. Here, we identify the conserved ReoM protein as a novel PASTA-eSTK substrate in the Gram-positive pathogen Listeria monocytogenes. Our data show that the phosphorylation of ReoM is essential as it controls ClpCP-dependent proteolytic degradation of the essential enzyme MurA, which catalyses the first committed step in PG biosynthesis. We also identify ReoY as a second novel factor required for degradation of ClpCP substrates. Collectively, our data imply that the first committed step of PG biosynthesis is activated through control of ClpCP protease activity in response to signals of PG homeostasis imbalance.

Microtubules and cell wall development in differentiating protophloem sieve elements of Triticum aestivum L

1987 ◽  
Vol 87 (4) ◽  
pp. 595-607
Author(s):  
E. P. ELEFTHERIOU
Keyword(s):  
Cell Wall ◽  
Triticum Aestivum L ◽  
Cellulose Microfibrils ◽  
Wall Material ◽  
Wall Thickening ◽  
Sieve Elements ◽  
Material Deposition ◽  
Cellulose Microfibril Orientation ◽  
Cell Wall Development ◽  
Developing Area

The densities of microtubules (MTs) along the lateral walls of developing sieve elements in root protophloem of wheat have been investigated by electron microscopy. They increase gradually in the very young sieve elements to reach a maximum just before the initiation of wall thickening. During wall increment MTs remain at high densities (more than 10 MTs μm−1), but their number declines abruptly when wall material deposition ceases. Cell wall thickening is not uniform: broad ridges alternate with narrow depressions, the latter occupied by plasmodesmata. During wall material deposition MTs overlie the thickenings only, being entirely absent from the non-thickened areas. The orientation of MTs reflects that of the currently deposited cellulose microfibrils in the cell wall, all being perpendicular to the direction of cell expansion. Numerous vesicles, apparently of Golgi apparatus origin, are encountered amongst the cortical arrays of MTs. Though the least spacing between the contiguous MTs is much smaller than the diameter of even the smallest vesicles, the latter were seen amongst the MTs, indicating that MTs do not prevent the vesicles from passing between them towards the developing area. All results favour the suggestion that MTs in sieve elements are involved in cell wall pattern development, cellulose microfibril orientation, and presumably in cell elongation.

scholarly journals The Mode of Cell Wall Growth in Selected Archaea Is Similar to the General Mode of Cell Wall Growth in Bacteria as Revealed by Fluorescent Dye Analysis

2010 ◽  
Vol 77 (5) ◽  
pp. 1556-1562 ◽  
Author(s):  
Reinhard Wirth ◽  
Annett Bellack ◽  
Markus Bertl ◽  
Yvonne Bilek ◽  
Thomas Heimerl ◽  
...  
Keyword(s):  
Cell Wall ◽  
Fluorescent Dyes ◽  
Pyrococcus Furiosus ◽  
Wall Material ◽  
Cell Wall Material ◽  
Cell Wall Growth ◽  
Archaeal Species ◽  
Dye Analysis ◽  
Wall Growth ◽  
Methanopyrus Kandleri

ABSTRACTThe surfaces of 8 bacterial and 23 archaeal species, including many hyperthermophilicArchaea, could be stained using succinimidyl esters of fluorescent dyes. This allowed us for the first time to analyze the mode of cell wall growth inArchaeaby subculturing stained cells. The data obtained show that incorporation of new cell wall material inArchaeafollows the pattern observed forBacteria: in the coccoid speciesPyrococcus furiosusincorporation was in the region of septum formation while for the rod-shaped speciesMethanopyrus kandleriandMethanothermus sociabilis, a diffuse incorporation of cell wall material over the cell length was observed. Cell surface appendages like fimbriae/pili, fibers, or flagella were detectable by fluorescence staining only in a very few cases although their presence was proven by electron microscopy. Our data in addition prove that Alexa Fluor dyes can be used forin situanalyses at temperatures up to 100°C.

Light and electron microscopy of the spermogonial stage of Gymnoconia peckiana, one of the causes of orange rust of Rubus

Botany ◽  
2008 ◽  
Vol 86 (5) ◽  
pp. 533-538 ◽  
Author(s):  
Charles W. Mims ◽  
Elizabeth A. Richardson
Keyword(s):  
Cell Wall ◽  
Epidermal Cell ◽  
Leaf Surface ◽  
Light And Electron Microscopy ◽  
Epidermal Cells ◽  
Wall Material ◽  
Cell Wall Material ◽  
Leaf Epidermis ◽  
Single Nucleus ◽  
Epidermal Cell Wall

Hyphae of Gymnoconia peckiana (Howe in Peck) Trotter spread from infected Rubus argutus Link. stems into leaf primordia where they proliferated in an intercellular fashion as leaves differentiated. Hyphae were septate, and each compartment appeared to contain a single nucleus. Hyphae gave rise to numerous haustoria that resembled the monokaryotic haustoria of other rust fungi. Hyphae located immediately adjacent to the upper and lower leaf epidermis gave rise to spermogonial initials. Each initial consisted of a small group of tightly packed hyphae that developed in an intercellular space adjacent to the epidermis. As an initial enlarged, the proliferating hyphae pushed their way between, as well as into, epidermal cells. Invaded epidermal cells soon died. A layer of spermatiophores then developed within each young spermogonium and appeared to push the epidermal cell wall material and leaf cuticle covering the spermogonium out from the leaf surface. Once mature, spermatiophores gave rise to a succession of uninucleate spermatia that emerged from the tip of each spermatiophore. Spermatia initially accumulated beneath the layer of epidermal cell wall material and cuticle that covered the developing spermogonium and appeared to push this layer further out from the leaf surface until it ruptured. A few receptive hyphae were observed in mature spermogonia.

scholarly journals Dynamics of structural polysaccharides deposition on the plasma-membrane surface of plant protoplasts during cell wall regeneration

2019 ◽  
Vol 65 (1) ◽  
Author(s):  
Satomi Tagawa ◽  
Yusuke Yamagishi ◽  
Ugai Watanabe ◽  
Ryo Funada ◽  
Tetsuo Kondo
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Stress Condition ◽  
Laser Scanning Microscopy ◽  
Primary Cell ◽  
Wall Material ◽  
Primary Cell Wall ◽  
Cell Wall Regeneration ◽  
Plant Protoplasts ◽  
Wall Regeneration

Abstract In this study, dynamic changes in structural polysaccharide deposition on the plasma membrane and cortical microtubules (CMTs) behavior were monitored in protoplasts isolated from white birch callus using confocal laser scanning microscopy and atomic force microscopy. We focused on the influence of an environmental stimulus on cell wall regeneration in protoplasts by employing an acidic culture medium containing a high concentration of Ca2+ (the stress condition). Under the non-stress condition, cellulose microfibrils and callose were initially synthesized, and thereafter deposited on the plasma membrane as “primary cell wall material”. Under the stress condition, callose micro-sized fibers were secreted without cell wall regeneration. Behavior of CMTs labeled with mammalian microtubule-associated protein 4 with green fluorescent protein in transgenic protoplasts was monitored by time-lapse video analysis. Under the non-stress condition, CMTs behavior showed a linear arrangement at a fixed position, whereas unfixed manner of CMTs behavior was observed under the stress condition. These findings indicate that excessive Ca2+ affects cellulose synthesis and CMTs dynamics in plant protoplasts. Current study first demonstrated dynamics of cell wall regeneration and CMTs in woody protoplast, which provides novel insight to aid in understanding early stages of primary cell wall formation in plants.

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