Characterization of Human Bone Marrow Niches with Metabolome and Transcriptome Profiling
Bone marrow (BM) niches are special microenvironments that work in harmony with each other for the regulation and maintenance of hematopoiesis. The niche investigations have been remained limited with various model organisms and animal studies until today, therefore, little is known about different niches in healthy humans. In this study, the presence of different niches in BM by special harvesting method for the collection of BM from two different anatomical regions was investigated in the iliac crest of humans and identified metabolomic and transcriptomic profiles using comparative omic technologies. The main cellular pathways and corresponding transcripts and metabolites were identified. As a result, we found that the energy metabolism between the regions is different. This study provides basic broad data for regenerative medicine in terms of the design of the appropriate microenvironment for in-vitro hematopoietic niche modeling or providing the normal reference values that can be compared in hematological disease.