scholarly journals Intracellular flow cytometric lipid analysis: A multiparametric system to assess distinct lipid classes in live cells

2021 ◽  
Author(s):  
Badwi B. Boumelhem ◽  
Chelsea Pilgrim ◽  
Vincent E. Zwicker ◽  
Jacek L. Kolanowski ◽  
Jia Hao Yeo ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Lipid Content ◽  
Mammalian Cells ◽  
Unsaturated Fatty Acids ◽  
Lipid Analysis ◽  
Nile Blue ◽  
Nile Red ◽  
Lipid Classes ◽  
Carbon Chain Length ◽  
Flow Cytometric

The lipid content of mammalian cells varies greatly between cell type. Current methods for analysing lipid components of cells are technically challenging and destructive. Here, we report a facile, inexpensive method to identify lipid content: intracellular flow cytometric lipid analysis (IFCLA). Distinct lipid classes can be distinguished by Nile Blue, Nile Red fluorescence or violet autofluorescence. Nile Blue is fluorescent in the presence of unsaturated fatty acids with a carbon chain length greater than 16. Cis-configured fatty acids induce greater Nile Blue fluorescence than their trans-configured counterparts. In contrast, Nile Red exhibits greatest fluorescence in the presence of cholesterol, cholesteryl esters, some triglycerides and phospholipids. Multiparametric SPADE analysis of hepatic cellular lipid distribution including Vitamin A autofluorescence is presented. This flow cytometric system allows for the rapid, inexpensive, non-destructive identification of lipid content and highlights the differences in lipid biology between cell types by imaging and flow cytometry.

scholarly journals Maternal Lipidomic Signatures in Relation to Spontaneous Preterm Birth and Large-For-Gestational Age Neonates

2021 ◽  
Author(s):  
Max Aung ◽  
Pahriya Ashrap ◽  
Deborah Watkins ◽  
Bhramar Mukherjee ◽  
Zaira Rosario ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Preterm Birth ◽  
Free Fatty Acids ◽  
Gestational Age ◽  
Pregnancy Outcomes ◽  
Lipid Analysis ◽  
Lipid Classes ◽  
Large For Gestational Age ◽  
Spontaneous Preterm Birth ◽  
Increased Risk

Abstract Background: Lipidome-wide metabolites may be useful biomarkers of pregnancy outcomes. We sought to characterize maternal lipidomic signatures associated with preterm birth and neonatal anthropometric parameters. Methods: Plasma samples were collected 24-28 weeks gestation, and lipidomic profiling was quantified using high-performance liquid chromatography tandem mass spectrometry. Lipid metabolites were analyzed individually and as whole lipid classes and subgroups based on degree of hydrocarbon chain saturation. Associations were estimated using linear and logistic regression.Results: After false discovery adjustment (q<0.15), four plasmenyl-phosphatidylethanolamines and three free fatty acids associated with increased risk for spontaneous preterm birth. Five phosphatidylinositols, two phosphatidylglycerols, and one phosphatidic acid were associated with large for gestational age neonates. The saturated plasmenyl-phosphatidylethanolamines held the association with increased risk for spontaneous preterm birth. Both the mono- and poly-unsaturated free fatty acids held the association for increased risk for spontaneous preterm birth. Mono- and poly-unsaturated phosphatidylinositols were associated with large for gestational age neonates. Whole lipid classes (plasmenyl-phophatidylcholines and plasmenyl-phosphatidylethanolamines) were associated with increased risk for large for gestational age at delivery.Conclusions: This study provides evidence that finer omics-scale analysis of the maternal lipidome may be more informative biomarkers of pregnancy outcomes compared to whole class level lipid analysis.

scholarly journals Acute postprandial effect of hydrogenated fish oil, palm oil and lard on plasma cholesterol, triacylglycerol and non-esterified fatty acid metabolism in normocholesterolaemic males

2006 ◽  
Vol 95 (4) ◽  
pp. 787-794 ◽  
Author(s):  
Marie M. Cantwell ◽  
Mary A.T. Flynn ◽  
Michael J. Gibney
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fish Oil ◽  
Palm Oil ◽  
Unsaturated Fatty Acids ◽  
Lipid Analysis ◽  
Plasma Cholesterol ◽  
Random Order ◽  
Postprandial Response ◽  
Chain Fatty Acids

The majority of research has focused on the association betweentransunsaturated fatty acids (TUFA) from hydrogenated vegetable oils and heart disease even though TUFA are also produced from hydrogenated fish oil. We compared the acute effect of three solid fats on postprandial cholesterol, triacylglycerol (TAG) and NEFA concentrations in normocholesterolaemic males. Eight healthy male volunteers consumed each of the three 40g fat meals (partially hydrogenated fish oil (PHFO), palm oil and lard) in random order and blood samples were drawn at 2, 4, 6 and 8h thereafter for lipid analysis. The postprandial response in plasma TAG, TAG-rich lipoprotein-TAG (TRL-TAG), total cholesterol and plasma NEFA, measured as the area under the postprandial curve, was not significantly different between the three meals (p>0·05), which varied in MUFA, PUFA and TUFA content. There was no marked elevation of longer-chain fatty acids (C20–22,cisortransisomers) into the TRL-TAG fraction following the PHFO meal even though they provided 40% of the total fatty acids in the PHFO meal. The postprandial TRL-TAG response to PHFO was expected to be higher, as it is higher in TUFA, lower in PUFA and similar in saturated fatty acid composition compared with the lard and palm oil test meals. The absence of a higher postprandial response following ingestion of PHFO could be as a result of reduced absorption and increased oxidation of long-chain fatty acids (bothcisandtransisomers)

The effect of pantothenate on sulfate metabolism and sulfur isotope fractionation by Saccharomyces cerevisiae

1979 ◽  
Vol 25 (10) ◽  
pp. 1139-1144 ◽  
Author(s):  
R. G. L. McCready ◽  
G. A. Din ◽  
H. R. Krouse
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fatty Acids ◽  
Sulfate Reduction ◽  
Lipid Content ◽  
Isotope Fractionation ◽  
Sulfur Isotope ◽  
Unsaturated Fatty Acids ◽  
Cellular Lipid ◽  
Sulfur Isotope Fractionation ◽  
Sulfate Metabolism

Growth of Saccharomyces cerevisiae in minimal salts – glucose – SO42− medium with varying concentrations of pantothenate (0–1000 μg/L) produced changes in the cellular lipid content and in the ratio of saturated to unsaturated fatty acids. Substantial differences in SO42−diffusion were also observed with changes in pantothenate concentration. During sulfate reduction, the δ34S value of the evolved sulfide varied with the pantothenate concentration ranging from −31‰ in the absence of pantothenate to 0‰ at 400−1000 μg/L pantothenate. The isotope selectivity is related to the effect of pantothenate concentration on cellular metabolism.

Ultrastructure and lipid changes in Pyrenochaeta terrestris during aging

1973 ◽  
Vol 19 (4) ◽  
pp. 491-496 ◽  
Author(s):  
M. Gunasekaran ◽  
W. M. Hess ◽  
D. J. Weber
Keyword(s):  
Fatty Acids ◽  
Lipid Content ◽  
Lipid Analysis ◽  
Synthetic Medium ◽  
Growth Period ◽  
Ultrastructural Level ◽  
Dense Substance ◽  
Total Fatty Acids ◽  
Total Growth ◽  
Pyrenochaeta Terrestris

Pyrenochaeta terrestris, the onion pink root fungus, was grown on a reciprocal shaker in a synthetic medium which contained cellulose as the only carbon source. The mycelium was processed for lipid analysis and ultrastructural investigations after 8, 16, 24, and 32-day growth intervals.Hyphal cells contained membrane complexes. An electron-dense substance was present in large quantities on hyphal cell walls until after the 24-day period. As the cultures aged, organelles in some hyphal cells disintegrated and viable hyphal cells grew inside senescent cells in some cases. It was not possible to correlate positively lipid content observed at the ultrastructural level with the biochemical lipid analysis as a result of the relatively small amount of lipid observed in hyphal cells at the ultrastructural level.The lipid analysis indicated that the lipid content and mycelial weight reached a maximum at the 16-day period. The major fatty acids present were: C16:0, C18:0, C18:1, and C18:2. As aging occurred, the amount of unsaturation increased in the free fatty acids, the total fatty acids, and the fatty acids of the diglycerides and triglycerides. Only the fatty acids of the monoglycerides failed to increase in unsaturation. The sterol content increased slightly over the total growth period.

STUDIES ON THE IN VITRO ANTITUMOR ACTIVITY OF FATTY ACIDS: V. UNSATURATED ACIDS

1962 ◽  
Vol 40 (7) ◽  
pp. 869-875 ◽  
Author(s):  
Susan Tolnai ◽  
Joseph F. Morgan
Keyword(s):  
Fatty Acids ◽  
Antitumor Activity ◽  
Unsaturated Fatty Acids ◽  
Relative Effectiveness ◽  
Carbon Chain ◽  
Ehrlich Carcinoma ◽  
Carbon Chain Length ◽  
Ascites Tumors ◽  
Arachidonic Acids

Previous studies on the in vitro antitumor activity of saturated mono- and dicarboxylic fatty acids have been extended to a series of unsaturated fatty acids ranging in carbon chain length from C6to C20- All 10 compounds tested exhibited some activity at pH 4.0 against the ascites forms of the Ehrlich carcinoma, 6C3HED lymphosarcoma, and TA3mammary carcinoma. Only three compounds (2-decenoic, linoleic, and linolenic acids) were found to be active at neutrality against all three tumors, while four compounds (2-nonenoic, 10-undecenoic, oleic, and arachidonic acids) were active against one or two tumors under neutral conditions. Considerable variations were encountered in the relative effectiveness of these compounds against the three ascites tumors. The compounds with the most marked in vitro antitumor activity were linoleic and linolenic acids.

Studies in the biochemistry of cirripede eggs: II. Changes in lipid composition during development of Balanus balanoides and B. Balanus.

1966 ◽  
Vol 46 (2) ◽  
pp. 249-261 ◽  
Author(s):  
R. M. C. Dawson ◽  
H. Barnes
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Lipid Content ◽  
Cellular Differentiation ◽  
Phosphatidyl Ethanolamine ◽  
Unsaturated Fatty Acids ◽  
Polar Material ◽  
Marine Animals ◽  
Balanus Balanoides ◽  
Late Stages

The eggs of two common cirripedes, Balanus balanoides and B. balanus, have been analysed at different stages of development for their lipid content.Triglyceride and phospholipid are the main lipid components; small amounts of free fatty acids, lipochromes, sterols, and some very non-polar material (possibly hydrocarbon) are also present.Lecithin followed by phosphatidyl ethanolamine make up a large proportion of the phospholipid fraction.The fatty-acid spectrum is similar to that of other marine animals being rich in unsaturated fatty acids of which eicosapentaenoic (20:5) and docosahexaenoic (22:6) predominate.Triglyceride is lost during development.Initially some lecithin and phosphatidyl ethanolamine are lost during development, but they accumulate in the late stages.Cardiolipin shows a marked increase in quantity during the late stages; this is presumably associated with increased cellular differentiation.There is a tendency for all the components to approach the values for the adult bodies—taken when lipid reserves are minimal—as the embryos develop.

Biodiesel and poly-unsaturated fatty acids production from algae and crop plants - a rapid and comprehensive workflow for lipid analysis

2016 ◽  
Vol 11 (10) ◽  
pp. 1262-1267 ◽  
Author(s):  
Takeshi Furuhashi ◽  
Takemichi Nakamura ◽  
Lena Fragner ◽  
Valentin Roustan ◽  
Verena Schön ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Lipid Analysis ◽  
Crop Plants
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