Ductal epithelial cells cultured from human foetal epididymis and vas deferens: relevance to sterility in cystic fibrosis

1989 ◽  
Vol 92 (4) ◽  
pp. 687-690
Author(s):  
A. Harris ◽  
L. Coleman

A tissue culture system for epithelial cells derived from male human foetal genital ducts has been established. The cells show morphological and biochemical characteristics of ductal epithelial cells, and can be passaged and maintained in culture for considerable periods of time. These cells will provide a suitable system for investigating, by electrophysiological, biochemical and molecular biological methods, the cause of sterility in cystic fibrosis.

1987 ◽  
Vol 87 (5) ◽  
pp. 695-703
Author(s):  
A. Harris ◽  
L. Coleman

A tissue culture system for epithelial cells derived from human foetal pancreas has been established. The cultured cells show many ultrastructural features of interlobular duct cells. Immunocytochemical and histochemical evidence is presented in support of the view that these cells are ductal in origin. They are likely to be one of the few cell types that express the basic defect of cystic fibrosis in vitro. The cells may be passaged and sufficient material obtained to permit biochemical and molecular biological analysis.


1988 ◽  
Vol 90 (1) ◽  
pp. 73-77
Author(s):  
A. Harris ◽  
L. Coleman

The establishment of a tissue-culture system for epithelial cells derived from human foetal pancreas has recently been reported. Further analyses have now been made on these cells in vitro, together with parallel investigation of the distribution of different cell types within the intact foetal pancreas. Results support the view that the cultured cells are ductal in origin and nature. Pancreatic epithelial cell cultures have also been established from foetuses with cystic fibrosis.


2014 ◽  
Vol 123 ◽  
pp. 838-844 ◽  
Author(s):  
Dae Seung Kim ◽  
Sang-Myung Jung ◽  
Gwang Heum Yoon ◽  
Hoo Cheol Lee ◽  
Hwa Sung Shin

2020 ◽  
Vol 4 (5) ◽  
pp. 544-559 ◽  
Author(s):  
Thomas von Erlach ◽  
Sarah Saxton ◽  
Yunhua Shi ◽  
Daniel Minahan ◽  
Daniel Reker ◽  
...  

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