A Cytological and Cytochemical Study of the ‘Epithelial Body’ on the Carotid Artery of the Lizards, Trachysaurus Rugosus and Tiliqua Occipitalis

1963 ◽  
Vol s3-104 (66) ◽  
pp. 197-205
Author(s):  
D. C. ROGERS
Keyword(s):  
Epithelial Cell ◽  
Parathyroid Gland ◽  
Clear Cell ◽  
Cell Types ◽  
Chief Cell ◽  
Dark Cell ◽  
Secretory Cycle ◽  
Cytochemical Study ◽  
Cytochemical Investigation ◽  
The Subject

Epithelial body III of Tiliqua occipitalis and Trachysaurus rugosus has been the subject of a histological and cytochemical investigation with the object of making a comparison with the mammalian parathyroid gland. The cytochemical reactions of the reptilian chief cell are similar to those of the mammalian ‘pale principal’ cell. On the basis of a series of cytological and cytochemical changes a secretory cycle commencing with the chief cell and culminating in the water-clear cell is postulated. Two further cell types distinguished as the dark cell and the ‘epithelial’ cell have been described in the reptilian epithelial body. The significance of these two cell types is discussed. There is some evidence suggesting that true sinusoids invest the cellular cords. There is no evidence, with the technique used, that the gland cells are innervated. It is suggested that the general histology is comparable to that of the mammalian parathyroid gland.

Do clear cell ovarian carcinomas have poorer prognosis compared to other epithelial cell types? A study of 1411 clear cell ovarian cancers

2008 ◽  
Vol 109 (3) ◽  
pp. 370-376 ◽  
Author(s):  
John K. Chan ◽  
Deanna Teoh ◽  
Jessica M. Hu ◽  
Jacob Y. Shin ◽  
Kathryn Osann ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Clear Cell ◽  
Cell Types ◽  
Ovarian Carcinomas ◽  
Ovarian Cancers

Ca++-ATPase activity in the hepatopancreas cells of Oniscus asellus

1992 ◽  
Vol 50 (1) ◽  
pp. 820-821
Author(s):  
G.M. Vernon ◽  
A. Surace ◽  
R. Witkus
Keyword(s):  
B Cells ◽  
Epithelial Cell ◽  
Atpase Activity ◽  
Calcium Transport ◽  
Carcinus Maenas ◽  
Cell Types ◽  
Molt Cycle ◽  
Copper And Zinc

The hepatopancreas consists of a pair of bilobed tubules comprised of two epithelial cell types. S cells are absorptive and accumulate metals such as copper and zinc. Ca++ concentrations vary between the S and B cells and during the molt cycle. Roer and Dillaman implicated Ca++-ATPase in calcium transport during molting in Carcinus maenas. This study was undertaken to compare the localization of Ca++-ATPase activity in the S and B cells during intermolt.

Pathogenic Mechanisms Underlying Idiopathic Pulmonary Fibrosis

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Benjamin J. Moss ◽  
Stefan W. Ryter ◽  
Ivan O. Rosas
Keyword(s):  
Epithelial Cell ◽  
Idiopathic Pulmonary Fibrosis ◽  
Pulmonary Fibrosis ◽  
Cell Activation ◽  
Alveolar Epithelial Cell ◽  
Cell Types ◽  
Annual Review ◽  
Publication Date ◽  
Metabolic Dysfunction ◽  
Epithelial Repair

The pathogenesis of idiopathic pulmonary fibrosis (IPF) involves a complex interplay of cell types and signaling pathways. Recurrent alveolar epithelial cell (AEC) injury may occur in the context of predisposing factors (e.g., genetic, environmental, epigenetic, immunologic, and gerontologic), leading to metabolic dysfunction, senescence, aberrant epithelial cell activation, and dysregulated epithelial repair. The dysregulated epithelial cell interacts with mesenchymal, immune, and endothelial cells via multiple signaling mechanisms to trigger fibroblast and myofibroblast activation. Recent single-cell RNA sequencing studies of IPF lungs support the epithelial injury model. These studies have uncovered a novel type of AEC with characteristics of an aberrant basal cell, which may disrupt normal epithelial repair and propagate a profibrotic phenotype. Here, we review the pathogenesis of IPF in the context of novel bioinformatics tools as strategies to discover pathways of disease, cell-specific mechanisms, and cell-cell interactions that propagate the profibrotic niche. Expected final online publication date for the Annual Review of Pathology: Mechanisms of Disease, Volume 17 is January 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

Deficiency in intercellular communication in two established renal epithelial cell lines (LLC-PK1 and MDCK)

1984 ◽  
Vol 66 (1) ◽  
pp. 81-93
Author(s):  
F.V. Sepulveda ◽  
J.D. Pearson
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Mdck Cells ◽  
Cell Communication ◽  
Intercellular Junctions ◽  
Cell Types ◽  
Renal Epithelial Cell ◽  
Aortic Endothelial Cells ◽  
Epithelial Cell Lines ◽  
Autoradiographic Analysis

We have studied the cell-to-cell passage of uridine nucleotides in two renal epithelial cell lines (LLC-PK1 and MDCK) and in porcine aortic endothelial cells (PAE). All three cell types incorporated tritiated uridine. After a 3 h incubation the radioactivity was predominantly in the form of acid-soluble compounds, mainly UTP. Prelabelled LLC-PK1 or MDCK cells were unable to transfer radioactivity to added adjacent, non-labelled cells, whereas PAE cells readily formed communicating intercellular junctions, as judged by autoradiographic analysis after a 3 h co-culture period. Cell-to-cell communication in either of the renal cell lines was not promoted by treatment with dibutyryl cyclic AMP and methylisobutylxanthine. Radioactivity incorporated into the acid-insoluble pool was not available for intercellular transfer, as assessed in experiments in which cells were prelabelled 24 h before co-culture.

Chief cell and clear cell parathyroid adenoma do not influence clinical and biochemical expression of the sporadic primary hyperparathyroidism

Endocrine ◽  
2012 ◽  
Vol 43 (2) ◽  
pp. 440-443 ◽  
Author(s):  
Shweta Varshney ◽  
Sanjay Kumar Bhadada ◽  
Uma Nahar ◽  
Viral N. Shah ◽  
Anil Bhansali ◽  
...  
Keyword(s):  
Primary Hyperparathyroidism ◽  
Parathyroid Adenoma ◽  
Clear Cell ◽  
Chief Cell ◽  
Sporadic Primary Hyperparathyroidism

A novel epithelial cell from neonatal rat lung: isolation and differentiated phenotype

1990 ◽  
Vol 259 (6) ◽  
pp. L415-L425 ◽  
Author(s):  
P. E. Roberts ◽  
D. M. Phillips ◽  
J. P. Mather
Keyword(s):  
Epithelial Cell ◽  
Molecular Mechanisms ◽  
Basal Medium ◽  
Fold Increase ◽  
Cell Types ◽  
Cell Number ◽  
Neonatal Rat ◽  
Rat Lung ◽  
Cell Type

A novel epithelial cell from normal neonatal rat lung has been isolated, established, and maintained for multiple passages in the absence of serum, without undergoing crisis or senescence. By careful manipulation of the nutrition/hormonal microenvironment, we have been able to select, from a heterogeneous population, a single epithelial cell type that can maintain highly differentiated features in vitro. This cell type has characteristics of bronchiolar epithelial cells. A clonal line, RL-65, has been selected and observed for greater than 2 yr in continuous culture. It has been characterized by ultrastructural, morphological, and biochemical criteria. The basal medium for this cell line is Ham's F12/Dulbecco's modified Eagle's (DME) medium plus insulin (1 micrograms/ml), human transferrin (10 micrograms/ml), ethanolamine (10(-4) M), phosphoethanolamine (10(-4) M), selenium (2.5 x 10(-8) M), hydrocortisone (2.5 x 10(-7) M), and forskolin (5 microM). The addition of 150 micrograms/ml of bovine pituitary extract to the defined basal medium stimulates a greater than 10-fold increase in cell number and a 50- to 100-fold increase in thymidine incorporation. The addition of retinoic acid results in further enhancement of cell growth and complete inhibition of keratinization. We have demonstrated a strategy that may be applicable to isolating other cell types from the lung and maintaining their differentiated characteristics for long-term culture in vitro. Such a culture system promises to be a useful model in which to study cellular events associated with differentiation and proliferation in the lung and to better understand the molecular mechanisms involved in these events.

scholarly journals Klasifikasi Sel Darah Putih dan Sel Limfoblas Menggunakan Metode Multilayer Perceptron Backpropagation

2019 ◽  
Vol 9 (2) ◽  
pp. 173
Author(s):  
Apri Nur Liyantoko ◽  
Ika Candradewi ◽  
Agus Harjoko
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Blood Cells ◽  
White Blood Cells ◽  
Back Propagation ◽  
Cell Types ◽  
Diagnostic Process ◽  
The Subject ◽  
Processing Techniques

 Leukemia is a type of cancer that is on white blood cell. This disease are characterized by abundance of abnormal white blood cell called lymphoblast in the bone marrow. Classification of blood cell types, calculation of the ratio of cell types and comparison with normal blood cells can be the subject of diagnosing this disease. The diagnostic process is carried out manually by hematologists through microscopic image. This method is likely to provide a subjective result and time-consuming.The application of digital image processing techniques and machine learning in the process of classifying white blood cells can provide more objective results. This research used thresholding method as segmentation and  multilayer method of back propagation perceptron with variations in the extraction of textural features, geometry, and colors. The results of segmentation testing in this study amounted to 68.70%. Whereas the classification test shows that the combination of feature extraction of GLCM features, geometry features, and color features gives the best results. This test produces an accuration value 91.43%, precision value of 50.63%, sensitivity 56.67%, F1Score 51.95%, and specitifity 94.16%.

scholarly journals An endometrial organoid model of Chlamydia-epithelial and immune cell interactions

2020 ◽  
Author(s):  
Lee Dolat ◽  
Raphael H. Valdivia
Keyword(s):  
Epithelial Cell ◽  
Cell Biology ◽  
Immune Cell ◽  
Three Dimensional ◽  
Time Lapse ◽  
Cell Types ◽  
Infection Process ◽  
Host Cells ◽  
Cell Diversity ◽  
Intracellular Organelles

ABSTRACTOur understanding of how the obligate intracellular bacterium Chlamydia trachomatis reprograms the cell biology of host cells in the upper genital tract is largely based on observations made in cell culture with transformed epithelial cell lines. Here we describe a primary spherical organoid system derived from endometrial tissue to recapitulate epithelial cell diversity, polarity, and ensuing responses to Chlamydia infection. Using high-resolution and time-lapse microscopy, we catalogue the infection process in organoids from invasion to egress, including the reorganization of the cytoskeleton and positioning of intracellular organelles. We show this model is amenable to screening C. trachomatis mutants for defects in the fusion of pathogenic vacuoles, the recruitment of intracellular organelles, and inhibition of cell death. Moreover, we reconstructed a primary immune cell response by co-culturing infected organoids with neutrophils, and determined that the effector TepP limits the recruitment of neutrophils to infected organoids. Collectively, our model details a system to study the cell biology of Chlamydia infections in three dimensional structures that better reflect the diversity of cell types and polarity encountered by Chlamydia upon infection of their animal hosts.Summary statement3D endometrial organoids to model Chlamydia infection and the role of secreted virulence factors in reprogramming host epithelial cells and immune cell recruitment

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