Do clear cell ovarian carcinomas have poorer prognosis compared to other epithelial cell types? A study of 1411 clear cell ovarian cancers

2008 ◽  
Vol 109 (3) ◽  
pp. 370-376 ◽  
Author(s):  
John K. Chan ◽  
Deanna Teoh ◽  
Jessica M. Hu ◽  
Jacob Y. Shin ◽  
Kathryn Osann ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Clear Cell ◽  
Cell Types ◽  
Ovarian Carcinomas ◽  
Ovarian Cancers

A Cytological and Cytochemical Study of the ‘Epithelial Body’ on the Carotid Artery of the Lizards, Trachysaurus Rugosus and Tiliqua Occipitalis

1963 ◽  
Vol s3-104 (66) ◽  
pp. 197-205
Author(s):  
D. C. ROGERS
Keyword(s):  
Epithelial Cell ◽  
Parathyroid Gland ◽  
Clear Cell ◽  
Cell Types ◽  
Chief Cell ◽  
Dark Cell ◽  
Secretory Cycle ◽  
Cytochemical Study ◽  
Cytochemical Investigation ◽  
The Subject

Epithelial body III of Tiliqua occipitalis and Trachysaurus rugosus has been the subject of a histological and cytochemical investigation with the object of making a comparison with the mammalian parathyroid gland. The cytochemical reactions of the reptilian chief cell are similar to those of the mammalian ‘pale principal’ cell. On the basis of a series of cytological and cytochemical changes a secretory cycle commencing with the chief cell and culminating in the water-clear cell is postulated. Two further cell types distinguished as the dark cell and the ‘epithelial’ cell have been described in the reptilian epithelial body. The significance of these two cell types is discussed. There is some evidence suggesting that true sinusoids invest the cellular cords. There is no evidence, with the technique used, that the gland cells are innervated. It is suggested that the general histology is comparable to that of the mammalian parathyroid gland.

Functional profiling of clear cell ovarian cancer.

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. 5035-5035
Author(s):  
Rowan Miller ◽  
Rachel Brough ◽  
Ilirjana Bajrami ◽  
Stanley B. Kaye ◽  
Christopher J. Lord ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
High Throughput Screening ◽  
Clear Cell ◽  
Integral Functional ◽  
Therapeutic Targets ◽  
Molecular Profiling ◽  
Loss Of Function ◽  
Ovarian Carcinomas ◽  
Ovarian Cancers ◽  
Platinum Based Chemotherapy

5035 Background: Clear cell ovarian cancer represents up to 15% of epithelial ovarian cancers. In comparison to other subtypes, clear cell ovarian carcinomas have a poorer prognosis and are relatively resistant to standard platinum based chemotherapy. Recently, loss of function mutations in the tumour suppressor gene ARID1A were identified in up to 50% of ovarian clear cell carcinomas. We have adopted an integral functional and molecular profiling approach as a route to identify new genetic dependencies and therapeutic targets for this disease. Methods: Clear cell ovarian cancer cell lines were functionally profiled using high throughput screening with chemical and siRNA libraries. This has been integrated with molecular profiling data generated from exome and transcriptome sequencing to aid the discovery of novel targets. Results: Using functional screens we have now identified critical gene dependencies and potential therapeutics in a series of clear cell ovarian cancer models. The comparison of functional viability profiles for models characterized by ARID1A loss of function mutations is now enabling an analysis of synthetic lethal effects that could be used to target clear cell ovarian cancers carrying these mutations. Conclusions: The work undertaken so far provides the framework for the discovery of therapeutic targets for clear cell ovarian cancer using an integrated approach. Revalidation of these preliminary results is now underway to characterize new genetic dependencies for this disease.

scholarly journals Endometriosis and ovarian cancer risk

2020 ◽  
Vol 11 (4) ◽  
Author(s):  
Javier de la Torre Fernández de Vega ◽  
Jose Luis Sánchez-Iglesias ◽  
Assumpt Perez-Benavente ◽  
Antonio Gil-Moreno ◽  
Rasheda Begum Dina ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Clear Cell ◽  
Molecular Genetic ◽  
Cell Types ◽  
Ovarian Cancer Risk ◽  
New Paradigm ◽  
Ovarian Carcinomas ◽  
Genetic Studies ◽  
Ovarian Carcinogenesis ◽  
Increased Risk

Epithelial ovarian cancer presents different histological subtypes, mainly serous, mucinous, endometriod, clear cell, mixed and undifferentiated cell. Molecular genetic studies have led to a new paradigm based on a dualistic model of ovarian carcinogenesis. There is a causal association between endometriosis and specific types of ovarian carcinomas, but the magnitude of the risk is low and endometriosis is not considered a premalignant lesion. Among the endometriosis-associated ovarian tumors adenocarcinoma is the most common (Endometrioid and clear cell) (70%), sarcoma is the second most common malignancy (12%) and rare cell types 6%. The gynecologist should pay special attention to identify patients with endometriosis who may be at an increased risk for ovarian cancer.

scholarly journals Differentially Methylated Loci Distinguish Ovarian Carcinoma Histological Types: Evaluation of a DNA Methylation Assay in FFPE Tissue

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Linda E. Kelemen ◽  
Martin Köbel ◽  
Angela Chan ◽  
Soreh Taghaddos ◽  
Irina Dinu
Keyword(s):  
Clear Cell ◽  
Methylation Status ◽  
Cell Types ◽  
Dna Amount ◽  
Ovarian Carcinomas ◽  
Histological Types ◽  
Tumor Subtypes ◽  
Methylation Assay ◽  
Ffpe Tumor ◽  
Histologic Types

Epigenomic markers can identify tumor subtypes, but few platforms can accommodate formalin-fixed paraffin-embedded (FFPE) tumor tissue. We tested different amounts of bisulfite-converted (bs) DNA from six FFPE ovarian carcinomas (OC) of serous, endometrioid, and clear cell histologies and two HapMap constitutional genomes to evaluate the performance of the GoldenGate methylation assay. Methylation status at each 1,505 CpG site was expressed asβ-values. Comparing 400 ng versus 250 ng bsDNA, reproducibility of the assay ranged from Spearmanr2=0.41to 0.90, indicating thatβ-values obtained with a lower DNA amount did not always correlate well with the higher amount. Average methylation for the six samples was higher using 250 ng (β-value = 0.45,SD=0.29) than with 400 ng (β-value = 0.36,SD=0.32). Reproducibility between duplicate HapMap samples (r2=0.76to 0.92) was also variable. Using 400 ng input bsDNA,THBS2andERGwere differentially methylated across all histologic types and between endometrioid and clear cell types at <0.1% false discovery rate. Methylation did not always correlate with gene expression (r2=-0.70to 0.15). We found that lower bsDNA overestimates methylation, and, using higher bsDNA amounts, we confirmed a previous report of higher methylation ofTHBS2in clear cell OC, which could provide new insight into biological pathways that distinguish OC histological types.

scholarly journals Gene Expression Patterns in Ovarian Carcinomas

2003 ◽  
Vol 14 (11) ◽  
pp. 4376-4386 ◽  
Author(s):  
Marci E. Schaner ◽  
Douglas T. Ross ◽  
Giuseppe Ciaravino ◽  
Therese Sørlie ◽  
Olga Troyanskaya ◽  
...  
Keyword(s):  
Gene Expression ◽  
Estrogen Receptor ◽  
Dna Microarrays ◽  
Clear Cell ◽  
Expression Patterns ◽  
Gene Expression Patterns ◽  
Published Data ◽  
Breast Cancers ◽  
Ovarian Carcinomas ◽  
Ovarian Cancers

We used DNA microarrays to characterize the global gene expression patterns in surface epithelial cancers of the ovary. We identified groups of genes that distinguished the clear cell subtype from other ovarian carcinomas, grade I and II from grade III serous papillary carcinomas, and ovarian from breast carcinomas. Six clear cell carcinomas were distinguished from 36 other ovarian carcinomas (predominantly serous papillary) based on their gene expression patterns. The differences may yield insights into the worse prognosis and therapeutic resistance associated with clear cell carcinomas. A comparison of the gene expression patterns in the ovarian cancers to published data of gene expression in breast cancers revealed a large number of differentially expressed genes. We identified a group of 62 genes that correctly classified all 125 breast and ovarian cancer specimens. Among the best discriminators more highly expressed in the ovarian carcinomas were PAX8 (paired box gene 8), mesothelin, and ephrin-B1 (EFNB1). Although estrogen receptor was expressed in both the ovarian and breast cancers, genes that are coregulated with the estrogen receptor in breast cancers, including GATA-3, LIV-1, and X-box binding protein 1, did not show a similar pattern of coexpression in the ovarian cancers.

Ca++-ATPase activity in the hepatopancreas cells of Oniscus asellus

1992 ◽  
Vol 50 (1) ◽  
pp. 820-821
Author(s):  
G.M. Vernon ◽  
A. Surace ◽  
R. Witkus
Keyword(s):  
B Cells ◽  
Epithelial Cell ◽  
Atpase Activity ◽  
Calcium Transport ◽  
Carcinus Maenas ◽  
Cell Types ◽  
Molt Cycle ◽  
Copper And Zinc

The hepatopancreas consists of a pair of bilobed tubules comprised of two epithelial cell types. S cells are absorptive and accumulate metals such as copper and zinc. Ca++ concentrations vary between the S and B cells and during the molt cycle. Roer and Dillaman implicated Ca++-ATPase in calcium transport during molting in Carcinus maenas. This study was undertaken to compare the localization of Ca++-ATPase activity in the S and B cells during intermolt.

Molecular stratification of clear cell ovarian carcinomas

2020 ◽  
Author(s):  
L Feil ◽  
J Senz ◽  
M Ta ◽  
J Huvila ◽  
K Greif ◽  
...  
Keyword(s):  
Clear Cell ◽  
Ovarian Carcinomas ◽  
Molecular Stratification

Pathogenic Mechanisms Underlying Idiopathic Pulmonary Fibrosis

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Benjamin J. Moss ◽  
Stefan W. Ryter ◽  
Ivan O. Rosas
Keyword(s):  
Epithelial Cell ◽  
Idiopathic Pulmonary Fibrosis ◽  
Pulmonary Fibrosis ◽  
Cell Activation ◽  
Alveolar Epithelial Cell ◽  
Cell Types ◽  
Annual Review ◽  
Publication Date ◽  
Metabolic Dysfunction ◽  
Epithelial Repair

The pathogenesis of idiopathic pulmonary fibrosis (IPF) involves a complex interplay of cell types and signaling pathways. Recurrent alveolar epithelial cell (AEC) injury may occur in the context of predisposing factors (e.g., genetic, environmental, epigenetic, immunologic, and gerontologic), leading to metabolic dysfunction, senescence, aberrant epithelial cell activation, and dysregulated epithelial repair. The dysregulated epithelial cell interacts with mesenchymal, immune, and endothelial cells via multiple signaling mechanisms to trigger fibroblast and myofibroblast activation. Recent single-cell RNA sequencing studies of IPF lungs support the epithelial injury model. These studies have uncovered a novel type of AEC with characteristics of an aberrant basal cell, which may disrupt normal epithelial repair and propagate a profibrotic phenotype. Here, we review the pathogenesis of IPF in the context of novel bioinformatics tools as strategies to discover pathways of disease, cell-specific mechanisms, and cell-cell interactions that propagate the profibrotic niche. Expected final online publication date for the Annual Review of Pathology: Mechanisms of Disease, Volume 17 is January 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

Deficiency in intercellular communication in two established renal epithelial cell lines (LLC-PK1 and MDCK)

1984 ◽  
Vol 66 (1) ◽  
pp. 81-93
Author(s):  
F.V. Sepulveda ◽  
J.D. Pearson
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Mdck Cells ◽  
Cell Communication ◽  
Intercellular Junctions ◽  
Cell Types ◽  
Renal Epithelial Cell ◽  
Aortic Endothelial Cells ◽  
Epithelial Cell Lines ◽  
Autoradiographic Analysis

We have studied the cell-to-cell passage of uridine nucleotides in two renal epithelial cell lines (LLC-PK1 and MDCK) and in porcine aortic endothelial cells (PAE). All three cell types incorporated tritiated uridine. After a 3 h incubation the radioactivity was predominantly in the form of acid-soluble compounds, mainly UTP. Prelabelled LLC-PK1 or MDCK cells were unable to transfer radioactivity to added adjacent, non-labelled cells, whereas PAE cells readily formed communicating intercellular junctions, as judged by autoradiographic analysis after a 3 h co-culture period. Cell-to-cell communication in either of the renal cell lines was not promoted by treatment with dibutyryl cyclic AMP and methylisobutylxanthine. Radioactivity incorporated into the acid-insoluble pool was not available for intercellular transfer, as assessed in experiments in which cells were prelabelled 24 h before co-culture.

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