scholarly journals Disulfiram Chelated with Copper Promotes Apoptosis in Osteosarcoma via ROS/Mitochondria Pathway

2021 ◽  
Vol 44 (10) ◽  
pp. 1557-1564
Author(s):  
Yelong Ren ◽  
Yutian Lin ◽  
Jinghao Chen ◽  
Yonglong Jin
Keyword(s):  
Mitochondria Pathway

The Effect and Mechanism of Apoptosis Induced by Desacetylcinobufotalin (DEBF) in Human Hepatocellular Carcinoma HepG2 Cells

2013 ◽  
Vol 395-396 ◽  
pp. 587-590
Author(s):  
Xu Chao ◽  
Lin Dang ◽  
Min Hui Wei
Keyword(s):  
Hepatocellular Carcinoma ◽  
Antitumor Activity ◽  
Western Blot ◽  
Hepg2 Cells ◽  
Human Hepatocellular Carcinoma ◽  
Inhibition Effect ◽  
Marked Inhibition ◽  
Mitochondria Pathway

The cytotoxicity of Desacetylcinobufotalin (DEBF) and apoptosis induced by DEBF was measured. Additionally the mechanism of Apoptosis induced by DEBF was studied through Western blot. The results show DEBF displayed the marked inhibition effect to HepG2 cells and the IC50value is 0.0279μmol/ml. The expression of Bax was significantly increased and the expression of Bcl-2 was markedly decreased, compared to the control. The data suggest DEBF had significant antitumor activity through induction apoptosis via mitochondria pathway.

Aquatic birnavirus capsid protein, VP3, induces apoptosis via the Bad-mediated mitochondria pathway in fish and mouse cells

APOPTOSIS ◽  
2010 ◽  
Vol 15 (6) ◽  
pp. 653-668 ◽  
Author(s):  
Chien-Li Chiu ◽  
Jen-Leih Wu ◽  
Guor-Mour Her ◽  
Yi-Li Chou ◽  
Jiann-Ruey Hong
Keyword(s):  
Capsid Protein ◽  
Mouse Cells ◽  
Mitochondria Pathway

The protective effects of selenium on cadmium-induced oxidative stress and apoptosis via mitochondria pathway in mice kidney

2013 ◽  
Vol 58 ◽  
pp. 61-67 ◽  
Author(s):  
Yang Wang ◽  
Yuchen Wu ◽  
Kang Luo ◽  
Yuxin Liu ◽  
Ming Zhou ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Protective Effects ◽  
Mitochondria Pathway

9-Methoxycamptothecin from Nothapodytes foetida Induces Apoptosis in Murine Sarcoma S180 Cells

2011 ◽  
Vol 66 (9-10) ◽  
pp. 471-476
Author(s):  
Na Liao ◽  
Peng Zhang ◽  
Mingzhang Ao ◽  
Jing Wang ◽  
Yueyuan Shi ◽  
...  
Keyword(s):  
Cell Death ◽  
Nmr Spectroscopy ◽  
Mtt Assay ◽  
Propidium Iodide ◽  
Annexin V ◽  
Double Staining ◽  
Nothapodytes Foetida ◽  
Ic50 Value ◽  
Mitochondria Pathway ◽  
Murine Sarcoma

9-Methoxycamptothecin (MCPT) was found to have antitumour activities through topoisomerase inhibition. However, the type of cell death induced in the tumour cells treated with MCPT was not elucidated. In this study, MCPT and camptothecin were isolated from Nothapodytes foetida distributed in Hubei Province, China and identifi ed by NMR spectroscopy. MCPT was tested by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay using camptothecin as reference. Annexin V-FITC/propidium iodide double staining and real-time PCR were also performed. The IC50 value was (0.385 ± 0.08) μM. The apoptosis rates increased from 9.5% to 17.27%, 30.14%, and 66.46% with an increase in MCPT concentrations from 0, 0.19, 0.38, to 0.95 μM, respectively. The ratio of Bax/Bcl-2 also increased from 1 to 1.61, 2.43, and 4.57, respectively. Bax and Bcl-2 are crucial to the mitochondria pathway. The results indicate that the mitochondria pathway may be involed in MCPT-induced murine sarcoma S180 apoptosis.

Increase of Sensitivity to Chemotherapy and NK-Mediated Cytotoxicity of Leukemic Stem Cell by a Farnesyltransferase Inhibitor.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 4161-4161
Author(s):  
Miaorong She ◽  
Jinggao Li ◽  
Xilin Chen ◽  
Xingqing Niu ◽  
Kunyuan Guo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Malignant Disease ◽  
Nk Cell ◽  
Conflicts Of Interest ◽  
Nkg2d Ligands ◽  
Enhanced Sensitivity ◽  
Cell Immunotherapy ◽  
Bcl2 Protein ◽  
Mitochondria Pathway ◽  
Induced Apoptosis

Abstract Abstract 4161 Acute myeloid leukemia (AML) is a serious and often lethal hematopoietic malignant disease arising from stem cells. Leukemic stem cells (LSCs) play the central role in the relapse and refractory of AML and highlight the critical need for the new therapeutic strategies to directly target the LSC population. We previous found that LSC were resistant to chemotherapy and NK-mediated cytotoxicity, which were resulted from the apoptosis defect and NKG2D ligands change. Our previous data also showed that Manumycin induced apoptosis in leukemia cells through mitochondria pathway. Here, we demonstrated that manumycin increased the sensitivity of LSC to chemotherapy and NK cell-mediated cytotoxicity. Manumycin enhanced sensitivity to chemotherapy in LSCs via induced apoptosis. Analysis of signaling pathways revealed that manumycin enhanced mitoxantrone activation of caspase 3 and significantly decreasing Bcl2 protein. These finding indicated that manumycin sensitized mitoxantrone to chemotherapy via down-regulating Bcl2. Importantly, manumycin enhanced the sensitivities of LSCs to NK cells cytotoxicity through up-regulating the expressions of NKG2D ligands MICA/B and ULBP3. Thus, Manumycin present a promising novel therapeutic approach for AML therapy especially when resistant to chemotherapy and NK cell immunotherapy. Disclosures: No relevant conflicts of interest to declare.

scholarly journals The Effects of ATO on Mitochondria Apoptosis Pathway Genes Expression in APL Cell Line

2016 ◽  
Vol 1 (2) ◽  
pp. 37-42
Author(s):  
Maede Sharifizadeh ◽  
Kimia Davarian ◽  
Mehrdad Hashemi ◽  
Majid Momeni ◽  
Kamran Ali Moghdam ◽  
...  
Keyword(s):  
Cell Line ◽  
Arsenic Trioxide ◽  
Caspase 3 ◽  
Single Agent ◽  
Cdna Synthesis ◽  
Apoptosis Pathway ◽  
Genes Expression ◽  
Analytical Work ◽  
Mitochondria Pathway ◽  
Mitochondria Apoptosis Pathway

Purpose and Background: Acute promyelocystic leukemia is the most malignant acute leukemia that leads to death in few weeks. It constitutes 10-15% of acute myelocystic leukemia. Arsenic trioxide, as a single agent factor, is known as the best treatment for acute promyelocystic leukemia, which mainly functions by inducing apoptosis. However, there is no clear image of the mechanism through which apoptosis is induced and how the genes expression is deeply affected. Thus, the present study is an attempt to examine the effect of the agent on expression of the genes dealing with the cancer. Methodology: The study was carried out as an analytical work. To find out about the mechanisms effective on inducing apoptosis, cell line NB4 were cultured with 0.5µM, 1 µM, and 2 µM arsenic trioxide and their RNA was extracted after 12hrs, 24hrs, 28hrs, and 72hrs. Following cDNA synthesis, apoptosis genes expression at mitochondria pathway including caspase 3, Mcl-1, and Bcl-2 were examined using Real-Time PCR. The data was analyzed using t-test and variance analysis in Excel.Findings: It was found that arsenic caused apoptosis was featured with decrease of mRNA expression of Bcl-2 anti-apoptotic. However, expression of caspase 3 and Mcl-1 genes remained unchanged after culturing by arsenic. Conclusion: The results showed that changes in Bcl-2 gene expression can be considered as a mechanism of apoptosis caused by arsenic, while caspase 3 and Mcl-1 genes had no effect on the mechanism.

scholarly journals Apoptosis of Platelets Inhibited By Herba Sarcandrae Extract through the Mitochondria Pathway

2018 ◽  
Vol 2018 ◽  
pp. 1-12
Author(s):  
Xiaoqin Zhu ◽  
Yiling Jiang ◽  
Qin Zheng ◽  
Aiping Zhang ◽  
Ling Shi ◽  
...  
Keyword(s):  
Bone Marrow Failure ◽  
Underlying Mechanism ◽  
Mitochondrial Pathway ◽  
Total Flavonoids ◽  
Suspension Cells ◽  
Caspase 9 ◽  
Immune Mediated ◽  
Mitochondria Pathway

The purpose of the present study is to decode the underlying mechanism of Herba Sarcandrae that indicated antipurpuric effect and to unveil one of its primary components, flavonoids, which play an important role. An immune mediated bone marrow failure (BMF) model in mouse was established by infusion thymus suspension cells after radiation in vivo. Platelets isolated in vitro were prepared from normal mice and BMF mice, respectively. The expressions of PS, P-selectin, PAC-1, Bax, Bad, Bid, and caspase-9 were examined by flow cytometry, and alteration of morphology of platelets under different conditions was observed. Our results indicated that the number of platelets was increased by addition of total flavonoids, and some of apoptotic markers such as Bax, Bad, Bid, and Caspase-9 were downregulated. In addition, the phosphatidylserine (PS) exposure on platelets was inhibited by total flavonoids, and the expressions of PAC-1 and P-selectin were decreased. In conclusion, it is suggested that the total flavonoids of Herba Sarcandrae may inhibit the excessive platelet apoptosis through mitochondrial pathway. In addition, activation of platelets may be also involved in mediating apoptosis of platelets.

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