Anti-inflammatory effect of diclofenac-sodium ointment (cream) in topical application.

The present study was designed to compare the anti-inflammatory effect of sodium hyaluronate, which is similar to the lubricant fluid that found naturally in the capsule of the healthy joint with diclofenac sodium, a member of NSAIDs commonly used in treatment of Osteoarthritis (OA), separately and in combination on an experimental model of osteoarthritis in rats induced by monosodium-iodoacetate (MIA). Twenty-five male albino rats weighing at the beginning of the experiment 160± 20 gm were used in this study. Rats were housed in cages at 25± 0.5°C. The rats were divided into 5 main groups.

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Pharmacological and Biochemical evaluation of anti-arthritic activity of Justicia gendarussa extract in FCA induced arthritis in Wistar rats

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v12i3.4769 ◽

2021 ◽

Vol 12 (3) ◽

pp. 1699-1708

Author(s):

Ashish ◽

Anjali ◽

Praveen K Dixit ◽

Nagarajan K

Keyword(s):

Body Weight ◽

Protein Denaturation ◽

Hematological Parameters ◽

Diclofenac Sodium ◽

Paw Edema ◽

Arthritis Model ◽

Inflammatory Models ◽

Anti Inflammatory ◽

Inflammatory Effect

The present study is carried out to evaluate anti-inflammatory and anti-arthritic potential of Justicia gendarussa. Leaves and stem extracted with ethanol and chloroform. These extract is tested against in-vitro (HRBC membrane stabilization method and Inhibition of protein denaturation method) and in-vivo (Carrageenan induced paw edema model) anti-inflammatory models. To assess the anti-arthritic activity, FCA induced arthritis model is used. Assessment of arthritis is done by paw volume, joint diameter, body weight, biochemical parameters, hematological parameters (Hb, RBC, WBC, ESR). The effect of in-vitro anti-inflammatory model depends on concentration. Both test extract and standard Diclofenac sodium has been shown concentration dependent effect. The maximum anti-inflammatory effect of the test extract achieved at 2000μg/ml. The test (JGLE, JGLC, JGSE) has been shown inhibition of paw edema induced by carrageenan at 50mg/kg body weight. The extract JGLE, JGLC, JGSE at 50mg/kg body weight and 100mg/kg body weight orally showed the significant (P< 0.05) and dose dependent inhibitory effect against FCA induced arthritis model. Diclofenac sodium 20mg/kg body weight orally is used as a standard. JGLE exhibit more significant and most promising anti-arthritic and anti-inflammatory effect than other extracts these effects support the traditional role of J. gendarussa in arthritis and other inflammatory condition.

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ANTI INFLAMMATORY EFFECT OF CIPROFLOXACIN, AZITHROMYCIN AND DICLOFENAC SODIUM ON CARRAGEENAN INDUCED HIND PAW EDEMA IN MICE

International Journal of Clinical and Biomedical Research ◽

10.5455/ijcbr.2017.34.11 ◽

2017 ◽

Vol 3 (4) ◽

pp. 50

Author(s):

Rukma E ◽

Sridhar I ◽

Srikanth B

Keyword(s):

Diclofenac Sodium ◽

Paw Edema ◽

Anti Inflammatory ◽

Inflammatory Effect

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ANTIINFLAMMATION ACTIVITY ETHANOL EXTRACT OF MARBOSI-BOSI LEAVES (Tarenna polycarpa (Miq.) Koord.Ex Valeton) ON WHITE RAT IN CARRAGEENAN INDUCED PAW INFLAMMATION

Asian Journal of Pharmaceutical Research and Development ◽

10.22270/ajprd.v6i3.376 ◽

2018 ◽

Vol 6 (3) ◽

pp. 1-4

Author(s):

Haris Munandarnst ◽

Marline N

Keyword(s):

Diclofenac Sodium ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Inflammatory Activity ◽

Group Iii ◽

Rat Paw Edema ◽

Group I ◽

Anti Inflammatory ◽

Inflammatory Effect

Traditionally, (Tarenna polycarpa (Miq.) Koord Ex Valeton as known as marbosi-bosi which commonly found in Sibolga, North Sumatera, Indonesia has been used as antidiabetes, cholesterol, anti-inflammatory and antibacterial. Tarenna species has been found its activities as antimicrobial, anti-oxidant and anti-inflammatory activity. The aim of this study was to investigate the anti inflammatory effect ethanol extract of marbosi-bosi leaves (Tarenna polycarpa (Miq.) Koord Ex Valeton in terms of decreased edema volume of male white rat in 1% carrageenan-induced and also to determine the effective dose of extract to decrease the volume of rat paw edema Ethanol extract of marbosi-bosi (Tarenna polycarpa (Miq.) Koord Ex Valeton was obtained by maceration. The antiinflammatory activity test was divided into 5 groups. The Group I (negative control) was given CMC 0.5%, Group II (positive control) was given diclofenac sodium 2,25 mg / kg BW, while Group III, IV and V were given marbosi-bosi leaf extract at a dose of 50, 100 and 200 mg/kgBW respectively. Each rat was induced by 1% carrageenan subplantar injection. Examination of antiinflammatory effect was measured by using digital plethysmometer at minute of 30 to minute of 360. The data were analyzed statistically using ANOVA (analysis of variance).The results showed that negative control did not show anti-inflammatory effect had significant differences with other treatment groups. In conclusion, ethanol extract marbosi-bosi (Tarenna polycarpa (Miq.) Koord Ex Valeton has an effective anti-inflammatory activity at a dose of 100 mg / kgBW.

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Effectivity of Holothuria scabra and Spirulina platensis extract combination as an Antiinflammatory Agent Measured by Carrageenan-induced Rat Paw Edema

ILMU KELAUTAN Indonesian Journal of Marine Sciences ◽

10.14710/ik.ijms.25.3.103-109 ◽

2020 ◽

Vol 25 (3) ◽

pp. 103-109

Author(s):

Renni Yuniati ◽

Bambang Sulardiono

Keyword(s):

Spirulina Platensis ◽

Diclofenac Sodium ◽

Positive Control ◽

Control Group ◽

Paw Edema ◽

Holothuria Scabra ◽

Rat Paw Edema ◽

Anti Inflammatory ◽

Edema Inhibition ◽

Inflammatory Effect

Sea cucumber, Holothuria scabra, can be found abundantly in Indonesian seas, which is also known to possess several medicinal properties. Spirulina platensis is another marine resources that has recently been extensively researched for its medicinal ability, such as anti-inflammatory effect. This study aims to evaluate the efficacy of H. scabra extract combined with S. platensis extract in reducing inflammation. This study uses male Wistar rats as the study animal. Inflammation was induced by injecting carrageenan solution into the mice paw. Combination of H. scabra and S. platensis extract with various combination ratio (1:1; 1:2; and 2:1) was applied to the mice paw. Diclofenac sodium was used as the standard control therapy. Edema inhibition rate and anti-inflammatory efficacy were measured by analyzing the edema size and calculating the edema difference. Combination of H. scabra and S. platensis with 1:1 ratio has the largest edema inhibition volume compared to the other treatments. H. scabra and S. platensis combination outperforms the positive diclofenac sodium control group in terms of edema inhibition. The highest anti-inflammatory effect is obtained in the combination of H. scabra and S. platensis with 1:2 ratio, however, the anti-inflammatory efficacy is not as potent as the positive control. The effectivity of Holothuria scabra and Spirulina platensis extract in reducing the edema might be caused by their ability to reduce the levels of several inflammatory markers, including IL-6, NO, MMP9, and COX-2. This result suggests that H. scabra and S. platensis combination has anti-inflammatory effect shown in mice paw edema model.

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ANTI-INFLAMMATORY EFFECT OF ZINGIBER OFFICINALE ON SPRAGUE DAWLEY RATS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i3.16251 ◽

2017 ◽

Vol 10 (3) ◽

pp. 353 ◽

Cited By ~ 1

Author(s):

Rohini Karunakaran ◽

Ndanusa Abdullahi Hassan ◽

Uma Sankar A ◽

Khin Mar Aye

Keyword(s):

Aqueous Extract ◽

Diclofenac Sodium ◽

Zingiber Officinale ◽

Control Group ◽

Sprague Dawley ◽

Reference Drug ◽

Sd Rats ◽

Sprague Dawley Rats ◽

Anti Inflammatory ◽

Inflammatory Effect

ABSTRACTObjective: To investigate the anti-inflammatory effect of aqueous extract of Zingiber officinale on carrageenan-induced inflammation on SpragueDawley (SD) rats.Methods: SD rats were divided into six of five groups and allowed to acclimatize for 1 week. Inflammation was induced on the animal by injecting theright hand paw with carrageenan (0.1 ml of 1%). Group 1 was given normal saline and served as a control. Group 2 was fed with food and water andserved as the carrageenan control. Group 3 was given 200 mg/kg aqueous extract of ginger, Group 4 with 400 mg/kg aqueous extract of ginger, andGroup 5 with 150 mg/kg diclofenac sodium (reference drug for inflammation).Results: The paw edema in carrageenan-induced SD rats was considerably reduced by treating with 400 mg/kg aqueous ginger extracts whencompared to the untreated SD rats (p<0.001).Conclusion: This study indicates that aqueous extract of Z. officinale possesses anti-inflammatory properties.Keywords: Anti-inflammatory, Sprague Dawley rats, Zingiber officinale, Carrageenan, Edema

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Changes in Phospholipid/Ceramide Profiles and Eicosanoid Levels in the Plasma of Rats Irradiated with UV Rays and Treated Topically with Cannabidiol

International Journal of Molecular Sciences ◽

10.3390/ijms22168700 ◽

2021 ◽

Vol 22 (16) ◽

pp. 8700

Author(s):

Wojciech Łuczaj ◽

Anna Jastrząb ◽

Maria do Rosário Domingues ◽

Pedro Domingues ◽

Elżbieta Skrzydlewska

Keyword(s):

Oxidative Stress ◽

Antioxidant Capacity ◽

Uv Radiation ◽

Topical Application ◽

Blood Cells ◽

Plasma Phospholipid ◽

Uvb Radiation ◽

Anti Inflammatory ◽

Phospholipid Profile ◽

Inflammatory Effect

Chronic UV radiation causes oxidative stress and inflammation of skin and blood cells. Therefore, in this study, we assessed the effects of cannabidiol (CBD), a natural phytocannabinoid with antioxidant and anti-inflammatory properties, on the phospholipid (PL) and ceramide (CER) profiles in the plasma of nude rats irradiated with UVA/UVB and treated topically with CBD. The results obtained showed that UVA/UVB radiation increased the levels of phosphatidylcholines, lysophospholipids, and eicosanoids (PGE2, TxB2), while downregulation of sphingomyelins led to an increase in CER[NS] and CER[NDS]. Topical application of CBD to the skin of control rats significantly upregulated plasma ether-linked phosphatidylethanolamines (PEo) and ceramides. However, CBD administered to rats irradiated with UVA/UVB promoted further upregulation of CER and PEo and led to significant downregulation of lysophospholipids. This was accompanied by the anti-inflammatory effect of CBD, manifested by a reduction in the levels of proinflammatory PGE2 and TxB2 and a dramatic increase in the level of anti-inflammatory LPXA4. It can therefore be suggested that topical application of CBD to the skin of rats exposed to UVA/UVB radiation prevents changes in plasma phospholipid profile resulting in a reduction of inflammation by reducing the level of LPE and LPC species and increasing antioxidant capacity due to upregulation of PEo species.

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