scholarly journals Infection and pathological lesions of lymph nodes induced by Linguatula serrata nymphs in one-humped camels in Iran

2018 ◽  
Vol 68 (4) ◽  
pp. 529 ◽  
Author(s):  
M. TAVASSOLI ◽  
R. HOBBENAGHI ◽  
A. KARGOZARI ◽  
H. REZAEIA
Keyword(s):  
Lymph Nodes ◽  
Giant Cells ◽  
Intermediate Hosts ◽  
Mesenteric Lymph Nodes ◽  
Granulomatous Reaction ◽  
Linguatula Serrata ◽  
Mesenteric Lymph ◽  
Zoonotic Parasite ◽  
Macroscopic Examination ◽  
Very High

Linguatula serrata (Pentastomida: Linguatulidae) known tong-worm is a cosmopolitan, zoonotic, and obligate endoparasite. The parasite lives in the nasopharyngeal region of the final hosts, which primarily include dogs and other carnivores. Various herbivores, including, camels serve as the best intermediate hosts for nymph stages. In present study the mesenteric lymph nodes of 101camels were examined for infection to L. serrata macroscopically and histopathologically. The infected and normal lymph nodes were processed for histopathology. The results indicated that out of 101 sampled 33 (32.67%) were infected. Macroscopic examination revealed that the infected lymph nodes are swollen and dark, with rubbery consistency, some with subcapsular hemorrhage on cutting. Extensive hemorrhage occurred in various parts of infected lymph nodes. A section of L. serrata parasite was observed near one of the hemorrhage centers. Neutrophil count was very high in these centers and giant cells were present around the parasite, indicating granulomatous reaction. Our findings confirmed that different regions of Iran is an endemic for L. serrata infections. Because L. serrata is a zoonotic parasite, preventive measures should be adopted to disrupt the parasite’s life cycle and minimize the risk of infection in both humans and other animals.

Histopathological changes caused by the nymph stage of Linguatula serrata in the mesenteric lymph nodes of goats

2013 ◽  
Vol 61 (1) ◽  
pp. 36-41 ◽  
Author(s):  
Mohammad Yakhchali ◽  
Ali Tehrani
Keyword(s):  
Lymph Nodes ◽  
Human Infection ◽  
Histopathological Changes ◽  
Intermediate Hosts ◽  
Mesenteric Lymph Nodes ◽  
Linguatula Serrata ◽  
Vascular Abnormality ◽  
Nymphal Stage ◽  
Mesenteric Lymph ◽  
Main Factor

Linguatula serrata (Fröhlich, 1789) is a ubiquitous parasite species belonging to pentastomids, which has been reported frequently from domestic herbivores of Iran. This study was carried out to elucidate the pathological changes caused by the nymphal stage of L. serrata in the mesenteric lymph nodes (MLNs) of goats. Jejunal and ileal MLNs of slaughtered goats infected with L. serrata were collected and fixed in 10% neutral formal saline. Histopathological changes included follicular necrosis accompanied by a loss of lymphocytes and oedematous cystlike spaces. Granulomatous lesions were formed by mononuclear cell infiltration and necrotic tissue. Some lymphocytes were apoptotic with chromatolysis. A more frequent vascular abnormality was vasculitis. These findings show that larval migration is the main factor to initiate trauma and cause irritation in the MLNs of goats. Since goats are habitual intermediate hosts in Iran, rigorous inspection of the MLNs of slaughtered goats is recommended in order to decrease the chance of human infection through the consumption of raw visceral organs of goats.

scholarly journals Epizootic Disseminated Toxoplasmosis in Captive Slender-tailed Meerkats (Suricata suricatta)

1997 ◽  
Vol 34 (1) ◽  
pp. 1-7 ◽  
Author(s):  
C. Juan-Sallés ◽  
N. Prats ◽  
S. López ◽  
M. Domingo ◽  
A. J. Marco ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Neospora Caninum ◽  
Clinical Signs ◽  
Giant Cells ◽  
Mesenteric Lymph Nodes ◽  
Suricata Suricatta ◽  
Mesenteric Lymph ◽  
Clinicopathologic Findings ◽  
Foamy Macrophages

Fatal disseminated toxoplasmosis was diagnosed in seven captive slender-tailed meerkats ( Suricata suricatta) according to clinicopathologic findings and immunohistochemistry. Five of nine meerkats died during an outbreak in late 1994. These included four kits (2.5 to 4.5 months old) and a 4-year-old meerkat. Two other meerkats, both adults, died in 1992 and 1995. Respiratory insufficiency (4/7) and incoordination (3/7) were the most consistent clinical signs, although two of seven meerkats died unexpectedly. At necropsy, the lungs were reddened and noncollapsed (6/7), and had multiple pale round foci (4/7). Yellow foci of necrosis in mesenteric lymph nodes (4/7), splenomegaly (3/7), and hydropericardium (3/7) were other common gross findings. Microscopically, interstitial pneumonia was present in all seven meerkats, being acute to subacute in six of them. Type 2 pneumocyte hyperplasia, aggregates of foamy macrophages, and giant cells were consistently seen. Multifocal to locally extensive necrosis of mesenteric lymph nodes (4/7), mild to severe multifocal necrotizing hepatitis (5/6), and mild nonsuppurative encephalitis (4/6) were also seen. Toxoplasma-like organisms were consistently associated with these lesions and were stained by the avidin biotin peroxidase procedure with an antiserum that does not cross-react with Neospora caninum. Meerkats were most likely infected after an oral, primary exposure to Toxoplasma. Several observations indicate that meerkats may be highly susceptible to toxoplasmosis.

scholarly journals Prevalence of Linguatula serrata nymphs in slaughtered sheep from Tehran province, Iran

2019 ◽  
Vol 22 (4) ◽  
pp. 487-492
Author(s):  
S. Bokaie ◽  
A. Khanjari ◽  
M.-H. Rabiee ◽  
B. Hajimohammadi ◽  
S. Shirali ◽  
...  
Keyword(s):  
Infection Rate ◽  
Random Sampling ◽  
Preventive Measures ◽  
Intermediate Hosts ◽  
Mesenteric Lymph Nodes ◽  
Linguatula Serrata ◽  
Systematic Random Sampling ◽  
Mesenteric Lymph ◽  
Important Intermediate ◽  
Tehran Province

Linguatulosis is a zoonotic disease caused by Linguatula serrata. Ruminants are the most important intermediate hosts of this parasite. This study was carried out to evaluate the prevalence rate of Linguatula serrata infection in slaughtered sheep in slaughterhouses of Tehran province, Iran. A total of 767 slaughtered sheep were selected in 2016 using systematic random sampling and their mesenteric lymph nodes were examined for Linguatula serrata nymph detection. Then, the infection rate and its intensity in different ages, sex and seasons were assessed. It was revealed that 66 sheep (8.6%) were infected with Linguatula serrata nymphs. Intensity of the infection was 4.88 nymphs. The highest rate of infection was observed in sheep aged over 19 months (P≤0.05). The highest infection rate and intensity of the infection were recorded in winter and summer, respectively (P≤0.05). Besides, intensity of the infection in females was significantly higher than that in males (P≤0.05). This study indicates the importance of Linguatula serrata infection in sheep in Tehran province, and the need to improve preventive measures for reducing the rate of infection and preventing transmission of the infection from sheep and other ruminant to other animals and humans in Iran.

Ultrastructural studies of bovine paratuberculosis (Johne's Disease)

2015 ◽  
Vol 15 (2) ◽  
pp. 61-65
Author(s):  
T Batbayar ◽  
S Andrei ◽  
Ch Tungalag
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Avium ◽  
Giant Cells ◽  
Mycobacterium Avium Subsp ◽  
Epithelioid Cells ◽  
Mesenteric Lymph Nodes ◽  
Ultrastructural Studies ◽  
Mesenteric Lymph ◽  
Agricultural Sciences ◽  
Mycobacterium Avium Subsp Paratuberculosis

In the present study, we report the ultrastructural morphologic features of infected macrophages, epithelioid cells, Langhans' giant cells and a more detailed impression of the interaction between macrophages and engulfed bacteria in ileum and mesenteric lymph nodes of cows naturally infected with Mycobacterium avium subsp. paratuberculosis (MAP).Mongolian Journal of Agricultural Sciences Vol.15(2) 2015; 61-65 

scholarly journals Peritoneal Tuberculosis in an Immunocompetent, Unknown Risk Patient

2013 ◽  
Vol 2013 ◽  
pp. 1-3
Author(s):  
Yutaka Tomizawa ◽  
Emmanuelle B. Yecies ◽  
Fiona E. Craig ◽  
Adam Sohnen
Keyword(s):  
Lymph Nodes ◽  
Oral Intake ◽  
Excisional Biopsy ◽  
Giant Cells ◽  
Peritoneal Tuberculosis ◽  
Mesenteric Lymph Nodes ◽  
Significant Past Medical History ◽  
Mesenteric Lymph ◽  
Bowel Movements ◽  
Night Sweats

A 36-year-old man with no significant past medical history presented with two-month abdominal distention, night sweats, and weight loss of 15 Ib. He had no known exposure to tuberculosis. PPD test was negative prior to the hospital admission. Physical examination was notable for new onset ascites, but no superficial lymphadenopathy or stigmata of chronic liver disease was found. CT scan demonstrated enlarged mesenteric lymph nodes, and prominent retroperitoneal lymph nodes along with moderate ascites and omental infiltration. Diagnostic paracentesis yielded WBC of 295/mm3, lymphocytic predominance (70%), and serum ascitic albumin gradient of 0.1, consistent with exudate. Both the ascitic culture and AFB smear were negative, and ascitic cytology revealed nonmalignant cells. Exploratory laparoscopy for excisional biopsy of mesenteric lymph nodes was performed. Pathologic findings revealed caseous granulomas with scattered multinucleated giant cells. Mesenteric lymph node tissue culture subsequently grewMycobacterium tuberculosiscomplex and the diagnosis of peritoneal tuberculosis was confirmed. The patient was started on quadruple therapy. A couple of days after the antibiotics were started, the small bowel obstruction started to resolve with resumption of bowel movements and tolerance of oral intake. A week later, ascites stopped accumulating and fever was no longer noted. He has been well and continues to be under observation.

scholarly journals Progressive Pathological Changes in Sub-clinical Experimental Para-tuberculosis in Goats

2017 ◽  
Vol 34 ◽  
pp. 69-84
Author(s):  
U.M. Singh ◽  
B.N. Tripathi ◽  
O.P. Paliwal
Keyword(s):  
Lymph Nodes ◽  
Post Infection ◽  
Diagnostic Tests ◽  
Giant Cells ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Mesenteric Fat ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Histopathological Studies ◽  
Mucosal Thickening

An experiment was carried out to study the development of sequential histological lesions and efficacy of certain diagnostic tests in experimentally induced subclinical paratuberculosis infection in goats. Twelve goats of 8-12 weeks age were infected with 4.23 x 109 Mycobacterium avium subsp. Paratuberculosis on 8 occasions. Eight goats were kept as in-contact controls and 4 as uninfected controls. Diagnostic tests, gross and histopathological studies were carried out at 3, 6, 9 and 12 months post-infection. Two goats were positive in the AGID test at 12MPI. Three goats, one at 9 MPI and two at 12 MPI were positive in faecal culture. Tissue PCR detected one goat positive each at 9 and 12 MPI. Six (50%) of 12 infected goats had gross and histological lesions. Marked enlargements of the mesenteric lymph nodes were observed in the early stages (3 and 6 months post infection) of infection. Mucosal thickening and corrugation of the jejunal and ileal mucosae, enlargement and oedema of the mesenteric lymph nodes, dilatation of lymphatics and gelatinisation of mesenteric fat were observed in goats euthanised at 9 and 12 MPI. Histological lesions consisting of flat and broad villi with mild infiltration of lymphocytes and macrophages in the intestinal villi and crypts were common at 3 MPI and 6 MPI. Focal infiltration of macrophages in Peyer's patches and giant cells were found in the mesenteric lymph nodes at 6 MPI. Broadened villi and increased infiltration of lymphocytes and macrophages with Langhan's giant cells were observed in the villi and crypts at 9 MPI and 12 MPI goats. Acid-fast bacilli were detected in 3 goats, one each at 6, 9 and 12 MPI.

scholarly journals Effects of aspirin on mesenteric lymph nodes of rabbits as basis for its use on lymph nodes metastases

2012 ◽  
Vol 27 (11) ◽  
pp. 795-801 ◽  
Author(s):  
Rodrigo Peduti Batista ◽  
Rafael Denadai ◽  
Rogério Saad-Hossne
Keyword(s):  
Lymph Node ◽  
Foreign Body ◽  
Cancer Therapy ◽  
Lymph Nodes ◽  
Giant Cells ◽  
Germinal Centers ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Extensive Necrosis ◽  
Lymph Nodes Metastases

PURPOSE: To evaluate the effects of aspirin 10% and 20% on mesenteric lymph nodes of rabbits as basis for its use on lymph nodes metastases. METHODS: A total of 20 lymph nodes from 20 rabbits (randomized in four groups) were evaluated. Aspirin solutions 10% (groups A and C) and 20% (groups B and D) were injected into mesenteric lymph nodes of healthy rabbits and had its gross and histological effects evaluated at 24 hours (groups A and B) and at seven days (groups C and D). RESULTS: In the groups A and B evaluated at 24 hours it was observed extensive necrosis and hemorrhage, a significant increase in apoptosis throughout the lymph node with medullary sinuses enlargement and an increase in germinal centers. In the groups C and D evaluated at seven days of solution injection there was also an increase in apoptosis with higher elevation of histiocytes and a significant decrease of necrosis and an increase of giant cells was noticed causing a foreign body chronic inflammation. In all comparisons, there were no differences between the concentrations used (10 and 20%). CONCLUSIONS: The injection of aspirin on lymph nodes caused necrosis and an increase of apoptosis after 24 hours and after seven days of treatment there was regeneration of the lymph nodes, with intense decrease of necrosis and a great elevation of apoptosis. These experimental results support future clinical studies on application of aspirin in the treatment of lymphatic metastases, since the increase of apoptosis is one of the pillars of cancer therapy.

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