scholarly journals Detection and analysis of new psittacine beak and feather disease virus (PBFDv) nucleotide sequences

2018 ◽  
Vol 68 (4) ◽  
pp. 653
Author(s):  
M VUCICEVIC ◽  
I VUCICEVIC ◽  
D DAVITKOV ◽  
D DAVITKOV ◽  
J STEVANOVIC ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Disease Virus ◽  
Clinical Signs ◽  
Nucleotide Sequences ◽  
Histopathological Evaluation ◽  
Close Relationship ◽  
Pcr Products ◽  
Pcr Method ◽  
Feather Disease Virus

Psittacine beak and feather disease (PBFD) affects a large number of Psittaciformes species. In this study, five White Cockatoo parrots (Cacatua alba) with clinical signs of PBFD were examined. After euthanasia, a full necropsy of parrots was performed and organs with macroscopic changes were sampled for routine histopathological evaluation. To confirm the presence of psittacine beak and feather disease virus (PBFDv), feather samples were analyzed with the PCR method. Sequence analysis of the obtained PCR products indicated their close relationship (99%) to other PBFDv isolates. Six variable nucleotide sites were discovered, two missense and four silent mutations. This paper presents the evidence of new PBFDv sequence in Cockatoo species.

scholarly journals Beak and feather disease virus haemagglutinating activity using erythrocytes from African Grey parrots and Brown-headed parrots : research communication

2005 ◽  
Vol 72 (3) ◽  
Author(s):  
K. Kondiah ◽  
J. Albertyn ◽  
R.R. Bragg
Keyword(s):  
South Africa ◽  
Disease Virus ◽  
Causative Agent ◽  
Haemagglutination Inhibition ◽  
Clinical Signs ◽  
Viral Disease ◽  
Dna Virus ◽  
Haemagglutinating Activity ◽  
Feather Disease Virus ◽  
Positive Results

Psittacine beak and feather disease (PBFD) is a common viral disease of wild and captive psittacine birds characterized by symmetric feather loss and beak deformities. The causative agent, beak and feather disease virus (BFDV), is a small, circular single-stranded DNA virus that belongs to the genus Circovirus. BFDV can be detected by PCR or the use of haemagglutination (HA) and haemagglutination inhibition (HI) assays that detect antigen and antibodies respectively. Erythrocytes from a limited number of psittacine species of Australian origin can be used in these tests. In South Africa, the high cost of these birds makes them difficult to obtain for experimental purposes. Investigation into the use of erythrocytes from African Grey parrots and Brown-headed parrots yielded positive results showing the haemagglutinating activity of their erythrocytes with purified BFDV obtained from confirmed clinical cases of the disease. The HA activity was further confirmed by the demonstration of HI using BFDV antiserum from three different African Grey parrots previously exposed to the virus and not showing clinical signs of the disease.

Beak and feather disease virus: correlation between viral load and clinical signs in wild Cape parrots (Poicepahlus robustus) in South Africa

2014 ◽  
Vol 160 (1) ◽  
pp. 339-344 ◽  
Author(s):  
Guy L. Regnard ◽  
Rutledge S. Boyes ◽  
Rowan O. Martin ◽  
Inga I. Hitzeroth ◽  
Edward P. Rybicki
Keyword(s):  
South Africa ◽  
Viral Load ◽  
Disease Virus ◽  
Clinical Signs ◽  
Feather Disease Virus

scholarly journals Occurrence of Aleutian mink disease in selected national mink farms in light of serological and molecular investigations

2017 ◽  
Vol 73 (9) ◽  
pp. 591-594 ◽  
Author(s):  
Jan Siemionek ◽  
Magdalena Załęska-Wawro ◽  
Konrad Przywara ◽  
Bolesław Gąsiorek ◽  
Anna Szczerba-Turek ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Disease Virus ◽  
Nucleotide Sequences ◽  
Gene Encoding ◽  
Vp2 Gene ◽  
Eradication Program ◽  
Aleutian Mink Disease Virus ◽  
Pcr Method ◽  
Sera Samples

The aim of the study was to evaluate the prevalence of seroreagents to Aleutian mink disease virus (AMDV) in minks. Sera samples from 1233 minks were tested by CIEP. The antibodies against AMDV were not found in the mink farms which continued the AMD eradication program. In farms where the eradication program had not been implemented a bad epizootic situation was detected. The percentage of seroreagents between 33.3% and 97.8% was noticed: in females 33.3% to 91.8% whereas in males it ranged from 61.5% to 97.8%. The PCR method was used in 101 spleen samples to detect the presence of 11 new nucleotide sequences of VP2 gene, encoding the capsid protein of AMDV. All 11 amplicons are new variants of the VP2 AMDV gene isolated from minks in Poland.

scholarly journals SEQUENCE ANALYSIS OF 18s DNA OF Melosira sp., Dunaliella sp., Isochrysis sp. AND Porphyridium sp.

2015 ◽  
Vol 2 (1) ◽  
pp. 592
Author(s):  
Lucia Kusumawati ◽  
Ruben Wahyudi ◽  
Reinhard Pinontoan ◽  
Maria Gorreti Lily Panggabean
Keyword(s):  
Sequence Analysis ◽  
Phylogenetic Tree ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Morphological Characters ◽  
Rdna Sequences ◽  
Pcr Products ◽  
18S Rdna Sequences ◽  
Pcr Method ◽  
High Level

<p>Phytoplankton has high level of biodiversity. In previous years phytoplankton was identified by their morphological characters. However, their morphology might change in different environments. These difficulties can be overcome by comparing their 18S rDNA sequences. This research is aimed to verify the identity of Melosira sp., Dunaliella sp., Isochrysis sp. and Porphyridium sp. Here, PCR method was used to amplify 18s DNA sequences. Three primer pairs were used, i.e. 18S-F and 18S-R; 501F and 1700R; 18S-2F and 18S-2R. PCR products were sequenced. MEGA5 was used to make phylogenetic tree. Genus verification for Isochrysis sp., Dunaliella sp. and Melosira sp. were conducted successfully using Blast and phylogenetic tree. 18s DNA sequence of Porphyridium sp. shows an interesting result and needs further verification.</p><p><br /><strong>Keywords</strong>: Phytoplankton, Melosira sp., Dunaliella sp., Isochrysis sp., Porphyridium sp.</p>

scholarly journals Evidence of Unique Genotypes of Beak and Feather Disease Virus in Southern Africa

2004 ◽  
Vol 78 (17) ◽  
pp. 9277-9284 ◽  
Author(s):  
Livio Heath ◽  
Darren P. Martin ◽  
Louise Warburton ◽  
Mike Perrin ◽  
William Horsfield ◽  
...  
Keyword(s):  
Disease Virus ◽  
Southern Africa ◽  
Nucleotide Diversity ◽  
Average Distance ◽  
Point Mutations ◽  
Clinical Signs ◽  
Pcr Amplification ◽  
Genome Sequences ◽  
Distinct Lineage ◽  
Feather Disease Virus

ABSTRACT Psittacine beak and feather disease (PBFD), caused by Beak and feather disease virus (BFDV), is the most significant infectious disease in psittacines. PBFD is thought to have originated in Australia but is now found worldwide; in Africa, it threatens the survival of the indigenous endangered Cape parrot and the vulnerable black-cheeked lovebird. We investigated the genetic diversity of putative BFDVs from southern Africa. Feathers and heparinized blood samples were collected from 27 birds representing 9 psittacine species, all showing clinical signs of PBFD. DNA extracted from these samples was used for PCR amplification of the putative BFDV coat protein (CP) gene. The nucleotide sequences of the CP genes of 19 unique BFDV isolates were determined and compared with the 24 previously described sequences of BFDV isolates from Australasia and America. Phylogenetic analysis revealed eight BFDV lineages, with the southern African isolates representing at least three distinctly unique genotypes; 10 complete genome sequences were determined, representing at least one of every distinct lineage. The nucleotide diversity of the southern African isolates was calculated to be 6.4% and is comparable to that found in Australia and New Zealand. BFDVs in southern Africa have, however, diverged substantially from viruses found in other parts of the world, as the average distance between the southern African isolates and BFDV isolates from Australia ranged from 8.3 to 10.8%. In addition to point mutations, recombination was found to contribute substantially to the level of genetic variation among BFDVs, with evidence of recombination in all but one of the genomes analyzed.

scholarly journals ISOLATION AND PHYLOGENETIC ANALYSIS OF FELINE CALICIVIRUS IN SIBERIA

2018 ◽  
Vol 63 (6) ◽  
pp. 268-274
Author(s):  
T. I. Glotova ◽  
O. V. Semenova ◽  
A. A. Nikonova ◽  
A. G. Glotov ◽  
Y. V. Vyatkin ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Acute Infection ◽  
Clinical Signs ◽  
Systemic Infection ◽  
Geographic Region ◽  
Nucleotide Sequences ◽  
Feline Calicivirus ◽  
Group Method ◽  
Reading Frame ◽  
Close Relationship

The results of the study of the distribution of calicivirus infection in a population of domestic cats of different breeds, contained individually or the group method, the virus isolation in the cell culture and a comparative phylogenetic analysis of their nucleotide sequences with published sequences of reference field and vaccine strains of Feline calicivirus (FCV) from other countries: USA, Germany, Japan, China and Korea are presented. Clinical signs of infection were found in 14.3% of the animals examined. After several passages in the primary kidney cells of the kitten embryo, seven cytopathogenic isolates FCV were isolated: 1 - from a cat with an acute infection, 5 - subclinical infection, 1 - systemic infection. They were adapted to continuous FK-81 cells in which they reached a maximum infectious activity of 10.0 ± 1.15 lg TCD 50 / cm3. Based on the sequence analysis of the open reading frame 2 region of the viral genome Eshli strain showed a close relationship with strain KM016908 from China with the identity of the nucleotide sequences between them of 81.0%. The results of the investigations showed that FCV isolates obtained from animals on the territory of Siberia are genetically different from strains included to imported vaccines used to prevent disease in Russian Federation and also among themselves. This causes a decrease in the effectiveness of preventive measures. In nurseries that do not have contacts and connections between themselves but located in the same geographic region FCV populations may have some genetic differences. A close relationship of some field isolates with strains from other countries geographically located so far from the Siberian region has been revealed. Studies on the molecular epizootology of caliciviruses are important in the development of test systems and the monitoring of the spread of strains in Russia.

Sign in / Sign up

Export Citation Format

Share Document