scholarly journals Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 2003
Author(s):  
Stephen D. Grant ◽  
Gemma S. Cairns ◽  
Jordan Wistuba ◽  
Brian R. Patton
Keyword(s):  
Fluorescence Imaging ◽  
Low Cost ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Entire System ◽  
3D Printed ◽  
Resolution Imaging ◽  
Cost Components ◽  
Better Than

We report on a 3D printed microscope, based on a design by the Openflexure project, that uses low cost components to perform fluorescence imaging. The system is sufficiently sensitive and mechanically stable to allow the use of the Super Resolution Radial Fluctuations algorithm to obtain images with resolution better than the diffraction limit. Due to the low-cost components, the entire system can be built for approximately $1200.

scholarly journals Fluorescence imaging with tailored light

Nanophotonics ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 2111-2128 ◽  
Author(s):  
Jialei Tang ◽  
Jinhan Ren ◽  
Kyu Young Han
Keyword(s):  
Fluorescence Imaging ◽  
Imaging Techniques ◽  
Super Resolution ◽  
Optical Parameters ◽  
Propagation Angle ◽  
Resolution Imaging ◽  
Time Observation ◽  
Living Organisms ◽  
Real Time Observation

AbstractFluorescence microscopy has long been a valuable tool for biological and medical imaging. Control of optical parameters such as the amplitude, phase, polarization, and propagation angle of light gives fluorescence imaging great capabilities ranging from super-resolution imaging to long-term real-time observation of living organisms. In this review, we discuss current fluorescence imaging techniques in terms of the use of tailored or structured light for the sample illumination and fluorescence detection, providing a clear overview of their working principles and capabilities.

Super-resolution imaging of SERS hot spots

2014 ◽  
Vol 43 (11) ◽  
pp. 3854-3864 ◽  
Author(s):  
Katherine A. Willets
Keyword(s):  
Hot Spots ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Resolution Imaging

Super-resolution imaging defeats the diffraction-limit of light, allowing the spatial origin and intensity of SERS signals to be determined with <5 nm resolution.

0.1THz super-resolution imaging based on 3D printed confocal waveguides

2020 ◽  
Vol 459 ◽  
pp. 124896 ◽  
Author(s):  
Ting Yu ◽  
Xu Zuo ◽  
Weiwei Liu ◽  
Cheng Gong
Keyword(s):  
Super Resolution ◽  
3D Printed ◽  
Resolution Imaging

scholarly journals Focus on Super-Resolution Imaging with Direct Stochastic Optical Reconstruction Microscopy (dSTORM)

2014 ◽  
Vol 67 (2) ◽  
pp. 179 ◽  
Author(s):  
Donna R. Whelan ◽  
Thorge Holm ◽  
Markus Sauer ◽  
Toby D. M. Bell
Keyword(s):  
Cell Biology ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Stochastic Optical Reconstruction Microscopy ◽  
Resolution Imaging ◽  
Optical Reconstruction ◽  
Set Up ◽  
Microscopy Techniques ◽  
Super Resolution Microscopy ◽  
Microscopic Techniques

The last decade has seen the development of several microscopic techniques capable of achieving spatial resolutions that are well below the diffraction limit of light. These techniques, collectively referred to as ‘super-resolution’ microscopy, are now finding wide use, particularly in cell biology, routinely generating fluorescence images with resolutions in the order of tens of nanometres. In this highlight, we focus on direct Stochastic Optical Reconstruction Microscopy or dSTORM, one of the localisation super-resolution fluorescence microscopy techniques that are founded on the detection of fluorescence emissions from single molecules. We detail how, with minimal assemblage, a highly functional and versatile dSTORM set-up can be built from ‘off-the-shelf’ components at quite a modest budget, especially when compared with the current cost of commercial systems. We also present some typical super-resolution images of microtubules and actin filaments within cells and discuss sample preparation and labelling methods.

scholarly journals Democratising “Microscopi”: a 3D printed automated XYZT fluorescence imaging system for teaching, outreach and fieldwork

2020 ◽  
Author(s):  
Matthew Wincott ◽  
Andrew Jefferson ◽  
Ian M. Dobbie ◽  
Martin J. Booth ◽  
Ilan Davis ◽  
...  
Keyword(s):  
Open Source ◽  
Fluorescence Imaging ◽  
Imaging System ◽  
Low Cost ◽  
Dark Field ◽  
List Type ◽  
Image Capture ◽  
Fluorescence Imaging System ◽  
3D Printed ◽  
Open Source Hardware

ABSTRACTCommercial fluorescence microscope stands and fully automated XYZt fluorescence imaging systems are generally beyond the limited budgets available for teaching and outreach. We have addressed this problem by developing “Microscopi”, an accessible, affordable, DIY automated imaging system that is built from 3D printed and commodity off-the-shelf hardware, including electro-mechanical, computer and optical components. Our design features automated sample navigation and image capture with a simple web-based graphical user interface, accessible with a tablet or other mobile device. The light path can easily be switched between different imaging modalities. The open source Python-based control software allows the hardware to be driven as an integrated imaging system. Furthermore, the microscope is fully customisable, which also enhances its value as a learning tool. Here, we describe the basic design and demonstrate imaging performance for a range of easily sourced specimens.HighlightsPortable, low cost, self-build from 3D printed and commodity componentsMultimodal imaging: bright field, dark field, pseudo-phase and fluorescenceAutomated XYZt imaging from a tablet or smartphone via a simple GUIWide ranging applications in teaching, outreach and fieldworkOpen source hardware and software design, allowing user modification

scholarly journals From 2D to 3D super-resolution imaging through glass microspheres -INVITED

2020 ◽  
Vol 238 ◽  
pp. 06002
Author(s):  
Stephane Perrin ◽  
Sylvain Lecler ◽  
Paul Montgomery
Keyword(s):  
3D Reconstruction ◽  
Imaging Technique ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Label Free ◽  
Magnification Factor ◽  
Glass Microspheres ◽  
Full Field ◽  
Resolution Imaging ◽  
2D To 3D

Microsphere-assisted microscopy is a new imaging technique which allows the diffraction limit to be overcome using transparent microspheres. It makes it possible to reach a resolution of up to 100 nm in air while being label-free and full-field. An overview of the imaging technique is presented showing the influence of the photonic jet on the image nature and the unconventional behaviour of the magnification factor. Moreover, interferometry through microspheres is demonstrated for the 3D reconstruction of nanoelements.

scholarly journals Ptychographic modulation engine: a low-cost DIY microscope add-on for coherent super-resolution imaging

2019 ◽  
Vol 53 (1) ◽  
pp. 014005 ◽  
Author(s):  
Zichao Bian ◽  
Shaowei Jiang ◽  
Pengming Song ◽  
He Zhang ◽  
Pouria Hoveida ◽  
...  
Keyword(s):  
Low Cost ◽  
Super Resolution ◽  
Resolution Imaging

scholarly journals Multilevel phase supercritical lens fabricated by synergistic optical lithography

Nanophotonics ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 1469-1477 ◽  
Author(s):  
Wei Fang ◽  
Jian Lei ◽  
Pengda Zhang ◽  
Fei Qin ◽  
Meiling Jiang ◽  
...  
Keyword(s):  
Numerical Aperture ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Optical Diffraction ◽  
Energy Concentration ◽  
Focal Region ◽  
Movement Trajectory ◽  
Label Free ◽  
Laser Fabrication ◽  
Resolution Imaging

AbstractThe advent of planar metalenses, including the super-oscillatory lens (SOL) and the supercritical lens (SCL) with distinctive interference properties, has profoundly impacted on the long-lasting perception of the far-field optical diffraction limit. In spite of its conspicuous success in achieving marvelously small focal spots, the planar metalens still faces tough design and fabrication challenges to realize high focusing efficiency. In this work, we demonstrated a dual-mode laser fabrication technique based on two-photon polymerization for realizing the multilevel phase SCL with focusing efficiency spiking. Synergistically controlling two types of movement trajectory, which is implemented with the piezo stage and the scanning galvo mirror, enables the fabrication of complicated structures with sub-diffraction-limit feature size. By utilizing such advantage, SCLs with discretized multilevel phase configurations are explicitly patterned. The experimental characterization results have shown that a four-level phase SCL can focus light into a sub-diffraction-limit spot with the lateral size of 0.41 λ/NA (NA is the numerical aperture), while achieve the focal spot intensity and the energy concentration ratio in the focal region 7.2 times and 3 times that of the traditional binary amplitude-type SCL with the same optimization conditions, respectively. Our results may release the application obstacles for the sub-diffraction-limit planar metalens and enable major advances in the fields from label-free optical super-resolution imaging to high precision laser fabrication.

scholarly journals Super-Resolution Imaging with Patchy Microspheres

Photonics ◽  
2021 ◽  
Vol 8 (11) ◽  
pp. 513
Author(s):  
Qingqing Shang ◽  
Fen Tang ◽  
Lingya Yu ◽  
Hamid Oubaha ◽  
Darwin Caina ◽  
...  
Keyword(s):  
Colloidal Particle ◽  
Near Field ◽  
Super Resolution ◽  
Incident Beam ◽  
Diffraction Limit ◽  
Beam Forming ◽  
Feature Size ◽  
Disc Surface ◽  
Promising Tool ◽  
Resolution Imaging

The diffraction limit is a fundamental barrier in optical microscopy, which restricts the smallest resolvable feature size of a microscopic system. Microsphere-based microscopy has proven to be a promising tool for challenging the diffraction limit. Nevertheless, the microspheres have a low imaging contrast in air, which hinders the application of this technique. In this work, we demonstrate that this challenge can be effectively overcome by using partially Ag-plated microspheres. The deposited Ag film acts as an aperture stop that blocks a portion of the incident beam, forming a photonic hook and an oblique near-field illumination. Such a photonic hook significantly enhanced the imaging contrast of the system, as experimentally verified by imaging the Blu-ray disc surface and colloidal particle arrays.

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