scholarly journals Prevalence of Giardia lamblia in stool samples of diarrhea patients in Quetta, Pakistan

2018 ◽  
Vol 12 (3) ◽  
pp. 116-121
Keyword(s):  
Giardia Lamblia ◽  
Stool Samples

An Epidemiological, Diagnostic and Therapeutic Study of Giardia lamblia in Anbar Province – Iraq

2019 ◽  
Vol 9 (01) ◽  
pp. 39-45
Author(s):  
Suad Shallal Shahatha
Keyword(s):  
Teaching Hospital ◽  
Rural Areas ◽  
Giardia Lamblia ◽  
Urban Areas ◽  
Culture Media ◽  
Age Groups ◽  
Stool Samples ◽  
Women And Children ◽  
Teucrium Polium L ◽  
Therapeutic Study

This study was carried out to investigate the epidemiology of Giardia lamblia parasites in patients who visited some of the hospitals in Anbar province, which included (Fallujah Teaching Hospital, Ramadi Teaching Hospital, Ramadi Teaching Hospital for Women and Children and Hit Hospital) during by examining 864 stool samples in a direct examination method, The results revealed the infection rate was 41.7 % and the percentage of infection among males 47.8% is higher than that of females 35.4% with significant differences (p≤0.05). The age groups (1-9) years recorded the highest rates 55.4% and the lowest rate 13.6% in the age group (40-49) years. The highest rate of infection was 62.5% during the month of June, while the month of October was the lowest rate 5% and significant differences. The incidence rate in rural areas was 50.6% higher than in the urban areas 32.5%. The study also included the effect of Teucrium polium L. on the parasite in the culture media HSP-1, the concentrations of 0.5-3 mg / mL significantly affected Giardia, it was noted whenever the greater the concentration, the greater the effect during different treatment periods (1-4) days, as the highest concentration 3 mg/ml killed all Giardia parasites on the fourth day of treatment.

Prevalence of Entamoeba histolytica and Giardia lamblia in Children in Kadhmiyah Hospital

2012 ◽  
Vol 36 (1) ◽  
pp. 1-5
Author(s):  
Amjed Qays Ibrahim
Keyword(s):  
Entamoeba Histolytica ◽  
Giardia Lamblia ◽  
Age Group ◽  
Significant Relation ◽  
Female Ratio ◽  
Male Ratio ◽  
Infectivity Rate ◽  
Stool Samples

In this study we collect 1520 stool samples during the period from September to December 2010 from children whom their ages between 1 month - 12 years. The results showed that the total infection of Entamoeba histolytica was 9.80% , and Giardia lamblia was 1.77%. And the male ratio that infected with Entamoeba histolytica was 9.83% , while the female ratio was 9.74%; and the male infected with Giardia lamblia was 1.51% , while the female ratio was 2.18%. The result showed that the high average of infection with Entamoeba histolytica and Giardia lamblia in age group from 1 month to 2 years. And there is no significance difference between gender and infectivity rate of Entamoeba histolytica and Giardia lamblia under P≤0.05. Also it showed that there were significant relation between Age group and infectivity rate of Entamoeba histolytica and Giardia lamblia.

scholarly journals Detection of Cryptosporidium spp and other intestinal parasites in children with acute diarrhea and severe dehydration in Rio de Janeiro

2007 ◽  
Vol 40 (3) ◽  
pp. 346-348 ◽  
Author(s):  
Filipe Anibal Carvalho-Costa ◽  
Alessandra Queiroga Gonçalves ◽  
Sandra Laranjeira Lassance ◽  
Carla Pontes de Albuquerque ◽  
José Paulo Gagliardi Leite ◽  
...  
Keyword(s):  
Giardia Lamblia ◽  
Intestinal Parasites ◽  
Blue Staining ◽  
Pediatric Hospital ◽  
Severe Dehydration ◽  
Enterobius Vermicularis ◽  
Blastocystis Hominis ◽  
Entamoeba Dispar ◽  
Cryptosporidium Spp ◽  
Stool Samples

The objective of the present study was to estimate the frequency of infection by Cryptosporidium spp and other intestinal parasites in dehydrated children with gastroenteritis who were admitted to a pediatric hospital. Stool examinations from 218 children were performed. Cryptosporidium spp was identified in eighteen out of 193 stool samples (9.3%) subjected to safranin-methylene blue staining. Giardia lamblia was detected in ten out of 213 (4.7%) samples examined via the direct or Ritchie methods. Other parasites identified were Ascaris lumbricoides (4.2%), Blastocystis hominis (1.4%), Entamoeba coli (0.9%), Entamoeba histolytica/Entamoeba dispar (0.5%), Endolimax nana (0.5%), Trichuris trichiura (0.5%) and Enterobius vermicularis (0.5%).

scholarly journals Enteroparasitic occurrence in fecal samples analyzed at the University of Western São Paulo-UNOESTE clinical laboratory, Presidente Prudente, São Paulo State, Brazil

2004 ◽  
Vol 46 (5) ◽  
pp. 243-248 ◽  
Author(s):  
Nair Toshiko Tashima ◽  
Maria Jacira Silva Simões
Keyword(s):  
Giardia Lamblia ◽  
Clinical Laboratory ◽  
Clinical Signs ◽  
Data Bank ◽  
Sao Paulo ◽  
São Paulo ◽  
Bank Structure ◽  
Stool Samples ◽  
Medical Attendance ◽  
The University

This study aims to analyze the enteroparasitic occurrence in children from 0 to 12 years old consulted at the University of western São Paulo Clinical Laboratory, Presidente Prudente, SP, Brazil, in relation to the socioeconomic profile of the attended children. Stool samples were examined and a questionnaire was applied with the objective of knowing the patient's age, sex, medical attendance, characteristic of the habitation, provisioning of water, dejection and domestic waste fates, use of footwear and clinical signs. The software EPI INFO 6 (Version 6.04b) was used for the elaboration of the data bank structure and analysis after previous data codification. Among 1,000 children analyzed, as many as 21.3% presented some kind of parasite. The most frequent protozoan was Giardia lamblia (7.3%) followed by Entamoeba coli (3.9%). The most frequent helminth was Enterobius vermicularis (1.9%) followed by Hymenolepis nana (0.5%). The most frequent protozoan association was Giardia lamblia / Entamoeba coli (0.9%).

scholarly journals Genotype-specific detection of Giardia lamblia in stool samples of diarrhoeal and non-diarrhoeal patients in Dhaka, Bangladesh

1970 ◽  
Vol 20 (2) ◽  
pp. 183-189 ◽  
Author(s):  
Md Masud Alam ◽  
Mohammad Ilias ◽  
Md Abdullah Siddique ◽  
Md Mamun Kabir ◽  
Farida Nazib ◽  
...  
Keyword(s):  
Positive Result ◽  
Real Time ◽  
Real Time Pcr ◽  
Giardia Lamblia ◽  
Specific Antigen ◽  
Diarrhoeal Disease ◽  
Giardia Cyst ◽  
Positive Stool ◽  
Stool Samples ◽  
Assemblage B

Two major genotypic assemblages (A and B) of Giardia lamblia infect humans. A single-vessel multiplex real-time PCR assay was used that genotypes Giardia infections into assemblages A and/or B directly from fecal samples. In this study, 157 diarrhoeal (symptomatic) and non-diarrhoeal (asymptomatic) stool samples collected from the International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B) and Bangabandhu Sheikh Mujib Medical University (BSMMU) hospital, respectively were analyzed to determine whether an association exists between infections with G. lamblia assemblages A or B and diarrhea in Bangladesh. Of the 157 stool samples, Giardia cysts were observed in 35 by microscopy and 127 showed positive result for Giardia cyst specific antigen. The 127 ELISA positive samples were assayed for genotyping by real?time polymerase chain reaction. Of the 117 real-time PCR positive stool samples, 15 were positive for G. lamblia assemblage A, 96 were positive for assemblage B and 6 samples showed positive result for both G. lamblia assemblage A and B infections. Higher ratios for diarrhea were observed for assemblage A infections, whereas higher parasite DNA loads and a higher overall rate were observed for assemblage B infections in both diarrhoeal and non-diarrhoeal patients. Real-time PCR is, therefore, useful as an additional test supplementary to microscopy or enzyme immunoassay to detect genotypes of Giardia. Key words: Giardia lamblia; Genotypes; Multiplex real-time PCR; Immunoassay DOI: http://dx.doi.org/10.3329/dujbs.v20i2.8979 DUJBS 2011; 20(2): 183-189

scholarly journals Malaria and intestinal parasitosis among children presenting to the Paediatric Centre in Sana’a, Yemen

2003 ◽  
Vol 9 (5-6) ◽  
pp. 1048-1053
Author(s):  
A. A. Azazy ◽  
Y. A. Raja'a
Keyword(s):  
Giardia Lamblia ◽  
Malaria Parasite ◽  
Health Centre ◽  
Schistosome Infection ◽  
Blood Samples ◽  
Intestinal Parasitosis ◽  
Stool Samples ◽  
Common Infection ◽  
Paediatric Health ◽  
Direct Life Cycle

We studied the profile of malaria and intestinal parasitosis among children presenting to the Paediatric Health Centre in Sana’a from January 1998 to December 2000. In stool samples from 9014 children, Ascaris lumbricoides, Entamoeba histolytica, Giardia lamblia and Trichuris trichiura were the most common. Infection with parasites of direct life-cycle were similar in boys and girls. Schistosome infection was significantly higher in boys than girls, but girls were more infected with ascariasis. The only species of malaria parasite found in blood samples from 753 children with suspected malaria was Plasmodium falciparum, with the highest rates in April-June. The majority of positive cases were Yemeni children, but 10.8% were Sudanese or Ethiopian

scholarly journals A five year retrospective analysis of common intestinal parasites at Poly Health Center, Gondar, Northwest Ethiopia

2019 ◽  
Author(s):  
Meseret Ayelgn ◽  
Ligabaw Worku ◽  
Getachew Ferede ◽  
Yitayih Wondimeneh
Keyword(s):  
Health Center ◽  
Giardia Lamblia ◽  
Intestinal Parasite ◽  
Intestinal Parasites ◽  
Age Groups ◽  
Northwest Ethiopia ◽  
P Value ◽  
Wet Mount ◽  
Parasite Infections ◽  
Stool Samples

Abstract Objective: Intestinal parasites are present throughout the world in varying degrees of prevalence due to many factors. The aim of this study was to determine the five-year trend prevalence of intestinal prevalence among patients who had been suspected for intestinal parasite infections. A retrospective study was conducted from 2009 to 2013 at Poly Health Center Gondar, Northwest Ethiopia. Samples were examined using direct saline wet mount methods. Statistical analysis was done using SPSS version 20 software and a P-value of < 0.05 was considered statistically significant. The results were presented in tables and graphs. Results: During the study period, a total of 13,329 stool samples were requested for intestinal parasite diagnose and 5510 (41.3%) laboratory-confirmed cases were reported with a fluctuating trend. Ten different parasites were reported in each year with Entamoeba histolytica/dispar (16.8%) being the predominant parasite followed by Giardia lamblia (11.4%) and Ascaris lumbricoides (6.7%). Both males (49%) and females (51%) were equally affected (P=0.14). The intestinal parasite was reported in all age groups in the area but the highest and the lowest prevalence were reported in age groups of 20-29 years and 40-49 years, respectively (26.5% vs 6.4%) (P < 0.001).

Stool Survey of Asymptomatic Diapered Children in Day Care

PEDIATRICS ◽  
1994 ◽  
Vol 94 (6) ◽  
pp. 1026-1027
Author(s):  
Michele M. Ginsberg ◽  
Kathleen Keenan ◽  
Muriel Thompson ◽  
Bronwen Anders
Keyword(s):  
Giardia Lamblia ◽  
Day Care ◽  
Asymptomatic Infection ◽  
Day Care Centers ◽  
Enteric Diseases ◽  
Department Of Health ◽  
Asymptomatic Children ◽  
Stool Samples ◽  
Stool Specimens ◽  
Stool Cultures

A survey of diaper-wearing children in community-day-care centers was conducted by the San Diego Department of Health Services during the period February-April 1986 to determine the presence of enteric pathogens in asymptomatic children. Written consent to collect stool samples from diapers was obtained from parents of children at four preschools. No reportable enteric diseases or outbreaks had occurred at the schools in the preceding year. For each child, the preschool teachers maintained a record of symptoms (eg, diarrhea, fever) during the week of stool collection. Stool specimens were collected from 63 diapered children. The samples were examined for parasites and cultured for bacteria and viruses. Giardia lamblia was identified in 18% to 32% of stool samples collected from children at three of the four centers. Prevalence of Giardia varied with the age of the child (0% age &lt;12 months to 33% age &lt;23 months). Microscopic examinations for Cryptosporidium and pathogenic amoeba were negative. Stool cultures were negative for Salmonella, Shigella, and Campylobacter. The prevalence of adenoviruses identified by viral cultures ranged from 32% to 80% at four sites. Echovirus was isolated from 36% of samples at one center. All stools were negative for rotavirus. Giardia was identified in the stools of 22% of children in day care in the absence of diarrhea or of concurrent illness. These findings indicate that asymptomatic infection with Giardia occurs commonly among children attending day-care centers. The lack of any documented outbreak or symptoms of enteric disease among attendees suggests that these asymptomatic children with Giardia need not be excluded from day care.

Bacteriological And Parasitological Study Of Acute Gastroenteritis Among Patients Visiting Sukraraj Tropical And Infectious Disease Hospital (STIDH), Teku, Kathmandu

2020 ◽  
Author(s):  
Sanjeena Jangam ◽  
Nisha Lamsal ◽  
Prashansha Sharma ◽  
Najma Makaju ◽  
Beena Mali ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Giardia Lamblia ◽  
Shigella Flexneri ◽  
Public Health Problem ◽  
Extensive Study ◽  
Personal Hygiene ◽  
Resistance Pattern ◽  
Parasitic Infections ◽  
Major Public Health Problem ◽  
Stool Samples

Abstract BACKGROUND: Gastroenteritisis a major public-health problem in developing nations like Nepal, where communities are still ravaged by poverty, poor sanitation, poor personal hygiene, and poor water supplies.The objective of our study was to find the bacterial and parasitic agents responsible for causing gastroenteritis.METHODOLOGY: A hospital based prospective study was carried out for 3 months in Bacteriology and Parasitology section of STIDH. Stool samples received in respective section from patients with gastroenteritis were included in the study. Standard Microbiological Guidelines were employed for collection and processing of samples, followed by Isolation, Identification and Antimicrobial Susceptibility Testing of bacterial isolates. Normal saline and Iodine preparation were done for microscopic examination of parasites.RESULTS: Among 421 stool samples processed in Bacteriology section, 28(6.65%) showed bacterial growth with 15(53.57%) Shigella flexneri , 4(14.28%) S higella sonnei and 9(32.14%) Salmonella typhimurium . Ceftriaxone and Nalidixic acid were found to be the most Sensitive and Resistant antibiotic for Shigella spp. and Salmonella typhimurium. Similarly, in parasitology section, microscopic observation of 648 stool samples showed 136(20.98%) cysts of Entamoeba histolytica , 3(0.46%) cysts of Giardia lamblia , 2(0.30%) trophozoites of Giardia lamblia , 1 (0.15%) larva of Strongyloides stercoralis , 1(0.15%) ova of hookworm and 1(0.15%) ova of Ascaris lumbricoides .CONCLUSION: The study revealed Shigella species as the predominant bacterial agent with S. flexneri being the major one. Hence, extensive study of shigellosis with greater emphasis on resistance pattern of different group of antibiotics is essential in such hospital. Similarly, parasitic infections are found in significant amount. Hence, concerned hospital authorities and government official’s needs to take strict action in contending such infections.

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