scholarly journals PHOTOSYNTHETIC PIGMENT CONTENT AS A CONSEQUENTAL RESISTANCE SIGN OF POTATO VARIETIES TO HIGH TEMPERATURE AND MOISTURE LACK

2020 ◽  
Vol 5 (2) ◽  
pp. 8-13
Author(s):  
Aleksei Bakunov ◽  
Aleksei Milekhin ◽  
Sergei Rubtsov ◽  
Sergei Shevchenko
Keyword(s):  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Photosynthetic Pigment ◽  
Negative Relationship ◽  
Average Concentration ◽  
Potato Yield ◽  
Chlorophyll B ◽  
High Concentration ◽  
Middle Volga Region ◽  
Plant Nursery

The aim of the research is increasing potato yield in dry conditions of the Middle Volga region. The research was carried out on the experimental field of the Samara Scientific Research Institute of Agriculture – branch of the Sa-mara Research Center of the Russian Academy of Sciences. The variety testing plant nursery included 28 varieties of potatoes of domestic selection. Udacha, Zhigulevsky, Arosa and Gala breeds were the standard varieties. The plant material was planted out in two replications with 25 plants in each. To determine the content of photosynthetic pigments, extracts were prepared from ten potato leaves of each breed in 100% acetone. Measurements were per-formed by spectrophotometry. To measure the amount of chlorophyll a, the wavelength of 665 nm, chlorophyll b – 649 nm, and carotenoids – 440 nm were used. The concentration of pigments was determined by using Wettstein formulas. When harvesting, the potato yield was taken into account. The average concentration of chlorophyll a in the studied breeds was 0.92 mg/g, the average concentration of chlorophyll b was 0.62 mg/g, and one of carote-noids was 0.27 mg/g. Potato breeds with high concentration of photosynthetic pigments were identified. A reliable average dependence of the yield of potato breeds on the concentration of chlorophyll b in plants was revealed. The correlation coefficient was 0.42. There is no reliable dependence tested of yield on the concentration of chlorophyll a and carotenoids. Potato productivity is associated with a significant negative relationship with the ratio of both a and b chlorophyll concentrations. The most highly productive varieties were characterized by a high content of two varieties of chlorophyll. A high concentration of chlorophyll b or a minimum ratio of chlorophyll a and b concentra-tions can be tentatively recommended as a consequential sign for identifying potato breeds that are highly adapted to high air temperature and insufficient moisture.

scholarly journals Variations of Chlorophyll Content at Abies alba and Nepeta pannonica Species According to Phenophase and Harvesting Area

2019 ◽  
Vol 52 (1) ◽  
pp. 74-78
Author(s):  
S. Buhăianu ◽  
Doina Carmen Jităreanu
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Photosynthetic Activity ◽  
Abies Alba ◽  
Chlorophyll B ◽  
Green Color ◽  
Yellow Coloration ◽  
Chlorophyll A And B ◽  
Chlorophyll A Content

Abstract Chlorophylls from plants are photosynthetic pigments. Their quantity offers valuable informations about photosynthetic activity, growing and developing of plants. Photosynthetic pigments decrease quantitatively during senescence process or in stress conditions. The present study has been realized in laboratory conditions with material harvested from spontaneous flora. The purpose of this research was the investigation of variations of chlorophyll content from samples of biological material collected from Nepeta pannonica L. and Abies alba Mill. plants, from Câmpulung Moldovenesc and Cacica areas, Suceava county, Romania. The targeted phenophases were growth and flowering. There were realized acetonic extracts from samples for spectrophotometric determinations. Obtained data were processed to estabilish chlorophyll a and b content. There were observed that at Abies alba species, from both locations, the chlorophyll a content grew during flowering phenophase, while the chlorophyll b content had little variations. At Nepeta pannonica species, the chlorophyll a and b content decreased visibly during the flowering, due to stress. Leaves of plants from this species presented a intense green color in the growing phenophase, while during flowering phenophase they had a purple or yellow coloration. Obtained results revealed a different dynamics of chlorophyll content at studied species.

Interactions between Photosynthetic Pigments Bound to Lipid and Protein Particles. Spectroscopic Properties

1979 ◽  
Vol 34 (7-8) ◽  
pp. 582-587
Author(s):  
Framçoise Techy ◽  
Monique Dinant ◽  
Jacques Aghion
Keyword(s):  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Relative Concentration ◽  
Spectroscopic Properties ◽  
Chlorophyll B ◽  
Pheophytin A ◽  
Chlorophylls A And B ◽  
Protein Particles ◽  
Β Carotene

Abstract The spectroscopic (visible) properties of pigment-bearing lipid and protein particles extract­ ed from milk show that: 1) chlorophylls a and b bound to separate particles can form aggregates provided their relative concentration is high enough. Neither pheophytin a nor β-carotene, in the same conditions, form observable aggregates. 2) Chlorophylls a and b can co-aggregate when they are bound to the same particles. Pheophytin a as well as β-carotene seem to prevent the aggregation of chlorophyll a. β-carotene has no effect on the aggregation of chlorophyll b.

scholarly journals EDTA and nitric acid responses on nickel uptake, translocation factor and pigments on Spinacia oleracea L. replanted seedlings in hydroponic solution

2021 ◽  
Vol 25 (9) ◽  
pp. 1575-1580
Author(s):  
S. Abdulsalam ◽  
M.K. Yahaya ◽  
L.O. Habib ◽  
N.O. Ugbenyo
Keyword(s):  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Antioxidant Defense ◽  
Spinacia Oleracea ◽  
Translocation Factor ◽  
Antioxidant Defense System ◽  
Chlorophyll B ◽  
Defense System ◽  
Spinacia Oleracea L ◽  
Nickel Uptake

The effects of Na2EDTA and HNO3 on Ni2+ uptake by Spinacia oleracea seedlings replanted inhydroponic culture in a greenhouse was investigated. Eight week old seedlings, were exposed to various doses of Ni2+ (0, 1000, 2000, and 4000 mg/L) as NiSO4, at (0, 500 and 3000 mg/L) Na2EDTA and ( 0, 500 and 3000 mg /L) HNO3 in different combinations. There was a substantial increase in nickel uptake in chelated treatments (p < 0.05) compared to unchelated treatments of same concentrations of Ni2+. So, chelation enhanced Ni2+ uptake in S. oleracea. During the exposure, antioxidant defense system helped the plant to protect itself from the damage. Due to increasing nickel  uptake by the plant, the photosynthetic pigments (i.e chlorophyll a, chlorophyll b and Caretenoids) gradually declined. In this study, Spinacia oleracea Seedlings and contents of the photosynthetic pigments (chlorophyll a, chlorophyll b and Caretenoids) of both chelated and unchelated hydroponic treatments were investigated. Changes in photosynthetic pigments was significant (p < 0.05) with respect to addition of EDTA and HNO3 at different concentration to different concentrations of Ni2+ compared to unchelated treatments of same concentrations of Ni2+. The Ni2+ induced translocation factor was also determined which increased significantly (P < 0.05) with increasing Ni2+ concentrations.

scholarly journals Influence of Color Temperature of White LED Diodes and Illumination Intensity on the Content of Photosynthetic Pigments in Chlorella vulgaris Algae Cells

Proceedings ◽  
2019 ◽  
Vol 16 (1) ◽  
pp. 46
Author(s):  
Paweł Kondzior ◽  
Damian Tyniecki ◽  
Andrzej Butarewicz
Keyword(s):  
Organic Matter ◽  
Chlorophyll A ◽  
Chlorella Vulgaris ◽  
Photosynthetic Pigments ◽  
White Light ◽  
Illumination Intensity ◽  
Color Temperature ◽  
Chlorophyll B ◽  
Chl A ◽  
Warm White Light

The purpose of this paper is to determine the influence of color temperature of Light Emitting Diode (LED) diodes and illumination intensity on the content of photosynthetic pigments of chlorophyll a, chlorophyll b and carotenoids in Chlorella vulgaris algae cells. Choosing the right color temperature and intensity of illumination can favorably affect the growth of algae. In particular, it can contribute to the efficiency of the photosynthesis process and the amount of produced biomass from Chlorella vulgaris algae. In the spectrophotometric studies, the highest content of chlorophyll a, chlorophyll b and carotenoids was found in cultures illuminated with very cold white light (8500 K) with an intensity of 500 μmol/m2s. The highest measured content of chlorophyll a (Chl a) pigments was 48.29 mg/L, Chl b pigment was 23.25 mg/L and carotenoids pigment was 12.65 mg/L; the smallest content of pigments for Chl a (11.48 mg/L), Chl b (4.69 mg/L) and carotenoids (3.03 mg/L) was found in the sample illuminated with warm white light (3200 K) with an intensity of 50 μmol/m2s. The highest amount of dry organic matter amounting to 2.0 g/L was found in a sample illuminated with warm white light (3200 K) with an intensity of 250 μmol/m2s, then 1.91 g dry organic mass (DOM)/L for very cold white light with an intensity of 250 μmol/m2s, and 1.48 g DOM/L for very cold white light with an intensity of 50 μmol/m2s. The obtained results show that a higher content of photosynthetic pigments does not directly affect the increase of the amount of dry organic matter.

scholarly journals Short-term physiological responses to copper stress in Salvinia auriculata Aubl.

2014 ◽  
Vol 26 (3) ◽  
pp. 268-277 ◽  
Author(s):  
Andresa Lana Thomé Bizzo ◽  
Aline Chaves Intorne ◽  
Pollyana Honório Gomes ◽  
Marina Satika Suzuki ◽  
Bruno dos Santos Esteves
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Electrolyte Leakage ◽  
Physiological Responses ◽  
Reactive Oxygen ◽  
Copper Stress ◽  
Oxygen Species ◽  
Chlorophyll B

AIM: To evaluate, in a short-time exposure, the physiological responses of Salvinia auriculata Aubl. under different concentrations of Cu. METHODS: The plants were exposed to treatments with 0.01, 0.1, 1 and 10 mM of Cu in a period of 2 days. Then development variables of S. auriculata (weight, photosynthetic pigments, and soluble carbohydrate), lipid peroxidation (malondialdehyde, aldehydes, and electrolyte leakage) and production of antioxidants (anthocyanins, carotenoids, flavonoids, and proline) were evaluated. RESULTS: It was observed fresh weight reductions in concentrations above 1 mM of Cu. Chlorophyll a decreased with the increase of Cu concentrations unlike chlorophyll b. The ratio chlorophyll a / chlorophyll b was changed due to the degradation of photosynthetic pigments. The reductions of carotenoids were more pronounced than that of total chlorophyll. The values of electrolyte leakage ranged from 14 to 82 % and lipid peroxidation from 7 to 46 nmol.g-1. Flavonoids and soluble carbohydrates showed reductions with the increase of Cu concentration. Anthocyanins, phenolic compounds, and proline when subjected to 0.1 mM of Cu had increased, suggesting adaptability of plant stress caused directly by metal and reactive oxygen species. In higher concentrations, degradation and/or direct modifications of these molecules possibly occurred. CONCLUSIONS: The data suggest that S. auriculata is provided with an efficient mechanism against stress caused by Cu in the concentration of 0.1 mM. As for higher concentrations (1 and 10 mM), despite its role as micronutrients, Cu was toxic to the plant due to the redox behavior of this metal, which leads to the exacerbated formation of reactive oxygen species, inducing to severe damage such as biological membrane degradation and protein denaturation.

CO2 exchange, photosynthetic pigment composition, and cell ultrastructure of Sphagnum mosses during dehydration and subsequent rehydration

1996 ◽  
Vol 74 (5) ◽  
pp. 726-734 ◽  
Author(s):  
Renato Gerdol ◽  
Angelo Bonora ◽  
Rita Gualandri ◽  
Simonetta Pancaldi
Keyword(s):  
Photosynthetic Pigments ◽  
Photosynthetic Pigment ◽  
Co2 Exchange ◽  
Cell Ultrastructure ◽  
Carbon Gain ◽  
Controlled Environment ◽  
Chlorophyll B ◽  
Pigment Composition ◽  
Sphagnum Mosses ◽  
Water Holding

Sphagnum mosses of three different species (S. capillifolium, S. magellanicum, and S. fallax) were allowed to dry in a controlled environment. The three species lost water at different rates, but after 11 days of exposure to drying atmosphere all were dry and unable to photosynthesize. The chlorophyllose cells of all three species showed signs of alteration, mainly membrane shrinkage. Upon rehydration, concentrations of photosynthetic pigments (chlorophyll a to a greater extent than chlorophyll b) declined in tissues of S. magellanicum and especially in S. fallax. Sphagnum capillifolium and S. magellanicum resumed photosynthesis, although slowly, whereas S. fallax did not achieve a net carbon gain (most of its chlorophyllose cells were irreversibly damaged) after 7 days of rewetting. In the field, prolonged drought may alter the interspecific equilibria among coexisting Sphagnum species possessing different degrees of desiccation-tolerance and especially different water-holding abilities. Keywords: Sphagnum, photosynthesis, ultrastructure, photosynthetic pigments, dehydration, rehydration.

scholarly journals The balance of photosynthetic pigments pools and phenolic compounds in dwarf shrubs on the weakly disrupted territory

2019 ◽  
Vol 8 (3) ◽  
pp. 84-89
Author(s):  
Marina Vyacheslavovna Ustinova ◽  
Inessa Vyacheslavovna Kravchenko ◽  
Svetlana Nikolaevna Rusak ◽  
Diana Athamzhanovna Yadgarova
Keyword(s):  
Phenolic Compounds ◽  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Vaccinium Myrtillus ◽  
Chlorophyll B ◽  
Direct Average ◽  
Biochemical Assessment ◽  
B 31 ◽  
Vaccinium Myrtillus L ◽  
Khanty Mansi Autonomous

The paper identifies photosynthetic pigments pools balance and phenolic compounds in suffruticous species of Vaccinium myrtillus L. and Vaccinium vitis-idaea L. on the Tundrinsky bor territory (Khanty-Mansi Autonomous Okrug - Yugra) for the purpose of ecological and biochemical assessment of the valuable plant species status. The authors carry out a comparative analysis of the studied biochemical parameters (chlorophyll a , chlorophyll b , carotenoids and phenolic compounds) of two species leaves, collected from two sample plots similar in landscape and soil and hydrological conditions of the weakly disrupted territory of the Tundrinsky bor territory. The analysis of the suffruticous species leaves for chlorophyll content revealed the following distribution of chlorophyll pools: chlorophyll a - 64,96%, chlorophyll b - 35,04% for leaves of Vaccinium myrtillus and chlorophyll a - 68,28%, chlorophyll b - 31, 72% for leaves of Vaccinium vitis-idaea . The average content of carotenoids and phenolic compounds of Vaccinium myrtillus leaves was 0,62 0,11 mg/g and 14,18 1,65 mg/g, respectively, in Vaccinium vitis-idaea leaves - 0,52 0,12 mg/g and 18,79 2,25 mg/g, respectively. The correlation analysis showed the presence of a direct average strength correlation (r = 0,4) between the levels of chlorophyll and phenolic compounds in the leaves of Vaccinium vitis-idaea .

scholarly journals Magnetic Fields Induce Changes in Photosynthetic Pigments Content in Date Palm (Phoenix dactylifera L.) Seedlings

2009 ◽  
Vol 3 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Faten Dhawi ◽  
Jameel M. Al-Khayri
Keyword(s):  
Magnetic Field ◽  
Magnetic Fields ◽  
Chlorophyll A ◽  
Static Magnetic Field ◽  
Photosynthetic Pigments ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Alternating Magnetic Field ◽  
Chlorophyll B ◽  
The Impact

Growth, development and plants productivity are usually affected by photosynthetic pigments activity. Magnetic fields are known to induce biochemical changes and could be used as a stimulator for growth related reactions including affecting photosynthetic pigments. The impact of magnetic field strengths on chlorophyll and carotenoids were investigated in this study through the use of date palm (Phoenix dactylifera L.) seedlings. To study the effects of magnetic treatments on photosynthetic pigments, date palm seedlings were exposed to magnetic fields in two experiments. In the first experiment, seedlings were treated with static magnetic field at three levels of (10, 50 and 100 mT) and different durations (30, 60, 180, 240 and 360 min). At the second experiment, seedlings were treated with alternating magnetic field at 1.5 T for different durations (1, 5, 10 and 15 min). The photosynthetic pigments (chlorophyll a, chlorophyll b, carotenoids and total pigments) were assayed using spectrophotometric methods. Results indicated that pigments content (chlorophyll a, chlorophyll b, carotenoids and total pigments) was significantly increased under static magnetic field. The highest measurements were recorded at 100 mT, after 360 min of exposure. On the other hand, alternating magnetic field has decreased photosynthetic pigments content after 10 min of treatment with 1.5 T. Low magnetic field doses had a simulative effect on photosynthetic pigments whereas high doses had a negative effect. Chlorophyll a and carotenoids were more affected than chlorophyll b. Magnetic fields treatment could be used to enhance plant growth and productivity.

Ontogenetic Changes of the Pigment Composition of Leaves in Cyan Oil Flax Variety in the Steppe Zone Of Ukraine

2019 ◽  
pp. 44-53
Keyword(s):  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Steppe Zone ◽  
Chlorophyll B ◽  
Ontogenetic Changes ◽  
Stages Of Development ◽  
Stage Of Development ◽  
Wet Weight ◽  
Growing Conditions ◽  
Flax Variety

In recent years, oil flax has been very popular in Ukraine. Different spheres of the use of culture indicate good adaptive opportunities for growing oil flax in different regions of Ukraine. The aim of this work is to study the content and ratio of the main photosynthetic pigments of the flax variety of oilseed Cyan grown in ontogenesis. Plants were grown in natural open ground. The number of pigments was determined by spectrophotometric method. This is research data on the content of the main photosynthetic pigments in flax of Tsian. The content of chlorophyll a, b and carotenoids was studied at three stages of development: at the stage of 8-10 leaves, budding, flowering. It was established that the amount of chlorophyll a exceeds the content of chlorophyll b at all stages of development. An increase in the pigment content during ontogenesis to the flowering stage was revealed, with the exception of chlorophyll b. At the stage of the at the stage of 8-10 leaves in the Tsian variety, the amount of chlorophyll a ranged from min 754.74 ± 63.768 μg/g, max 1582.82 ± 126.973 μg/g wet weight, the amount of chlorophyll b ranging from min 203.23 ± 17.549 to max 602.24 ± 46.577 μg g wet weight, and carotenoids from min 220.99 ± 18.388 to max 409.94 ± 37.572 μg/g fresh weight, depending on the year of study. In the budding phase, the minimum content of chlorophyll a was min 1444.87 ± 105.771, and the maximum max 2027.34 ± 174.258 μg/g wet weight, chlorophyll b: from min 553.23 ± 48.345 to max 1733.69 ± 109.675 μg/g raw weight, and carotenoids: from min 354.81 ± 26.759 to max 402.63 ± 26.765 μg/g wet weight, depending on the year of study. In the flowering phase, the following indicators were observed: chlorophyll a content from min 1804.43 ± 164.425 to max 2370.56 ± 207.589 μg/g wet weight, chlorophyll b from min 1055.24 ± 89.562 μg/g wet weight to max 1649.21 ± 107.258 μg/g, and carotenoids from min 472.19 ± 36.141 μg/g to max 519.3322 ± 39.911 μg/g, depending on the year of study. The ratio of the number of chlorophylls and the sum of chlorophylls to carotenoids varies depending on the stage of development of the variety and growing conditions.

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