scholarly journals Influence of Color Temperature of White LED Diodes and Illumination Intensity on the Content of Photosynthetic Pigments in Chlorella vulgaris Algae Cells

Proceedings ◽  
2019 ◽  
Vol 16 (1) ◽  
pp. 46
Author(s):  
Paweł Kondzior ◽  
Damian Tyniecki ◽  
Andrzej Butarewicz
Keyword(s):  
Organic Matter ◽  
Chlorophyll A ◽  
Chlorella Vulgaris ◽  
Photosynthetic Pigments ◽  
White Light ◽  
Illumination Intensity ◽  
Color Temperature ◽  
Chlorophyll B ◽  
Chl A ◽  
Warm White Light

The purpose of this paper is to determine the influence of color temperature of Light Emitting Diode (LED) diodes and illumination intensity on the content of photosynthetic pigments of chlorophyll a, chlorophyll b and carotenoids in Chlorella vulgaris algae cells. Choosing the right color temperature and intensity of illumination can favorably affect the growth of algae. In particular, it can contribute to the efficiency of the photosynthesis process and the amount of produced biomass from Chlorella vulgaris algae. In the spectrophotometric studies, the highest content of chlorophyll a, chlorophyll b and carotenoids was found in cultures illuminated with very cold white light (8500 K) with an intensity of 500 μmol/m2s. The highest measured content of chlorophyll a (Chl a) pigments was 48.29 mg/L, Chl b pigment was 23.25 mg/L and carotenoids pigment was 12.65 mg/L; the smallest content of pigments for Chl a (11.48 mg/L), Chl b (4.69 mg/L) and carotenoids (3.03 mg/L) was found in the sample illuminated with warm white light (3200 K) with an intensity of 50 μmol/m2s. The highest amount of dry organic matter amounting to 2.0 g/L was found in a sample illuminated with warm white light (3200 K) with an intensity of 250 μmol/m2s, then 1.91 g dry organic mass (DOM)/L for very cold white light with an intensity of 250 μmol/m2s, and 1.48 g DOM/L for very cold white light with an intensity of 50 μmol/m2s. The obtained results show that a higher content of photosynthetic pigments does not directly affect the increase of the amount of dry organic matter.

scholarly journals Variations of Chlorophyll Content at Abies alba and Nepeta pannonica Species According to Phenophase and Harvesting Area

2019 ◽  
Vol 52 (1) ◽  
pp. 74-78
Author(s):  
S. Buhăianu ◽  
Doina Carmen Jităreanu
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Photosynthetic Activity ◽  
Abies Alba ◽  
Chlorophyll B ◽  
Green Color ◽  
Yellow Coloration ◽  
Chlorophyll A And B ◽  
Chlorophyll A Content

Abstract Chlorophylls from plants are photosynthetic pigments. Their quantity offers valuable informations about photosynthetic activity, growing and developing of plants. Photosynthetic pigments decrease quantitatively during senescence process or in stress conditions. The present study has been realized in laboratory conditions with material harvested from spontaneous flora. The purpose of this research was the investigation of variations of chlorophyll content from samples of biological material collected from Nepeta pannonica L. and Abies alba Mill. plants, from Câmpulung Moldovenesc and Cacica areas, Suceava county, Romania. The targeted phenophases were growth and flowering. There were realized acetonic extracts from samples for spectrophotometric determinations. Obtained data were processed to estabilish chlorophyll a and b content. There were observed that at Abies alba species, from both locations, the chlorophyll a content grew during flowering phenophase, while the chlorophyll b content had little variations. At Nepeta pannonica species, the chlorophyll a and b content decreased visibly during the flowering, due to stress. Leaves of plants from this species presented a intense green color in the growing phenophase, while during flowering phenophase they had a purple or yellow coloration. Obtained results revealed a different dynamics of chlorophyll content at studied species.

Interactions between Photosynthetic Pigments Bound to Lipid and Protein Particles. Spectroscopic Properties

1979 ◽  
Vol 34 (7-8) ◽  
pp. 582-587
Author(s):  
Framçoise Techy ◽  
Monique Dinant ◽  
Jacques Aghion
Keyword(s):  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Relative Concentration ◽  
Spectroscopic Properties ◽  
Chlorophyll B ◽  
Pheophytin A ◽  
Chlorophylls A And B ◽  
Protein Particles ◽  
Β Carotene

Abstract The spectroscopic (visible) properties of pigment-bearing lipid and protein particles extract­ ed from milk show that: 1) chlorophylls a and b bound to separate particles can form aggregates provided their relative concentration is high enough. Neither pheophytin a nor β-carotene, in the same conditions, form observable aggregates. 2) Chlorophylls a and b can co-aggregate when they are bound to the same particles. Pheophytin a as well as β-carotene seem to prevent the aggregation of chlorophyll a. β-carotene has no effect on the aggregation of chlorophyll b.

scholarly journals In Vitro Enzymatic Activity Assays Implicate the Existence of the Chlorophyll Cycle in Chlorophyll b-Containing Cyanobacteria

2019 ◽  
Vol 60 (12) ◽  
pp. 2672-2683 ◽  
Author(s):  
HyunSeok Lim ◽  
Ayumi Tanaka ◽  
Ryouichi Tanaka ◽  
Hisashi Ito
Keyword(s):  
Enzymatic Activity ◽  
Chlorophyll A ◽  
Developmental Stages ◽  
Enzymatic Activities ◽  
Light Acclimation ◽  
Chlorophyll B ◽  
Light Conditions ◽  
Chl A ◽  
Shallow Seas

Abstract In plants, chlorophyll (Chl) a and b are interconvertible by the action of three enzymes—chlorophyllide a oxygenase, Chl b reductase (CBR) and 7-hydroxymethyl chlorophyll a reductase (HCAR). These reactions are collectively referred to as the Chl cycle. In plants, this cyclic pathway ubiquitously exists and plays essential roles in acclimation to different light conditions at various developmental stages. By contrast, only a limited number of cyanobacteria species produce Chl b, and these include Prochlorococcus, Prochloron, Prochlorothrix and Acaryochloris. In this study, we investigated a possible existence of the Chl cycle in Chl b synthesizing cyanobacteria by testing in vitro enzymatic activities of CBR and HCAR homologs from Prochlorothrix hollandica and Acaryochloris RCC1774. All of these proteins show respective CBR and HCAR activity in vitro, indicating that both cyanobacteria possess the potential to complete the Chl cycle. It is also found that CBR and HCAR orthologs are distributed only in the Chl b-containing cyanobacteria that habitat shallow seas or freshwater, where light conditions change dynamically, whereas they are not found in Prochlorococcus species that usually habitat environments with fixed lighting. Taken together, our results implicate a possibility that the Chl cycle functions for light acclimation in Chl b-containing cyanobacteria.

scholarly journals PHOTOSYNTHETIC PIGMENT CONTENT AS A CONSEQUENTAL RESISTANCE SIGN OF POTATO VARIETIES TO HIGH TEMPERATURE AND MOISTURE LACK

2020 ◽  
Vol 5 (2) ◽  
pp. 8-13
Author(s):  
Aleksei Bakunov ◽  
Aleksei Milekhin ◽  
Sergei Rubtsov ◽  
Sergei Shevchenko
Keyword(s):  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Photosynthetic Pigment ◽  
Negative Relationship ◽  
Average Concentration ◽  
Potato Yield ◽  
Chlorophyll B ◽  
High Concentration ◽  
Middle Volga Region ◽  
Plant Nursery

The aim of the research is increasing potato yield in dry conditions of the Middle Volga region. The research was carried out on the experimental field of the Samara Scientific Research Institute of Agriculture – branch of the Sa-mara Research Center of the Russian Academy of Sciences. The variety testing plant nursery included 28 varieties of potatoes of domestic selection. Udacha, Zhigulevsky, Arosa and Gala breeds were the standard varieties. The plant material was planted out in two replications with 25 plants in each. To determine the content of photosynthetic pigments, extracts were prepared from ten potato leaves of each breed in 100% acetone. Measurements were per-formed by spectrophotometry. To measure the amount of chlorophyll a, the wavelength of 665 nm, chlorophyll b – 649 nm, and carotenoids – 440 nm were used. The concentration of pigments was determined by using Wettstein formulas. When harvesting, the potato yield was taken into account. The average concentration of chlorophyll a in the studied breeds was 0.92 mg/g, the average concentration of chlorophyll b was 0.62 mg/g, and one of carote-noids was 0.27 mg/g. Potato breeds with high concentration of photosynthetic pigments were identified. A reliable average dependence of the yield of potato breeds on the concentration of chlorophyll b in plants was revealed. The correlation coefficient was 0.42. There is no reliable dependence tested of yield on the concentration of chlorophyll a and carotenoids. Potato productivity is associated with a significant negative relationship with the ratio of both a and b chlorophyll concentrations. The most highly productive varieties were characterized by a high content of two varieties of chlorophyll. A high concentration of chlorophyll b or a minimum ratio of chlorophyll a and b concentra-tions can be tentatively recommended as a consequential sign for identifying potato breeds that are highly adapted to high air temperature and insufficient moisture.

scholarly journals EDTA and nitric acid responses on nickel uptake, translocation factor and pigments on Spinacia oleracea L. replanted seedlings in hydroponic solution

2021 ◽  
Vol 25 (9) ◽  
pp. 1575-1580
Author(s):  
S. Abdulsalam ◽  
M.K. Yahaya ◽  
L.O. Habib ◽  
N.O. Ugbenyo
Keyword(s):  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Antioxidant Defense ◽  
Spinacia Oleracea ◽  
Translocation Factor ◽  
Antioxidant Defense System ◽  
Chlorophyll B ◽  
Defense System ◽  
Spinacia Oleracea L ◽  
Nickel Uptake

The effects of Na2EDTA and HNO3 on Ni2+ uptake by Spinacia oleracea seedlings replanted inhydroponic culture in a greenhouse was investigated. Eight week old seedlings, were exposed to various doses of Ni2+ (0, 1000, 2000, and 4000 mg/L) as NiSO4, at (0, 500 and 3000 mg/L) Na2EDTA and ( 0, 500 and 3000 mg /L) HNO3 in different combinations. There was a substantial increase in nickel uptake in chelated treatments (p < 0.05) compared to unchelated treatments of same concentrations of Ni2+. So, chelation enhanced Ni2+ uptake in S. oleracea. During the exposure, antioxidant defense system helped the plant to protect itself from the damage. Due to increasing nickel  uptake by the plant, the photosynthetic pigments (i.e chlorophyll a, chlorophyll b and Caretenoids) gradually declined. In this study, Spinacia oleracea Seedlings and contents of the photosynthetic pigments (chlorophyll a, chlorophyll b and Caretenoids) of both chelated and unchelated hydroponic treatments were investigated. Changes in photosynthetic pigments was significant (p < 0.05) with respect to addition of EDTA and HNO3 at different concentration to different concentrations of Ni2+ compared to unchelated treatments of same concentrations of Ni2+. The Ni2+ induced translocation factor was also determined which increased significantly (P < 0.05) with increasing Ni2+ concentrations.

A warm white light macromolecular complex phosphor containing Eu(III),Tb(III) and Zn(II) with low color temperature and high color index

2021 ◽  
Vol 134 ◽  
pp. 106636
Author(s):  
Hui Zhao ◽  
Yongchao Wang ◽  
Qian Wang ◽  
Jiandong Guo ◽  
Aiqin Zhang ◽  
...  
Keyword(s):  
White Light ◽  
Color Index ◽  
Color Temperature ◽  
Macromolecular Complex ◽  
Warm White Light

scholarly journals The Qualitative Differences for Photosynthetic Content of Common Bean (Phaseolus vulgaris L.) Populations  in Kosovo

2014 ◽  
Vol 6 (3) ◽  
pp. 331-334
Author(s):  
Sali Ali ALIU ◽  
Imer RUSINOVCI ◽  
Shukri FETAHU ◽  
Bekim GASHI
Keyword(s):  
Common Bean ◽  
Chlorophyll A ◽  
Room Temperature ◽  
Genetic Material ◽  
Carotenoid Content ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Breeding Programs ◽  
Chl A ◽  
Acetone Water

Genetic diversity analysis of common bean populations is useful for breeding programs, as it helps to select genetic material to be used for further crossings. Twenty (20) common bean populations were analyzed using qualitative traits, chlorophyll “a” (Chl ‘a’), chlorophyll “b” (Chl ‘b’), total chlorophyll “a+b” (Total Chl) and carotenoides. The design of the experiment was conducted with leaves of common bean collected from different regions of Kosovo. The experiment was completely randomly with four repetitions. Pigments were extracted by grinding 80-100 mg freshly sampled leaves in 80% (v/v) acetone/water containing MgCO3, at room temperature, preserved in the dark for 24 hours. Concentration of chlorophyll and carotenoid content was measured by spectrophotometer using absorbance recorded at 663 nm, 644 nm and 452.3 nm for maximum absorption of Chl ‘a’, Chl ‘b’, and carotenoids respectively. According to our data the differences between populations for Chl ‘a’, and Chl ‘b’ was significantly higher at level of probability LSDp=0.01. The average values for Chl ‘a’, was 1.67 mg.g-1, while for Chl‘b’was 0.74 mg.g-1. In addition, the results for carotenoids content between populations were with high differences.

scholarly journals Short-term physiological responses to copper stress in Salvinia auriculata Aubl.

2014 ◽  
Vol 26 (3) ◽  
pp. 268-277 ◽  
Author(s):  
Andresa Lana Thomé Bizzo ◽  
Aline Chaves Intorne ◽  
Pollyana Honório Gomes ◽  
Marina Satika Suzuki ◽  
Bruno dos Santos Esteves
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Electrolyte Leakage ◽  
Physiological Responses ◽  
Reactive Oxygen ◽  
Copper Stress ◽  
Oxygen Species ◽  
Chlorophyll B

AIM: To evaluate, in a short-time exposure, the physiological responses of Salvinia auriculata Aubl. under different concentrations of Cu. METHODS: The plants were exposed to treatments with 0.01, 0.1, 1 and 10 mM of Cu in a period of 2 days. Then development variables of S. auriculata (weight, photosynthetic pigments, and soluble carbohydrate), lipid peroxidation (malondialdehyde, aldehydes, and electrolyte leakage) and production of antioxidants (anthocyanins, carotenoids, flavonoids, and proline) were evaluated. RESULTS: It was observed fresh weight reductions in concentrations above 1 mM of Cu. Chlorophyll a decreased with the increase of Cu concentrations unlike chlorophyll b. The ratio chlorophyll a / chlorophyll b was changed due to the degradation of photosynthetic pigments. The reductions of carotenoids were more pronounced than that of total chlorophyll. The values of electrolyte leakage ranged from 14 to 82 % and lipid peroxidation from 7 to 46 nmol.g-1. Flavonoids and soluble carbohydrates showed reductions with the increase of Cu concentration. Anthocyanins, phenolic compounds, and proline when subjected to 0.1 mM of Cu had increased, suggesting adaptability of plant stress caused directly by metal and reactive oxygen species. In higher concentrations, degradation and/or direct modifications of these molecules possibly occurred. CONCLUSIONS: The data suggest that S. auriculata is provided with an efficient mechanism against stress caused by Cu in the concentration of 0.1 mM. As for higher concentrations (1 and 10 mM), despite its role as micronutrients, Cu was toxic to the plant due to the redox behavior of this metal, which leads to the exacerbated formation of reactive oxygen species, inducing to severe damage such as biological membrane degradation and protein denaturation.

scholarly journals The balance of photosynthetic pigments pools and phenolic compounds in dwarf shrubs on the weakly disrupted territory

2019 ◽  
Vol 8 (3) ◽  
pp. 84-89
Author(s):  
Marina Vyacheslavovna Ustinova ◽  
Inessa Vyacheslavovna Kravchenko ◽  
Svetlana Nikolaevna Rusak ◽  
Diana Athamzhanovna Yadgarova
Keyword(s):  
Phenolic Compounds ◽  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Vaccinium Myrtillus ◽  
Chlorophyll B ◽  
Direct Average ◽  
Biochemical Assessment ◽  
B 31 ◽  
Vaccinium Myrtillus L ◽  
Khanty Mansi Autonomous

The paper identifies photosynthetic pigments pools balance and phenolic compounds in suffruticous species of Vaccinium myrtillus L. and Vaccinium vitis-idaea L. on the Tundrinsky bor territory (Khanty-Mansi Autonomous Okrug - Yugra) for the purpose of ecological and biochemical assessment of the valuable plant species status. The authors carry out a comparative analysis of the studied biochemical parameters (chlorophyll a , chlorophyll b , carotenoids and phenolic compounds) of two species leaves, collected from two sample plots similar in landscape and soil and hydrological conditions of the weakly disrupted territory of the Tundrinsky bor territory. The analysis of the suffruticous species leaves for chlorophyll content revealed the following distribution of chlorophyll pools: chlorophyll a - 64,96%, chlorophyll b - 35,04% for leaves of Vaccinium myrtillus and chlorophyll a - 68,28%, chlorophyll b - 31, 72% for leaves of Vaccinium vitis-idaea . The average content of carotenoids and phenolic compounds of Vaccinium myrtillus leaves was 0,62 0,11 mg/g and 14,18 1,65 mg/g, respectively, in Vaccinium vitis-idaea leaves - 0,52 0,12 mg/g and 18,79 2,25 mg/g, respectively. The correlation analysis showed the presence of a direct average strength correlation (r = 0,4) between the levels of chlorophyll and phenolic compounds in the leaves of Vaccinium vitis-idaea .

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