Antioxidant Activities of n-Butanol and Ethyl Acetate Extracts from Yam (Dioscorea batatas DECNE.)

2015 ◽  
Vol 32 (4) ◽  
pp. 599-606 ◽  
Author(s):  
Yishan Duan ◽  
Gyeong-Hwuii Kim ◽  
Jong-Hwan Seong ◽  
Hun-Sik Chung ◽  
Han-Soo Kim
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Dioscorea Batatas ◽  
Ethyl Acetate Extracts

In Vitro Antioxidant Activity Metaplexis Japonica Makino Extract and Derivatives

2014 ◽  
Vol 955-959 ◽  
pp. 387-389 ◽  
Author(s):  
Bao Qing Wang
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Acid Test ◽  
Acetone Extracts ◽  
Β Carotene ◽  
Ethyl Acetate Extracts

Antioxidant activities of acetone and ethyl acetate extracts from Metaplexis japonica Makino, one of famous medicine plants in the eastnorth region of China, named luomo in Chinese, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, ethyl acetate extracts and derivative were IC50 were 313.21, 266.92 and 118.78μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 285.09, 351.57 and 123.89μg/mL. It was concluded that Metaplexis japonica Makino and its derivatives might be a potential natural source of antioxidants .

scholarly journals Fatty acid analysis, cytotoxicity, antimicrobial and antioxidant activities of different extracts of the flowers of Nyctanthes arbor-tristis L.

2020 ◽  
Vol 55 (3) ◽  
pp. 207-214
Author(s):  
MM Haque ◽  
N Sultana ◽  
SMT Abedin ◽  
N Hossain ◽  
SE Kabir
Keyword(s):  
Antibacterial Activity ◽  
Fatty Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Fatty Acid Analysis ◽  
Cytotoxicity Test ◽  
Methanol Extracts ◽  
Vincristine Sulphate ◽  
Ic50 Values ◽  
Ethyl Acetate Extracts

The fatty acid analysis and biological activity of n-hexane, dichloromethane, ethyl acetate and methanol extracts of Nyctanthes arbor-tristis L. flowers are reported. Five fatty acids namely palmitic (44.15%), stearic (19.34%), arachidic (15.06%), behenic (9.77%) and lignoceric (11.69%) acids were identified. From cytotoxicity test, the LC50 values (the median lethal concentration) for n-hexane, dichloromethane, ethyl acetate and methanol extracts as well as for standard vincristine sulphate were found 7.05, 4.67, 3.14, 5.53 and 0.50 μg/ml, respectively. Antibacterial activity results of different extracts were compared with standard antibiotic ciprofloxacin and tetracycline by measuring the zone of inhibition diameter in millimeter. The dichloromethane and ethyl acetate extracts showed significant antibacterial activity. From antioxidant activity test, IC50 values (50% inhibitory concentration) of n-hexane, dichloromethane, ethyl acetate, methanol extracts and ascorbic acid were found to be 291.92 mg/ml, 45.74 μg/ml, 21.86 μg/ml, 64.30 μg/ml and 3.98 μg/ml, respectively. Bangladesh J. Sci. Ind. Res.55(3), 207-214, 2020

scholarly journals Free radical-scavenging capacity, antioxidant activity and phenolic content of Pouzolzia zeylanica

2011 ◽  
Vol 76 (5) ◽  
pp. 709-717 ◽  
Author(s):  
Peiyuan Li ◽  
Lini Huo ◽  
Wei Su ◽  
Rumei Lu ◽  
Chaocheng Deng ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Soxhlet Extraction ◽  
Butylated Hydroxytoluene ◽  
Total Phenolic ◽  
In Vitro Test ◽  
Ethyl Acetate Extracts

Pouzolzia zeylanica was extracted with different solvents (acetone, ethyl acetate and petroleum ether), using different protocols (cold-extraction and Soxhlet extraction). To evaluate the antiradical and antioxidant abilities of the extracts, four in vitro test systems were employed, i.e., DPPH, ABTS and hydroxyl radical scavenging assays and a reducing power assay. All extracts exhibited outstanding antioxidant activities that were superior to that of butylated hydroxytoluene. The ethyl acetate extracts exhibited the most significant antioxidant activities, and cold-extraction under stirring seemed to be the more efficacious method for acquiring the predominant antioxidants. Furthermore, the antioxidant activities and total phenolic (TP) content of different extracts followed the same order, i.e., there is a good correlation between antioxidant activities and TP content. The results showed that these extracts, especially the ethyl acetate extracts, could be considered as natural antioxidants and may be useful for curing diseases arising from oxidative deterioration.

scholarly journals Medicinal Properties Screening of Mallotus paniculatus Extract

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
NA Bahaman ◽  
R Ahmad Raus ◽  
Y Ahmad Nor ◽  
AA Mamun ◽  
NS Adzahar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Rural Areas ◽  
Antioxidant Activities ◽  
Butylated Hydroxytoluene ◽  
Anticancer Properties ◽  
Antifungal Properties ◽  
Hela Cell Lines ◽  
Medicinal Properties ◽  
Ethyl Acetate Extracts

Introduction: Traditionally, Mallotus paniculatus (Balik Angin) plant is used in the treatment of various diseases in rural areas such as remedy after childbirth, wound healing and fever. In this present study, four medicinal properties of the plant were investigated which included antibacterial, antifungal, anticancer and antioxidant activities. Materials and Methods: Potential medicinal compounds were extracted from the plant leaves by sonication with 3 different solvents namely ethanol, ethyl acetate and hexane respectively. The antibacterial and antifungal properties were determined using disc diffusion agar and broth dilution assay, the antioxidant activity by DPPH scavenging assay and the anticancer effect by MTT assay. Results: From the screening of the medicinal properties, M. paniculatus leave extracts were shown to possess antibacterial, antioxidant and anticancer properties but not antifungal properties. Ethanolic and ethyl acetate extracts of the leave were active against gram positive bacteria (Staphylococcus aureus and Bacillus subtilis) but not gram negative bacteria (Pseudomonas aeruginosa and Escherichia coli). The antioxidant activity of the ethanolic crude extract was high; with IC50 of 30 μg/ml comparable with the positive controls; ascorbic acid and butylated hydroxytoluene (BHT). Both ethanolic and ethyl acetate extracts were cytotoxic against breast cancer (MCF7), colon cancer (HT-29), cervix cancer (Hela) cell lines. Conclusion: M. paniculatus leave extract has many potential medicinal values for further studies.

scholarly journals Biologically Active Terpenoids fromTamarix Species

2013 ◽  
Vol 15 (3) ◽  
pp. 219 ◽  
Author(s):  
N.А. Sultanova ◽  
Zh.А. Abilov ◽  
А.K. Umbetova ◽  
M.I. Choudhary
Keyword(s):  
Enzyme Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Biologically Active ◽  
Bioactive Substances ◽  
Aerial Parts ◽  
Ethanol Extracts ◽  
Ethyl Acetate Extracts

<p>We carried out research of bioactive substances obtained from ethanol extract (70%). This ethanol extract (70%) was obtained by us from the aerial parts of <em>Tamarix </em>plants (Т.<em>laxa</em> Willd., T.<em>hispida</em> Willd., T.<em>ramosissima</em> Ledeb. and Т.<em>е</em><em>longatа</em> Ledeb.). These plants belong to the family of Tamaricaceae which grow on the territory of Kazakhstan. For the preliminary separation of bioactive substances, the fractional extraction of ethanol extracts (70%) of Tamarix species was carried out using chloroform and ethyl acetate. Chloroform and ethyl acetate extracts contained terpenoids (positive reactions with Lieberman-Burchard reagent and cerium sulfate). Isolation of terpenoids from chloroform and ethyl acetate extracts was achieved by a series of separations on chromatography column over silica gel and these terpenoids were eluted with gradient mixture of hexaneethylacetate (0-100%). From the aerial parts of <em>Tamarix</em> (Т.<em>laxa</em> Willd., T.<em>hispida</em> Willd., T.<em>ramosissima</em> Ledeb., Т.<em>е</em><em>longatа</em> Ledeb) five terpenoids were isolated. Their structures of 1-5 were found using alkaline hydrolysis and UV, IR, <sup>1</sup>H and <sup>13</sup>C NMR, COSY-45°, HMQC, HMBC, EIMS, FABMS (-ve) spectral analyses. Pentacyclic triterpenoids: ursolic acid (1), 3β-al-D- friedoolean-14-en-28-oic acid methyl ether (2), 3-α-[3´´,4´´-dihydroxy-trans-cinnamoyloxy]-D-friedoolean-14-en-28-oic acids (isotamarixen) (3), 3-α-hydroxy-D-friedoolean-14-en-28-oic acid (4) and 3-α-[4´´-dihydroxy-trans-cinnamoyloxy]-Dfriedoolean -14-en-28-oic acid (5) were isolated from the aerial parts of <em>Tamarix</em>. Ethanol extracts (70%), chloroform, ethylacetate extracts from <em>Tamarix</em> laxa, <em>Tamarix</em> elongata showed antioxidant activity. Antioxidant activities were determined using the DPPH (1, 1-diphenyl-2-picrylhydrazyl radical) scavenging method. Ethanol extracts from <em>Tamarix</em> hispida (50%) were found to contain the triterpenoid: 3-α-[3´´, 4´´-dihydroxy-trans-cinnamoyloxy]-D-friedoolean-14-en-28-oic acids (isotamarixen), which were tested in vitro for their effect on the α-glycosidase enzyme activity. 3-α-[3´´, 4´´-dihydroxy-trans-cinnamoyloxy]-D-friedoolean-14-en-28-oic showed inhibiting effect on the α-glycosidase enzyme activity and was found to be a potent antioxidant.</p>

In vitro antioxidant activities and screening of phytochemicals from methanolic and ethyl acetate extracts of Calligonum comosum L’Her

2016 ◽  
Vol 16 (3) ◽  
pp. 209-215 ◽  
Author(s):  
Abdul J. Cheruth ◽  
Khalfan M. A. Al Naqbi ◽  
A. Abdulwahab A. S. El-Kaabi ◽  
Omar W. Y. Odeh ◽  
Karthishwaran Kandhan ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Ethyl Acetate Extracts

scholarly journals Evaluation of Antioxidant Activities From Ethyl Acetate Fraction of Curry Leaf Using DPPH Method

Elkawnie ◽  
2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Ulil Amna ◽  
Halimatussakdiah Halimatussakdiah ◽  
Furqan Nur Ihsan ◽  
Puji Wahyuningsih
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Ethyl Acetate Fraction ◽  
Murraya Koenigii ◽  
Chromatography Method ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ethyl Acetate Extracts

Abstract: Curry Plant (Murraya koenigii (Linn.) Spreng) is one of the plants that thrive in Indonesia. Curry contains secondary metabolites of alkaloids and flavonoids that have the potential as antioxidants. Antioxidants can reduce the activity of free radicals that can cause degenerative diseases such as cancer, coronary heart disease, and premature ageing of body cells by donating the protons to free radical compounds. This study aimed to determine the antioxidant activity and identify the active compound class of ethyl acetate fraction from curry leaves. Separation of secondary metabolites of ethyl acetate extracts was carried out by column chromatography method, which obtained 5 different fractions. The antioxidant activity of fractions A, B, C, D, and E was tested using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method with variations concentrations of 25, 50, 100, 200, and 400 ppm, and ascorbic acid was used as a comparison which measured at a wavelength of 517 nm. The results showed that fractions A and E have weak antioxidant activities, while fractions B, C, and D showed moderate antioxidant activities. The highest antioxidant activity was found in fraction B with an IC50 value of 341.38 ppm, where the fraction showed the presence of secondary metabolites of alkaloids and terpenoids.Abstrak: Tanaman kari ((Murraya koenigii (Linn.) Spreng) merupakan salah satu tanaman yang tersebar di Indonesia. Kari mengandung senyawa metabolit sekunder alkaloid dan flavonoid yang memiliki potensi sebagai antioksidan. Antioksidan dapat mengurangi aktivitas radikal bebas yang dapat menyebabkan penyakit degeneratif seperti kanker, penyakit jantung coroner dan penuaan sel dengan cara menyumbangkan proton pada senyawa radikal bebas. Tujuan dari penelitian ini adalah untuk menentukan aktivitas antioksidan dan mengidentifikasi kelas senyawa aktif fraksi etil asetat dari daun kari. Pemisahan metabolit sekunder dari ekstrak etil asetat dilakukan menggunakan metode kromatografi kolom dengan menghasilkan 5 fraksi yang berbeda. Aktivitas antioksidan dari fraksi A, B, C ,D ,dan E dilakukan menggunakan metode DPPH (1,1-diphenil-2-pikrilhydrazil) dengan variasi konsentrasi 25, 50, 100, 200, dan 400 ppm dan asam askorbat digunakan sebagai perbandingan yang diukur pada panjang gelombang 517 nm. Hasil menunjukkan bahwa fraksi A dan E memiliki aktivitas antioksidan yang lemah, sedangkan fraksi B, C, dan D menunjukkan aktivitas antioksidan sedang. Aktivitas antioksidan yang paling tinggi ditemukan pada fraksi B dengan IC50 341,38 ppm. Fraksi tersebut menunjukkan adanya kandungan senyawa metabolit sekunder alkaloid dan terpenoid. 

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