scholarly journals Biologically Active Terpenoids fromTamarix Species

2013 ◽  
Vol 15 (3) ◽  
pp. 219 ◽  
Author(s):  
N.А. Sultanova ◽  
Zh.А. Abilov ◽  
А.K. Umbetova ◽  
M.I. Choudhary
Keyword(s):  
Enzyme Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Biologically Active ◽  
Bioactive Substances ◽  
Aerial Parts ◽  
Ethanol Extracts ◽  
Ethyl Acetate Extracts

<p>We carried out research of bioactive substances obtained from ethanol extract (70%). This ethanol extract (70%) was obtained by us from the aerial parts of <em>Tamarix </em>plants (Т.<em>laxa</em> Willd., T.<em>hispida</em> Willd., T.<em>ramosissima</em> Ledeb. and Т.<em>е</em><em>longatа</em> Ledeb.). These plants belong to the family of Tamaricaceae which grow on the territory of Kazakhstan. For the preliminary separation of bioactive substances, the fractional extraction of ethanol extracts (70%) of Tamarix species was carried out using chloroform and ethyl acetate. Chloroform and ethyl acetate extracts contained terpenoids (positive reactions with Lieberman-Burchard reagent and cerium sulfate). Isolation of terpenoids from chloroform and ethyl acetate extracts was achieved by a series of separations on chromatography column over silica gel and these terpenoids were eluted with gradient mixture of hexaneethylacetate (0-100%). From the aerial parts of <em>Tamarix</em> (Т.<em>laxa</em> Willd., T.<em>hispida</em> Willd., T.<em>ramosissima</em> Ledeb., Т.<em>е</em><em>longatа</em> Ledeb) five terpenoids were isolated. Their structures of 1-5 were found using alkaline hydrolysis and UV, IR, <sup>1</sup>H and <sup>13</sup>C NMR, COSY-45°, HMQC, HMBC, EIMS, FABMS (-ve) spectral analyses. Pentacyclic triterpenoids: ursolic acid (1), 3β-al-D- friedoolean-14-en-28-oic acid methyl ether (2), 3-α-[3´´,4´´-dihydroxy-trans-cinnamoyloxy]-D-friedoolean-14-en-28-oic acids (isotamarixen) (3), 3-α-hydroxy-D-friedoolean-14-en-28-oic acid (4) and 3-α-[4´´-dihydroxy-trans-cinnamoyloxy]-Dfriedoolean -14-en-28-oic acid (5) were isolated from the aerial parts of <em>Tamarix</em>. Ethanol extracts (70%), chloroform, ethylacetate extracts from <em>Tamarix</em> laxa, <em>Tamarix</em> elongata showed antioxidant activity. Antioxidant activities were determined using the DPPH (1, 1-diphenyl-2-picrylhydrazyl radical) scavenging method. Ethanol extracts from <em>Tamarix</em> hispida (50%) were found to contain the triterpenoid: 3-α-[3´´, 4´´-dihydroxy-trans-cinnamoyloxy]-D-friedoolean-14-en-28-oic acids (isotamarixen), which were tested in vitro for their effect on the α-glycosidase enzyme activity. 3-α-[3´´, 4´´-dihydroxy-trans-cinnamoyloxy]-D-friedoolean-14-en-28-oic showed inhibiting effect on the α-glycosidase enzyme activity and was found to be a potent antioxidant.</p>

In Vitro Antioxidant Activity Metaplexis Japonica Makino Extract and Derivatives

2014 ◽  
Vol 955-959 ◽  
pp. 387-389 ◽  
Author(s):  
Bao Qing Wang
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Acid Test ◽  
Acetone Extracts ◽  
Β Carotene ◽  
Ethyl Acetate Extracts

Antioxidant activities of acetone and ethyl acetate extracts from Metaplexis japonica Makino, one of famous medicine plants in the eastnorth region of China, named luomo in Chinese, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, ethyl acetate extracts and derivative were IC50 were 313.21, 266.92 and 118.78μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 285.09, 351.57 and 123.89μg/mL. It was concluded that Metaplexis japonica Makino and its derivatives might be a potential natural source of antioxidants .

scholarly journals Antioxidant and antibacterial activity of extracts, fractions and isolated substances from the flowers of Acacia podalyriifolia A. Cunn. ex G. Don

2010 ◽  
Vol 46 (4) ◽  
pp. 715-722 ◽  
Author(s):  
Cláudia Alexandra de Andrade ◽  
João Luiz de Souza Carvalho ◽  
Miriam Machado Cunico ◽  
Ana Luísa Lacava Lordello ◽  
Carmen Etsuko Kataoka Higaskino ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Thiobarbituric Acid ◽  
Ethyl Acetate Fraction ◽  
Total Phenol Content

The extracts and fractions from the flowers of A. podalyriifolia were analyzed previously for antibacterial activity using diffusion in disk, Antioxidant properties were evaluated by determining radical scavenging power (DPPH test) and total phenol content was measured (Folin method). The present study describes the in vitro antibacterial (determining minimum inhibitory concentration) and antioxidant activities (by thiobarbituric acid reactive species - TBARS method) for the ethanol extract, dichloromethane and ethyl acetate fractions and two flavanones (naringenin and 5-β-D-glycosyl-naringenin) isolated from the flowers of Acacia podalyriifolia A. Cunn. ex G. Don. The flavanones naringenin and 5-β-D-glycosyl-naringenin had not previously been obtained from this species. The most effective antibacterial activity was observed in the ethyl acetate fraction (MIC=0.25 mg mL-1 against Staphylococcus aureus ATCC 6538, MIC = 0.125 mg mL-1 against Staphylococcus epidermidis ATCC 12229, MIC=0.5 mg mL-1 against Streptococcus pyogenes ATCC 19615, Klebsiella pneumoniae ATCC 13883 and Proteus mirabilis ATCC 43071). The evaluated samples showed antioxidant activity on the TBARS test, especially for ethanol extract (1000 ppm), which was the most active (29.43% ± 0.65) followed by ethyl acetate fraction (1000 ppm, 24.84% ± 1,28), both demonstrating higher activity than that presented by ascorbic acid (1000 ppm, 21.73% ± 1.77), although lower than the BHT (1000 ppm 35.15% ± 3.42), both reference compounds. Naringenin and 5-β-D-glycosyl-naringenin demonstrated antioxidant action, but only naringenin inhibited the growth of gram-positive and gram-negative bacteria.

scholarly journals IN VITRO ANTI-DIABETIC AND ANTI-OXIDANT ACTIVITIES OF ETHANOL EXTRACT OF TINOSPORA SINENSIS

2017 ◽  
Vol 9 (2) ◽  
pp. 42 ◽  
Author(s):  
Anindita Banerjee ◽  
Bithin Maji ◽  
Sandip Mukherjee ◽  
Kausik Chaudhuri ◽  
Tapan Seal
Keyword(s):  
Plant Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Inhibitory Activities ◽  
Ethanol Extracts

Objective: The aim of the present study was to evaluate the alpha (α)-amylase and alpha (α)-glucosidase inhibitory activities and in vitro antioxidant activities of the 80 % aqueous ethanol extracts of Tinosporasinensis Lour (Merr.).Methods: The 80% aq. ethanol extract of the plant was prepared. The plant extract was examined for its antioxidant activity by using free radical 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging method, ABTS radical scavenging ability, reducing power capacity, estimation of total phenolic content, flavonoid content and flavonol content. Different concentrations (2, 4, 8,10and 15 μg/ml) of the extract was subjected to α-amylase inhibitory and α-glucosidase inhibitory activities and IC50were calculated.Results: The study revealed that the different concentrations of the plant extract possessed a very good amount of total phenolics, flavonoid and flavonol and exhibited potent radical scavenging activity using DPPH and ABTS as a substrate. The ethanol extracts exhibited significant α-amylase and α-glucosidase inhibitory activities with an IC50 value1.093µg and 1.04µg dry extract respectively and well compared with standard acarbose drug.Conclusion: Thus, it could be concluded that due to the presence of antioxidant components the plant extracts could be used for the treatment of hyperglycemia, diabetes and the related condition of oxidative stress. This knowledge will be useful in finding more potent components from the natural resources for the clinical development of antidiabetic therapeutics.

scholarly journals Free radical-scavenging capacity, antioxidant activity and phenolic content of Pouzolzia zeylanica

2011 ◽  
Vol 76 (5) ◽  
pp. 709-717 ◽  
Author(s):  
Peiyuan Li ◽  
Lini Huo ◽  
Wei Su ◽  
Rumei Lu ◽  
Chaocheng Deng ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Soxhlet Extraction ◽  
Butylated Hydroxytoluene ◽  
Total Phenolic ◽  
In Vitro Test ◽  
Ethyl Acetate Extracts

Pouzolzia zeylanica was extracted with different solvents (acetone, ethyl acetate and petroleum ether), using different protocols (cold-extraction and Soxhlet extraction). To evaluate the antiradical and antioxidant abilities of the extracts, four in vitro test systems were employed, i.e., DPPH, ABTS and hydroxyl radical scavenging assays and a reducing power assay. All extracts exhibited outstanding antioxidant activities that were superior to that of butylated hydroxytoluene. The ethyl acetate extracts exhibited the most significant antioxidant activities, and cold-extraction under stirring seemed to be the more efficacious method for acquiring the predominant antioxidants. Furthermore, the antioxidant activities and total phenolic (TP) content of different extracts followed the same order, i.e., there is a good correlation between antioxidant activities and TP content. The results showed that these extracts, especially the ethyl acetate extracts, could be considered as natural antioxidants and may be useful for curing diseases arising from oxidative deterioration.

scholarly journals Evaluation of Antioxidant Potentials and α-Amylase Inhibition of Different Fractions of Labiatae Plants Extracts: As a Model of Antidiabetic Compounds Properties

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Soheila Moein ◽  
Elham Pimoradloo ◽  
Mahmoodreza Moein ◽  
Mahmood Vessal
Keyword(s):  
Ethyl Acetate ◽  
Competitive Inhibition ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethyl Acetate Fraction ◽  
Zataria Multiflora ◽  
Otostegia Persica ◽  
Ethanol Extracts ◽  
Prevention Of Diabetes

In an attempt to identify herbal drugs which may become useful in the prevention of diabetes, antioxidant potentials and α-amylase inhibition by the ethanol extracts of two plants belonging to Lamiaceae family, Otostegia persica and Zataria multiflora, and their different fractions were studied. Also, inhibition of α-amylase by Salvia mirzayanii and its fractions was evaluated. All of the samples exhibited antioxidant activities, among which ethyl acetate fraction of Zataria multiflora (17.21±0.17 mg GAE/g) was found to contain the highest amounts of phenols and the ethyl acetate fraction of Zataria multiflora (218 ± 2.76 mg QUE/g) had the most values of flavonoids. Ethyl acetate fraction of Zataria multiflora (IC50 = 3.05±0.51 μg/ml) was shown to have the most reducing power and the ethyl acetate fraction of Zataria multiflora (IC50 = 32.17±1.82 μg/ml) exhibited the highest DPPH radical scavenging. The ethyl acetate fraction of Otostegia persica (99.39±0.94%) showed the highest α-amylase inhibitory activity which was similar to acarbose used as a standard. Mode of α-amylase inhibition of the most samples was uncompetitive except for ZMC, OPP, OPC, and SMP which presented competitive inhibition. The present findings showed that studied samples may have some compounds with antioxidant and antidiabetic effects.

scholarly journals Antioxidant Activities of n-Hexane Soluble and Insoluble Fraction, Ethyl Acetate Soluble and Insoluble Fraction from Ethanol Extract of Sambung Nyawa Leaf (Gynura procumbens (Lour.) Merr.)

2019 ◽  
Vol 24 (2) ◽  
pp. 91
Author(s):  
Purwantiningsih Purwantiningsih ◽  
Retno Murwanti ◽  
Lukman Hakim
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Soluble Fraction ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Insoluble Fraction ◽  
Ic50 Value ◽  
Antioxidant Potency

There were many plants containing flavonoids which can function as antioxidant. Antioxidant can be used to decrease the mortality rate caused by degenerative and infectious diseases. This study aims to determine the antioxidant potency of n-hexane soluble and insoluble fraction, ethyl acetate soluble and insoluble fraction, and the ethanol extract antioxidant activity of Sambung Nyawa leaf (Gynura procumbens (Lour.) Merr.). The ethanol extract of G. procumbens leaves was obtained by maceration using 70% ethanol then fractionated using hexane and ethyl acetate to obtain the n-hexane soluble fraction, n-hexane insoluble fraction, ethyl acetate soluble fraction, and ethyl acetate insoluble fraction. The antioxidant activity study of ethanol extract, n-hexane soluble, and insoluble fraction, ethyl acetate soluble, and insoluble fraction of G. procumbens was carried out using DPPH (2,2- diphenyl-1-picrylhydrazyl) method to evaluate its free radical scavenging activity with a comparison of vitamin C. The antioxidants activity was expressed as IC50 value. The IC50 value for vitamin C and ethanol extract of G. procumbens leaves were 2.41 µg/ml and 200 µg/ml. On the other hand, the n-hexane soluble and insoluble fraction had an IC50 value of 78 µg/ml and 34 µg/ml, while ethyl acetate soluble and insoluble fraction had the potency as an antioxidant with an IC50 value of 419 µg/ml and 151 μg/ml, respectively. Based on the results obtained, the antioxidant potency of G. procumbens ethanol extract and their fraction were successively of n-hexane insoluble fraction > n-hexane soluble fraction > ethyl acetate insoluble fraction > ethanol extract > ethyl acetate soluble fraction.

scholarly journals AKTIVITAS ANTIBAKTERI DAN KOMPONEN KIMIA EKSTRAK HEKSAN, ETIL ASETAT DAN ETANOL BATANG BROTOWALI (Tinospora crispa Linn) ASAL MANOKWARI

2020 ◽  
Vol 16 (2) ◽  
pp. 112-119
Author(s):  
Bimo Budi Santoso ◽  
Argina Argina ◽  
Alfhons D. Sirampun
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Component Analysis ◽  
Chemical Component ◽  
E Coli ◽  
Activity Test ◽  
Ethanol Extracts ◽  
Main Components ◽  
Ethyl Acetate Extracts

Antibacterial activity test and chemical component analysis of hexane, ethyl acetate and ethanol extract of Brotowali stem from Manokwari Regency against B. subtillis and E. coli have been carried out. The extraction of the T. crispa stems was carried out by the maceration method in stages based on the polarity of the solvent. Chemical component analysis was performed using GC-MS and phytochemical tests. Based on the identification of chemical compounds by phytochemical screening, it shows the presence of flavonoids in the hexane extract, while in the ethyl acetate and ethanol extracts there are alkaloids, flavonoids, saponins, and tannins. The results of the chemical component analysis of hexane, ethyl acetate and ethanol extracts with GC-MS showed that the hexane extract contained 55 chemical components with 11 main components, ethyl acetate extract consisted of 39 components with 8 main components and ethanol extract there were 57 components with 8 main components. The results of the antibacterial activity test using the well method showed that the hexane and ethyl acetate extracts showed better antibacterial activity than the ethanol extract against the two types of tested bacteria, namely E. coli and B. subtillis. The antibacterial activity of hexane and ethyl acetate extracts against E. coli was 11.00 and 14.00 mm, respectively and against B. subtillis were 7.00 and 7.50 mm, respectively.

scholarly journals Antileishmanial and cytotoxic activities of four Andean plant extracts from Colombia

2020 ◽  
Vol 13 (10) ◽  
pp. 2178-2182
Author(s):  
Wilson Cardona-G ◽  
Sara Robledo ◽  
Fernando Alzate ◽  
Andrés F. Yepes ◽  
Cristian Hernandez ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Plant Extracts ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Cytotoxic Activities ◽  
Moderate Activity ◽  
Leishmania Panamensis ◽  
Ethanol Extracts ◽  
Ethyl Acetate Extracts

Background and Aim: Licania salicifolia (L.S) Cuatrec., Persea ferruginea (P.F) Kunth, Oreopanax floribundus (O.F), and Psychotria buchtienii (P.B) belong to the families Chrysobalanaceae, Lauraceae, Araliaceae, and Rubiaceae, respectively, which have been used as medicines by communities in the Andes. This study evaluated the leishmanicidal and cytotoxic activities of alcohol and non-alcohol extracts from four Andean plant extracts (L.S, O.F, P.F, and P.B). Materials and Methods: Extracts were obtained by percolation with solvents of different polarities – hexane, dichloromethane, ethyl acetate, and ethanol. Phytochemical screening was conducted based on reported methods. All products were evaluated in vitro to determine the leishmanicidal activity against amastigotes of Leishmania panamensis and cytotoxicity against U937 cells. Results: Flavonoids, triterpenes, and tannins were the main secondary metabolites found. From the results, dichloromethane extracts from O.F and P.B, ethanol extract from P.B, and ethyl acetate extracts of all plants were active, with EC50 <30 μg/mL. Ethyl acetate was the most active extract, which showed EC50 values of 9.8, 14.1, 23.7, and 25.5 μg/mL, for L.S, P.B, O.F, and P.F, respectively. Hexane extracts from P.B and O.F exhibited moderate activity with EC50 values of 84.8 and 87.4 μg/mL, respectively. Hexane and ethanol extracts from O.F, ethyl acetate, and ethanol extracts from L.S, and all extracts from P.F were not toxic. Alternatively, hexane and dichloromethane extracts from L.S and P.B as well as dichloromethane and ethyl acetate extracts from O.F displayed high toxicity. Conclusion: Based on the activity we observed, ethyl acetate extract can continue in its usage in the search for new antileishmanial drugs, mainly ethyl acetate extract from L.S showed activity comparable to meglumine antimoniate and was not cytotoxic.

scholarly journals Ethyl Acetate Fractions of Papaver rhoeas L. and Papaver nudicaule L. Exert Antioxidant and Anti-Inflammatory Activities

Antioxidants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1895
Author(s):  
Hail Kim ◽  
Sanghee Han ◽  
Kwangho Song ◽  
Min Young Lee ◽  
BeumJin Park ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Papaver Rhoeas ◽  
Anti Inflammatory ◽  
Ethanol Extracts ◽  
Pharmacologically Active ◽  
Pharmacologically Active Compounds ◽  
And Oxidative Stress

Abnormal inflammation and oxidative stress are involved in various diseases. Papaver rhoeas L. possesses various pharmacological activities, and a previously reported analysis of the anti-inflammatory effect of P. nudicaule ethanol extracts and alkaloid profiles of the plants suggest isoquinoline alkaloids as potential pharmacologically active compounds. Here, we investigated anti-inflammatory and antioxidant activities of ethyl acetate (EtOAc) fractions of P. nudicaule and P. rhoeas extracts in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. EtOAc fractions of P. nudicaule and P. rhoeas compared to their ethanol extracts showed less toxicity but more inhibitory activity against LPS-induced nitric oxide production. Moreover, EtOAc fractions lowered the LPS-induced production of proinflammatory molecules and cytokines and inhibited LPS-activated STAT3 and NF-κB, and additionally showed significant free radical scavenging activity and decreased LPS-induced reactive oxygen species and oxidized glutathione. EtOAc fractions of P. nudicaule increased the expression of HO-1, GCLC, NQO-1, and Nrf2 in LPS-stimulated cells and that of P. rhoeas enhanced NQO-1. Furthermore, metabolomic and biochemometric analyses of ethanol extracts and EtOAc fractions indicated that EtOAc fractions of P. nudicaule and P. rhoeas have potent anti-inflammatory and antioxidant activities, further suggesting that alkaloids in EtOAc fractions are potent active molecules of tested plants.

scholarly journals IN VITRO ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF FRACTIONATED EXTRACTS AND ISOLATION OF BIOACTIVE COMPONENTS FROM BREYNIA VITIS-IDAEA (BURM. F.) C. E. C. FISCHER LEAVES

2018 ◽  
Vol 54 (2C) ◽  
pp. 354
Author(s):  
Nguyễn Thị Lan Hương
Keyword(s):  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Bioactive Components ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Ethyl Acetate Extracts

This study investigated the antioxidant and antibacterial activities of ethyl acetate, nbutanoland ethanol extracts of Breynia vitis-idaea (Burm.f.) C. E. C. Fischer leaves using invitro assays;and isolated bioactive compounds from the fractioned extract which showed the bestproperties by column chromatography. All extracts showed significant radical scavengingactivities and exhibited antibacterial activities against Enterococcus faecalis, Staphylococcusaureus and methicillin-resistant Staphylococcus aureus (MRSA). Ethyl acetate extracts showedthe highest free radical scavenging capacity with the IC50 values of 99.55 and 94.66 μg/ml (inDPPH and ABTS assays, respectively) and exhibited MIC values of 1.5, 1 and 1 mg/ml againstthe three bacteria, respectively. In addition, from ethyl acetate extracts, one pure compound hasbeen obtained and identified as 6-O-benzoylarbutin.

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