Evaluation of Antioxidant Activities From Ethyl Acetate Fraction of Curry Leaf Using DPPH Method

Abstract: Curry Plant (Murraya koenigii (Linn.) Spreng) is one of the plants that thrive in Indonesia. Curry contains secondary metabolites of alkaloids and flavonoids that have the potential as antioxidants. Antioxidants can reduce the activity of free radicals that can cause degenerative diseases such as cancer, coronary heart disease, and premature ageing of body cells by donating the protons to free radical compounds. This study aimed to determine the antioxidant activity and identify the active compound class of ethyl acetate fraction from curry leaves. Separation of secondary metabolites of ethyl acetate extracts was carried out by column chromatography method, which obtained 5 different fractions. The antioxidant activity of fractions A, B, C, D, and E was tested using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method with variations concentrations of 25, 50, 100, 200, and 400 ppm, and ascorbic acid was used as a comparison which measured at a wavelength of 517 nm. The results showed that fractions A and E have weak antioxidant activities, while fractions B, C, and D showed moderate antioxidant activities. The highest antioxidant activity was found in fraction B with an IC50 value of 341.38 ppm, where the fraction showed the presence of secondary metabolites of alkaloids and terpenoids.Abstrak: Tanaman kari ((Murraya koenigii (Linn.) Spreng) merupakan salah satu tanaman yang tersebar di Indonesia. Kari mengandung senyawa metabolit sekunder alkaloid dan flavonoid yang memiliki potensi sebagai antioksidan. Antioksidan dapat mengurangi aktivitas radikal bebas yang dapat menyebabkan penyakit degeneratif seperti kanker, penyakit jantung coroner dan penuaan sel dengan cara menyumbangkan proton pada senyawa radikal bebas. Tujuan dari penelitian ini adalah untuk menentukan aktivitas antioksidan dan mengidentifikasi kelas senyawa aktif fraksi etil asetat dari daun kari. Pemisahan metabolit sekunder dari ekstrak etil asetat dilakukan menggunakan metode kromatografi kolom dengan menghasilkan 5 fraksi yang berbeda. Aktivitas antioksidan dari fraksi A, B, C ,D ,dan E dilakukan menggunakan metode DPPH (1,1-diphenil-2-pikrilhydrazil) dengan variasi konsentrasi 25, 50, 100, 200, dan 400 ppm dan asam askorbat digunakan sebagai perbandingan yang diukur pada panjang gelombang 517 nm. Hasil menunjukkan bahwa fraksi A dan E memiliki aktivitas antioksidan yang lemah, sedangkan fraksi B, C, dan D menunjukkan aktivitas antioksidan sedang. Aktivitas antioksidan yang paling tinggi ditemukan pada fraksi B dengan IC50 341,38 ppm. Fraksi tersebut menunjukkan adanya kandungan senyawa metabolit sekunder alkaloid dan terpenoid.

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Evaluation of Secondary Metabolites and Antioxidant Activity of Water, Ethyl Acetate and Hexane Fractions from the Mangrove Young Leaves Sonneratia alba

Chemical Science International Journal ◽

10.9734/csji/2021/v30i230215 ◽

2021 ◽

pp. 23-32

Author(s):

Verly Dotulong ◽

Djuhria Wonggo ◽

Lita A. D. Y. Montolalu

Keyword(s):

Antioxidant Activity ◽

Secondary Metabolites ◽

Ethyl Acetate ◽

Secondary Metabolite ◽

Antioxidant Activities ◽

Extraction Methods ◽

Water Fraction ◽

Sonneratia Alba ◽

Dpph Method ◽

Young Leaves

The fractions from young leaves of mangrove Sonneratia alba was studied for its associated secondary metabolites and antioxidant activities. The objective of this study was to determine the secondary metabolite components and antioxidant activity of water, ethyl acetate, and hexane fractions of the young leaves of mangrove S. alba. The fraction was obtained from dry powder of young leaf S.alba using continuous fractionation of crude extracts. The crude extract was attained by 2 extraction methods (soxhlet and maceration) and 2 extraction solvents (methanol and ethanol). Secondary metabolites analyses were qualitatively conducted to detect the presence or absence of phenols, flavonoid, tannin, steroid, triterpenoid and alkaloid. Total phenols were measured using Folin Ciocalteau reagents and gallic acid standard curves whereas antioxidant activity were analyzed using DPPH method (1- 1-diphenil-2-picrihydrasil). Results showed that all fractions contained secondary metabolite components tested. The highest rendement was found in the water fraction fromsoxhletation extract with methanol (6.36±0.29%). The total phenol values were found the highest in the ethylacetatefraction from macerated extract with ethanol (352±9.77 mgGAE/g). Stronger antioxidant activity was also found in ethylacetate fraction as indicated by the small value of IC50 DPPH namely the ethylacetate fraction with soxhletation extract with ethanol (3.43±0.25 µg / mL). The results of this study indicate that the semipolar fraction (ethylacetate fraction) has more potential as a source of natural antioxidants.

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IDENTIFICATION OF SECONDARY METABOLIC AND TEST OF ACTIVITY ETHYL ACETATE FRACTION OF BANGUN¬- BANGUN (Coleus amboinicus Lour.) LEAVES AS ANTIOXIDANT

BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) ◽

10.31289/biolink.v7i1.3732 ◽

2020 ◽

Vol 7 (1) ◽

pp. 117-122

Author(s):

Kasta Gurning

Keyword(s):

Antioxidant Activity ◽

Secondary Metabolites ◽

Ethyl Acetate ◽

Oxidation Process ◽

Natural Antioxidants ◽

Ethyl Acetate Fraction ◽

The Body ◽

Synthetic Antioxidants ◽

Dpph Method ◽

Coleus Amboinicus

Antioxidants have an important role in delaying the oxidation process and preventing the occurrence of degenerative diseases in the body. Antioxidants consist of synthetic and natural; the use of synthetic antioxidants in a relatively long period is hazardous so that the use of natural antioxidants is considered more potential and safe for the body. The process of extracting secondary metabolites from the leaves of bangun-bangun (Coleus amboinicus L.) is carried out by the maceration method with methanol, then partitioned in stages with n-hexane, chloroform, and ethyl acetate. This research intends to identify secondary metabolites and test antioxidant activity from the ethyl acetate fraction with the DPPH method. Phytochemical screening results of ethyl acetate fraction showed the different secondary metabolite groups such as alkaloids, phenolics & polyphenols, flavonoids, coumarin, and triterpenoids. Antioxidant activity (IC50) obtained is 64.97 with a healthy category, so that the potential to be used as a natural antioxidant.

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AKTIVITAS ANTIOKSIDAN DAN FLAVONOID EKSTRAK BIJI KURMA

EKOLOGIA ◽

10.33751/ekol.v19i1.1660 ◽

2020 ◽

Vol 19 (1) ◽

pp. 34-38

Author(s):

Siti Warnasih ◽

Diana Widiastuti ◽

Uswatun Hasanah ◽

Laksmi Ambarsari ◽

Purwantiningsih Sugita

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Methanol Extract ◽

Soxhlet Extraction ◽

Ethyl Acetate Fraction ◽

Positive Control ◽

Palm Fruit ◽

Ic50 Value ◽

Dpph Method ◽

Fruit Processing

Date seeds are waste from palm fruit processing that has not been utilized optimally. Date seeds contain flavonoids which are known to act as antioxidants. The aim of this study was to determine the antioxidant activity and flavonoid of methanol extract which was carried out by soxhletation and its fractionation results. Date seeds are made into simplicia, soxhlet extraction with methanol, then the methanol extract is fractionated in stages by liquid-liquid partition with n-hexane, ethyl acetate, and n-butanol. Each fraction and extract was determined by its antioxidant activity with the DPPH method and its flavonoid was determined by spectrophotometry. Ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 5.74 ± 0.05μg/mL, followed by methanol extract of 9.55±0.53 μg/mL, n-butanol fraction 19.73±0,58 μg/mL, and n-hexane fraction of 289.59±10.52 μg/mL, while the IC50 value for vitamin C as a positive control was 4.29±0.74 μg/mL. The highest flavonoids were produced from ethyl acetate fractions which amounted to 1484.33 ± 161.47 mg Quercetin Equivalent (QE) / 100 g, followed respectively by methanol extract of 282.84±13.72 mg QE/100 g.

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Phytochemical Screening and Antioxidant Activity of Date(Phoenix Dactylifera) Seed Extracts

International Journal of Recent Technology and Engineering - 2 ◽

10.35940/ijrte.b1018.0782s719 ◽

2019 ◽

Vol 8 (2S7) ◽

pp. 87-89

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Methanol Extract ◽

Ethyl Acetate Fraction ◽

Hexane Fraction ◽

Phytochemical Screening ◽

Water Fraction ◽

Ic50 Value ◽

Dpph Method

Dates consisting of 90% flesh have been known to have various benefits, but the remaining 10% of them in the form of seeds has not been utilized optimally. The date palm contains a fairly high polyphenol compound, which can act as an antioxidant. This study aims to determine the antioxidant activity of methanol extract and fractionation of the extract of dates using the in vitro method of DPPH (1,1-diphenyl-2-picrilhidrazil). The date seeds are made into simplicia, macerated with methanol, and then the extracted methanol is gradually fractionated by n-hexane, ethyl acetate, n-butanol, and water. Each fraction and extract is phytochemically screened using the standard methods and its antioxidant activity is determined by DPPH method. Methanol extract, ethyl acetate, n-butanol, and water fractions contain tannins, flavonoids, alkaloids, and saponin, while the n-hexane fraction not contain phytochemical compound based on the phytochemical screening. The ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 3.72 ± 0.44 μg/mL, followed by methanol extract of 4.71 ± 0.64 μg/mL, n-butanol fraction of 6.62 ± 0.08 μg/mL, water fraction of 53.00 ± 18.96 μg/mL, and n-hexane fraction of 250.19 ± 9.69 μg/ mL, while the IC50 value of vitamin C as the positive control is 4.29 ± 0.74 μg/mL. Therefore, date seeds can be a source of natural antioxidants

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POTENSI ANTIOKSIDAN METABOLIT SEKUNDER FRAKSI ETIL ASETAT DARI KULIT BATANG EBONI (Diospyros celebica Bakh.)

KOVALEN ◽

10.22487/kovalen.2019.v5.i2.11642 ◽

2019 ◽

Vol 5 (2) ◽

pp. 155-165

Author(s):

Vivi Sasmita ◽

Ahmad Ridhay ◽

Hardi Ys

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Secondary Metabolites ◽

Ethyl Acetate ◽

Spectral Data ◽

Ethyl Acetate Extract ◽

Ethyl Acetate Fraction ◽

Wave Number ◽

Antioxidant Compounds ◽

Ic50 Value

Research on the antioxidant potential of the secondary metabolite compound, ethyl acetate from ebony bark (Diospyros celebica Bakh.) has been conducted. This study aims to characterize and determine the antioxidant activity of secondary metabolites of ethyl acetate fraction from ebony bark. Ethyl acetate extract was fractionated using Liquid Vacum Chromatography (KVC) and Gravity Column Chromatography (KKG). The isolated compounds were characterized using UV-Vis and IR spectrophotometers. Data on maximum absorption of λmax 273 nm from UV-Vis analysis showed the presence of chromophore phenol. IR spectral data showed several specific peaks, among others, at wave number (ʋ) of 3425.58 cm-1 indicating the presence of –OH group, C-H aliphatic bond at ʋ of 2924.09 cm-1 and 2854.65 cm-1, and the peak at ʋ of 1604.77 -1465.90 cm-1 and 3078.39 cm-1 indicated the aromatic group, reinforcing the assumption that the isolates produced were phenolic compounds. IC50 value of 75.47 µg/mL from antioxidant activity testing showed that the isolates obtained were antioxidant compounds with strong antioxidant categories. Keywords: antioxidant, Diospyros, ebony, phenolic, isolation

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Ethanol Extract, Ethyl Acetate Extract, Ethyl Acetate Fraction, and n-Heksan Fraction Mangosteen Peels (Garcinia mangostana L.) As Source of Bioactive Substance Free-Radical Scavengers

JPSCR Journal of Pharmaceutical Science and Clinical Research ◽

10.20961/jpscr.v1i2.1936 ◽

2016 ◽

Vol 1 (2) ◽

pp. 71

Author(s):

Liza Pratiwi ◽

Achmad Fudholi ◽

Ronny Martien ◽

Suwidjiyo Pramono

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

Hexane Fraction ◽

Garcinia Mangostana ◽

Ic50 Value ◽

Dpph Method ◽

Peel Extract

Mangosteen peels (Garcinia mangostana L.) is well known as an excellent source of antioxidative compounds. The name of mangosteen is xanthone. Antioxidant of mangosteen peels can be extracted by ethanol, etyl acetate and can be fractinated by etyl acetate and n-hexane. The aim of this research was to compare the antioxidant activity of the peel extract by ethanol and etyl acetate and fractinated by etyl acetate and n-hexane. Extract and fraction exhibited higher scavenging activity of DPPH. The purpose of this study was to compare antioxidant activity of ethanol extract, ethyl acetate extract, fraction of ethyl acetate and n-hexane fraction. The antioxidant activity test using DPPH method with UV-Vis spectrophotometer. Ethanol extract shown IC50 value 5,03 µg/mL, ethyl acetate extract shown IC50 value 41,56 µg/mL. Ethyl acetate fraction shown IC50 value 2,78 µg/mL, and n-hexane fraction with IC50 22,33 µg/mL. It means peel extract and fraction by mangosteen peels has very strong antioxidant activity and ethyl acetate fraction that its antioxidant activity higher that the other solvent.

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Antioxidant Activity, Inhibition α-Glucosidase of Ethanol Extract of Strychnos nitida G. Don and Identification of Active Compounds

Current Biochemistry ◽

10.29244/cb.5.3.11-20 ◽

2019 ◽

Vol 5 (3) ◽

pp. 11-20

Author(s):

Stefani Dhale Rale ◽

Hasim Hasim ◽

Syamsul Falah

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

Inhibition Activity ◽

Active Compounds ◽

Activity Inhibition ◽

Activity Test ◽

Ic50 Value ◽

Treatment Of Diabetes

This study aims to find the treatment of diabetes using natural materials by exploring plants in the province of East Nusa Tenggara. his research was conducted out by extracting the Strychnos nitida G.Don stem using a method of maceration by ethanol 70%. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Simplicia from maceration and fractionation results were then tested for antioxidant activity, α-glucosidase inhibition activity and identification of active compounds. The results showed that ethyl acetate fraction had the lowest IC50 value of 86.83 μg / ml. Results of the α-glucosidase activity test showed that ethyl acetate fraction and n-heksan fraction at 900 ppm had the highest percentage of inhibition of 34.23% and 33.89%. Identification using LCMS/MS method showed that ethyl acetate fraction consist of Benzenemethamine, N, N-dioctyl- as an antioxidantcompound and compound 24-methyl-5-cholestone-hexol as an antidiabetic compound. From the results of this study, we concluded that the extract of kayu ular Strychnos nitida G.Don stem has inhibition activity toward α-glucosidase enzyme.

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In Vitro Antioxidant Activity Metaplexis Japonica Makino Extract and Derivatives

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.955-959.387 ◽

2014 ◽

Vol 955-959 ◽

pp. 387-389 ◽

Cited By ~ 2

Author(s):

Bao Qing Wang

Keyword(s):

Antioxidant Activity ◽

Linoleic Acid ◽

Ethyl Acetate ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Acid Test ◽

Acetone Extracts ◽

Β Carotene ◽

Ethyl Acetate Extracts

Antioxidant activities of acetone and ethyl acetate extracts from Metaplexis japonica Makino, one of famous medicine plants in the eastnorth region of China, named luomo in Chinese, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, ethyl acetate extracts and derivative were IC50 were 313.21, 266.92 and 118.78μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 285.09, 351.57 and 123.89μg/mL. It was concluded that Metaplexis japonica Makino and its derivatives might be a potential natural source of antioxidants .

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Limpasu Pericarpium : an Alternative Source of Antioxidant From Borneo with Sequential Maceration Method

Jurnal Profesi Medika Jurnal Kedokteran dan Kesehatan ◽

10.33533/jpm.v15i1.2820 ◽

2021 ◽

Vol 15 (1) ◽

Author(s):

Irfan Zamzani ◽

Nita Triadisti

Keyword(s):

Antioxidant Activity ◽

Free Radicals ◽

Ethyl Acetate ◽

Methanol Extract ◽

Oxidation Process ◽

Antioxidant Activities ◽

Alternative Source ◽

Dpph Method ◽

Indigenous Plant ◽

Ic50 Values

Antioxidants are substances that can slow down the oxidation process of free radicals. Limpasu plant (Baccaurea lanceolata (Miq) Muell. Arg), an indigenous plant of Borneo, is a natural antioxidant source. The purpose of this study was to determine the antioxidant activity of the limpasu pericarpium extract. The extraction of the limpasu pericarpium was done by maceration method using solvents with increasing polarity ranging from n-hexane, ethyl acetate, and methanol. Antioxidant activities of the three extracts were measured by the DPPH and FRAP methods. The IC50 values of n-hexane, ethyl acetate, and methanol extracts, as well as quercetin using the DPPH method were 517,45 µg/mL, 530,64 µg/mL, 10,63 µg/mL and 6,83 µg/mL, respectively. Meanwhile, the IC50 values obtained from FRAP method were 198,96 µg/mL, 190,07 µg/mL, 661,36 µg/mL, and 7,09 µg/mL, respectively. The results revealed that the methanol extract is more potent than other extracts tested for antioxidant activity.

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CYTOTOXIC AND ANTIOXIDANT ACTIVITIES OF SECONDARY METABOLITES FROM PULICARIA UNDULATA

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2016v8i9.12814 ◽

2016 ◽

Vol 8 (9) ◽

pp. 150 ◽

Cited By ~ 6

Author(s):

Taha A. Hussien ◽

Sayed A. El-toumy ◽

Hossam M. Hassan ◽

Mona H. Hetta

Keyword(s):

Antioxidant Activity ◽

Secondary Metabolites ◽

Antioxidant Activities ◽

Structure Activity Relationship ◽

Activity Relationship ◽

Hep G2 ◽

Structure Activity ◽

Dpph Method ◽

Mcf 7

Objective: To evaluate the in vitro cytotoxicity, antioxidant activities and structure-activity relationship of secondary metabolites isolated from Pulicaria undulata.Methods: The methylene chloride-methanol (1:1) extract of the air-dried aerial parts of Pulicaria undulata was fractionated and separated to obtain the isolated compounds by different chromatographic techniques. Structures of the isolated compounds were determined on the basis of the extensive spectroscopic analysis, including 1D and 2D NMR and compared with the literature data. The crude extract and the isolated compounds were evaluated for in vitro antioxidant activity using the 2,2 diphenyl dipicryl hydrazine (DPPH) method and cytotoxic assay using human breast cancer (MCF-7) and hepatoma (Hep G2) cell line.Results: Nine secondary metabolites were isolated from Pulicaria undulata in this study. Of which two terpenoidal compounds; 8-epi-ivalbin and 11β, 13-dihydro-4H-xanthalongin 4-O-β-D-glucopyranoside firstly isolated from the genus pulicaria and three flavonoids; eupatolitin, 6-methoxykaempferol, and patulitrin firstly isolated from P. undulata. 6-methoxykaempferol (IC50 2.3 µg/ml) showed the most potent antioxidant activity. The highest cytotoxic effect against MCF-7 and Hep G2 cells was obtained with eupatolitin (IC50 27.6 and 23.5 µg/ml) respectively. The structure-activity relationship was also examined and the findings presented here showed that 3, 5, 7, 4' and 3, 5, 4', 5'-hydroxy flavonoids were potent antioxidant and has cytotoxic activity.Conclusion: Pulicaria undulata is a promising medicinal plant, and our study tends to support the therapeutic value of this plant as antioxidant drug and in the treatment of cancer.

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