scholarly journals Fatty acid analysis, cytotoxicity, antimicrobial and antioxidant activities of different extracts of the flowers of Nyctanthes arbor-tristis L.

2020 ◽  
Vol 55 (3) ◽  
pp. 207-214
Author(s):  
MM Haque ◽  
N Sultana ◽  
SMT Abedin ◽  
N Hossain ◽  
SE Kabir
Keyword(s):  
Antibacterial Activity ◽  
Fatty Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Fatty Acid Analysis ◽  
Cytotoxicity Test ◽  
Methanol Extracts ◽  
Vincristine Sulphate ◽  
Ic50 Values ◽  
Ethyl Acetate Extracts

The fatty acid analysis and biological activity of n-hexane, dichloromethane, ethyl acetate and methanol extracts of Nyctanthes arbor-tristis L. flowers are reported. Five fatty acids namely palmitic (44.15%), stearic (19.34%), arachidic (15.06%), behenic (9.77%) and lignoceric (11.69%) acids were identified. From cytotoxicity test, the LC50 values (the median lethal concentration) for n-hexane, dichloromethane, ethyl acetate and methanol extracts as well as for standard vincristine sulphate were found 7.05, 4.67, 3.14, 5.53 and 0.50 μg/ml, respectively. Antibacterial activity results of different extracts were compared with standard antibiotic ciprofloxacin and tetracycline by measuring the zone of inhibition diameter in millimeter. The dichloromethane and ethyl acetate extracts showed significant antibacterial activity. From antioxidant activity test, IC50 values (50% inhibitory concentration) of n-hexane, dichloromethane, ethyl acetate, methanol extracts and ascorbic acid were found to be 291.92 mg/ml, 45.74 μg/ml, 21.86 μg/ml, 64.30 μg/ml and 3.98 μg/ml, respectively. Bangladesh J. Sci. Ind. Res.55(3), 207-214, 2020

In Vitro Antioxidant Activity Metaplexis Japonica Makino Extract and Derivatives

2014 ◽  
Vol 955-959 ◽  
pp. 387-389 ◽  
Author(s):  
Bao Qing Wang
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Acid Test ◽  
Acetone Extracts ◽  
Β Carotene ◽  
Ethyl Acetate Extracts

Antioxidant activities of acetone and ethyl acetate extracts from Metaplexis japonica Makino, one of famous medicine plants in the eastnorth region of China, named luomo in Chinese, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, ethyl acetate extracts and derivative were IC50 were 313.21, 266.92 and 118.78μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 285.09, 351.57 and 123.89μg/mL. It was concluded that Metaplexis japonica Makino and its derivatives might be a potential natural source of antioxidants .

scholarly journals Limpasu Pericarpium : an Alternative Source of Antioxidant From Borneo with Sequential Maceration Method

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Irfan Zamzani ◽  
Nita Triadisti
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Oxidation Process ◽  
Antioxidant Activities ◽  
Alternative Source ◽  
Dpph Method ◽  
Indigenous Plant ◽  
Ic50 Values

Antioxidants are substances that can slow down the oxidation process of free radicals. Limpasu plant (Baccaurea lanceolata (Miq) Muell. Arg), an indigenous plant of Borneo, is a natural antioxidant source. The purpose of this study was to determine the antioxidant activity of the limpasu pericarpium extract. The extraction of the limpasu pericarpium was done by maceration method using solvents with increasing polarity ranging from n-hexane, ethyl acetate, and methanol. Antioxidant activities of the three extracts were measured by the DPPH and FRAP methods. The IC50 values of n-hexane, ethyl acetate, and methanol extracts, as well as quercetin using the DPPH method were 517,45 µg/mL, 530,64 µg/mL, 10,63 µg/mL and 6,83 µg/mL, respectively. Meanwhile, the IC50 values obtained from FRAP method were 198,96 µg/mL, 190,07 µg/mL, 661,36 µg/mL, and 7,09 µg/mL, respectively. The results revealed that the methanol extract is more potent than other extracts tested for antioxidant activity.

scholarly journals Antibacterial and Antioxidant Activities of Slender Amaranth Weed

2020 ◽  
Vol 38 ◽  
Author(s):  
M. AKBAR ◽  
I.N. SHERAZI ◽  
M.S. IQBAL ◽  
T. KHALIL ◽  
H.M. WAQAS
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Pseudomonas Syringae ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Bacterial Species ◽  
Radical Scavenging ◽  
Root Extract

ABSTRACT: In the present study, antibacterial and antioxidant [1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity] activities of a weed, slender amaranth (Amaranthus viridis L.) were investigated. Extracts of different plant parts were prepared in n-hexane, chloroform and ethyl acetate. Antibacterial activity was measured by using 100 mg mL-1 concentration extracts against 4 deadly phytopathogenic bacterial species viz. Pseudomonas syringae Van Hall, Ralstonia solanacearum Smith, Erwinia carotovora (Jones), Holland and Xanthomonas axonopodis Hasse. In antioxidants assays, 10, 20 and 30 mg mL-1 extracts were used keeping DPPH as control. In these bioassays, ethyl acetate fraction of A. viridis leaf exhibited the best antibacterial and antioxidants activity. Ethyl acetate leaf fraction showed the highest inhibition zone diameter (IZD) where it caused 21 mm IZD against P. syringae and 19 mm IZD against E. carotovora. This extract also showed 22, 52 and 84% antioxidant activity at 10, 20 and 30 mg mL-1 concentrations, respectively. Previously there is no report available that describes antibacterial activity of root extract of A. viridis against P. syringae. Moreover, antioxidant activity of stem and root extracts in n-hexane, chloroform and ethyl acetate was investigated first time in the world. It was concluded that the biological activities observed during the present investigation may be due to the presence of bioactive constituents that can be harnessed as natural antibacterials and antioxidants.

scholarly journals Cotoneaster transcaucasicus Pojark. Meyve Ekstraklarında Biyoaktif Bileşen Miktarlarının ve Antioksidan Aktivitelerinin Belirlenmesi

2021 ◽  
Vol 9 (7) ◽  
pp. 1258-1263
Author(s):  
Fatma Ergün
Keyword(s):  
Methanol Extract ◽  
Antioxidant Activities ◽  
Hexane Extract ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Methanol Extracts ◽  
Ic50 Values ◽  
Phenolic Substances ◽  
Rosaceae Family ◽  
Phenolic And Flavonoid

In this study, the total phenolic and flavonoid contents and antioxidant activities of hexane and methanol extracts of Cotoneaster transcaucasicus Pojark. fruits in the Rosaceae family were investigated. Using the Folin-Ciocaltaeu method, the amount of phenolic substances was determined as 50.34 ± 2.70 mg of GAE/g in methanol extract and 38.06 ± 1.32 mg of GAE/g in hexane extract. The total amount of flavonoids was calculated as 52.01 ± 3.78 mg of QE/g in the methanol extract and 60.54 ± 6.82 mg of QE/g in the hexane extract, using the aluminum nitrate method. Antioxidant activities of C. transcaucasicus hexane and methanol extracts were evaluated by DPPH and FRAP methods. IC50 values of the extracts were calculated as 0.411 mg/mL for hexane extract and 0.237 mg/mL for methanol extract. In addition, it was determined that the reducing antioxidant power of Fe3+-Fe2+ was higher in hexane extract. As a result, it has been observed that the phenolic and flavonoid content and antioxidant activities of C. transcaucasicus fruits are high. This study is the first study in C. transcaucasicus species and it is thought that it will lead the future studies.

scholarly journals Variation in Phytochemical Constituents and Antioxidant Potential of Extracts Derived from Leaves of Sansevieria trifasciata var. Laurentii and Sansevieria trifasciata var. Zeylanica (Asparagaceae)

2020 ◽  
Vol 32 (9) ◽  
pp. 2303-2307
Author(s):  
NURUL HUDA ABDUL WAHAB ◽  
YVONNE SAMUEL ◽  
NORHAYATI YUSUF ◽  
HANIS MOHD YUSOFF
Keyword(s):  
Ethyl Acetate ◽  
Specific Activity ◽  
Guaiacol Peroxidase ◽  
Phytochemical Analysis ◽  
Enzymatic Antioxidant ◽  
Methanol Extracts ◽  
Crude Extracts ◽  
Cat Assay ◽  
Set Up ◽  
Ethyl Acetate Extracts

Sansevieria is an ornamental plant that has many hybrids and varieties make them difficult to distinguish. The most common varieties used for medicinal purposes are Sansevieria trifasciatam which is known for cure of many diseases. However, little attention is given to this plant in proving it medicinal worth and capability as an antioxidant agent. This study was initiated to set up a metabolite classes profile and the potential enzymatic antioxidant of the variations of these plants. Crude extracts of S. trifasciata var. Laurentii and S. trifasciata var. Zeylanica were prepared from their leaves, and solvent used has different polarities. Qualitative phytochemical analysis was carried out using the extracts. Phytochemical screening suggested both of these samples contain carbohydrates in all extracts. It also show that flavonoid was found in hexane and ethyl acetate extracts while did not observed in the methanol extracts for both samples. However, alkaloid, phenol and tannin were positive in all of the methanol extracts except for hexane and ethyl acetate extracts. For the biological activity, all extracts were selected for the determination of enzymatic antioxidant activity test using catalase (CAT) assay and guaiacol peroxidase (gPOD) assay using UV-VIS spectrophotometer. Based on the results, CAT specific activity was the highest in methanol extract of S. trifasciata var. Laurentii (3.15 ± 0.50 units/mg protein) compared to S. trifasciata var. Zeylanica (2.20 ± 0.05 units/mg protein). For gPOD specific activity, ethyl acetate extract of S. trifasciata var. Laurentii shows the highest activity which is 1.46 × 10-2 ± 0.02 units/mg protein compared to the other crude extracts.

scholarly journals COMPARISON OF INHIBITORY ACTIVITY AGAINST THE α-GLUCOSIDASE ENZYMES IN THE EXTRACTS AND FRACTIONS FROM LEAVES OF THE GARCINIA KYDIA ROXBURGH

2017 ◽  
Vol 10 (7) ◽  
pp. 401
Author(s):  
Septiani Martha ◽  
Berna Elya ◽  
Muhammad Hanafi
Keyword(s):  
Ethyl Acetate ◽  
Inhibitory Activity ◽  
Enzymatic Reaction ◽  
Active Fraction ◽  
Pharmacological Activities ◽  
Percent Inhibition ◽  
Ic50 Values ◽  
Microplate Reader ◽  
Ethyl Acetate Extracts

Objective : Garcinia kydia Roxb. is aspecies of the genus Garcinia, is based chemotaxonomic has various bioactive compounds that have been isolated by a variety of pharmacological activities, one of the activities that are being developed that inhibition of         α-glucosidase. However, α-glucosidase inhibitory activity in the extracts and fraction from leaves of the Garcinia kydia Roxb. has not been reported. In this study, seeks to evaluated of α-glucosidase inhibitory activity against extracts and fractions of potentially.Methods : The α-glucosidase inhibitory activity test, conducted by in-vitro using the enzymatic reaction is measured of quantity with a microplate reader and identify the compound from the active fraction with normal-phase thin layer chromatography.Results : The ethyl acetate and methanol extract have the potential to inhibit the α-glucosidase with the percent inhibition at a concentration of 500μg/mL of 83 and 59%, respectively. The active fraction of the ethyl acetate extracts (FEA8) with percent inhibition at concentrations of 100 mg/mL and IC50 values of 80% and 2,79μg/mL, respectively and active fraction of the methanol extracts (FMT3) with percent inhibition at concentrations of 100 mg/mL and IC50 values of 71% and 8,43 μg/mL, respectively.Conclusion: Garcinia kydia Roxb. evident has the potential to inhibit the α-glucosidase. Flavonoid and phenolic compounds that suspected of acts as α-glucosidase inhibitory activity. Thus, the research will continue the process of isolating the active compound so that it can be developed as natural therapeutic agents in the control of glucose.

Antioxidant Activities of n-Butanol and Ethyl Acetate Extracts from Yam (Dioscorea batatas DECNE.)

2015 ◽  
Vol 32 (4) ◽  
pp. 599-606 ◽  
Author(s):  
Yishan Duan ◽  
Gyeong-Hwuii Kim ◽  
Jong-Hwan Seong ◽  
Hun-Sik Chung ◽  
Han-Soo Kim
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Dioscorea Batatas ◽  
Ethyl Acetate Extracts

scholarly journals THE ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL SCREENING OF ETHANOL EXTRACT, FRACTIONS OF WATER, ETHYL ACETATE AND n-HEXANE FROM MISTLETOE TEA (SCURRULA ATROPURPUREABL. DANS)

2017 ◽  
Vol 10 (2) ◽  
pp. 343 ◽  
Author(s):  
Resmi Mustarichie
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Water Fraction ◽  
Ic50 Values

Objective: The aim of this study is to investigate antioxidant activity and phytochemical screening of ethanol extract, fractions of water, ethyl acetate, and n-hexane from mistletoe tea (Scurrula atropurpurea Bl. Dans).Methods: Simplicia extracted using soxhlet equipment with 96% ethanol. Fractionation was conducted using liquid-liquid extraction using a solvent of water, ethyl acetate and n-hexane. Screening of phytochemical and antioxidant activity was performed against these fractions. Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl method using ultraviolet-visible spectrophotometry with ascorbic acid as standard. Phytochemical screening was conducted based on the method of Farnsworth.Results: The IC50 values of ethanol extract, water fraction, fraction of ethyl acetate, and n-hexane fraction were 21.92 ppm, 89.57 ppm, 14.08 ppm, and 162.09 ppm, respectively, whereas for ascorbic acid was 4.41 ppm. The ethanol extract and ethyl acetate fraction contained compounds were the same group, polyphenolic, tannins, flavonoids, monoterpenoid, steroids, triterpenoids, and quinones. Fraction of water contained compounds such as polyphenolic group, flavonoids, monoterpenoids, sesquiterpenoids, steroids, and triterpenoids. n-hexane fraction compounds contained steroids and triterpenoids.Conclusion: The ethanol extract, water fraction, ethyl acetate fraction, and n-hexane fraction showed antioxidant activities. The ethanol extract, fractions of water, and ethyl acetate fraction contained flavonoids and polyphenolic potential as antioxidants.Keywords: Mistletoe tea, Scurrula atropurpurea, Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Ascorbic acid.

scholarly journals The Measurement of Antioxidant Activity of Velvet Beans (Mucuna pruriens) and Velvet Beans (Mucuna pruriens) in Coffee Preparations

Biomedika ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 117-122
Author(s):  
Indah Tri Susilowati ◽  
Purwati Purwati
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Mucuna Pruriens ◽  
Methanol Extracts ◽  
Activity Test ◽  
Antioxidant Content ◽  
Ic50 Values

Antioxidant activity tests of methanol extracts of velvet beans (Mucuna pruriens) and velvet beans (Mucuna pruriens) in coffee preparations have been previously carried out by several researchers. This present study aims to examine the phytochemical and antioxidant activities of methanol extracts of Mucuna pruriens and Mucuna pruriens in coffee preparations. The methanol extract was tested for the phytochemical and antioxidant activities by applying DPPH (1,1-Dhiphenyl-2-picrylhydrazil) method. The results of the phytochemical test showed that the methanol extracts of the Mucuna pruriens and Mucuna pruriens in coffee preparations contained secondary metabolites of flavonoids, alkaloids, saponins, terpenoids, and tannins. The outcomes of the antioxidant activity test revealed that the methanol extracts of the Mucuna pruriens and Mucuna pruriens in coffee preparations had antioxidant activities, as indicated by the IC50 values of 42.09 ppm and 37.23 ppm. It was also revealed that the antioxidant content of Mucuna pruriens in coffee preparations was stronger than the content in the velvet beans alone.

scholarly journals ARGINASE INHIBITORY ACTIVITY OF STEM BARK EXTRACTS OF CAESALPINIA TORTUOSA ROXB

2018 ◽  
Vol 10 (1) ◽  
pp. 130
Author(s):  
Amirah Najid ◽  
Berna Elya ◽  
Arikadia Noviani
Keyword(s):  
Ethyl Acetate ◽  
Inhibitory Activity ◽  
Phenolic Content ◽  
Colorimetric Method ◽  
Stem Bark ◽  
Phytochemical Analysis ◽  
Standard Drug ◽  
Methanol Extracts ◽  
Methanolic Extracts ◽  
Ic50 Values

Objective: This study aimed to evaluate the arginase inhibitory activity of Caesalpinia tortuosa Roxb. stem bark extracts.Methods: C. tortuosa Roxb. stem bark extracts were obtained through reflux extraction using n-hexane, ethyl acetate, and methanol and theirinhibitory activity against arginase was measured using a microplate reader at 430 nm. Active extracts were subjected to phytochemical analysisand based on the qualitative phytochemical analysis, quantitative data regarding flavonoid and phenolic contents were obtained. The total flavonoidcontent of active extracts was determined using AlCl3 colorimetric method, and the phenolic content was determined using Folin–Ciocalteu method.Results: Ethyl acetate and methanol extracts of C. tortuosa Roxb. inhibited activity of arginase with IC50 values of 33.81 and 11.58 μg/mL,respectively, nor-NOHA acetate as standard drug inhibited arginase with IC50 values of I3.77 μg/mL. Both active extracts contained saponins,tannins, and flavonoids. Ethyl acetate and methanol extracts showed a total flavonoid content of 7.41 mgQE/g and 5.05 mgQE/g and totalphenolic content of 27.55 mgGE/g and 17.16 mgGE/g, respectively. Methanol extracts had a higher inhibitory activity than ethyl acetateextracts despite having flavonoid and phenolic content, thereby suggesting no correlation between arginase inhibitory activity and flavonoidor phenolic content.Conclusion: Ethyl acetate and methanolic extracts of C. tortuosa Roxb. stem barks containing flavonoids, tannins, and saponins displayed arginaseinhibitory activity, and no correlation was observed between arginase inhibitory activity and flavonoid and phenolic content.

Sign in / Sign up

Export Citation Format

Share Document