Estimation of Vitamin E Concentration in Swine Serum by Hemolysis Test with Hydrogen Peroxide

The question of the essentiality of vitamin E in the diet has been the object of study for many years. Recently a method has become available for estimation of tocopherol (vitamin E) in the blood through its prevention of hemolysis of erythrocytes by hydrogen peroxide. The method is applicable to 0.2 ml. of blood. The hemolysis test was applied to the blood of 282 normal full-term newborn infants and 67 premature infants. Additional determinations were performed in young well babies who had been born at term and others born prematurely ranging in age from a few days to approximately 3 months. The test was also applied to blood obtained from a variety of diseases including examples of steatorrhea. A large proportion of the normal newborn and premature infants showed more than 50 per cent hemolysis as compared with normal adults in which hemolysis is uniformly less than 10 per cent. Administration of tocopherol in those tested led to prompt reversal of the test in newborns and prematures to values comparable to those found in adults. Infants fed cows' milk formulae showed significantly less hemolysis at an average age of 7 weeks and infants who were breast fed showed still less hemolysis at the same average age. Infants and children with steatorrhea showed increased hemolysis which could be reversed by administration of tocopherol. Significant hemolysis was not found in the infants and children with a variety of other diseases. The significance of these findings is discussed and a cautious attitude is expressed towards taking these results to indicate the desirability of supplementing the diets of artifically fed infants with tocopherol.

Download Full-text

The red cell hydrogen peroxide hemolysis test and vitamin E absorption in the differential diagnosis of jaundice in infancy

The Journal of Pediatrics ◽

10.1016/s0022-3476(72)80235-x ◽

1972 ◽

Vol 81 (6) ◽

pp. 1082-1087 ◽

Cited By ~ 16

Author(s):

David K. Melhorn ◽

Samuel Gross ◽

Robert J. Izant

Keyword(s):

Hydrogen Peroxide ◽

Differential Diagnosis ◽

Vitamin E ◽

Red Cell ◽

Hemolysis Test

Download Full-text

PHYSIOLOGIC HYPERBILIRUBINEMIA AND HEMOLYSIS OF ERYTHROCYTES BY HYDROGEN PEROXIDE SOLUTION IN THE NEWBORN: INVESTIGATION OF A POSSIBLE RELATIONSHIP

PEDIATRICS ◽

10.1542/peds.20.1.92 ◽

1957 ◽

Vol 20 (1) ◽

pp. 92-97

Author(s):

Jo-Anne E. Richards ◽

Richard B. Goldbloom ◽

Ronald L. Denton

Keyword(s):

Hydrogen Peroxide ◽

Statistical Analysis ◽

Vitamin E ◽

Clinical Significance ◽

Newborn Infant ◽

Newborn Infants ◽

Control Group ◽

Term Infants ◽

Mean Values ◽

Relative Deficiency

Forty-three full-term infants have been studied with respect to hemolysis of erythrocytes in solutions of hydrogen peroxide and concentrations of bilirubin in the serum. Mean values for concentration of bilirubin in the serum and percentage of hemolysis followed similar patterns in the first few days of life. However, statistical analysis of the data in individual cases showed no significant correlation between the degree of hemolysis in solutions of hydrogen peroxide and the concentrations of bilirubin in the serum. Administration of vitamin E prevented an increase in hemolysis of erythrocytes in solutions of hydrogen peroxide but failed to produce any significant change in concentrations of bilirubin as compared with the control group. The evidence suggests that the relative deficiency of vitamin E which exists in most newborn infants does not play a part in the causation or maintenance of physiologic hyperbilirubinemia. The clinical significance of increased hemolysis of the erythrocytes of the newborn infant in solutions of hydrogen peroxide remains a mystery. Possible approaches to the clarification of this problem are suggested.

Download Full-text

In vitro Effects of Vitamin E on Hydrogen Peroxide

The Tohoku Journal of Experimental Medicine ◽

10.1620/tjem.111.99 ◽

1973 ◽

Vol 111 (1) ◽

pp. 99-100

Author(s):

OTOTAKA HIGASHI ◽

YOKO KIKUCHI

Keyword(s):

Hydrogen Peroxide ◽

Vitamin E ◽

In Vitro Effects

Download Full-text

Cytoprotective role of vitamin E in porcine adipose-tissue-derived mesenchymal stem cells against hydrogen-peroxide-induced oxidative stress

Cell and Tissue Research ◽

10.1007/s00441-018-2857-3 ◽

2018 ◽

Vol 374 (1) ◽

pp. 111-120 ◽

Cited By ~ 7

Author(s):

Fazal Ur Rehman Bhatti ◽

Song Ja Kim ◽

Ae-Kyung Yi ◽

Karen A. Hasty ◽

Hongsik Cho

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Adipose Tissue ◽

Vitamin E

Download Full-text

Cover Picture: Selective Iron-Catalyzed Oxidation of Phenols and Arenes with Hydrogen Peroxide: Synthesis of Vitamin E Intermediates and Vitamin K3 (Chem. Eur. J. 34/2010)

Chemistry - A European Journal ◽

10.1002/chem.201090167 ◽

2010 ◽

Vol 16 (34) ◽

pp. 10261-10261

Author(s):

Konstanze Möller ◽

Gerrit Wienhöfer ◽

Kristin Schröder ◽

Benoît Join ◽

Kathrin Junge ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Vitamin E ◽

Vitamin K3 ◽

Oxidation Of Phenols ◽

Hydrogen Peroxide Synthesis ◽

Catalyzed Oxidation

Download Full-text

STUDY OF THE KINETICS OF ENZYMATIC OXIDATION OF 2,3,6-TRIMETHYLPHENOL IN THE PRESENCE OF IMMOBILIZED PEROXIDASE

Вестник Тверского государственного университета. Серия: Химия ◽

10.26456/vtchem2020.4.1 ◽

2020 ◽

pp. 7-15

Author(s):

Ольга Валентиновна Гребенникова ◽

Александрина Михайловна Сульман ◽

Владимир Петрович Молчанов ◽

Елена Михайловна Михайлова ◽

Ирина Павловна Шкилева ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Vitamin E ◽

Magnetic Nanoparticles ◽

Biologically Active ◽

Enzymatic Oxidation ◽

Stable Operation ◽

Substituted Phenols ◽

Active Compounds ◽

Enzyme Systems ◽

Kinetics Of

В работе изучался процесс окисления 2,3,6-триметилфенола в присутствии иммобилизованной пероксидазы корня хрена. В результате такого окисления был получен полупродукт витамина Е (2,3,5-триметилгидрохинон). Иммобилизация фермента проводилась на магнитные наночастицы, на SiO и AlO. Все исследованные биокатализаторы показали стабильную работу в процессе окисления 2,3,6-триметилфенола с помощью пероксида водорода. Таким образом, синтезированные биокаталитические ферментные системы могут успешно применяться в качестве альтернативных катализаторов окисления замещенных фенолов с целью получения биологически активных соединений класса витаминов. We studied the oxidation of 2,3,6-trimethylphenol in the presence of immobilized horseradish root peroxidase. As a result of this oxidation, a semi-product of vitamin E (2,3,5-trimethylhydroquinone) was obtained. The enzyme was immobilized on magnetic nanoparticles, on SiO and AlO. All the studied biocatalysts showed stable operation during the oxidation of 2,3,6-trimethylphenol using hydrogen peroxide. Thus, the synthesized biocatalytic enzyme systems can be successfully used as alternative catalysts for the oxidation of substituted phenols in order to obtain biologically active compounds of the vitamin class.

Download Full-text