scholarly journals High Fiber Diets Enhance Gene Expression and Il-10 Level on Hyperlipidemic Rats Model

2020 ◽  
pp. 471-478
Author(s):  
Rifkind Malik ◽  
Ahmad Hamim Sadewa ◽  
Sunarti Sunarti
Keyword(s):  
Gene Expression ◽  
Intestinal Bacteria ◽  
Short Chain Fatty Acids ◽  
Trans Fat ◽  
High Fat ◽  
High Fiber ◽  
Fiber Diet ◽  
Before And After ◽  
Male Wistar Rats ◽  
High Fiber Diet

Hyperlipidemia induces inflammation by increasing proinflammatory cytokines and reducing anti-inflammatory cytokines. Short chain fatty acids from fiber fermented by intestinal bacteria can reduce inflammation. The aim of this study is to evaluate the benefits of high fiber diet on IL-10 gene expression and IL-10 levels in white adipose tissue in rats with high fat and fructose diet. Twenty-five male Wistar rats, were divided into 5 groups: 1) normal (N); 2) Hyperlipidemia (HL); 3) Hyperlipidemia with fiber 1.04 g/rat/day (HL1); 4) Hyperlipidemia with fiber 2.07 g/rat/day (HL2), and 5) Hyperlipidemia with fiber 3.11 g/rat/day (HL3). The rats were considered hyperlipidemia, if their plasma triglyceride levels were > 70.79 mg/dL. High fat and fructose diet contain high fructose and trans-fat, while intervention diet for T1, T2, and T3 contain a total fiber of 6.88%, 13.77% and 20.65%, respectively. Serum IL-10 analysis using ELISA method was done before and after intervention, and IL-10 gene expression was analyzed using qPCR method after intervention. High trans-fat and fructose diet decrease IL-10 levels, while high-fiber diet can significantly increase the gene expression and levels of IL-10 in hyperlipidemia rats (p<0.05). High-fiber diet can improve inflammation through increase the gene expression and levels of IL-10.

P063 A COMPREHENSIVE, MULTIOMIC DIET INTERVENTION STUDY COMPARING A LOW FAT, HIGH FIBER DIET TO AN IDEALIZED STANDARD AMERICAN DIET IN ULCERATIVE COLITIS PATIENTS

2020 ◽  
Vol 26 (Supplement_1) ◽  
pp. S8-S9
Author(s):  
Julia Fritsch ◽  
Alejandra Quintero ◽  
Judith Pignac-Kobinger ◽  
Luis Garces ◽  
Ana Santander ◽  
...  
Keyword(s):  
Quality Of Life ◽  
High Fat Diet ◽  
High Fat ◽  
Dietary Interventions ◽  
Low Fat ◽  
High Fiber ◽  
Fiber Diet ◽  
Omega 6 ◽  
High Fiber Diet

Abstract Background and Aims There is a lack of evidence-based dietary interventions in ulcerative colitis (UC) management. A diet high in fat and animal meat has been linked to an increased risk of UC. The aim of our study was to use a multilayered, multi-omic approach to comprehensively characterize the effect of a low fat, high fiber diet or a high fat diet in UC patients. Methods We enrolled patients with UC who were in remission or had mild disease with a flare within the last 18 months. We used a cross-over design in which patients received two dietary interventions: a low fat diet (LFD), containing 10% total calories from fat with an omega 6 to 3 ratio of below 3:1, and an idealized standard American diet (SAD), containing 35–40% total calories from fat with an omega 6 to 3 ratio of 20–30:1. Each diet was four weeks long with a two-week wash-out in between. The diet was catered and delivered to patients’ homes. Clinical symptoms, quality of life, and biochemical data were collected. Stool was collected for microbiome and metabolomic analyses. The primary endpoint was to determine adherence to a specified diet using catered meals; the secondary endpoint was to determine the clinical and subclinical effects of a low fat, high fiber diet or high fat diet in UC. Results Baseline diets varied widely but were generally lower in fiber as well as fruits and vegetables and higher in saturated fat than either of the study diets. There was a high rate of adherence to catered meals (SAD=86.68%, LFD=84.8%) with a 96.8% and 94.33% adherence to fat for SAD and LFD respectively. Patients that started in remission remained in remission (partial Mayo and sIBDQ). Following a LFD, patients saw a 20% improvement in their quality of life as measured by sIBDQ compared to their baseline. The effect of diet intervention on microbial diversity was reflected in the beta diversity with a significant increase in Faecalibacterium prausnitzii after LFD. CRP, sIBDQ, IL-6, and IL1β had a significant effect on overall gut microbiota composition as measured by Bray Curtis beta diversity (PERMANOVA)(P&lt;0.007, P&lt;0.001, P&lt;0.021, P&lt;0.048 respectively). The top taxa that contributes the most to this microbial variation from these clinical parameters was Faecalibacterium prausnitzii. Patients following a SAD had an increase in lauric acid, myristic acid, and N-oleoyl-L-phenylalanine with an increase in omega-6 metabolism pathways. Patients following a LFD had higher glycine, alanine, and phenyllactic acid with omega 3 metabolism pathways increased after LFD. Conclusions A low fat, high fiber diet is well tolerated and did not increase biochemical markers of inflammation. Catered meals and collection of microbiome, metabolome and biochemical data may allow early stratification of diet responders.

0952 Intestinal microbiota, microbial metabolites and carcass traits are changed in a pig model fed a high-fat/low-fiber or a low-fat/high-fiber diet

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 458-458
Author(s):  
S. N. Heinritz ◽  
E. Weiss ◽  
M. Eklund ◽  
T. Aumiller ◽  
S. Messner ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Carcass Traits ◽  
Pig Model ◽  
Microbial Metabolites ◽  
High Fat ◽  
Low Fat ◽  
High Fiber ◽  
Fiber Diet ◽  
High Fiber Diet

scholarly journals Do colonic short-chain fatty acids contribute to the long-term adaptation of blood lipids in subjects with type 2 diabetes consuming a high-fiber diet?

2002 ◽  
Vol 75 (6) ◽  
pp. 1023-1030 ◽  
Author(s):  
Thomas MS Wolever ◽  
Katrin B Schrade ◽  
Janet A Vogt ◽  
Elizabeth B Tsihlias ◽  
Michael I McBurney
Keyword(s):  
Type 2 Diabetes ◽  
Fatty Acids ◽  
Blood Lipids ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
High Fiber ◽  
Fiber Diet ◽  
High Fiber Diet

scholarly journals Intestinal Microbiota and Microbial Metabolites Are Changed in a Pig Model Fed a High-Fat/Low-Fiber or a Low-Fat/High-Fiber Diet

PLoS ONE ◽  
2016 ◽  
Vol 11 (4) ◽  
pp. e0154329 ◽  
Author(s):  
Sonja N. Heinritz ◽  
Eva Weiss ◽  
Meike Eklund ◽  
Tobias Aumiller ◽  
Sandrine Louis ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Pig Model ◽  
Microbial Metabolites ◽  
High Fat ◽  
Low Fat ◽  
High Fiber ◽  
Fiber Diet ◽  
High Fiber Diet

scholarly journals Molecular alterations induced by a high-fat high-fiber diet in porcine adipose tissues: variations according to the anatomical fat location

BMC Genomics ◽  
2016 ◽  
Vol 17 (1) ◽  
Author(s):  
Florence Gondret ◽  
Annie Vincent ◽  
Magalie Houée-Bigot ◽  
Anne Siegel ◽  
Sandrine Lagarrigue ◽  
...  
Keyword(s):  
High Fat ◽  
Adipose Tissues ◽  
High Fiber ◽  
Molecular Alterations ◽  
Fiber Diet ◽  
High Fiber Diet

scholarly journals Microbial enterotypes in personalized nutrition and obesity management

2018 ◽  
Vol 108 (4) ◽  
pp. 645-651 ◽  
Author(s):  
Lars Christensen ◽  
Henrik M Roager ◽  
Arne Astrup ◽  
Mads F Hjorth
Keyword(s):  
Weight Loss ◽  
Gut Microbiota ◽  
Short Chain Fatty Acids ◽  
Dietary Advice ◽  
Obesity Management ◽  
Personalized Nutrition ◽  
High Fiber ◽  
Fiber Diet ◽  
High Fiber Diet ◽  
Weight Loss Strategy

Abstract Human gut microbiota has been suggested to play an important role in nutrition and obesity. However, formulating meaningful and clinically relevant dietary advice based on knowledge about gut microbiota remains a key challenge. A number of recent studies have found evidence that stratification of individuals according to 2 microbial enterotypes (dominance of either Prevotella or Bacteroides) may be useful in predicting responses to diets and drugs. Here, we review enterotypes in a nutritional context and discuss how enterotype stratification may be used in personalized nutrition in obesity management. Enterotypes are characterized by distinct digestive functions with preference for specific dietary substrate, resulting in short-chain fatty acids that may influence energy balance in the host. Consequently, the enterotype potentially affects the individual's ability to lose weight when following a specific diet. In short, a high-fiber diet seems to optimize weight loss among Prevotella-enterotype subjects but not among Bacteroides-enterotype subjects. In contrast, increasing bifidobacteria in the gut among Bacteroides-enterotype subjects improves metabolic parameters, suggesting that this approach can be used as an alternative weight loss strategy. Thus, enterotypes, as a pretreatment gut microbiota biomarker, have the potential to become an important tool in personalized nutrition and obesity management, although further interventions assessing their applicability are warranted.

scholarly journals High-fiber diets attenuate emphysema development via modulation of gut microbiota and metabolism

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yoon Ok Jang ◽  
Ock-Hwa Kim ◽  
Su Jung Kim ◽  
Se Hee Lee ◽  
Sunmi Yun ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Dietary Fiber ◽  
Therapeutic Potential ◽  
Treatment Plan ◽  
Short Chain Fatty Acids ◽  
High Fiber ◽  
Microbial Dysbiosis ◽  
Dietary Nutrients ◽  
Fiber Diet ◽  
High Fiber Diet

AbstractDietary fiber functions as a prebiotic to determine the gut microbe composition. The gut microbiota influences the metabolic functions and immune responses in human health. The gut microbiota and metabolites produced by various dietary components not only modulate immunity but also impact various organs. Although recent findings have suggested that microbial dysbiosis is associated with several respiratory diseases, including asthma, cystic fibrosis, and allergy, the role of microbiota and metabolites produced by dietary nutrients with respect to pulmonary disease remains unclear. Therefore, we explored whether the gut microbiota and metabolites produced by dietary fiber components could influence a cigarette smoking (CS)-exposed emphysema model. In this study, it was demonstrated that a high-fiber diet including non-fermentable cellulose and fermentable pectin attenuated the pathological changes associated with emphysema progression and the inflammatory response in CS-exposed emphysema mice. Moreover, we observed that different types of dietary fiber could modulate the diversity of gut microbiota and differentially impacted anabolism including the generation of short-chain fatty acids, bile acids, and sphingolipids. Overall, the results of this study indicate that high-fiber diets play a beneficial role in the gut microbiota-metabolite modulation and substantially affect CS-exposed emphysema mice. Furthermore, this study suggests the therapeutic potential of gut microbiota and metabolites from a high-fiber diet in emphysema via local and systemic inflammation inhibition, which may be useful in the development of a new COPD treatment plan.

scholarly journals Impact of a High-Fat or High-Fiber Diet on Intestinal Microbiota and Metabolic Markers in a Pig Model

Nutrients ◽  
2016 ◽  
Vol 8 (5) ◽  
pp. 317 ◽  
Author(s):  
Sonja Heinritz ◽  
Eva Weiss ◽  
Meike Eklund ◽  
Tobias Aumiller ◽  
Charlotte Heyer ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Pig Model ◽  
High Fat ◽  
Metabolic Markers ◽  
High Fiber ◽  
Fiber Diet ◽  
High Fiber Diet
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