scholarly journals IMPACT OF PHYSICAL MUTAGENS ON THE MALE GAMETOPHYTE OF THE TEA PLANT FOR ITS FURTHER USE IN HYBRIDIZATION

2019 ◽  
Vol 7 ◽  
pp. 880-883
Author(s):  
Davit Baratashvili ◽  
Nino Lomtatidze ◽  
Guguli Dumbadze ◽  
Neli Khalvashi ◽  
Nino Kedelidze
Keyword(s):  
Pollen Tube ◽  
Male Gametophyte ◽  
Tea Plant ◽  
Control Group ◽  
Primary Nucleus ◽  
Slow Development ◽  
Migration Intensity ◽  
Pollen Sacs

The migration of the radioisotopes - P32 and S35 from the soil to the tea plant (flowers) was studied. It is shown that their accumulation intensity in pollen sacs increases in proportion to the dose and reaches its maximum in the P32 variant after using a 8.0 MBq / ml dose. The migration intensity was the lowest in the 2.0 MBq / ml variant (0.14-0.223 MBq / 100 mg). It is estimated that P32 and S35 have significantly affected the vitality of tea plant pollens through the anomalies of their development. After using 2.0 and 4.0 MBq / ml doses, the vitality of the pollen is reduced in proportion to the dose and the development anomalies increase. Particulalry, the viability of pollen decrease compared to the control group, but the frequency and range of development anomalies increases. These include the slow development of the plant or the acceleration of the development of the primary nucleus, polyspermia, undeveloped pollen tube, etc. The use of processed P32 and S35 tea pollens in hybridization significantly increases the frequency of new formations and results in variability in the domination of signs.  Among the new formations include the induction of haploids and triploids.

scholarly journals Protein Analysis of Pollen Tubes after the Treatments of Membrane Trafficking Inhibitors Gains Insights on Molecular Mechanism Underlying Pollen Tube Polar Growth

2021 ◽  
Vol 40 (2) ◽  
pp. 205-222
Author(s):  
Monica Scali ◽  
Alessandra Moscatelli ◽  
Luca Bini ◽  
Elisabetta Onelli ◽  
Rita Vignani ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Actin Cytoskeleton ◽  
Membrane Trafficking ◽  
Tip Growth ◽  
Male Gametophyte ◽  
Pollen Tubes ◽  
Structural Features ◽  
Two Dimensional Gel Electrophoresis ◽  
Depth Analysis

AbstractPollen tube elongation is characterized by a highly-polarized tip growth process dependent on an efficient vesicular transport system and largely mobilized by actin cytoskeleton. Pollen tubes are an ideal model system to study exocytosis, endocytosis, membrane recycling, and signaling network coordinating cellular processes, structural organization and vesicular trafficking activities required for tip growth. Proteomic analysis was applied to identifyNicotiana tabacumDifferentially Abundant Proteins (DAPs) after in vitro pollen tube treatment with membrane trafficking inhibitors Brefeldin A, Ikarugamycin and Wortmannin. Among roughly 360 proteins separated in two-dimensional gel electrophoresis, a total of 40 spots visibly changing between treated and control samples were identified by MALDI-TOF MS and LC–ESI–MS/MS analysis. The identified proteins were classified according to biological processes, and most proteins were related to pollen tube energy metabolism, including ammino acid synthesis and lipid metabolism, structural features of pollen tube growth as well modification and actin cytoskeleton organization, stress response, and protein degradation. In-depth analysis of proteins corresponding to energy-related pathways revealed the male gametophyte to be a reliable model of energy reservoir and dynamics.

The development of the male gametophyte and spermatogenesis in Taxus baccata L

1986 ◽  
Vol 228 (1250) ◽  
pp. 85-96 ◽  
Keyword(s):  
Pollen Tube ◽  
De Novo ◽  
Male Gametophyte ◽  
Generative Cell ◽  
Equal Size ◽  
Taxus Baccata ◽  
Transverse Wall ◽  
Proximal Pole ◽  
Spermatogenous Cell ◽  
Tube Cell

The development of the male gametophyte of Taxus baccata has been studied over a period of 20 weeks, from germination of the microspore in February to spermatogenesis in July. A few days after germination the microspore nucleus divides and a transverse wall forms at the equator cutting off the small generative cell and a large tube cell. The latter immediately begins to expand to form the pollen tube. The first division thus establishes the polarity of the gametophyte and the generative cell is regarded as proximal. The transverse wall is ephemeral, and within six weeks it has disappeared. The nucleus of the generative cell divides while still at the proximal pole. The two daughter nuclei are unequal in size, but they remain associated and together move distally. The larger nucleus eventually becomes the nucleus of the spermatogenous cell, and the smaller the sterile nucleus. The spermatogenous cell acquires a distinctive cytoplasm and becomes surrounded by a wall which arises de novo . The nucleus of the spermatogenous cell enlarges, but always remains towards one side of the cell so that at mitosis the spindle is contained within one hemisphere. After division the wall of the spermatogenous cell is ruptured and the two sperms are released as naked nuclei of equal size. The cytoplasm of the spermatogenous cell degenerates as it enters the tube, but remains recognizable until fertilization.

Insemination of the archegonium and fertilization in Taxus baccata L

1988 ◽  
Vol 89 (4) ◽  
pp. 551-560
Author(s):  
ROGER I. PENNELL ◽  
PETER R. BELL
Keyword(s):  
Pollen Tube ◽  
Male Gametophyte ◽  
Sperm Nucleus ◽  
Taxus Baccata ◽  
Close Apposition ◽  
Cell Cytoplasm ◽  
Spermatogenous Cell ◽  
Tube Lumen ◽  
Sperm Nuclei ◽  
Progressive Enlargement

A study of fertilization in Taxus baccata in the electron microscope has revealed novel features. Insemination of the archegonium is facilitated by local perforation of the wall of the young pollen tube. Digestion of the wall begins before the pollen tube pierces the megaspore membrane but is not completed until its tip makes contact with the neck cells of the archegonium. As soon as a pore is formed a single sperm nucleus and some cytoplasm of the male gametophyte enter the archegonium. Which of the paired sperm nuclei move from the pollen tube into the archegonium appears to be a matter of chance. Close apposition of sperm nucleus and egg nucleus is followed by the formation of numerous points of contact between the two. The membranes fuse at these points and pores are rapidly formed. The progressive enlargement of these pores ultimately eliminates any partitions and yields the zygotic nucleus. There is a possibility that, as in some other gymnosperms, the plastids and mitochondria of the zygote come in part from the male gametophyte, but whether from the remains of the spermatogenous cell cytoplasm or from the. pollen tube lumen is not clear.

Unusual source of food: impact of dead siblings on encapsulated embryo development of Crepipatella fecunda (Gastropoda : Calyptraeidae)

2007 ◽  
Vol 58 (12) ◽  
pp. 1152 ◽  
Author(s):  
V. M. Cubillos ◽  
O. R. Chaparro ◽  
Y. A. Montiel ◽  
D. Véliz
Keyword(s):  
Larval Development ◽  
Embryo Development ◽  
Control Group ◽  
Intermediate Period ◽  
Mechanical Disruption ◽  
Slow Development ◽  
And Control ◽  
Experimentally Induced

Embryonic cannibalism has been identified in directly developing calyptraeid species through observation of the ingestion of encapsulated sibling embryos. The object of the present study was to determine the effects of experimentally induced cannibalism on larval development in encapsulated larvae of Crepipatella fecunda (a species having mixed development). The effects studied included the time of intracapsular development, protoconch size and velar characteristics of the larvae. Mortality was induced during intracapsular development through mechanical disruption (‘treatment’) of embryos. A treatment and control group of embryos from the same female were compared. Encapsulated veligers actively fed on their sacrificed congeners. Larvae hatched in less than 10 days from treated capsules and had mean shell lengths and velum areas significantly lower than those from the control, but no significant differences in cilia length. In treated capsules where the embryos underwent a slow development (>20 days), the larvae produced shells, vela and cilia larger than those of the controls. In an intermediate period of intracapsular development, the differences recorded among larval characters were not statistically significant. The results showed that: (1) the encapsulated veligers were capable of feeding on exogenous food before hatching; (2) the consumption of non-living congeners decreased the time of intracapsular development; and (3) the morphometry of the larvae hatching from treated capsules varied depending on the period of intracapsular development and seems to be adaptively stabilised towards homogeneous larval morphometry.

La morphogenèse florale chez le pétunia. III. Déviations dans le programme organogène chez un mutant femelle stérile

1991 ◽  
Vol 69 (4) ◽  
pp. 866-872 ◽  
Author(s):  
Marie-France Turlier ◽  
Josiane Alabouvette ◽  
Diane Doulain-Douvier
Keyword(s):  
Key Words ◽  
Petunia Hybrida ◽  
Male Gametophyte ◽  
Female Sterility ◽  
Pollen Sacs

A monogenic mutant of Petunia hybrida R-n57 exhibits flowers with abnormal gynoecium: various malformations, such as the opening of the style and the nonprotection of ovules, or the masculinization with development of pollen sacs, anthers, and male gametophyte growing with or within ovules. The same type of abnormalities happens over and over along one inflorescential axis so that an ontogenic analysis can be conducted. Some assumptions are supported by the histocytological study: a few punctual modifications of the normal organogenesis program, recently established, and one new stage ending at the masculine or abnormal gynoecium, with homeotic members, are involved. Relations between the deflected development and female sterility are argued. Key words: flower, teratology, morphogenesis, mutant, Petunia.

scholarly journals Attractive and repulsive interactions between female and male gametophytes in Arabidopsis pollen tube guidance

Development ◽  
2000 ◽  
Vol 127 (20) ◽  
pp. 4511-4518 ◽  
Author(s):  
K.K. Shimizu ◽  
K. Okada
Keyword(s):  
Pollen Tube ◽  
Female Gametophyte ◽  
Inclusive Fitness ◽  
Male Gametophyte ◽  
Pollen Tubes ◽  
Interspecific Crosses ◽  
Pollen Tube Guidance ◽  
Normal Number ◽  
Mutant Female ◽  
Diploid Cells

Sexual reproduction in plants, unlike that of animals, requires the action of multicellular haploid gametophytes. The male gametophyte (pollen tube) is guided to a female gametophyte through diploid sporophytic cells in the pistil. While interactions between the pollen tube and diploid cells have been described, little is known about the intercellular recognition systems between the pollen tube and the female gametophyte. In particular, the mechanisms that enable only one pollen tube to interact with each female gametophyte, thereby preventing polysperm, are not understood. We isolated female gametophyte mutants named magatama (maa) from Arabidopsis thaliana by screening for siliques containing half the normal number of mature seeds. In maa1 and maa3 mutants, in which the development of the female gametophyte was delayed, pollen tube guidance was affected. Pollen tubes were directed to mutant female gametophytes, but they lost their way just before entering the micropyle and elongated in random directions. Moreover, the mutant female gametophytes attracted two pollen tubes at a high frequency. To explain the interaction between gametophytes, we propose a monogamy model in which a female gametophyte emits two attractants and prevents polyspermy. This prevention process by the female gametophyte could increase a plant's inclusive fitness by facilitating the fertilization of sibling female gametophytes. In addition, repulsion between pollen tubes might help prevent polyspermy. The reproductive isolations observed in interspecific crosses in Brassicaceae are also consistent with the monogamy model.

scholarly journals The Effect of The Touché Therapy On The Anthropometry for Infants In Working Area Service of The PublicHealthCenterPesantren I Kediri.

1970 ◽  
Vol 5 (1) ◽  
pp. 122
Author(s):  
Heny Kristanto
Keyword(s):  
Infant Development ◽  
Sampling Technique ◽  
Total Sample ◽  
Brain Cell ◽  
Control Group ◽  
The Public ◽  
Slow Development ◽  
Dependent Sample ◽  
And Control ◽  
Hormonal Development

The hindrance of infant development is caused by unbearable environmental factor. From year to year, it has been recorded that the infant natal figure is still high, including the natal figure in Kediri – City. Since the high infant natal figure and the infant age as the potential period for development, therefore, it should be optimally cared the development for infant, one of them is done by touché therapy. The objective of the research is to prove that there is a difference between infant cared with anthropometry therapy using touché therapy in working area service of the Public Health Center Pesantren I Kediri. The research plan uses the Randomized Control Trial, the collecting sampling technique uses Total Sampling, and the total sample is 20 infant. Variable research is touché therapy (independent variable) and infant anthropometry (variable dependent). From the result of processing data of anthropometry by using ANOVA test and T test on dependent sample, it can be obtained the significant improvement on the height of the infant (for the treatment group on the 4th week observation, the control group on the 5th week observation and the control group on the 6th  week observation). No significant improvement for the head circle of the infant for the treatment control group. The significant improvement on the height is caused by the secretion of Neuro-chemical beta-endorphin the result of the touché therapy treatment, and later it can cause the discretion of hormonal development. The significant improvement on the weight is caused by the stimulus of the nervous vagus that can increase the level of gastric and insulin absorption enzyme. No significant improvement on the head circle of the infantfor the treatment and control groups is caused by the very slow development of the brain cell.; key words: Touché therapy,  Anthropometry

scholarly journals Fused Vein Trait in Cucurbita pepo L. Associated with Subvitality of the Male Gametophyte

1996 ◽  
Vol 121 (1) ◽  
pp. 18-22 ◽  
Author(s):  
R. Bruce Carle ◽  
J. Brent Loy
Keyword(s):  
Pollen Tube ◽  
Seed Yield ◽  
Microscopic Examination ◽  
Cucurbita Pepo ◽  
Male Gametophyte ◽  
Pollen Competition ◽  
Pollen Load ◽  
Pollen Loads ◽  
Normal Pollen

Two experiments were conducted to test and delineate gametophytic subvitality of the fused vein trait in Cucurbita pepo. Gametophytic subvitality was verified by comparing pollen tube growth for fused vein and normal pollen in situ. Microscopic examination of partitioned, co-pollinated distillate flowers revealed inferior fused vein gametophyte performance. Normal pollen tubes grew faster and were significantly more abundant in the lower portion of the style. The consequences of gametophytic subvitality on seed yield and inheritance were shown by manipulating the severity of pollen competition. Fused vein, normal and F1 lines were pollinated with fused vein, normal, F and a 50:50 pollen mix at three different pollen loads. Fused vein pollen generated significantly fewer seed per fruit in all female genotypes. As a constituent in F, or mixed pollen, it produced significant seed yield reductions at the low pollen load. In F1 and testcross populations, a reduction in pollen load and therefore pollen competition significantly increased the number of fused vein individuals in segregating populations.

scholarly journals Anatomical Characteristics of Petalized Anther Abortion in Male Sterile Camellia oleifera Plants

2021 ◽  
pp. 1-13
Author(s):  
Yang Hu ◽  
Chao Gao ◽  
Quanen Deng ◽  
Jie Qiu ◽  
Hongli Wei ◽  
...  
Keyword(s):  
Germination Rate ◽  
Pollen Grains ◽  
Relative Activity ◽  
Control Group ◽  
Camellia Oleifera ◽  
Male Sterile ◽  
Flower Bud ◽  
Triphenyltetrazolium Chloride ◽  
Pollen Sacs

Petalized anther abortion is an important characteristic of male sterility in plants. The male sterile plants (HB-21) evincing petalized anther abortion previously discovered in a clone population of the Camellia oleifera cultivar Huashuo by our research group were selected as the experimental material in this study. Using plant microscopy and anatomic methods and given the correspondence between external morphology and internal structure, we studied the anatomic characteristics of petalized anther abortion (with a fertile plant as the control group) in various stages, from flower bud differentiation to anther maturity, in hopes of providing a theoretical basis for research on and applications of male sterile C. oleifera plants, a new method for the selection of male sterile C. oleifera cultivars, and improvements in the yield and quality of C. oleifera. In this study, the development of anthers in C. oleifera was divided into 14 stages. Petalized anther abortion in male sterile plants was mainly initiated in the second stage (the stage of sporogenous cells). Either the petalized upper anther parts did not form pollen sacs, or the entire anthers did not form pollen sacs. The lower parts of some anthers could form deformed pollen sacs and develop, and these anthers could be roughly divided into two types: fully and partially petalized anthers. Abnormal callose and the premature degradation of the tapetum occurred in the pollen sacs formed by partially petalized anthers during the development process, resulting in the absence of inclusions in the pollen grains formed. Small quantities of mature pollen grains withered inward from the germinal furrows, exhibiting obvious abortion characteristics. The relative in vitro germination rate of the pollen produced by the partially petalized anthers of sterile plants was 11.20%, and the relative activity of triphenyltetrazolium chloride was 3.24%, while the fully petalized anthers did not generate pollen grains. Either the petalized anthers in male sterile plants did not produce pollen, or the vitality of the small amounts of pollen produced by sterile plants was very low compared with that of fertile plants. Such male sterile plants could be used to select correct clones and have good prospects for application in production.

scholarly journals Influence of change in the proportion of H1 histone variants on microsporogenesis and development of male gametophyte in transgenic plants of tobacco (Nicotiana tabacum L.)

2011 ◽  
Vol 72 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Joanna Ślusarczyk ◽  
Andrzej Wierzbicki ◽  
Marcin Przewłoka ◽  
Teresa Tykarska ◽  
Andrzej Jerzmanowski ◽  
...  
Keyword(s):  
Male Gametophyte ◽  
Pollen Grains ◽  
Histone Variants ◽  
Ultrastructural Changes ◽  
Control Group ◽  
H1 Histone ◽  
Nicotiana Tabacum L ◽  
Sterile Pollen ◽  
Different Levels

As continuation of investigations in to the mechanism of the role of the H1 histone, which is a crucial protein component chromosomes of all eukaryotes, transgenic tobacco plants with different levels of the H1 histone variants were examined. Tobacco has six sequential variants of the H1 histone: two major ones (H1A and H1B), constituting ca. 90% of all H1, and four minor ones (H1C, H1D, H1E and H1F), occurring in very small quantities. The following groups of plants were examined: K - control group with a full set of histone variants; -AB -with the A and B variants removed; -ABCD - with the A, B, C and D variants removed; and -CD - with the C and D variants removed. The analysis of microsporogenesis in those plants, based on preparations squeezed in acetoorcein, revealed the asynchronous course of meiosis in -AB and -ABCD plants, occurrence of chromosomal aberration, and, consequently, the formation of sterile pollen grains (accordingly: 84,4% and 81,4%). In -CD plants, the percentage of aberration and sterile pollen grains was similar to the control material. Electron microscope observations of microsporogenesis showed ultrastructural changes. In -AB and -ABCD plants, a major portion of the pollen grains were degraded. The smallest number of degraded pollen grains, in comparison with the control, was found in the -CD group.

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