scholarly journals Evaluation of Anticariogenic Efficacy of Pomegranate (Punica Granatum) Pericarp Extract as Natural Mouth Rinse: An In Vitro and In Vivo Study

2018 ◽  
Vol 11 (4) ◽  
pp. 2025-2030
Author(s):  
Jyothsna Pinni ◽  
J.S. Sankar Avula ◽  
Sridhar Mukthineni ◽  
Sujatha Bandi ◽  
Thulasi Gokul
Keyword(s):  
Antimicrobial Efficacy ◽  
Mouth Rinse ◽  
Plant Materials ◽  
Group Iii ◽  
Group I ◽  
Mouth Rinses ◽  
Significant Difference ◽  
Mouth Rinsing

Plant materials were known as source of new antimicrobial agents. Many efforts have been made to develop alternative mouth rinses from natural products which are safe, easily available and substitute the standard pharmaceutical remedies. Thus, considering the therapeutic value of pomegranate fruit, the present study was designed to compare the antimicrobial efficacy of aqueous extract of Pomegranate pericarp and commercially available Chlorhexidine mouth wash against caries causative microorganisms both in vitro and in vivo. To evaluate the antimicrobial efficacy of pomegranate pericarp extract (PPE) against Streptococcus mutans (S.mutans) and to determine its usefulness as anti caries mouth rinse. In vitro antimicrobial efficacy was evaluated by disc inhibition zone method and broth dilution assay considering minimum inhibitory concentration of PPE. In vivo evaluation was done as a randomised controlled trial which included thirty children aged between 6-12 years. They were divided randomly into 3 groups of ten each and subjected to different mouth rinses - Group I: PPE mouth rinse, Group II: 0.2% Chlorhexidine mouth rinse and Group III: Distilled water (control). The salivary samples which were collected before and after (5 minutes) mouth rinsing were inoculated on Mutans Sanguis agar and the bacterial count was calculated.Statistically significant decrease in salivary S.mutans count was observed in group I and II compared to group III after mouth rinsing. However, there was no statistically significant difference between groups I and II. PPE mouthwash was effective in reducing the salivary S.mutans count and was comparable to chlorhexidine mouth rinse. Hence PPE mouth rinse may be considered as a potential anti caries mouth rinse.

scholarly journals Pentoxifylline Attenuates Methionine- and Choline-Deficient-Diet-Induced Steatohepatitis by Suppressing TNF-αExpression and Endoplasmic Reticulum Stress

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Min Kyung Chae ◽  
Sang Gyu Park ◽  
Sun-Ok Song ◽  
Eun Seok Kang ◽  
Bong Soo Cha ◽  
...  
Keyword(s):  
Er Stress ◽  
Deficient Diet ◽  
Group Iii ◽  
Unfolded Protein ◽  
Sd Rats ◽  
Group I ◽  
Hep3b Cells ◽  
Protein Response

Background. Pentoxifylline (PTX) anti-TNF properties are known to exert hepatoprotective effects in various liver injury models. The aim of this study was to investigate whether PTX has beneficial roles in the development of methionine- and choline-deficient-(MCD-) diet-induced NAFLD SD ratsin vivoand TNF-α-induced Hep3B cellsin vitro.Methods. SD Rats were classified according to diet (chow or MCD diet) and treatment (normal saline or PTX injection) over a period of 4 weeks: group I (chow + saline,n=4), group II (chow + PTX), group III (MCD + saline), and group IV (MCD + PTX). Hep3B cells were treated with 100 ng/ml TNF-α(24 h) in the absence or presence of PTX (1 mM).Results. PTX attenuated MCD-diet-induced serum ALT levels and hepatic steatosis. In real-time PCR and western blotting analysis, PTX decreased MCD-diet-induced TNF-alpha mRNA expression and proapoptotic unfolded protein response by ER stress (GRP78, p-eIF2, ATF4, IRE1α, CHOP, and p-JNK activation)in vivo. PTX (1 mM) reduced TNF-α-induced activation of GRP78, p-eIF2, ATF4, IRE1α, and CHOPin vitro.Conclusion. PTX has beneficial roles in the development of MCD-diet-induced steatohepatitis through partial suppression of TNF-αand ER stress.

scholarly journals Comparative Evaluation of Antibacterial Efficacy of Four Toothpastes and Mouthwashes against Streptococcus mutans and Lactobacillus: An in vivo Study

2016 ◽  
Vol 1 (2) ◽  
pp. 60-65
Author(s):  
Jonathan E Sam ◽  
Paulaian Benin ◽  
Ruth H Beaulah ◽  
Gnanaseelan LNU ◽  
Lal Krishna ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Comparative Evaluation ◽  
Antibacterial Efficacy ◽  
Time Intervals ◽  
Group Iii ◽  
Antimicrobial Substances ◽  
Group I ◽  
Intergroup Comparison ◽  
Significant Difference

ABSTRACT Background Cariogenic microorganisms are the most important cause for occurrence of dental caries. Dentifrices and mouthwashes containing antimicrobial substances are proven to be effective in the eradication of these pathogens from the oral cavity. Aim To evaluate the antimicrobial efficacy of fluoride, chlorhexidine (CHX), herbal, and xylitol containing toothpastes and mouthwashes against Streptococcus mutans (S. mutans) and Lactobacillus (LB) in subjects within the age group of 18 to 22 years at time intervals of 1, 3, and 6 months. Materials and methods One hundred subjects were randomly divided into four groups. Group I: fluoride, group II: chlorhexidine, group III: herbal, group IV: xylitol and instructed to use toothpastes and mouthwashes containing the specific agents. Salivary samples were collected to evaluate the levels of S. mutans and LB at baseline, 1, 3, and 6 months. Bacterial levels were evaluated using caries risk test (CRT) kit. Data were analyzed using analysis of variance and post hoc test. Results During intragroup comparison, S. mutans levels in group I showed statistically significant difference among the four time intervals. On intergroup comparison, S. mutans levels after 6 months for groups I, II, III, and IV were 1.12, 1.16, 1.28, and 1.4 respectively. Conclusion It can be concluded that fluoride, CHX, and xylitol showed a significant reduction in S. mutans and LB count after a time period of 6 months while herbal group did not show a significant reduction in S. mutans and LB count at any intervals. How to cite this article Sam JE, Benin P, Beaulah RH, Gnanaseelan, Krishna L, Raja J. Comparative Evaluation of Antibacterial Efficacy of Four Toothpastes and Mouthwashes against Streptococcus mutans and Lactobacillus: An in vivo Study. J Oper Dent Endod 2016;1(2):60-65.

scholarly journals An in vitro comparative Evaluation of Fracture Strength of Roots Instrumentated with Self-adjusting File and Reciproc Reciprocating File, with and without Obturation

2017 ◽  
Vol 1 (1) ◽  
pp. 20-25
Author(s):  
Pooja Kabra
Keyword(s):  
Fracture Strength ◽  
Comparative Evaluation ◽  
Group Iv ◽  
Control Group ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Group Ii

ABSTRACT Aim The purpose of this study was to evaluate the fracture strength of roots instrumented with the self-adjusting file (SAF; ReDent-Nova, Ra'anana, Israel) and the Reciproc reciprocating file and that were and were not obturated using the warm vertical lateral compaction technique. Materials and methods In total, 75 mandibular premolar teeth were sectioned at or below the cementoenamel junction to obtain roots 13 mm in length. The roots were balanced with respect to buccolingual and mesiodistal diameters and weight. They were distributed into four experimental groups and one control group (n = 15): No instrumentation (group I), instrumentation with SAF files but no obturation (group II), instrumentation with SAF files and obturated with warm vertical lateral compaction (group III), instrumentation with Reciproc File but no obturation (group IV), and instrumentation with Reciproc File and obturated with warm vertical lateral compaction (group V). AH Plus sealer (Dentsply DeTrey, Konstanz, Germany) was used along with gutta-percha points. One week later, a vertical load was applied to the specimen's canal until fracture occurred. Data were statistically analyzed using one-way analysis of variance (p = 0.05). Results The mean fracture load was 312.83 N for group I, 297.35 N for group II, 359.15 N for group III, 231.51 N for group IV, and 275.81 N for group V. Conclusion The fracture resistances exhibited a statistically significant difference between all the groups. Teeth instrumented by SAF exhibited a better fracture resistance. How to cite this article Tyagi S, Choudhary E, Kabra P, Chauhan R. An in vitro comparative Evaluation of Fracture Strength of Roots Instrumentated with Self-adjusting File and Reciproc Reciprocating File, with and without Obturation. Int J Clin Dent Res 2017;1(1):20-25.

scholarly journals Transverse Strength of Heat Cured Acrylic Resin After Immersed in 0, 2% Chlorhexidine Gluconate Mouthwash (In Vitro)

2017 ◽  
Vol 1 ◽  
Author(s):  
Annisa Mayang Rusdi ◽  
Lasminda Syafiar ◽  
Astrid Yudhit
Keyword(s):  
Testing Machine ◽  
Acrylic Resin ◽  
Chlorhexidine Gluconate ◽  
P Value ◽  
Group V ◽  
Group Iii ◽  
Transverse Strength ◽  
Group I ◽  
Significant Difference

<p class="AbstractContent"><strong>Objective:</strong> The purpose of this study was to evaluate transverse strength of heat cured acrylic resin after immersed in 0,2% chlorhexidine gluconate mouthwash for 15, 30, 45, and 60 minutes.</p><p class="AbstractContent"><strong>Methods:</strong> This was an experimental laboratory study with posttest only group design. The samples were heat cured acrylic resin plate with size 65mm x 10mm x 2,5mm. Polymerization of the heat cured acrylic was done by water bath (74 °C for 2hrs followed by 100 °C for 1hr). Totally 30 samples were prepared and divided into 5 groups (n=6) which are group I as control, and others were immersed in 0, 2% chlorhexidine gluconate, group II (15 minutes), group III (30 minutes), group IV (45 minutes) and group V (60 minutes) respectively. The transverse strength was test using Torsee’s Universal Testing machine, Japan with crosshead 1/10 mm/seconds. The data was statistically analyzed using one way ANOVA (p≤0,005).</p><p class="AbstractContent"><strong>Results:</strong> Means and Standard deviation of Transverse strength were 92.382±11.786 N/mm2, 92.186±13.349 N/mm2,92.578±12.492 N/mm2, 92.382±13.939 N/mm2, and 91.989±12.285 N/mm2 for group I, II, III, IV, and V respectively. Statistic analyzed showed no significant difference among groups with p value= 1,000 (p &lt; 0, 05).</p><p class="AbstractContent"><strong>Conclusion:</strong> The immersion in 0, 2% chlorhexidine gluconate mouthwash at different period does not changed transverse strength of heat cured acrylic resin.</p>

Effect of Bonding Agent on Retention of Different Sealants: An in Vitro Study

2021 ◽  
Vol 45 (3) ◽  
pp. 177-185
Author(s):  
Moaz H Attar ◽  
Medhat A Abdallah ◽  
Hussein A Alharthy ◽  
Omar A El Meligy
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
In Vitro Study ◽  
Bonding Agent ◽  
Micro Ct ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Sealant Retention

Objectives: To investigate the effect of Co-curing versus Staged-curing and No-bonding on retention of different resin-based sealants (RBS). Study design: For shear bond strength (SBS) and microleakage tests, 90 extracted premolars were divided equally into 3 groups (I, II, III). Each group was further subdivided equally into 3 subgroups (a, b, c). No-bonding subgroups did not receive a bonding agent, Staged-curing subgroups received a bonding agent that was cured before sealant application, while Co-curing subgroups received a bonding agent that was cured after sealant application. Seal-it was applied for group I, Helioseal-F for group II and Clinpro for group III. SBS buttons were tested using Instron machine, while microleakage specimens were examined using micro-CT. Results: Clinpro showed the highest SBS values in Staged-curing and No-bonding groups (8.72±2.39, 12.51±3.16) respectively. Staged-curing was significantly greater in SBS values than those for other groups (P&lt;0.05). There was a significant difference in microleakage values of Staged-curing among different RBS (P = 0.003), while there was no significant difference in values of No-bonding and Co-curing among different RBS (P = 0.541, P = 0.521). Conclusions: The use of a bonding agent as Staged-curing was more effective in improving sealant retention than No-bonding and Co-curing.

INTRAARTERIAL THROMBOLYSIS BY STREPTOKINASE-GLU-PLASMINOGEN

1987 ◽  
Author(s):  
E J Pilger ◽  
J Lammer ◽  
H Bertuch ◽  
H Steiner
Keyword(s):  
Artery Occlusion ◽  
Molar Ratio ◽  
In Vitro Test ◽  
Group Iii ◽  
Fibrinolytic Agent ◽  
Molar Ratios ◽  
Intraarterial Thrombolysis ◽  
Group I

In order to predict usefulness of streptokinase (SK), urokinase (UK) and streptokinase-glutamin-plasminogen (SK-Glu-Plg) for intraarterial fibrinolysis, an in vitro test was designed. Fibrin plates with and without plasminogen were incubated with SK, UK and SK-Glu-Plg (in molar ratios of 1:1, 2:1 and 1:2). On the fibrin plates containing plasminogen the highest fibrinolytic activity was observed with UK; on the fibrin plates without plasminogen, SK-Glu-Plg in a molar ratio of 1:2 was superior. We concluded, that plasmin would be synthesized by SK and Glu-Plg. In order to examine the in vivo efficacy of these different fibrinolytic agents, 120 patients suffering from peripheral artery occlusion were randomized into three treatment groups for local thrombolysis. The technique of Hess and coworkers was used for local thrombolytic therapy. In group I local fibrinolysis was performed with SK (2500 IU/5 min), in group II UK (4000 IU/5 min) was used and in group III the lytic agent consisted of SK-Glu-Plg (2500 IU/5 min). The primary recanalization rate was equal in all groups: 86%, 89% and 83% in group I, II and III respectively. However the duration required for the procedure and thus the total amount of fibrinolytic agent used was significantly different (p < 0,001) between the three groups: 2,3 ± 1,4; 2,1 ± 1,2 and 1,1 ±0,8 hours for groups I,II and III, respectively (mean ± SEM) . We conclude that SK-Glu-Plg or plasmin itself has the highest efficacy as a fibrinolytic agent for intraarterial thrombolysis. Since the intra-thrombotic concentration of Pig is unknown in an individual patient an empirically chosen dose of SK of UK may either be to high or to low for optimal thrombolysis.

scholarly journals Effectiveness of different final irrigation protocols in removing debris in flattened root canals

2009 ◽  
Vol 20 (3) ◽  
pp. 211-214 ◽  
Author(s):  
Michele Regina Nadalin ◽  
Danyel Elias da Cruz Perez ◽  
Luiz Pascoal Vansan ◽  
Cristina Paschoala ◽  
Manoel Damião Souza-Neto ◽  
...  
Keyword(s):  
Cross Sections ◽  
Control Group ◽  
Water Control ◽  
Group V ◽  
Group Iii ◽  
Root Canals ◽  
Group I ◽  
Significant Difference ◽  
Histological Processing

This study evaluated in vitro the capacity of debris removal from the apical third of flattened root canals, using different final irrigation protocols. Thirty human mandibular central incisors with a mesiodistal flattened root were prepared using rotary instrumentation by Endo-Flare 25.12 and Hero 642 30.06, 35.02, 40.02 files, irrigated with 2 mL of 1% NaOCl after each file. The specimens were randomly distributed into 5 groups according to the final irrigation of root canals: Group I: 10 mL of distilled water (control), Group II: 10 mL of 1% NaOCl for 8 min, Group III: 2 mL of 1% NaOCl for 2 min (repeated 4 times), Group IV: 10 mL of 2.5% NaOCl for 8 min, and Group V: 10 mL of 2.5% NaOCl for 2 min (repeated 4 times). The apical thirds of the specimens were subjected to histological processing and 6-μm cross-sections were obtained and stained with hematoxylin-eosin. The specimens were examined under optical microscopy at ×40 magnification and the images were subjected to morphometric analysis using the Scion image-analysis software. The total area of root canal and the area with debris were measured in square millimeters. Analysis of variance showed no statistically significant difference (p>0.05) among the groups GI (2.39 ± 3.59), GII (2.91 ± 2.21), GIII (0.73 ± 1.36), GIV (0.95 ± 0.84) and GV (0.51 ± 0.22). In conclusion, the final irrigation protocols evaluated in this study using the Luer syringe presented similar performance in the removal of debris from the apical third of flattened root canals.

scholarly journals Adjunct use of mouth rinses with a sonic toothbrush accelerates the detachment of a Streptococcus mutans biofilm: an in vitro study

2020 ◽  
Author(s):  
Tatsuya Ohsumi ◽  
Shoji Takenaka ◽  
Yuuki Sakaue ◽  
Yuki Suzuki ◽  
Ryoko Nagata ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
In Vitro Study ◽  
Combination Group ◽  
Control Group ◽  
Mouth Rinse ◽  
Biofilm Thickness ◽  
Log Reduction ◽  
Mouth Rinses ◽  
Significant Difference

Abstract Background: The aim of this in vitro study was to examine the possible enhancement of the biofilm peeling effect of a sonic toothbrush following the use of an antimicrobial mouth rinse.Methods: The biofilm at a noncontact site in the interdental area was treated by sound wave convection with the test solution or by immersion in the solution. The biofilm peeling effect was evaluated by determining the bacterial counts and performing morphological observations. A Streptococcus mutans biofilm was allowed to develop on composite resin discs by cultivation with stirring at 50 rpm for 72 hours. The specimens were then placed in recesses located between plastic teeth and divided into an immersion group and a combination group. The immersion group was treated with phosphate buffer, Peridex™ mouth rinse or Listerine® mouth rinse. The combination group was treated with Peridex™ or Listerine® and a sonic toothbrush.Results: The biofilm thickness was reduced by approximately one-half compared with the control group. The combination treatment produced a 1 log reduction in the number of bacteria compared to the Listerine® immersion treatment. No significant difference was observed in the biofilm peeling effect of the immersion group compared to the control group.Conclusions: The combined use of a sonic toothbrush and a mouth rinse enhanced the peeling of the biofilm that proliferates in places that are difficult to reach using mechanical stress.

scholarly journals 160 IN VIVO-CULTURE OF BOVINE EMBRYOS: TRANSFER OF SEMEN PRE-INCUBATED OOCYTES, ZYGOTES AND 4 TO 8 CELL STAGE EMBRYOS INTO THE BOVINE OVIDUCT

2005 ◽  
Vol 17 (2) ◽  
pp. 231
Author(s):  
V. Havlicek ◽  
F. Wetscher ◽  
T. Huber ◽  
M. Gilles ◽  
D. Tesfaye ◽  
...  
Keyword(s):  
Embryo Development ◽  
Calf Serum ◽  
Bovine Embryos ◽  
Cell Stage ◽  
Group Iii ◽  
Group I ◽  
In Vivo Culture ◽  
Group Ii

Oviduct as well as oocyte and embryo development are subject to developmental changes which have crucial effects on the application of in vivo culture. The present study aimed at optimizing in vivo culture of IVP bovine embryos at different developmental stages in the bovine oviduct. Cumulus oocyte complexes (COC) were collected from slaughterhouse ovaries, matured in vitro for 22 h and assigned to four groups. In groups I and II, oocytes were pre-incubated for 3 to 4 h with 5 × 106 sperm/mL, and then immediately transferred to recipients, which had just completed ovulation (group I), or kept in vitro for a further 12 to 18 h and transferred to Day 1 synchronized recipients (group II). In groups III and IV, COC were subjected to standard IVF/IVC; then embryos were either transferred at the 4- to 8-cell stage on Day 3 into the oviducts of Day 3-synchronized recipients (group III) or kept in vitro for a further 4 to 5 days (group IV). Thirty-four 18- to 30-month-old temporary recipients were synchronized using a standard Ovsynch protocol. COC and embryos were transferred and re-collected by transvaginal endoscopy. COC or embryos were loaded into a 180° curved glass capillary, which was inserted via the infundibulum 5 to 8 cm deep into the ampulla ipsilateral to the CL. On recipient Day 7, a 90° curved metal canula served for tubal flushing prior to conventional uterine embryo flushing. Sixty mL of PBS containing 1% fetal calf serum were rinsed through the oviduct into the uterus and a further 400 mL of medium were finally used for flushing of the uterine horn and collected via an embryo filter. Embryo development was evaluated directly after flushing (Day 7) and on Day 8. For statistical analysis (ANOVA), the blastocyst rates (Days 7 and 8) in group III were related to COC corrected by the collection rate. In group I, 575 COC were transferred to 11 recipients and 420 (73%) were re-collected as oocytes or embryos. The blastocyst yields on Day 7 and Day 8 were 23% (97) and 25% (104), respectively. In group II, the transfer of 489 presumptive zygotes into 13 heifers resulted in only 175 re-collected (36%), of which 15% developed into blastocysts (Day 7: 26; Day 8: 27). Ten heifers (group III) served for in vivo culture of 643 embryos at the 4- to 8-cell stage. On Day 7, 568 (88%) embryos were flushed and 171 (30%) reached the blastocyst stage. A further 24 h culture in vitro finally resulted in 244 (42%) blastocysts. The complete in vitro production system delivered 13% (63/477) blastocysts on Day 7 and 34% (161/477) blastocysts on Day 8. The collection rates (P < 0.001) and the blastocyst rates on Day 7 (P < 0.05) and Day 8 (P < 0.001) differed significantly in all groups. The present data demonstrate that the developmental stage of transferred complexes has an influence on embryo recovery as well as an embryo development. This work was supported by Austrian BMBWK and BMLFUW (#1227).

scholarly journals Comparative Evaluation of Mixture of Calcium Hydroxide and Chlorhexidine, with Triple Antibiotic Paste and Combination of Calcium Hydroxide, Chlorhexidine, and Lycopene on Incidence of Interappointment Flare-up: An in vivo Study

2017 ◽  
Vol 1 (1) ◽  
pp. 10-14
Author(s):  
Priyanka S Bilgi ◽  
Nimisha C Shah ◽  
Jash Mehta
Keyword(s):  
Calcium Hydroxide ◽  
Comparative Evaluation ◽  
Rating Scale ◽  
Clinical Performance ◽  
Group Iii ◽  
Group I ◽  
Triple Antibiotic Paste ◽  
Significant Difference ◽  
Post Hoc

ABSTRACT Background The present study was undertaken to evaluate the most suitable intracanal medicament with the least incidence on Interappointment flare-up. Aim To clinically evaluate and compare the effect of combination of calcium hydroxide (CH) and chlorhexidine (CHX) with triple antibiotic paste and combination of CH, CHX, and lycopene on interappointment flare-up. Materials and methods A total of 36 patients requiring root canal treatment were selected according to the selection criteria and divided into three groups: Group I – CH and CHX, group II – triple antibiotic paste, and group III – CH, CHX, and lycopene. Access opening and biomechanical preparation of all samples, and intracanal medicaments were placed respectively. Subsequently, interappointment flare-up was assessed at 1, 2, 3, 7, and 14 days using verbal rating scale. Statistical analysis used One way analysis of variance test and Tukey's post hoc test. Results There was no statistically significant difference seen in the clinical performance of CH + CHX, triple antibiotic paste, and mixture of CH + CHX + lycopene at the end of 14 days in terms of interappointment flare-up. Conclusion All the intracanal medicaments were effective in reducing interappointment flare-up at the end of 14 days. How to cite this article Bilgi PS, Shah NC, Mehta J. Comparative Evaluation of Mixture of Calcium Hydroxide and Chlorhexidine, with Triple Antibiotic Paste and Combination of Calcium Hydroxide, Chlorhexidine, and Lycopene on Incidence of Interappointment Flare-up: An in vivo Study. Int J Clin Dent Res 2017;1(1):10-14.

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