scholarly journals Adjunct use of mouth rinses with a sonic toothbrush accelerates the detachment of a Streptococcus mutans biofilm: an in vitro study

2020 ◽  
Author(s):  
Tatsuya Ohsumi ◽  
Shoji Takenaka ◽  
Yuuki Sakaue ◽  
Yuki Suzuki ◽  
Ryoko Nagata ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
In Vitro Study ◽  
Combination Group ◽  
Control Group ◽  
Mouth Rinse ◽  
Biofilm Thickness ◽  
Log Reduction ◽  
Mouth Rinses ◽  
Significant Difference

Abstract Background: The aim of this in vitro study was to examine the possible enhancement of the biofilm peeling effect of a sonic toothbrush following the use of an antimicrobial mouth rinse.Methods: The biofilm at a noncontact site in the interdental area was treated by sound wave convection with the test solution or by immersion in the solution. The biofilm peeling effect was evaluated by determining the bacterial counts and performing morphological observations. A Streptococcus mutans biofilm was allowed to develop on composite resin discs by cultivation with stirring at 50 rpm for 72 hours. The specimens were then placed in recesses located between plastic teeth and divided into an immersion group and a combination group. The immersion group was treated with phosphate buffer, Peridex™ mouth rinse or Listerine® mouth rinse. The combination group was treated with Peridex™ or Listerine® and a sonic toothbrush.Results: The biofilm thickness was reduced by approximately one-half compared with the control group. The combination treatment produced a 1 log reduction in the number of bacteria compared to the Listerine® immersion treatment. No significant difference was observed in the biofilm peeling effect of the immersion group compared to the control group.Conclusions: The combined use of a sonic toothbrush and a mouth rinse enhanced the peeling of the biofilm that proliferates in places that are difficult to reach using mechanical stress.

scholarly journals Adjunct use of mouth rinses with a sonic toothbrush accelerates the detachment of a Streptococcus mutans biofilm: an in vitro study

2020 ◽  
Author(s):  
Tatsuya Ohsumi ◽  
Shoji Takenaka ◽  
Yuuki Sakaue ◽  
Yuki Suzuki ◽  
Ryoko Nagata ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
In Vitro Study ◽  
Vitro Study ◽  
Combination Group ◽  
Control Group ◽  
Mouth Rinse ◽  
Biofilm Thickness ◽  
Log Reduction ◽  
Significant Difference

Abstract Background : The aim of this in vitro study was to examine the possible enhancement of the biofilm peeling effect of a sonic toothbrush following the use of an antimicrobial mouth rinse. Methods: The biofilm at a noncontact site in the interdental area was treated by sound wave convection with the test solution or by immersion in the solution. The biofilm peeling effect was evaluated by determining the bacterial counts and performing morphological observations. A Streptococcus mutans biofilm was allowed to develop on composite resin discs by cultivation with stirring at 50 rpm for 72 hours. The specimens were then placed in recesses located between plastic teeth and divided into an immersion group and a combination group. The immersion group was treated with phosphate buffer or Listerine ® mouth rinse. The combination group was treated with Listerine ® and a sonic toothbrush. Results: The biofilm thickness was reduced by approximately one-half compared with the control group. The combination treatment produced a 1 log reduction in the number of bacteria compared to the Listerine ® immersion treatment. No significant difference was observed in the biofilm peeling effect of the immersion group compared to the control group. Conclusions: The combined use of a sonic toothbrush and a mouth rinse enhanced the peeling of the biofilm that proliferates in places that are difficult to reach using mechanical stress.

scholarly journals Adjunct use of mouth rinses with a sonic toothbrush accelerates the detachment of a Streptococcus mutans biofilm: an in vitro study

2020 ◽  
Author(s):  
Tatsuya Ohsumi ◽  
Shoji Takenaka ◽  
Yuuki Sakaue ◽  
Yuki Suzuki ◽  
Ryoko Nagata ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
In Vitro Study ◽  
Vitro Study ◽  
Combination Group ◽  
Control Group ◽  
Mouth Rinse ◽  
Biofilm Thickness ◽  
Log Reduction ◽  
Significant Difference

Abstract Background : The aim of this in vitro study was to examine the possible enhancement of the biofilm peeling effect of a sonic toothbrush following the use of an antimicrobial mouth rinse. Methods: The biofilm at a noncontact site in the interdental area was treated by sound wave convection with the test solution or by immersion in the solution. The biofilm peeling effect was evaluated by determining the bacterial counts and performing morphological observations. A Streptococcus mutans biofilm was allowed to develop on composite resin discs by cultivation with stirring at 50 rpm for 72 hours. The specimens were then placed in recesses located between plastic teeth and divided into an immersion group and a combination group. The immersion group was treated with phosphate buffer, Peridex™ mouth rinse or Listerine ® mouth rinse. The combination group was treated with Peridex™ or Listerine ® and a sonic toothbrush. Results: The biofilm thickness was reduced by approximately one-half compared with the control group. The combination treatment produced a 1 log reduction in the number of bacteria compared to the Listerine ® immersion treatment. No significant difference was observed in the biofilm peeling effect of the immersion group compared to the control group. Conclusions: The combined use of a sonic toothbrush and a mouth rinse enhanced the peeling of the biofilm that proliferates in places that are difficult to reach using mechanical stress.

scholarly journals PERBANDINGAN EFEKTIFITAS JUS LIDAH BUAYA DAN KLORHEXIDIN 0.06% TERHADAP JUMLAH KOLONI STREPTOCOCCUS MUTANS SALIVA ANAK DENGAN KARIES

2018 ◽  
Vol 5 (1) ◽  
pp. 54
Author(s):  
Shanty Chairani ◽  
Sri W Rais ◽  
Rani Purba ◽  
Amalia AH
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Aloe Vera ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Control Group ◽  
Mouth Rinse ◽  
Active Components ◽  
Control Group Design

Background: Aloe vera contains active components such as antrhaquinones,saponin, glucomannans, and acemannans that have been known for its antimicrobial properties. In vitro study showed that Aloe vera extract can inhibit the growth of Streptococcus mutans. Studies reported that children with caries have high level of salivary S. mutans which is the main pathogen in dental caries. The gold standard mouthrinse for dental caries is chlorhexidine gluconate, but it has several side effects. The objective of this study was to compare the effect between Aloe vera juice and 0.06% chlorhexidine mouth rinse on salivary S. mutans in children with caries.Methods: This experimental research with pretest and post-test control group design conducted on 30 children (6-12 years old) with number of carious teeth >4. Subjects divided into 2 groups: Aloe vera juice group and 0.06% chlorhexidine group, which instructed to rinse their mouth with 10 ml stated mouthwash, twice a day, during 7-day period. Unstimulated saliva was collected at baseline and after 7 days then inoculated on mitis salivarius bacitracin agar. S. mutans were counted and expressed in CFU/ml. Data were analyzed using paired t-test and independent t-test.Result: The result showed that a significant reduction in salivary S.mutanscounts was observed with Aloe vera juice mouth rinse and 0.06% chlorhexidine (p<0.05). The mean reduction of S. mutans counts was not statistically significantly between grup (p>0.05).Conclusion: These findings suggest that Aloe vera juice can reduce salivary S. mutans in children with caries so it can be considered as alternative mouthwash to prevent caries.

Comparative Study to Evaluate the Surface Detail Reproduction of Dental Stone after Immersion in Various Different Disinfectant Solutions, Under Stereomicroscope 10 X Magnification –An In-Vitro Study

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Rathika Rai ◽  
M. A. Easwaran ◽  
K. T. Dhivya
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Control Group ◽  
Immersion Method ◽  
Significant Difference ◽  
Surface Irregularities ◽  
Disinfectant Solution ◽  
Surface Detail ◽  
Group B

Aim: To evaluate the surface detail reproduction of dental stone this is immersed in different disinfectant solution and studied under stereomicroscope. Methodology: Total number of 30 specimens of dental stone (Type III) were made with measurements of 1.5cm diameter and 1cm height .This samples are divided in to 3 groups group A,B,C. were A is immersed in Distilled water which was taken as control group ;B is immersed in 2% Glutaraldehyde and C is immersed in 5%sodium hypochlorite. Each specimen were immersed in the disinfectant solution for 15 minutes and dried under room temperature for 24 hrs. After 24 hrs each specimens are studied under stereomicroscope for surface details. Result: The results showed no significant difference in the surface irregularities and porosities for a group 1 and group 2 except group 3 which showed significant increase in the porosities, surface irregularities and erosions after disinfection with 5% NaHOCl by immersion method. Conclusion: The surface detail reproduction capacity of die stone was adversely affected when 5% Sodium hypochlorite was used as disinfectant solution when compare d to control group and 2% Glutaraldehyde

scholarly journals In vitro study of human osteoblast proliferation and morphology on orthodontic mini-implants

2015 ◽  
Vol 85 (6) ◽  
pp. 920-926 ◽  
Author(s):  
Ricardo Carvalho Bueno ◽  
Roberta Tarkany Basting
Keyword(s):  
Chemical Composition ◽  
In Vitro Study ◽  
Chemical Components ◽  
Control Group ◽  
Osteoblast Proliferation ◽  
Mini Implants ◽  
Significant Difference ◽  
Implant Anchorage ◽  
Positive Effect

ABSTRACT Objective:  To evaluate the proliferation and morphology of human osteoblasts cultured on two brands of mini-implants after 24, 48, and 72 hours, in addition to the chemical composition found on their surface. Materials and Methods:  Two brands of mini-implant (Morelli and Neodent) were evaluated; polystyrene was used as a control group (n  =  3). Osteoblasts were cultured on the surface of sterilized mini-implants in a CO2 incubator at different time periods (24, 48, and 72 hours). Osteoblast proliferation was quantified by scanning electron microscopy using up to 5000× magnification, and cell morphology was analyzed by a single observer. For the chemical analysis, spectroscopy X-ray fluorescence was used to identify and quantify chemical components on the surface of the mini-implants. Results:  Two-way ANOVA showed no significant interaction between the factors studied (P  =  0.686). A Tukey test revealed no significant difference in osteoblast proliferation between the mini-implants at all studied periods; however, a difference in cell proliferation was detected between the Neodent and the control group (P  =  .025). For all groups, time had a direct and positive effect on osteoblast proliferation (P &lt; .001). The significant elements present in both brands of mini-implants were titanium, aluminum, vanadium, and iron. Conclusions:  Osteoblast proliferation was present on the mini-implants studied, which increased over time; however, no significant difference between brands was observed. No difference was seen between the mini-implants evaluated in terms of chemical composition. Cell adhesion after 72 hours suggests that areas of bone remodeling can be achieved, thus initiating the process of mini-implant anchorage.

scholarly journals The Effect of the Bioactive Glass and the Er:YAG Laser on the Remineralization of the Affected Dentin: A Comparative In Vitro Study

2020 ◽  
Vol 11 (2) ◽  
pp. 160-166
Author(s):  
Mohammad Javad Moghaddas ◽  
Horieh Moosavi ◽  
Sara Yaghoubirad ◽  
Nasim Chiniforush
Keyword(s):  
Bioactive Glass ◽  
In Vitro Study ◽  
Control Group ◽  
Dentinal Tubule ◽  
Glass Ionomer ◽  
Significant Difference ◽  
Post Hoc ◽  
Caries Lesions ◽  
Cavity Floor

Introduction: The purpose of this study was to compare the effect of the bioactive glass, the glass ionomer, and the Erbium YAG laser as liners on the remineralization of the affected dentin. Methods: The present study was conducted on 64 healthy extracted human molars divided into 4 groups, 1 control group and 3 experimental groups. After artificially inducing dentinal caries lesions, each of the experimental groups was applied to the cavity floor and then restored with a composite. The samples were stored after thermocycling in an incubator for two months. Finally, the hardness of the cavity floor was measured at 3 depths of 20, 50 and 100 μm by the Vickers microhardness tester. The dentin conditions underneath the liners were also evaluated with FESEM. Statistical analysis was performed by two-way ANOVA and the post-hoc Games-Howell test (P<0.05). Results: Among the groups, the lowest microhardness value was in the control group (P<0.05) except at a depth of 100 μm; therefore, there was no significant difference between the control group and the bioactive glass (P>0.05). The laser group had the highest microhardness value, which was significantly different from the control group (P<0.05). There was a significant difference between the laser and bioactive glass (P<0.05), except at a depth of 20 μm. The laser and glass ionomer had only a significant difference at a depth of 100 μm (P<0.05). The microhardness value induced by glass ionomer was higher than bioactive glass, which in no depth was significant (P>0.05). Partial dentinal tubule occlusion was observed with FESEM in each of the experimental groups as compared to the control group. Conclusion: The microhardness values were higher in all groups than in the control group. The laser might be more successful in remineralization than the other ones.

scholarly journals Milled versus moulded mock-ups based on the superimposition of 3D meshes from digital oral impressions: a comparative in vitro study in the aesthetic area

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Francesca Cattoni ◽  
Giulia Teté ◽  
Alessandro Mauro Calloni ◽  
Fabio Manazza ◽  
Giorgio Gastaldi ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Test Group ◽  
In Vitro Study ◽  
Mean Value ◽  
Digital Impression ◽  
Control Group ◽  
Minimal Invasiveness ◽  
Significant Difference ◽  
The Aesthetic

Abstract Background Aesthetic porcelain veneers proved to be a long-term reliable prosthetic solution, ensuring minimal invasiveness. The use of veneers requires an adhesive cementation technique, so maintaining as much enamel as possible is to ensure lasting success. A diagnostic mock-up is a key tool that allows a preview of the outcome of the aesthetic restoration: it is obtainable both in an analog and digital way. With the recent developments in impression technology and the ever so fast growing use of CAD-CAM technologies it is useful to understand the pros and cons of either one of these techniques (analog and digital) in order to identify the easier and more convenient workflow in aesthetic dentistry. Methods After taking pictures and impressions of the dental arcs of a patient in need of aesthetic rehabilitation, 52 resin models were produced and a digital drawing of the smile was outlined. Both an analog and a digital wax-up were obtained from two of the 52 models: the latter was obtained using digital impressions and a dedicated software. The analog wax-up was then used to produce 25 matrices that have later been used to mould 25 resin mock-ups using a traditional moulding protocol (Control Group - CG). The digital wax-up was used to mill 25 PMMA mock-ups. Each mock-up, both milled and moulded (total 50), was then laid on the other 50 resin models as a digital impression of it was taken. The STL. files of the milled mock-ups were compared with the 3D CAD wax-up made using a specific software. The STL. files of the analog printed mock-ups were compared with the traditional wax-up design. A statistical analysis was carried out to evaluate the difference between the groups. Results The statistical analysis showed a significant difference (P > 0.01) between the mean value of the distance between the points of the overlapping STL. meshes in GC (0.0468 mm) and in TG (Test Group - TG) (0,0109 mm). Conclusions The study showed a difference in accuracy between traditional moulded and milled mock-ups compared to their original wax-up. The data analysis reports that the digital method allows for greater accuracy. Within the limitations of this study, a fully digital workflow is to considered more reliable when it come to creating an esthetic mockup: the digital procedure has been shown to be more accurate than the one made manually which is much more operator dependent and it brings an increase to the chance of error, and that could ultimately affect the final result.

scholarly journals THE EFFECT OF CURCUMA XANTHORRHIZA ETHANOL EXTRACT ON THE VIABILITY OF STREPTOCOCCUS MUTANS AND AGGREGATIBACTER ACTINOMYCETEMCOMITANS (DENTAL BIOFILM RESEARCH: IN VITRO STUDY)

2017 ◽  
Vol 10 (17) ◽  
pp. 30
Author(s):  
Royan Diana ◽  
Hedijanti Joenoes ◽  
Ariadna A Djais
Keyword(s):  
Streptococcus Mutans ◽  
In Vitro Study ◽  
Ethanol Extract ◽  
Single Species ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Vitro Study ◽  
Dental Biofilm ◽  
Significant Difference ◽  
Curcuma Xanthorrhiza

Objective: This study aimed to compare the effect of Curcuma xanthrorrhiza ethanol extract to the viability of Streptococcus mutans and Aggregatibacter  actinomycetemcomitans using single- and dual-species biofilm at different phases of formation.Methods: Biofilm models were incubated for 4, 12, and 24 hrs, then exposed to the extract at a concentration of 0.525%.Results: The viability of the single-species S. mutans biofilm was low (p<0.05), and no significant difference (p>0.05) was found between singlespeciesA. actinomycetemcomitans and dual-species biofilm.Conclusions: Curcuma xanthorrhiza ethanol extract is more effective for decreasing the viability of single-species S. mutans biofilm.

scholarly journals Comparative evaluation of force decay pattern in orthodontic active tiebacks exposed to five different mouth rinses: An in vitro Study

2020 ◽  
Vol 14 (4) ◽  
pp. 244-249
Author(s):  
Amir Hossein Mirhashemi ◽  
Atefe Saffar Shahroudi ◽  
Keyvan Shahpoorzadeh ◽  
Niloofar Habibi Khameneh
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Control Group ◽  
Mouth Rinses ◽  
Force Decay ◽  
Force Relaxation ◽  
Decay Pattern ◽  
Initial Force ◽  
And Control

Background. This study compared the force decay pattern of two different orthodontic active tiebacks (ATBs) exposed to five different commercially available mouth rinses. Methods. In this in vitro study, 90 transparent ATBs and 90 gray ATBs were divided into six groups; one was the control group, and the others were exposed to one of these mouth rinses twice a day for 60 seconds: Listerine, chlorhexidine, Orthokin, Persica, and fluoride. The initial force of each ATB was 250 g at a 24-mm extension. The force of ATBs was measured on days 1, 7, 14, and 28 using a digital gauge. Results. The highest percentage of force loss was observed between days 14 and 28 (P<0.05). At the end of the study, the Persica group exhibited the highest force degradation in both ATB types. In the transparent ATBs, it was followed by Orthokin, Listerine, fluoride, chlorhexidine, and control groups, respectively. In the gray ATBs, Orthokin, chlorhexidine, control, Listerine, and fluoride groups exhibited the highest force decay in descending order. In some groups, the differences between transparent and gray ATBs were significant. In the control group, the force of transparent ATB was significantly higher than gray ones on days 7 and 14 but not significantly after four weeks. Conclusion. ATBs’ force degradation could be exacerbated by the use of some mouth rinses. There were some differences between force relaxation patterns of transparent and gray ATBs. The data could be beneficial in choosing appropriate O-rings for making ATBs.

scholarly journals Effect of 5% Calcium Hypochlorite on Mechanical Properties of Root Dentin: An in vitro Study

2016 ◽  
Vol 1 (2) ◽  
pp. 56-59
Author(s):  
Natanasabapathy Velmurugan ◽  
Poornima Reddy ◽  
Suma Balla ◽  
Sandhya S Raghu ◽  
Garlapati T Gupta ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Flexural Strength ◽  
Modulus Of Elasticity ◽  
In Vitro Study ◽  
Chlorine Concentration ◽  
Control Group ◽  
Calcium Hypochlorite ◽  
Significant Difference ◽  
Root Dentin

ABSTRACT Introduction The aim of this study was to compare in vitro the effect of 5% calcium hypochlorite [Ca(OCl)2] and 5% sodium hypochlorite (NaOCl) on flexural strength and modulus of elasticity of root dentin. Materials and methods The available chlorine concentration of each solution was determined using iodometric titration. Standardized planoparallel dentin bars (n= 20) were divided into two test groups and one control group. The control, group 1, consisted of dentin bars stored in normal saline. The dentin bars in the two test groups were treated by exposure to following solutions: Group 2 to 5% Ca(OCl)2; and group 3 to 5% NaOCl. All the three test solutions were changed once in 15 minutes for 30 minutes. The dentin bars were then loaded to failure using three-point bend test. Results Available chlorine concentration was 64% in both the test solutions. There was a significant reduction in the flexural strength of 5% NaOCl group compared to 5% Ca(OCl)2-treated ones. A significant difference in modulus of elasticity was observed between the test groups and the control groups and also between the 5% Ca(OCl)2 and 5% NaOCl groups. Conclusion Within the limitations of this study, 5% NaOCl reduced the flexural strength and modulus of elasticity of root dentin bars more when compared to 5% Ca(OCl)2. How to cite this article Reddy P, Balla S, Raghu SS, Velmurugan N, Gupta GT, Sahoo HS. Effect of 5% Calcium Hypochlorite on Mechanical Properties of Root Dentin: An in vitro Study. J Oper Dent Endod 2016;1(2):56-59.

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