scholarly journals Biosynthesis of Gold Nanoparticles by Essential Oil of Diplotaxis Acris Characterization and Antimicrobial Activities

2021 ◽  
Vol 37 (2) ◽  
pp. 405-412
Author(s):  
Mohamed Habib Oueslati ◽  
Lotfi Ben Tahar ◽  
A. Khuzaim Alzahrani ◽  
Jamith Basha ◽  
Omar H. Abd Elkader
Keyword(s):  
Essential Oil ◽  
Average Particle Size ◽  
Antimicrobial Activities ◽  
Nano Particles ◽  
Average Particle ◽  
Tem Analysis ◽  
Stabilizing Agents ◽  
E Coli ◽  
Ft Ir ◽  
Plausible Scheme

The present work reports a green biosynthesis of gold nano particles (EO-AuNPs) using an essential oil (EO) as a reducing agent of the Au(III) in HAuCl4. The EO was extracted by hydro-distillation from Diplotaxis acris flowers. A total of 16 compounds were detected from the EO oil by using GC–MS and 5-methylsulfanylpentanenitrile was identified as the major component (73.60 %). The biosynthesized EO-AuNPs were characterized performing UV–Vis, IR,XRD and TEM analyses.The UV-Vis revealed the typical features of surface plasmon resonance (SPR) of AuNPs at ~526 nm. The FT-IR spectrum of the biosynthesized nano particles exhibited the features of the nitrile (-C≡N) functional group indicating that the -C≡N-bearing EO components are likely acting as reducing and stabilizing agents for the formation of EO-AuNPs. The plausible scheme of EO-AuNPsformation was proposed.The TEM analysis showed that the EO- AuNPs were almost spherical in shape with an average particle size of 12.7 nm. In addition, the antimicrobial activity was carried out by diffusion of agar wells method. The results proved that the EO-AuNPs displayed a potential antimicrobial against gram negative strains, with a maximum zone of inhibition of 16 mm for E. coli at a concentration of 100 µg / ml.

scholarly journals Development and Characterization of Polyphenon 60 and Caffeine Microemulsion for Enhanced Antibacterial Activity

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Sonal Gupta ◽  
Rakhi Bansal ◽  
Javed Ali ◽  
Reema Gabrani ◽  
Shweta Dang
Keyword(s):  
Antibacterial Activity ◽  
Mammalian Cells ◽  
Average Particle Size ◽  
Natural Compounds ◽  
Average Particle ◽  
Tem Analysis ◽  
E Coli ◽  
Vero Cell Line ◽  
Effective Delivery ◽  
The Individual

Green tea catechins and caffeine have exhibited antibacterial activity; however, their use is limited by lack of stability and effective delivery systems. Polyphenon 60 (P60) and caffeine were encapsulated in a single microemulsion (ME) formulation with an objective to lower the minimum inhibitory concentrations (MICs) of the individual agents against selected pathogens (S. epidermidisandE. coli). Combination of two natural compounds would advocate two different mechanisms on the bacterial growth thereby providing for better antibacterial activity. Thermodynamically stable ME was developed and characterized with an average particle size of 17.58 nm, further confirmed by TEM analysis. Antibacterial studies included chequerboard microdilution assay to determine the MIC and fractional inhibitory concentration (FIC) of both the natural compounds individually and in combination. MIC and FIC results indicated that the combination of the above two natural compounds was proficient in lowering the MICs of individual agents. Results of DPPH assay indicated that ME system preserved the long term antioxidative potential of P60 and caffeine. The cytotoxicity of the optimized formulation on Vero cell line by MTT assay was found to be nontoxic to mammalian cells.

scholarly journals Variation of Alkali Concentration and Temperature: Its Effect on the Morphology of ZnO Nanoparticles Synthesized via Solvothermal Technique

2021 ◽  
Vol 411 ◽  
pp. 3-15
Author(s):  
Boon Siong Wee ◽  
Eric Kwabena Droepenu ◽  
Suk Fun Chin ◽  
Kuan Ying Kok ◽  
Woei Ting
Keyword(s):  
Electron Microscopy ◽  
Particle Size ◽  
Average Particle Size ◽  
Alkali Concentration ◽  
Average Particle ◽  
Zno Nanostructures ◽  
Tem Analysis ◽  
Vis Spectroscopy ◽  
Ft Ir ◽  
Concentration Ratios

This study reports on synthesis of ZnO nanostructures using Zinc chloride (ZnCl2) as precursors and Potassium hydroxide (KOH) as alkaline source in a solvothermal process with varying molar concentrations (Zn2+/OH-) of 1:1, 1:3 and 1:5 for temperatures of 30 °C and 50 °C. The synthesized nanostructures were characterized by X-Ray Diffraction (XRD), Field Emission Scanning Electron Microscopy (FE-SEM), Transmission Electron Microscopy (TEM), Fourier Transform Infrared (FT-IR) Spectroscopy, and Ultraviolet Visible (UV-Vis) spectroscopy. ZnO nanostructures synthesized at lower ratios (1:1) exhibited wurtzite hexagonal shapes. However, as the concentration ratios increases in both cases, spherical structures were formed with the emergence of some rod-like structures dominating, and finally aggregated to form flower-like structures at 30 °C temperature. The average crystallite size for nanostructures from XRD (30-50 °C) were in the range 15-21 nm whereas the average particle size from TEM analysis (30-50 °C) were in the range 39-76 nm. Increase in temperature and molar concentration of the alkaline source generally decreased the crystallite and particle size of the as well as a decrease in the wavelength of ZnO nanostructures as a result of blue-shifting of the absorption peak. FT-IR spectra of ZnO NSs prepared from concentration ratios of Zn2+: OH- (1:1, 1:3 and 1:5) at 30 °C and 50 °C showed characteristic peak bands at 461-467 cm-1 and 460-462 cm-1 respectively.

scholarly journals Synthesis and characterization of ternary Pt-alloy nano particles for hydrogen fuel cell application

2020 ◽  
Vol 12 (1) ◽  
pp. 419-425
Author(s):  
Alhassan Kabiru Usman ◽  
Kamal Danazumi
Keyword(s):  
Electron Microscope ◽  
Average Particle Size ◽  
Carbon Support ◽  
Peak Position ◽  
Reduction Reaction ◽  
Nano Particles ◽  
Alloy Formation ◽  
Average Particle ◽  
Hydrogen Fuel Cell ◽  
Tem Analysis

A novel ternary PtM2 nano structures was prepared using simple salvothermal process. The alloy formation has been ascertained using different characterizations techniques such as X-ray diffraction, Tunneling electron microscope TEM, Scanning electron microscope SEM, EDX. The XRD pattern reveals that, the peak position was shifted to higher 2𝜃 angle specifying the presence of Co and Ni into the lattice to form an ordered L10 phase with a reduced Pt-Pt distance. The thickness of the alloy was found to be ~4.3nm and interplaner distance of ~0.23nm which correlate with TEM analysis with an average particle size ~20nm and distribution of the catalyst on carbon support was also investigated by TEM images. SEM was used to obtain the surface morphology of the synthesized catalyst as well the amount of metallic loaded from EDX. Key words; Platinum, Cobalt, Nickel on Carbon Support (PCN/C), Platinum, Cobalt and Nickel for 8 hour (PCN-8h), Commercial Platinum on Carbon Support (Pt/C), Oxidation Reduction Reaction (ORR), PEMFC, SEM, EDX, TEM, XRD.  

scholarly journals Green approach in gold, silver and selenium nanoparticles using coffee bean extract

2020 ◽  
Vol 5 (1) ◽  
pp. 761-767
Author(s):  
Reiyhaneh Abbasian ◽  
Hoda Jafarizadeh-Malmiri
Keyword(s):  
Spherical Shape ◽  
Coffee Bean ◽  
Hydrothermal Conditions ◽  
Ag Nps ◽  
Au Nps ◽  
Stabilizing Agents ◽  
E Coli ◽  
Green Approach ◽  
Gold Silver ◽  
Ft Ir

AbstractGreen fabrication of metal nanoparticles (NPs), using natural reducing and stabilizing agents existed in plants and their derivatives, due to their unique properties, has gained more attention. The present study focuses on the synthesis of gold (Au), silver (Ag) and selenium (Se) NPs using coffee bean extract under hydrothermal conditions (1.5 atm and 121°C, for 15 min). Coffee bean extract obtained in 2 h processing using Clevenger apparatus and Fourier transform-infrared (FT-IR) spectroscopy indicated five highlighted peaks, namely, hydroxyl, amide, aromatic, alkane and ring groups. Dynamic light scattering analysis revealed that among three different NPs formed, fabricated Ag NPs had small particle size (153 nm) and high zeta potential value (16.8 mV). However, synthesized Au NPs had minimum polydispersity index (0.312). Results also indicated that fabricated Au, Se and Ag NPs had low antioxidant activity with values of 9.1, 8.9 and 8.7%, respectively. Morphological and antibacterial activity assessments, demonstrated that synthesized Ag, Au and Se NPs had spherical shape and high bactericidal activity against E. coli and S. aurous. Obtained results indicated that the synthesized NPs, can be utilized in various areas.

scholarly journals Synthesis of Gold Nanoparticles by Using Green Machinery: Characterization and In Vitro Toxicity

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 808
Author(s):  
Ahmed Al Saqr ◽  
El-Sayed Khafagy ◽  
Ahmed Alalaiwe ◽  
Mohammed F. Aldawsari ◽  
Saad M. Alshahrani ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Average Particle Size ◽  
In Vitro Cytotoxicity ◽  
In Vitro Toxicity ◽  
Cervical Cancer Cell Line ◽  
Average Particle ◽  
Anticancer Activities ◽  
Tem Analysis ◽  
Benincasa Hispida

Green synthesis of gold nanoparticles (GNPs) with plant extracts has gained considerable interest in the field of biomedicine. Recently, the bioreduction nature of herbal extracts has helped to synthesize spherical GNPs of different potential from gold salt. In this study, a fast ecofriendly method was adopted for the synthesis of GNPs using fresh peel (aqueous) extracts of Benincasa hispida, which acted as reducing and stabilizing agents. The biosynthesized GNPs were characterized by UV–VIS and Fourier transform infrared spectroscopy, transmission electron microscopy (TEM), and dynamic light scattering. In addition, the in vitro antibacterial and anticancer activities of synthesized GNPs were investigated. The formation of gold nanoparticles was confirmed by the existence of a sharp absorption peak at 520 nm, corresponding to the surface plasmon resonance (SPR) band of the GNPs. TEM analysis revealed that the prepared GNPs were spherical in shape and had an average particle size of 22.18 ± 2 nm. Most importantly, the synthesized GNPs exhibited considerable antibacterial activity against different Gram-positive and Gram-negative bacteria. Furthermore, the biosynthesized GNPs exerted remarkable in vitro cytotoxicity against human cervical cancer cell line, while sparing normal human primary osteoblast cells. Such cytotoxic effect was attributed to the increased production of reactive oxygen species (ROS) that contributed to the damage of HeLa cells. Collectively, peel extracts of B. hispida can be efficiently used for the synthesis of GNPs, which can be adopted as a natural source of antimicrobial and anticancer agent.

scholarly journals Photoluminescences properties of lanthanum-silver co-doped ZnO nano particles

2018 ◽  
Vol 4 (4) ◽  
pp. 135-141 ◽  
Author(s):  
V. Porkalai ◽  
B. Sathya ◽  
D. Benny Anburaj ◽  
G Nedunchezhian ◽  
S. Joshua Gnanamuthu ◽  
...  
Keyword(s):  
Zno Nanoparticles ◽  
Zinc Acetate Dihydrate ◽  
Average Particle Size ◽  
Semiconductor Nanoparticles ◽  
Nano Particles ◽  
Average Particle ◽  
Rare Earth Ion ◽  
X Ray ◽  
Doped Zno ◽  
Co Doped

Recently, transition metal (TM) and rare earth ion doped II–VI semiconductor nanoparticles have received much attention because such doping can modify and improve optical properties of II–VI semiconductor nanoparticles by large amount. In this study, undoped, La doped and La+Ag co-doped ZnO nano particles have been successfully synthesized by sol-gel method using the mixture of Zinc acetate dihydrate and ethanol solution. The powders were calcinated at 600 °C for 2 h. The effect of lanthanum and lanthanum-silver incorporation on the structure, morphology, optical and electrical conductivity were examined by X-ray diffraction (XRD), Scanning Electron Microscope (SEM), Energy Dispersive X-ray Absorption (EDAX), Fourier transform infrared spectroscopy (FTIR), UV and Photo Luminescence (PL) Characterization. The average particle size of the synthesized ZnO nanoparticles is calculated using the Scherrer formula and is found to be of less than 20 nm. Luminescences properties were found to be enhanced for the La and La+Ag co-doped ZnO nanoparticles.

scholarly journals Effect of Nanosized Colloidal Copper on Cotton Fabric

2010 ◽  
Vol 5 (3) ◽  
pp. 155892501000500 ◽  
Author(s):  
D.P. Chattopadhyay ◽  
B.H. Patel
Keyword(s):  
Particle Size ◽  
Cotton Fabric ◽  
Colloidal Solution ◽  
Chemical Reduction ◽  
Average Particle Size ◽  
Particle Size Analysis ◽  
Nano Particles ◽  
Size Analysis ◽  
Average Particle ◽  
Sem Images

This research deals with the synthesis of nanosized copper as colloidal solution and its application to cotton fabric. Copper nano colloids were prepared by chemical reduction of copper salt using sodium borohydride as reducing agent in presence of tri-sodium citrate. The size and size distribution of the particles were examined by particle size analyzer and the morphology of the synthesized particles was examined by SEM and AFM techniques. X-ray fluorescence spectroscopy detected the presence of copper in the treated fabric. The results of particle size analysis showed that the average particle size varied from 60 nm to 100 nm. The nano copper treated cotton was subjected to soil burial test for the assessment of its resistance towards microbial attack. SEM images of treated fabric indicate copper nano particles are well dispersed on the surface of the specimens. The treatments of nano copper colloidal solution on cotton not only improve its antimicrobial efficiency but also influenced the tensile strength of the fabric sample positively. The treatment was found to enhance the color depth and fastness properties of direct dyed cotton fabric samples.

scholarly journals Investigation on room temperature photoluminescence of pure and aluminum doped zinc oxide nanoparticles

2015 ◽  
Vol 33 (1) ◽  
pp. 205-212 ◽  
Author(s):  
N. Srinivasan ◽  
J.C. Kannan
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Average Particle Size ◽  
Hexagonal Wurtzite Structure ◽  
Spectral Studies ◽  
Oxide Nanoparticles ◽  
Average Particle ◽  
Ir Studies ◽  
Aluminum Doped Zinc Oxide ◽  
Ft Ir

AbstractPure and aluminum doped zinc oxide nanoparticles were prepared by soft chemical method. The prepared nanoparticles were characterized by XRD, SEM-EDAX, UV-Vis, PL and FT-IR studies. XRD patterns revealed that the nanoparticles were crystallized in hexagonal wurtzite structure with an average particle size of 19 nm to 26 nm. The surface morphology was explored using SEM micrographs. The incorporation of aluminum was confirmed by EDAX and FT-IR studies. The band gaps of the particles were found from 3.48 eV to 3.53 eV through UV-Vis spectral studies. The defect related mechanism was investigated using PL measurements. The chemical functional groups in FT-IR spectra proved the formation of pure and aluminum doped zinc oxide nanoparticles.

scholarly journals Antibacterial Shoe Insole-Coated CuO-ZnO Nanocomposite Synthesized by the Sol-Gel Technique

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Nguyen Lam Uyen Vo ◽  
Thi Thuy Van Nguyen ◽  
Tri Nguyen ◽  
Phung Anh Nguyen ◽  
Van Minh Nguyen ◽  
...  
Keyword(s):  
Inhibitory Concentration ◽  
Antibacterial Effect ◽  
Sol Gel ◽  
Average Particle Size ◽  
Point Of Zero Charge ◽  
Average Particle ◽  
E Coli ◽  
High Antibacterial Activity ◽  
Gel Technique ◽  
Shoe Insoles

In this study, CuO-ZnO composite was synthesized via the sol-gel method using oxalic acid to form the medium complex and its applications in antibacterial have been conducted with B. cereus, E. coli, S. aureus, Salmonella, and P. aeruginosa. Then, nanopowder of CuO-ZnO was coated on shoe insoles and their antibacterial effect with S. aureus was tested. The nanocomposite products were characterized by XRD, XPS, SEM, TEM, and UV-Vis. The results showed that the CuO-ZnO composite has the average particle size in a range of 20-50 nm, the point of zero charge of 7.8, and the bandgap of 1.7 eV. XPS result shows the composite structure with Cu2+ in the product. The minimum inhibitory concentration (MIC) of CuO-ZnO nanocomposite was 0.313 mg·mL-1 for S. aureus and Samonella, 0.625 mg·mL-1 for E. coli, and 5 mg·mL-1 for B. cereus and P. aeruginosa. The shoe insoles coated with 0.35 wt.% of CuO-ZnO nanocomposite also had high antibacterial activity against S. aureus, and this antibacterial nanocomposite was implanted durably on the surface of the shoe insoles.

scholarly journals Green synthesis of Ag, Zn and Cu nanoparticles from aqueous extract of Spondias mombin leaves and evaluation of their antibacterial activity

2020 ◽  
Vol 21 (2) ◽  
pp. 106-113
Author(s):  
D.K. Adeyemi ◽  
A.O. Adeluola ◽  
M.J. Akinbile ◽  
O.O. Johnson ◽  
G.A. Ayoola
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Green Synthesis ◽  
Antimicrobial Activities ◽  
Cu Nanoparticles ◽  
E Coli ◽  
Spondias Mombin ◽  
Ft Ir ◽  
Uv Visible

Background: Nanotechnology offers an advantage as a green route for synthesis of metal nanoparticles (NPs) with plant extracts as capping agent. Spondias mombin is a fruit-bearing tree and its leaf extracts have been reported to possess anxiolytic, hypoglycaemic, antiepileptic, antipsychotic, sedative, antioxidant, and antimicrobial properties. The objective of the study is to determine the antibacterial potential of a simple non-toxic product of green synthesis of metallic (Ag, Zn and Cu) nanoparticles using the leaf of Spondias mombin aqueous extracts (SMAE) as a reducing and capping agents of the metal ions.Methodology: Nanoparticles were characterized by UV visible spectrophotometeric analysis, Fourier Transform Infra-Red (FT-IR) spectrophotometer and scanning electron microscope (SEM). Antimicrobial activities of synthesized NPs against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were determined by agar well diffusion technique.Results: The synthesized NPs varied in colour from dark brown to green and appears dominantly spherical, occasionally rod or triangular shaped with size ranging from 65-90 nm. UV spectroscopy absorption spectra of Ag, Zn and Cu NPs had absorbance peak at 267, 262 and 765 nm respectively. FT-IR spectrometry of Zn NP, Cu NP, and SMAE gave wave number ranging from 895.71-3320.67, 747.02-3225.45 and 658.25-3674.49 respectively. FT-IR analysis showed that SMAE acted as reducing and stabilizing agent while the NPs exhibited lower energy absorption band when compared to the plant extract. The NPs demonstrated higher antimicrobial activities against S. aureus than Ps. aeruginosa and E. coli. The antimicrobial activity was higher with copper NP than Ag and Zn NPs, and also higher than SMAE.Conclusion: The result from this study presents an indication for an alternative means for development of novel antimicrobial agents for clinical and biotechnological applications.Keywords: synthesis, nanoparticles, FT-IR, UV-visible spectrophotometry, antibacterial activitiesFrench Title: Synthèse verte de nanoparticules d'Ag, de Zn et de Cu à partir d'extrait aqueux de feuilles de Spondias mombin et évaluation de leur activité antibactérienneContexte: La nanotechnologie offre un avantage en tant que voie verte pour la synthèse de nanoparticules métalliques (NP) avec des extraits de plantes comme agent de coiffage. Spondias mombin est un arbre fruitier et ses extraits de feuilles possèdent des propriétés anxiolytiques,  hypoglycémiques, antiépileptiques, antipsychotiques, sédatives,  antioxydantes et antimicrobiennes. L’objectif de l’étude est de déterminer le potentiel antibactérien d’un simple produit non toxique de synthèse verte de nanoparticules métalliques (Ag, Zn et Cu) à l’aide de la feuille d’extraits aqueux de Spondias mombin (SMAE) en tant qu’agent réducteur et coiffant de la ions métalliques.Méthodologie: Les nanoparticules ont été caractérisées par analyse  spectrophotométrique UV visible, spectrophotomètre à transformée de Fourier infrarouge (FT-IR) et microscope électronique à balayage (MEB). Lesactivités antimicrobiennes de NP synthétisées contre Escherichia coli, Staphylococcus aureus et Pseudomonas aeruginosa ont été déterminées par une technique de diffusion sur puits d’agar.Résultats: Les NP synthétisés ont une couleur allant du brun foncé au vert et apparaissent principalement sphériques, parfois en bâtonnets ou en triangles, avec des tailles allant de 65 à 90 nm. Les spectres  d'absorption par spectroscopie UV des NP Ag, Zn et Cu présentaient des pics  d'absorbance à 267, 262 et 765 nm respectivement. La spectrométrie FT-IR de Zn NP, Cu NP et SMAE a donné un nombre d'onde allant de 895,71 à 3320,67, 747,02 à 3225,45 et 658,25 à 3674,49 respectivement. L'analyse FT-IR a montré que le SMAE agissait en tant qu'agent réducteur et  stabilisant, alors que les NP présentaient une bande d'absorption d'énergie inférieure à celle de l'extrait de plante. Les NP ont démontré des activités antimicrobiennes plus élevées contre S. aureus que Ps. aeruginosa et E. coli. L'activité antimicrobienne était plus élevée avec les NP en cuivre que dans les NP Ag et Zn, et également supérieure à celle du SMAE.Conclusion: le résultat de cette étude présente une indication d'un autre moyen de développement de nouveaux agents antimicrobiens pour des applications cliniques et biotechnologiques.Mots-clés: synthèse, nanoparticules, FT-IR, spectrophotométrie UV-visible, activités antibactériennes 

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