Mermaid: A Shellfish Sanitation Model Providing Additional Metrics for the Classification of Shellfish Growing Areas of Virginia (USA), Managed by the Direct Rule Method

2017 ◽  
Vol 33 (6) ◽  
pp. 825-839 ◽  
Author(s):  
Fred S. Conte ◽  
Abbas Ahmadi

Abstract. The Virginia Department of Shellfish Sanitation (VDSS) manages shellfish growing areas using the Direct Rule method, by directly comparing the Geometric Mean and Estimated 90th Percentile of fecal coliform concentrations to the U.S. National Shellfish Sanitation Program (NSSP) standard. The agency closes the area to harvest if fecal coliform concentrations exceed the NSSP limit and the area is not reopened until concentrations fall below the NSSP limit. The VDSS originally used the NSSP 3-Tube test (14/49 Standard), and transitioned to the NSSP Membrane Filtration Test (MFT, 14/31 Standard) in August 2007. In this article we focus on a VDSS 13-plus year dataset of fecal coliform concentrations from 127,320 water samples collected from 2,193 sampling stations in 103 shellfish growing areas located in Virginia’s state waters. Our goal is to introduce a new shellfish sanitation model, Mermaid, which provides additional metrics to the NSSP statistical procedures for managing shellfish growing areas under the Direct Rule method, using calculated datasets, with uniform and mixed samples. We also examine if the additional metrics, which are based on the upper limits of Estimated 90th Percentile values of fecal coliform concentrations, increase the health safety of harvested shellfish managed under the Direct Rule method. Keywords: Aquaculture, Computer software, Decision support system, Diagnosis, Fecal coliform, Sanitation model, Shellfish harvesting

1980 ◽  
Vol 43 (2) ◽  
pp. 105-110 ◽  
Author(s):  
R. D. ELLENDER ◽  
J. B. MAPP ◽  
B. L. MIDDLEBROOKS ◽  
D. W. COOK ◽  
E. W. CAKE

The numbers of fecal coliforms and enteroviruses present in oysters and/or their growing waters of two Mississippi reefs were determined over a 12-month period. Bacterial and viral levels reflected the classification of the waters at each location as set by the Mississippi State Board of Health in compliance with the National Shellfish Sanitation Program, but statistically significant correlations between these levels were not observed. Twelve viral isolates were found at an approved oyster harvesting location, eight of which were identified as poliovirus type 1. At the prohibited site, 146 viruses were isolated including poliovirus types 1 and 2, echovirus type 24 and several isolates which remain to be identified. The number of virus isolates from samples from each location represented approximately 35% of the number of plaques observed; however, no consistent ratio of plaque to confirmed virus was demonstrated. The results suggest that the fecal coliform levels in oyster growing waters do not reflect the level of virus contaminaton in either approved or prohibited waters.


Author(s):  
Lise Schoonen ◽  
Marjolein Neele ◽  
Hans van Toor ◽  
Caroline M J van Kinschot ◽  
Charlotte van Noord ◽  
...  

Abstract Context Measurements of thyroglobulin (Tg) and Tg antibodies are crucial in the follow-up of treated differentiated thyroid cancer (DTC) patients. Inter-assay differences may significantly impact follow-up. Objective The aim of this multicenter study was to explore the impact of Tg and Tg antibody assay performance on the differential classification of DTC patients, as described in national and international guidelines. Design Four commonly used Tg and Tg antibody assays were technically compared to reflect possible effects on patients with DTC follow-up. Storage stability at different storage temperatures was also investigated for LIAISON® and Kryptor assays, as this is an underexposed topic in current literature. Results B.R.A.H.M.S. assays yield approximately 50% lower Tg values over the whole range compared to the DiaSorin and Roche assays investigated. These differences between assays may result in potential misclassification in up to 7% of patients if fixed cut-offs (e.g. 1 ng/mL) are applied. Poor correlation was also observed between the Tg antibody assays, when the method-specific upper limits of normal are used as cut-offs. Storage of Tg and Tg antibodies was possible for three to four weeks at -20 °C and -80 °C. Calibration of the assays, however, was found to be crucial for stable results over time. Conclusions Technical aspects of Tg and Tg antibody assays, including inter-assay differences, calibration and standardization, and cut-off values, may have a significant clinical impact on the follow-up of DTC patients.


2014 ◽  
Vol 496-500 ◽  
pp. 1121-1124
Author(s):  
Hong Wen ◽  
Chuan Feng Yao

This paper describes the classification of faults of hydraulic system, diagnosis method. And using the hydraulic system of machine tool to illustrate the common fault of hydraulic system and troubleshooting.


1996 ◽  
Vol 41 (4) ◽  
pp. 108-109
Author(s):  
B.S. Henderson ◽  
M.W. Taylor ◽  
R.M. Milne ◽  
R.J. Taylor ◽  
L.D. Ritchie ◽  
...  

A questionnaire concerning blood pressure assessment, as part of health promotion activity, was circulated to all 770 Gpass practices in Scotland producing a 64.6% response rate. The results reveal a wide range in both the systolic and diastolic levels chosen to classify blood pressure as normal, borderline raised or raised. Practices are using a variety of values to indicate hypertension when considering systolic and, to a lesser extent, diastolic pressure. The variations found suggest that both over and under treatment are a significant risk to patients. The introduction of the 1993 health promotion regulations means that practices are required to actively target their practice population for blood pressure assessment and appropriate intervention. We suggest that this process will be enhanced if doctors are encouraged to adopt the established guidelines for the classification of blood pressure or general practice computer software is adapted to offer blood pressure protocol support.


2018 ◽  
Vol 17 (1) ◽  
pp. 113-123 ◽  
Author(s):  
Meagan R. Jackson ◽  
John Scott Meschke ◽  
Jeremy Simmons ◽  
Tania Busch Isaksen

Abstract Ultraviolet disinfection (UVD) units enhance onsite sewage systems (OSSs) in areas where conventional treatment is limited by site characteristics. Although UVD units are efficacious under testing conditions, few studies have considered their effectiveness when installed. This study used a mixed-methods approach to examine UVD unit effluent quality and determine the association between UV bulb status and fecal coliform levels. Samples from UVD units and pump chambers were tested for bacterial and physiochemical parameters. Field data were supplemented with data from retrospective compliance samples. A multivariate Tobit regression model predicted that the geometric mean (GM) fecal coliform concentration was 122% higher when the UV bulb was deficient than when it was not deficient, adjusted for other OSS deficiencies (95% CI: 36–428, p-value <0.001). The predicted GM fecal coliform concentration in malfunctioning UVD unit effluent (745 CFU/100 mL) exceeded field compliance standards (400 CFU/100 mL), and the odds of exceedance were 7.48 times higher when the UV bulb was deficient, adjusted for other OSS deficiencies (95% CI: 4.03–13.9, p-value <0.001). Despite limitations in the characterization of UV dose, the results validate the importance of UVD units to reduce bacterial loads and the need for further research into their field effectiveness.


1976 ◽  
Vol 22 (12) ◽  
pp. 1774-1776 ◽  
Author(s):  
Susan L. Stramer

Klebsiella pneumoniae is a human and animal pathogen frequently encountered in the environment. The membrane-filtration method utilizing m-FC medium for the detection of fecal coliforms yields a colony type easily distinguishable as K. pneumoniae. These colonies appear as atypical light blue, nucleated, mucoid colonies differing considerably from the typical dark blue fecal coliform colonies.


2018 ◽  
Vol 17 (2) ◽  
pp. 25-31 ◽  
Author(s):  
Bikram Gautam ◽  
Rameshwar Adhikari

Introduction: Membrane filtration (MF) is one of the widely used technique on a routine basis. On the other hand, replica plate (RP) technique can be used to transfer existing bacterial colonies in two plates which even allows pinpointing the original colony. The aim of this study is to comparatively detect the cfu/100 mL of fecal coliform using membrane filtration and replica plate techniques.Methods: In the study, a total of 25 bottled water were selected from the local market in Kathmandu valley. The total coliform count was detected using MF, while fecal coliform was detected using both MF and RP technique.Results: It was found that the average cfu/100 mL for total coliform, fecal coliform (MF) and fecal coliform (RP) were 143.38, 49.82 and 51.00 respectively. Pearson correlation coefficient calculated between total coliform and fecal coliform (MF), total coliform and fecal coliform (RP), fecal coliform (MF) and fecal coliform (RP) were found to be 0.695, 0.733 and 0.990 respectively; implying a positive correlation Conclusions: It has been demonstrated that intrinsic and extrinsic factors influence colony forming units. Furthermore, RP is a more sensitive method for screening fecal coliforms although both MF and RP can be efficiently used.


1984 ◽  
Vol 106 (2) ◽  
pp. 441-447 ◽  
Author(s):  
W. W. Yuen

The calculation of the geometric-mean transmittance factor between areas with an intervening absorbing and isotropically scattering medium is considered. While an exact expression for the factor is shown to be quite complicated, the upper and lower limits of the factor can be readily generated from physical consideration. Integral expressions for successively increasing (decreasing) values of the lower (upper) limits are obtained. For two-dimensional systems, these expressions are reduced to integrals involving Sn (x), a class of exponential integral function that has been tabulated in a previous work. Utilizing the kernel substitution technique, these integrals are evaluated analytically in closed form for some selected geometries. For cases with small optical thickness and large scattering albedo, both limits are shown to converge relatively slowly to the actual transmittance factor. But the decreasing difference between the two limits provides accurate estimate of the geometric-mean transmittance factor. Based on these results, some interesting conclusions concerning the effect of scattering on multidimensional radiative transmission are established.


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