scholarly journals Impact of thyroglobulin and thyroglobulin antibody assay performance on the differential classification of DTC patients

2021 ◽  
Author(s):  
Lise Schoonen ◽  
Marjolein Neele ◽  
Hans van Toor ◽  
Caroline M J van Kinschot ◽  
Charlotte van Noord ◽  
...  
Keyword(s):  
Poor Correlation ◽  
Antibody Assay ◽  
Thyroglobulin Antibody ◽  
Assay Performance ◽  
Upper Limits ◽  
Antibody Assays ◽  
The Impact ◽  
Storage Temperatures

Abstract Context Measurements of thyroglobulin (Tg) and Tg antibodies are crucial in the follow-up of treated differentiated thyroid cancer (DTC) patients. Inter-assay differences may significantly impact follow-up. Objective The aim of this multicenter study was to explore the impact of Tg and Tg antibody assay performance on the differential classification of DTC patients, as described in national and international guidelines. Design Four commonly used Tg and Tg antibody assays were technically compared to reflect possible effects on patients with DTC follow-up. Storage stability at different storage temperatures was also investigated for LIAISON® and Kryptor assays, as this is an underexposed topic in current literature. Results B.R.A.H.M.S. assays yield approximately 50% lower Tg values over the whole range compared to the DiaSorin and Roche assays investigated. These differences between assays may result in potential misclassification in up to 7% of patients if fixed cut-offs (e.g. 1 ng/mL) are applied. Poor correlation was also observed between the Tg antibody assays, when the method-specific upper limits of normal are used as cut-offs. Storage of Tg and Tg antibodies was possible for three to four weeks at -20 °C and -80 °C. Calibration of the assays, however, was found to be crucial for stable results over time. Conclusions Technical aspects of Tg and Tg antibody assays, including inter-assay differences, calibration and standardization, and cut-off values, may have a significant clinical impact on the follow-up of DTC patients.

scholarly journals SARS-CoV-2 anti-nucleocapsid assay performance in healthcare workers at baseline and 6 months

2021 ◽  
Author(s):  
Colm Kerr ◽  
Niamh Allen ◽  
Gerry Hughes ◽  
Martina Kelly ◽  
Fiona O’Rourke ◽  
...  
Keyword(s):  
Healthcare Workers ◽  
Threshold Values ◽  
Antibody Assay ◽  
Antibody Reactivity ◽  
Assay Performance ◽  
Significant Difference ◽  
History Of ◽  
Pandemic Response ◽  
Roche Diagnostics

Abstract Introduction Serological SARS-CoV-2 assays have an important role in guiding the pandemic response. This research aimed to compare the performance of 2 antinucleocapsid assays. Methods Serum from 49 HCWs was analysed at baseline and 6 months using the Abbott diagnostics SARS-CoV-2 IgG assay and the Roche Diagnostics Elecsys Anti-SARS-CoV-2 total antibody assay. Results At baseline, 14/49 participants (29%) demonstrated antibody reactivity using the Abbott assay. At 6 months, 4/14 participants (29%) continued to demonstrate reactivity. A total of 14/49 (29%) participants had detectable antibodies at baseline using the Roche assay. In total, 13/14 (93%) of participants demonstrated antibody reactivity at 6 months. The Abbott assay showed a statistically significant difference in the signal-to-threshold values of baseline reactive samples when repeated at 6 months (p = 0.001). This was not seen with the Roche assay (p = 0.51). Conclusion In this small study, the Roche Diagnostics Elecsys Anti-SARS-CoV-2 total antibody assay appears superior in performance to the Abbott diagnostics SARS-CoV-2 IgG assay in accurately detecting participants with a history of confirmed COVID-19 disease at 6 months follow-up. This finding should be born in mind in the planning of future seroprevalence studies, especially when considering the use of anti-nucleocapsid assays.

scholarly journals COVID-19 pandemic: impact of lockdown and hospital classification on neurological patients

2020 ◽  
Vol 7 (8) ◽  
pp. 2985
Author(s):  
Amandeep Singh ◽  
Nitisha Goyal ◽  
Dinesh Chouksey ◽  
Kapil Telang ◽  
Rahul Jain ◽  
...  
Keyword(s):  
Human Life ◽  
Symptom Control ◽  
P Value ◽  
Study Group ◽  
Drug Side Effects ◽  
Monitoring Drug ◽  
Neurological Patients ◽  
The Impact

Background: COVID-19 pandemic has impacted human life by the steps taken to mitigate it. The restriction of movement and earmarking hospitals exclusively for catering to the COVID-19 patients might have affected the health of non-COVID-19 patients adversely. This study was aimed to assess the impact of lockdown and hospital classification in neurological patients.Methods: This study was conducted in the Department of Neurology in a 1000 bedded teaching hospital which has been earmarked as COVID dedicated hospital (the red hospital). The patients who attended the outpatient department and discharged from the Neurology department, prior to the lockdown, were contacted telephonically and asked to report their difficulties, if any, in the procurement of prescribed medication, medical consultation, monitoring drug side effects, and symptom control, etc. Patients were grouped into those who needed close surveillance and routine follow up.Results: Out of 1201 patients 646 could be reached. The average age of our population (n=646, female 56.4%) was 39.63±15.52 years. In the study group, the prescription breach was found in 343 patients (53.10%), management breach was in 449 (69.50%), confidence breach was in 398 (61.61%) and adverse effect on drug availability was found in 330 (53.10%) patients. In the close surveillance group (n=420), the patient's health was deemed to be at risk in 328 (78.10%) patients as compared to 161 patients (71.24%) in routine follow up group with a significant p value of 0.05.Conclusions: Our results suggest that lockdown and classification of hospitals to cater exclusively to COVID-19 patients has significantly ill-affected the health of the non-COVID subjects.

Comparison of protein-based cell-of-origin classification to the Lymph2Cx RNA assay in a cohort of diffuse large B-cell lymphomas in Malaysia

2017 ◽  
Vol 71 (3) ◽  
pp. 215-220 ◽  
Author(s):  
Kean-Chang Phang ◽  
Ariz Akhter ◽  
Nur Maya Sabrina Tizen ◽  
Faridah Abd Rahman ◽  
Raja Zahratul Azma ◽  
...  
Keyword(s):  
B Cell ◽  
B Cell Lymphoma ◽  
Rank Test ◽  
Cell Of Origin ◽  
Log Rank Test ◽  
Assay Performance ◽  
The Impact ◽  
Digital Quantification ◽  
Large B Cell

AimsThe cell of origin (COO) based molecular characterisation into germinal centre B-cell-like (GCB) and activated B-cell-like (ABC) subtypes are central to the pathogenesis and clinical course in diffuse large B-cell lymphoma (DLBCL). Globally, clinical laboratories employ pragmatic but less than ideal immunohistochemical (IHC) assay for COO classification. Novel RNA-based platforms using routine pathology samples are emerging as new gold standard and offer unique opportunities for assay standardisation for laboratories across the world. We evaluated our IHC protocols against RNA-based technologies to determine concordance; additionally, we gauged the impact of preanalytical variation on the performance of Lymph2Cx assay.MethodsDiagnostic biopsies (n=104) were examined for COO classification, employing automated RNA digital quantification assay (Lymph2Cx). Results were equated against IHC-based COO categorisation. Assay performance was assessed through its impact on overall survival (OS).Results96 (92%) informative samples were labelled as GCB (38/96; 40%) and non-GCB (58/96; 60%) by IHC evaluation. Lymph2Cx catalogued 36/96 (37%) samples as GCB, 45/96 (47%) as ABC and 15/96 (16%) as unclassified. Lymph2Cx being reference, IHC protocol revealed sensitivity of 81% for ABC and 75% for GCB categorisation and positive predictive value of 81% versus 82%, respectively. Lymph2Cx-based COO classification performed superior to Hans algorithm in predicting OS (log rank test, p=0.017 vs p=0.212).ConclusionsOur report show that current IHC-based protocols for COO classification of DLBCL at UKM Malaysia are in line with previously reported results and marked variation in preanalytical factors do not critically impact Lymph2Cx assay quality.

Estimation of Elastic Follow-Up in Structures

2011 ◽  
Vol 462-463 ◽  
pp. 361-365 ◽  
Author(s):  
Saeid Hadidi-Moud ◽  
David John Smith
Keyword(s):  
Residual Stresses ◽  
Closed Form ◽  
Analytical Solutions ◽  
Unified Framework ◽  
Integrity Assessment ◽  
The Impact ◽  
Model Structures

Using simplified benchmark models, representative of the behavior of real structures, a unified framework for quantification of elastic follow-up (EFU) in structures has been provided. Closed form analytical solutions for evaluation of elastic follow-up are presented for model structures. The impact of elastic follow-up on the relaxation i.e. the redistribution of residual stresses has been explored and hence its significance in the integrity assessment of structures in general and in classification of residual stresses in particular has been highlighted.

scholarly journals The impact of stellar jitter on the confirmation of transiting exoplanet candidates around Solar-like stars

2008 ◽  
Vol 4 (S253) ◽  
pp. 506-507
Author(s):  
Brandon Tingley ◽  
Frank Grundahl ◽  
Hans Kjeldsen
Keyword(s):  
Time Series ◽  
Monte Carlo ◽  
Monte Carlo Simulations ◽  
Signal Detection ◽  
Radial Velocity ◽  
Good Description ◽  
Sampling Rate ◽  
Upper Limits ◽  
The Impact

AbstractThe radial velocity technique is commonly used to classify transiting exoplanet candidates. However, stars are intrinsically noisy in radial velocity. No good description of this noise has yet been proffered, although activity in general has been suggested as the source, making it impossible to evaluate its effect on signal detection. In this poster, we propose an activity-based model that incorporates both light and dark stellar spots, capable of producing both photometric and radial velocity time series. We demonstrate its consistency with both SOHO/VIRGO photometry and SOHO/GOLF radial velocities. We then use this model to establish lower and upper limits on the effects of intrinsic stellar noise on the metal lines used to follow up transit candidates, making use of Monte Carlo simulations. Based on these results, we can suggest an optimal observational sampling rate.

scholarly journals P061 ANALYSIS OF THE IMPACT DERIVED FROM MORBIDITY IN THE INCISIONAL HERNIA SURGERY

2021 ◽  
Vol 108 (Supplement_8) ◽  
Author(s):  
Carles Olona ◽  
Alba Varona Mancilla ◽  
Jordi Vadillo Bargalló ◽  
Marc Vallvé Bernal ◽  
Joan Ferreres Serafini ◽  
...  
Keyword(s):  
Incisional Hernia ◽  
Hernia Surgery ◽  
Anthropometric Parameters ◽  
European Hernia Society ◽  
The Impact ◽  
Level Hospital ◽  
Final Evolution ◽  
Prospective Database

Abstract Aim To analyse the repercussion derived from the morbidity that appeared after the repair of incisional hernias. Material and Methods Descriptive, retrospective and longitudinal study based on a prospective database of all incisional hernia operated patients of a university-level hospital in the period between 2013 and 2019. Anthropometric parameters, classification of the hernia according to the European Hernia Society guides and characteristics of the intervention performed were registered. Complications observed until the end of the follow-up and the final evolution are analysed. Results The sample is composed by 244 patients with a mean follow-up of 11 months. In 50% of the surgeries anterior separation of components was performed. In 240 patients, the mesh was placed in the following positions: 68.5% onlay, 13.5% retromuscular, 13% preperitoneal, 3.6% intraperitoneal and 1.3% inlay. During the first postoperative month, 63 patients (25.8%) presented some type of complication: seroma (47.6%), surgical wound infection (20.6%) and dehiscence (17.5%). 31 complications (49.2%) were classified as Clavien-Dindo III or greater severity. In 60% of patients with seroma the mesh was placed onlay. Of the 30 patients with seroma, 9 recurred over a mean of 16 months of follow-up (30%). Conclusions In our sample, the main cause of postoperative seroma is onlay placement of the mesh, and this complication may cause recurrence. We consider that it’s important to register our results to obtain a correct analysis and to prevent morbidity in the future.

Genetic diversity of human immunodeficiency viruses and antiretroviral therapy

2016 ◽  
Vol 88 (11) ◽  
pp. 103-111 ◽  
Author(s):  
M R Bobkova
Keyword(s):  
Genetic Diversity ◽  
Antiretroviral Therapy ◽  
Therapeutic Effects ◽  
Resistance Mutations ◽  
Origin And Evolution ◽  
Treatment Regimens ◽  
Human Immunodeficiency Viruses ◽  
The Impact

The lecture is devoted to the analysis of the state-of-the-art of the impact of genetic diversity of human immunodeficiency (HIV) viruses on the pattern of infection and the efficiency of antiretroviral therapy (ART). It provides brief information on the origin and evolution of HIV and on the current classification of their genetic variants. The molecular epidemiological situation of HIV infection in Russia and nearby states and the major molecular HIV variants that are dominant in these countries, as well as their origin and prevalence trends are characterized. How the diversity of HIV can affect the efficiency of diagnosis, the transmission of the virus, and the pattern of HIV pathogenesis are briefly reviewed. The comparative data available in the world’s scientific literature on these topics are given. More detailed attention is given to the possible causes of varying therapeutic effects against different HIV subtypes, as well as to the specific features of the formation and phenotyping manifestation of ART drug resistance mutations. There is evidence for the necessity of forming a unified follow-up system for treated HIV-infected patients during ART scaling, including in an effort to evaluate the impact of the specific features of the HIV genome on the efficiency of treatment regimens used in Russia.

Evaluation and Follow-Up of Carotid-Cavernous Fistulas by Transcranial Doppler Sonography: Illustrative Case

Neurosurgery ◽  
1989 ◽  
Vol 24 (5) ◽  
pp. 749-753 ◽  
Author(s):  
Camilo R. Gomez ◽  
Sandra M. Gomez ◽  
Kong-Woo Peter Yoon ◽  
Gary E. Kraus
Keyword(s):  
Transcranial Doppler ◽  
Doppler Sonography ◽  
Transcranial Doppler Sonography ◽  
Flow Characteristics ◽  
Illustrative Case ◽  
Computed Tomographic ◽  
Carotid Cavernous Fistulas ◽  
The Impact

ABSTRACT The availability of transcranial Doppler sonography has resulted in an easy, noninvasive, reproducible, and highly reliable method for evaluating the flow characteristics of carotid-cavernous sinus fistulas. It also allows the follow-up of the effect of different interventional measures, specifically, embolization with detachable balloons. An illustrative case is reported, in which the findings of serial transcranial Doppler sonograms are correlated with findings on computed tomographic scans and angiograms. The impact of our findings on future approaches to the hemodynamic classification of these acquired vascular shunts is discussed.

P1002Echocardiographic assessment of optimal device position after percutaneous left atrial appendage occlusion - introduction of a novel classification and its impact on outcome

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
V O Vij ◽  
B Al-Kassou ◽  
D Nelles ◽  
M Stuhr ◽  
R Schueler ◽  
...  
Keyword(s):  
Left Atrial ◽  
Left Atrial Appendage ◽  
Transoesophageal Echocardiography ◽  
Atrial Appendage ◽  
The Novel ◽  
Left Atrial Appendage Occlusion ◽  
Implantation Depth ◽  
The Impact

Abstract Background Left atrial appendage occlusion (LAAo) is an established therapy in patients with atrial fibrillation. However, criteria regarding optimal device position are not well defined making comparability of procedural results virtually impossible. We therefore sought to a) introduce a classification describing optimal vs. suboptimal device-position by assessing predefined parameters in transoesophageal echocardiography (TEE) and to b) analyze the impact of device-position on outcome in patients treated with different LAAo devices. Methods and results We retrospectively analyzed 120 patients who were treated by LAAo and had undergone follow-up TEEs after 3 or 6 months. Patients were at mean age: 76±8 years; female 40% and presented an increased CHADS-VASC- (4.6±1.4) and HAS-BLED-score (3.7±1). TEE-guidance was performed in all cases. In 62.5% (75/120) pacifier occluders (PO) (ACP/Amulet, Lambre, Ultraseal) were used, whereas 37.5% (45/120) were treated with non-pacifier occluders (NPO) (Watchman, Wavecrest, Occlutech). To assess device position, TEE images in a commissural view (60–90°) were analyzed and characterised by 1) implantation depth in the left atrial appendage, 2) peridevice flow (PF) and 3) the angle between occluder disc and pulmonal ridge (LUPV). For the purpose of this study, optimal device position was defined as a) ostial (LUPV length <10mm) or slightly subostial position (LUPV length ≤15mm, angle ≥100°) with b) the absence of major PF (>3mm). Overall, occluders were implanted at a depth of 12±7.8 mm with ostial positioning being achieved in 47.5% (57/120). Major PF was seen in 7.5% (9/120). NPOs were implanted deeper than POs (depth: 15.6±7.1 vs. 9.8±7.4 mm, p<0.01; ostial position: 31.1% vs. 57.3%, p<0.01) and were associated with a higher incidence of major PF (15.6% vs. 2.7%, p=0.01). Also, the depth/angle ratio was higher (i.e. “worse”) in NPOs (18.3±9 vs. 14.6±8, p<0.04). As a result, optimal device position was achieved in 48.3% (58/120) of all patients, with lower rates in NPOs than in POs (26.7% vs. 61.3%, p<0.01). Procedural aspects revealed slight differences in occluder size (optimal: 23.7±3.2 vs. suboptimal: 24.5±3.7 mm, p=0.3), need for repositioning (10.3% vs. 17.7%, p=0.25) and procedural duration (48±36 vs. 52±34 min, p=0.3). Of interest, device related thrombi (DRT) occurred less frequently in optimally implanted devices (3.4% vs. 12.9%, p=0.06). Hereby, implantation depth and depth/angle ratio were found to be predictors for DRT in ROC-analysis, respectively (AUC: 0.7, 95% Confidence interval [CI]: 0.56–0.84, p=0.05 and AUC: 0.72, 95% CI: 0.58–0.86, p=0.03). Optimal vs. suboptimal position Conclusion Echocardiographic classification of device-position is warranted to provide comparability and appears to be feasible. Based on the novel classification provided, optimal device-position is achieved in 50% and is found more often with the use of POs. DRT appeared to occur more often in suboptimal device-position.

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