The phenotypic appearance of Japanese persimmon (Diospyros kaki L.f.) in Karo District, North Sumatra, Indonesia

Hanafiah DS, Sanggita S, Lubis K. 2018. The phenotypic appearance of Japanese persimmon (Diospyros kaki L.f.) in Karo District, North Sumatra, Indonesia. Biodiversitas 19: 509-514. The farmers in Karo District began to be less interested in cultivating kesemek or Japanese persimmon (Diospyros kaki L.f.). Due to the price of this commodity has dropped in the market, the farmers cut down the tree and cultivate more favorable horticultural crops, such as vegetable and citrus cultivation. It is necessary to conduct a study to obtained sufficient information to determine the necessary steps in order to preserve Japanese persimmon plants in Karo District. One first steps was to get information of accession characteristic of Japanese persimmon in Karo District. This research aimed to identify relationships of morphological characteristics among individuals of Japanese persimmon plants. The research was conducted from May until July 2017 at Berastagi, Merdeka, Kabanjahe, and Dolat Rayat sub-districts of Karo District, North Sumatra, Indonesia. The survey method using Japanese persimmon descriptor of Diospyros kaki L.f. by the International Union for the Protection of New Varieties (UPOV) with the purposive method for location determination and technique sampling. Thirty-six accessions of Japanese persimmon plants were studied. Based on clustering method of the morphological characteristic, 4 clusters are formed. This difference was canopy form, leaf shape and fruit morphological. Accession 34 from Dolat Rayat Distict has different fruit characters with other accessions.

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Abnormalities of Seed Development in a Parthenocarpic Japanese Persimmon, Diospyros kaki cv. Hiratanenashi

Engei Gakkai zasshi ◽

10.2503/jjshs.59.65 ◽

1990 ◽

Vol 59 (1) ◽

pp. 65-73 ◽

Cited By ~ 4

Author(s):

Masashi ISHIDA ◽

Masami KONISHI ◽

Akira KITAJIMA ◽

Yoshitsugu SOBAJIMA

Keyword(s):

Seed Development ◽

Diospyros Kaki ◽

Japanese Persimmon

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First Report of Flyspeck Caused by Zygophiala wisconsinensis on Sweet Persimmon Fruit in Korea

Plant Disease ◽

10.1094/pdis-12-10-0917 ◽

2011 ◽

Vol 95 (5) ◽

pp. 616-616 ◽

Cited By ~ 1

Author(s):

J. Kim ◽

O. Choi ◽

J.-H. Kwon

Keyword(s):

Disease Incidence ◽

Morphological Characteristics ◽

Its Rdna ◽

Control Treatment ◽

Diospyros Kaki ◽

Distilled Water ◽

First Report ◽

Persimmon Fruit ◽

Fungal Isolates ◽

Agar Disc

Sweet persimmon (Diospyros kaki L.), a fruit tree in the Ebenaceae, is cultivated widely in Korea and Japan, the leading producers worldwide (2). Sweet persimmon fruit with flyspeck symptoms were collected from orchards in the Jinju area of Korea in November 2010. The fruit had fungal clusters of black, round to ovoid, sclerotium-like fungal bodies with no visible evidence of a mycelial mat. Orchard inspections revealed that disease incidence ranged from 10 to 20% in the surveyed area (approximately 10 ha) in 2010. Flyspeck symptoms were observed on immature and mature fruit. Sweet persimmon fruit peels with flyspeck symptoms were removed, dried, and individual speck lesions transferred to potato dextrose agar (PDA) and cultured at 22°C in the dark. Fungal isolates were obtained from flyspeck colonies on 10 sweet persimmon fruit harvested from each of three orchards. Fungal isolates that grew from the lesions were identified based on a previous description (1). To confirm identity of the causal fungus, the complete internal transcribed spacer (ITS) rDNA sequence of a representative isolate was amplified and sequenced using primers ITS1 and ITS4 (4). The resulting 552-bp sequence was deposited in GenBank (Accession No. HQ698923). Comparison with ITS rDNA sequences showed 100% similarity with a sequence of Zygophiala wisconsinensis Batzer & Crous (GenBank Accession No. AY598855), which infects apple. To fulfill Koch's postulates, mature, intact sweet persimmon fruit were surface sterilized with 70% ethanol and dried. Three fungal isolates from this study were grown on PDA for 1 month. A colonized agar disc (5 mm in diameter) of each isolate was cut from the advancing margin of a colony with a sterilized cork borer, transferred to a 1.5-ml Eppendorf tube, and ground into a suspension of mycelial fragments and conidia in a blender with 1 ml of sterile, distilled water. The inoculum of each isolate was applied by swabbing a sweet persimmon fruit with the suspension. Three sweet persimmon fruit were inoculated per isolate. Three fruit were inoculated similarly with sterile, distilled water as the control treatment. After 1 month of incubation in a moist chamber at 22°C, the same fungal fruiting symptoms were reproduced as observed in the orchards, and the fungus was reisolated from these symptoms, but not from the control fruit, which were asymptomatic. On the basis of morphological characteristics of the fungal colonies, ITS sequence, and pathogenicity to persimmon fruit, the fungus was identified as Z. wisconsinensis (1). Flyspeck is readily isolated from sweet persimmon fruit in Korea and other sweet persimmon growing regions (3). The exposure of fruit to unusual weather conditions in Korea in recent years, including drought, and low-temperature and low-light situations in late spring, which are favorable for flyspeck, might be associated with an increase in occurrence of flyspeck on sweet persimmon fruit in Korea. To our knowledge, this is the first report of Z. wisconsinensis causing flyspeck on sweet persimmon in Korea. References: (1) J. C. Batzer et al. Mycologia 100:246, 2008. (2) FAOSTAT Database. Retrieved from http://faostat.fao.org/ , 2008. (3) H. Nasu and H. Kunoh. Plant Dis. 71:361, 1987. (4) T. J. White et al. Page 315 in: PCR Protocols: A Guide to Methods and Applications. M. A. Innis et al., eds. Academic Press, Inc., New York, 1990.

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Characterization and comparison of EST-SSR and TRAP markers for genetic analysis of the Japanese persimmon Diospyros kaki

Genetics and Molecular Research ◽

10.4238/2013.january.9.3 ◽

2013 ◽

Vol 12 (AOP) ◽

Cited By ~ 2

Author(s):

C. Luo ◽

F. Zhang ◽

Q.L. Zhang ◽

D.Y. Guo ◽

Z.R. Luo

Keyword(s):

Genetic Analysis ◽

Diospyros Kaki ◽

Japanese Persimmon

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First Report of Anthracnose Caused by Colletotrichum acutatum on Persimmon Fruit in the United States

Plant Disease ◽

10.1094/pdis-94-5-0634a ◽

2010 ◽

Vol 94 (5) ◽

pp. 634-634 ◽

Cited By ~ 7

Author(s):

S. M. Williamson ◽

T. B. Sutton

Keyword(s):

United States ◽

Filter Paper ◽

The United States ◽

Grocery Store ◽

Fruit Rot ◽

Colletotrichum Acutatum ◽

Diospyros Kaki ◽

Japanese Persimmon ◽

First Report ◽

Persimmon Fruit

Persimmon trees are important for their fruit as well as their colorful fruit and foliage in the fall. Persimmon fruit (Japanese persimmon, Diospyros kaki cv. Fuyu) were collected in November 2008 from a tree in Windsor, NC, located in the Coastal Plain. Fruit were not symptomatic on the tree but developed dark lesions after harvest. Isolations from six fruit yielded seven isolates of Colletotrichum acutatum J. H. Simmonds. After incubation at 25°C under continuous light for 15 days on potato dextrose agar (PDA), all isolates had gray aerial mycelium, but the inverse sides of the plates of six isolates were maroon and one was beige. Masses of salmon-colored conidia were formed first in the center of the colonies, then were observed scattered across the colonies in older cultures. Conidia were hyaline, one-celled, elliptic with one or both ends pointed, and measured 8.1 to 16.3 × 3.1 to 5 μm. Setae and sclerotia were not observed. There were also dark structures measuring 1 to 10 mm that were partially embedded in the agar that contained conidia. Cultural and conidial characteristics of the isolates were similar to those of C. acutatum (3). PCR amplification was performed with the species-specific primer pair CaInt2/ITS4 (2) and genomic DNA from the original isolates and isolates obtained from inoculated fruit. An amplification product of approximately 490 bp, which is specific for C. acutatum, was observed. To fulfill Koch's postulates, persimmon fruit obtained from the grocery store were surface disinfested with 0.5% sodium hypochlorite and sterile filter paper disks dipped in conidial suspensions (1 × 105 conidia/ml) of two C. acutatum isolates (maroon and beige reverse) or sterile, deionized water were placed on the fruit. Three fruit were inoculated per treatment and the disks were placed on four locations on each fruit. Parafilm was wrapped around the diameter of the fruit to keep the filter paper disks moist and in place. Fruit were placed in moist chambers and incubated at 25°C. After 3 days, the Parafilm was removed and the fruit returned to the moist chambers. Small, dark lesions were observed on fruit inoculated with each isolate of C. acutatum when the filter paper disks were removed. Ten days after inoculation, dark lesions and acervuli with salmon-colored masses of conidia were observed on fruit inoculated with both isolates of C. acutatum and the fruit were soft. After 12 days, there were abundant masses of conidia and the inoculated areas were decayed. Control fruit remained firm and did not develop symptoms. Cultures obtained from the fruit and the conidia produced were typical of the isolates used to inoculate the fruit. C. acutatum has been reported to cause fruit rot on persimmon fruit in New Zealand (1). To our knowledge, this is the first report of C. acutatum on persimmon fruit in the United States. References: (1) R. Lardner et al. Mycol. Res. 103:275, 1999. (2) S. Sreenivasaprasad et al. Plant Pathol. 45:650, 1996. (3) B. C. Sutton. Page 523 in: Coelomycetes. Commonwealth Agricultural Bureaux, Great Britain. 1980.

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Do we have infraspecific taxa of Salvia multicaulis Vahl. (Lamiaceae) in Iran?

Ukrainian Journal of Ecology ◽

10.15421/2017_138 ◽

2017 ◽

Vol 7 (4) ◽

pp. 432-439 ◽

Cited By ~ 1

Author(s):

Seyed Mehdi Talebi ◽

Reza Rezakhanlou ◽

A V. Matsyura

Keyword(s):

Leaf Shape ◽

Morphological Characteristics ◽

Leaf Length ◽

Width Ratio ◽

Petiole Length ◽

Widespread Species ◽

Basal Leaf ◽

Leaf Petiole ◽

Upgma Cluster Analysis ◽

Length Width

Salvia multicaulis is a widespread species of Lamiaceae family in Iran. There are many discussions about its infraspecific variations. Although some varieties were definite for this species in various parts of the world, no infraspecific taxon was reported in Iran and all samples of this species were named as S. multicaulis. In this study, morphological characteristics of S. multicaulis populations, naturally growing in Iran, was examined. Twenty-two traits were examined in 94 individuals of this species to identify their phenotypic difference. Most of the investigated features were showing a high degree of variability, but it was highly pronounced for some characteristics such as basal leaf shape, basal leaf width, basal leaf length/ width ratio and basal petiole length. Significant positive/negative correlations were observed between some morphological variables. Furthermore, significant negative correlations were found between the eastern distribution of populations with basal leaf petiole length and basal leaf length/ width ratio. Based on the UPGMA cluster analysis, populations were divided into two main branches. The first branch contained four populations, while the second branch was bigger and clustered in two sub-branches. In one of them, three populations and in another one the rest populations arranged in two groups. CA joined plot confirmed that each of studied populations or group of populations had distinct morphological trait(s), which were useful in identification of them. Our findings supported population no. 13 had unique morphological traits such as the largest bracts and basal leaf petiole, highest flower number of each inflorescence cycle, widest and largest calyx. The conservation of the highly diverse populations of Iranian S. multicaulis is recommended.

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To the question of the origin of the cultural assortment of Dagestan apricots through assessing the variability of leaf morphological characteristics

South of Russia ecology development ◽

10.18470/1992-1098-2019-3-17-24 ◽

2019 ◽

Vol 14 (3) ◽

pp. 17-24

Author(s):

D. M. Anatov ◽

Z. M. Аsadulaev ◽

R. M. Osmanov ◽

K. I. Akhmedova

Keyword(s):

Leaf Shape ◽

Morphological Characteristics ◽

Principal Component ◽

Leaf Size ◽

Shape Index ◽

Botanical Garden ◽

Mahalanobis Distances ◽

Traditional Cultivars ◽

Academy Of Sciences ◽

Degree Of Similarity

Aim. The paper presents the results of assessment of the indigenous nature and degree of similarity of apricot cultivars growing in the collection of the Mountain Botanical Garden, Gunib, Dagestan, Russia based on a comparative analysis of the variability of leaf morphological characteristics. Material and Methods. The material assessed consisted of 33 apricot cultivars of various ecological and geographical origins aggregated in the following groups: (a) Dagestan – traditional cultivars; (b) Moscow ‐ selection from the Tsytsin Main Moscow Botanical Garden, Russian Academy of Sciences based on wild forms of Tajikistan and Kyrgyzstan; (c) European and (d) Asian ‐ from Central Asia, Tajikistan, China and Altai. Results. The closeness of Dagestan and European varieties in comparison with Asian and Moscow varieties was shown. Most Dagestan (16 of 19) and European varieties have round‐shaped leaves (leaf shape index 80‐ 100%), while those from Asia and the Moscow Botanical Garden have leaves which are elongated elliptical and oval (60‐80%). Using the method of principal component analysis (PCA), it was established that most cultivars of Dagestan origin have similar leaf shapes and sizes, of which Tlama kurak (wide‐round), Hekobarsh (elongated) were distinguished by leaf shape and Esdelik by leaf size. Conclusion. Based on a discriminant analysis (Squared Mahalanobis Distances), it was found that the indices of indicators of leaf attributes (width/length of leaf lamina; petiole length/length of lamina; apex angle/corner of leaf base) are more reliable criteria for differentiating apricot varieties into ecological and geographical groups than their morphological characteristics.

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