scholarly journals Genetic diversity of Amorphophallus titanum in Bengkulu, Indonesia based on RAPD markers

2018 ◽  
Vol 19 (5) ◽  
pp. 1783-1790
Author(s):  
WAHYUDI ARIANTO ◽  
ERVIZAL A.M. ZUHUD ◽  
AGUS HIKMAT ◽  
TUTUT SUNARMINTO ◽  
ISKANDAR Z. SIREGAR
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Rapd Marker ◽  
Natural Habitat ◽  
Land Uses ◽  
Forest Land ◽  
Protected Forests ◽  
The Family ◽  
Center Of Diversity ◽  
Two Populations

Arianto W, Zuhud EAM, Hikmat A, Sunarminto T, Siregar IZ. 2018. Genetic diversity of Amorphophallus titanum in Bengkulu, Indonesia based on RAPD markers. Biodiversitas 19: 1783-1790. Titan Arum [Amorphophallus titanum (Becc.) Becc. Ex Arcang], a plant species belonging to the family of Araceae is known for its gigantic floral size and elicited rotten fragrance when the flower bloom. Since it remains only found in Sumatran island, many authors categorized the plant as endemic species. The population of the species in the natural habitat has significantly declined because of the conversion of forest land mainly into plantations or other land uses. Considering the importance of conservation attempts to A. titanum, a sufficient data on genetic diversity of the species is necessary. The research was aimed to determine the genetic diversity within and among populations of A. titanum in some area of protected forests in Bengkulu Province, comprising the population of Palak Siring, Tebat Monok, and Air Selimang. RAPD genetic DNA fingerprinting approach was used to assess the genetic diversity of A. titanum using 13 preselected DNA primer: OPA 11, OPA 19, OPC 04, OPN 14, OPN 19, OPU 03, OPU 06, OPU 07, OPB 17, OPC 07, OPO 04, OPU03-1, OPNI 18E. The result revealed that the method has successfully produced several DNA fragments with varied length ranging from 250 bp to 2000 bp with 4-16 variation in polymorphic bands. Based on RAPD marker analysis, the population of Air Selimang was considered as a potential center of diversity of A. titanum because of the others two populations had a lower genetic diversity. In general, the genetic diversity among populations was lower than within population. The cluster analysis of the genetic similarity of 22 individuals of the three populations resulted in the separation into two main groups with the first group consisting of 17 individuals (Population Air Selimang and Tebat Monok) and the second group of 5 individuals (Palak Siring population).

461 PB 292 MAPPING RAPD MARKER DIVERSITY IN PHASEOLUS VULGARIS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 497c-497
Author(s):  
Paul Skroth ◽  
Jim Nienhuis ◽  
Geunhwa Jung ◽  
Dermont Coyne
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Genetic Relationships ◽  
Rapd Marker ◽  
Plant Genetic Resources ◽  
Ex Situ ◽  
Good Tool ◽  
Germplasm Collections ◽  
Efficient Construction ◽  
Two Populations

Knowledge of genetic relationships and genetic diversity among accessions is essential for the efficient construction, maintainance and utilization of large ex-situ germplasm collections. Furthermore, streamlining of large collections into care collections necessitates validation of germplasm sampling techniques. DNA molecular markers provide potentially unbiased estimators of genome diversity end may facilitate organization, maintainance, and sampling of plant genetic resources. Our data suggests that RAPD markers will be o good tool for testing tore collection concepts and organizing genetic diversity in common bean. However, the genomic distribution of markers is unknown. Currently we are using recombinant inbred (RI) populations to place RAPD markers in the context of the bean genetic map. We hove evaluated the the distribution of RAPD markers in three RI populations: Bat93 × Jalo EEP558, PC50 × Xan159, and BAC6 × HT7719. Cultivated P.vulgaris has two primary renters of diversity Mesoamerican and Andean, the RI populations used for mapping RAPD markers ore Meso × Andean, Andean × Andean, and Meso × Meso crosses respectively. In the Bat93 × Jalo EEP558 population 383 markers have been mapped for a map length of 735 cM. However, approximately 150 of these markers ore members of 9 dusters which span only 90 cM. This inter gone pool mop is being integrated with linkage mops constructed in the other two populations to compare within and between gene pool marker distributions and to evaluate clustering of markers on the different mops. Implications for the application of RAPD markers will be discussed.

Genetic Diversity among Calamus brandisii Becc. (Arecaceae) Populations in Kerala Part of Western Ghats

2021 ◽  
Vol 43 (1) ◽  
pp. 38-42
Author(s):  
Kavungal Priya ◽  
◽  
Indira . ◽  
Vadakkethil Balakrishnan Sreekumar ◽  
Renuka . ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Western Ghats ◽  
Rapd Markers ◽  
Gene Diversity ◽  
Issr Markers ◽  
High Genetic Diversity ◽  
Western Ghats Of India ◽  
The Mean ◽  
Two Parameters ◽  
Two Populations

Calamus brandisii Becc. is one of the endemic slender rattans found in the Western Ghats of India. The genetic diversity of two main populations available in Kerala was investigated using 20 RAPD and 9 ISSR markers. Two parameters viz., gene diversity and genetic diversity within and among populations were analyzed. ISSR analysis showed quite high genetic diversity in Pandimotta compared to Bonacaud population whereas in RAPD markers both these populations were moderately diverse. The percentage of total genetic differentiation (Gst) among two populations is relatively higher than the mean Gst value indicating high genetic diversity within the populations. The genetic distance between these two populations was 0.1739 with ISSR markers and 0.1971 with RAPD markers. Because of its high genetic diversity, Pandimotta population can be treated as an important population of gene diversity with potentially useful genes. This may be included in the high priority reservoir for genetic conservation also.

scholarly journals Genetic, morphological and spatial characterization of two populations of Mabea fistulifera Mart. (Euphorbiaceae), in different successional stages

2005 ◽  
Vol 48 (2) ◽  
pp. 275-284 ◽  
Author(s):  
Maíra Figueiredo Goulart ◽  
Sérvio Pontes Ribeiro ◽  
Maria Bernadete Lovato
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Genetic Data ◽  
Ecological Traits ◽  
Spatial Characterization ◽  
Shannon’S Index ◽  
Successional Stages ◽  
Morphologic Data ◽  
Two Populations

Two populations of Mabea fistulifera (Euphorbiaceae) located at Rio Doce State Park (Minas Gerais, Brazil) in places characterized as in different successional stages, were investigated through genetic and ecological traits. Twenty randomly chosen individuals from each population had its genetic data assessed by 43 RAPD markers and were also evaluated through five morphologic traits and its spatial distribution. Both Shannon's index and AMOVA reveled that most of the genetic variation was found between individuals within populations and about 9% of variation was located between populations. Moreover, populations differed genetically, but presented similar values of genetic diversity. Morphologic data showed differences between populations. Significant correlations among matrix of genetic, geographic and morphologic distances were obtained considering both populations, and spatially closer individuals were more similar genetically, characterizing genetic substructured populations.

scholarly journals Molecular Evaluation of Three Populations of Farafra Sheep in Comparison to Ossimi and Rahmani Sheep Breeds

2020 ◽  
Vol 68 (6) ◽  
pp. 929-936
Author(s):  
Talaat Bashandy ◽  
Ahmed Hussein ◽  
Mohamed Solma ◽  
Ayman Kassab ◽  
Hatem Hamdon
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Rapd Markers ◽  
Rapd Marker ◽  
Sheep Breeds ◽  
Main Cluster ◽  
Ideal Approach ◽  
Different Populations ◽  
The Mean ◽  
Molecular Evaluation

Molecular markers are the most ideal approach to study genetic diversity. Consequently, we utilized both ISSR and RAPD markers to assess genetic diversity and relationships among three different populations of Farafra, Ossimi and Rahmani Egyptian sheep breeds. Both ISSR and RAPD gave moderate polymorphism 41.3% and 48.51%, respectively. Besides, this value was consistent with the moderate value of the mean of polymorphism information content (0.16 and 0.20, respectively). Farafra-F and Farafra-D populations had the highest similarity which was 0.92 for ISSR and 0.90 for the RAPD marker. Furthermore, ISSR and RAPD constructed dendrogram separated all the studied sheep into two main clusters. All the three populations of Farafra breed combined into one main cluster, while the second cluster contained both Rahmani and Ossimi breeds. The used molecular markers were able to discriminate among evaluated sheep and displayed that Farafra breed more closely related to Ossimi than Rahmani breed.

scholarly journals Development of random amplified polymorphic DNA markers for authentication of Croton tiglium Linn.

2017 ◽  
Vol 6 (3) ◽  
pp. 164-166
Author(s):  
Shweta Vekariya ◽  
◽  
Krushnkumar Taviad ◽  
RN Acharya RN ◽  
CN Harisha ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Rapd Markers ◽  
Ricinus Communis ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Natural Habitat ◽  
Drug Market ◽  
Croton Tiglium ◽  
Young Leaves

Background: Croton tiglium Linn., commonly known as Jayapala, in Ayurvedic pharmacopoeia, is wellknown for its purgative action. In the herbal raw drug market, seeds of Baliospermum montanum Blume., Ricinus communis Linn. and Croton roxburghii Wall. etc. are sold in the name of Jayapla seeds due to their morphological similarities. Hence their identification through molecular characters is need of the hour. Aim: Present study aims with the molecular characterization of young leaves of C. tiglium Linn. Materials & Methods: Fresh young leaves of C. tiglium Linn. were collected from its natural habitat Udupi, Karnnataka, during January 2017. It was used for molecular characterization and DNA fingerprints, by standard and most convenient Random Amplified Polymorphic DNA (RAPD) markers at Food testing laboratory, Junagadh Agriculture University, Gujarat, India. Results: All the primers gave good band patterns. Primer 5, 6, 8, 11 and 13 showed more number of light and bright bands matching characters with plant. Conclusion: Observed RAPD marker can be used to differentiate genuine as well as adulterated samples. The results may be used for the further research purposes and also required DNA Barcoding studies for further authentication.

scholarly journals Genetic diversity analysis in cashew (Anacardium occidentale L.) germplasm using RAPD marker

2019 ◽  
Vol 17 (4) ◽  
pp. 461-465
Author(s):  
T Bhadra ◽  
AZM Obaidullah ◽  
Mst Sabiha Sultana ◽  
M Ahmed ◽  
MM Islam
Keyword(s):  
Genetic Distance ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Climatic Conditions ◽  
Anacardium Occidentale ◽  
Cashew Nut ◽  
Major Cluster ◽  
The Family ◽  
Wide Potential

Anacardium occidentale L., commonly known as cashew nut, belongs to the family Anacardiaceae.  It is regarded as a high valued fruit nut crop world-wide. Potential of this economically important nut  is under-utilized in Bangladesh in spite of having all favorable agro-climatic conditions. The objective of the present investigation was to characterize six cashew accessions using Random Amplified Polymorphic DNA (RAPD) markers. Four random primers viz. OPE-02, OPE-18, OPK-03 and OPB-15 were used to amplify DNA segments. A total of 33 reproducible bands were obtained, out of which 11 were monomorphic and 22 were polymorphic. On average 74.12% polymorphism was observed. . Primers OPB-15 and OPK-03 yielded 100% polymorphism and OPE 02 and OPE 18 produced 33.33% and 63.16 % polymorphism, respectively. Cluster analysis revealed two main distinct groups, first group included GP-1 and the second consisted of five genotypes viz. GP-2, GP-3, GP-4, GP-5and GP-6 The major cluster- II was further subdivided into two minor clusters i.e. minor cluster- III and IV. Minor cluster- III contained only one genotype GP-4. Minor cluster- IV consists of four rest genotypes. The genetic distance between the groups was found low and varied from 0.002 to 0.0308. Maximum genetic distance was observed between GP-1 and GP-2cashew germplasm and minimum between GP-5 and GP-6. The low genetic distance which is unusual for this out crossing long-lived tree species, indicates the probability of having common ancestry among the germplasm or may be due to the use of a narrow range of populations for the investigation. J Bangladesh Agril Univ 17(4): 461–465, 2019

scholarly journals Characterization of Ocimum tenuiflorum (Linn.) Morpho-types Using RAPD Markers

2019 ◽  
Vol 11 (4) ◽  
pp. 417-420
Author(s):  
James T. OPOOLA ◽  
Matthew OZIEGBE
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Rapd Markers ◽  
Clustering Algorithm ◽  
Rapd Marker ◽  
Purple Pigment ◽  
Aromatic Plant ◽  
Mode Of Reproduction ◽  
Ocimum Tenuiflorum

Ocimum tenuiflorum Linn. (Lamiaceae) is an aromatic plant with a lot of potential medicinally, industrially and domestically. There are different morpho-types of O. tenuiflorum, with different shades of purple pigment on their leaves. Very few studies have been done to study the genetic variation among O. tenuiflorum morpho-types. Thus, in the present study, four O. tenuiflorum morpho-types that varied in pigmentation were characterised using RAPD marker as a molecular tool. Eight different primers collectively amplified 64 bands in the four morpho-types analysed. The RAPD technique revealed that the varied amount of purple pigment in O. tenuiflorum morpho-types is not due to environmental factor alone. The UPGMA clustering algorithm based on RAPD data grouped the four morpho-types into two major groups, with O. tenuiflorum (T2) with very light purple leaves and stem morpho-type, more related to O. tenuiflorum (T3) that have deep purple leaves and stem. The result was able to show the phylogenetic relationships within the species. The low level of genetic diversity observed in O. tenuiflorum can be attributed to the mode of reproduction and the reproductive biology of the species, due to the fact that they are likely to be natural hybrids produced through cross pollination.

scholarly journals Keragaman Genetik Beberapa Genotipe Teh Berdasarkan Penanda RAPD (Random Amplified Polymorphic DNA)

2014 ◽  
Vol 1 (1) ◽  
pp. 1 ◽  
Author(s):  
Budi Martono ◽  
Laba Udarno
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Camellia Sinensis ◽  
Genetic Similarity ◽  
Rapd Markers ◽  
Dna Marker ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Plant Breeding Program ◽  
Marker Loci

<p>Informasi keragaman genetik dan ketersediaan plasma nutfah teh (Camellia sinensis) diperlukan dalam perakitan varietas unggul. Keragaman genetik berdasarkan penanda DNA dapat memberikan hasil yang lebih konsisten karena tidak dipengaruhi lingkungan. Dalam penelitian ini sebanyak 9 genotipe teh dianalisis keragamannya menggunakan enam penanda RAPD (OPA 03, OPA 05, OPB 04, OPB 06, OPC 06, dan OPD 08). Penelitian dilakukan mulai bulan Maret sampai Mei 2013 di Laboratorium Terpadu Biotrop Bogor. Perhitungan koefisien kesamaan genetik dan analisis gerombol dilakukan dengan menggunakan perangkat lunak NTSYSpc versi 2.02. Sebanyak 54 lokus penanda RAPD berhasil diamplifikasi menggunakan enam primer dan 47 lokus di antaranya memiliki alel yang polimorfik (87,04%). Hasil analisis gerombol berdasarkan kesamaan genetiknya mengelompokkan 9 genotipe ke dalam enam kelompok. Empat kelompok (I, II, IV, V) masing-masing terdiri atas satu genotipe, sementara dua kelompok yang lain yaitu kelompok III dan VI masing-masing beranggotakan tiga dan dua genotipe.</p><p>Kata Kunci: Camellia sinensis, diversitas genetik, penanda RAPD</p><p>The availability of diverse tea (Camellia sinensis) germplasms as well as the information about their genetic diversity is required for plant breeding program. Genetic diversity analysis based on DNA marker is known to be more effective since the markers provide more consistent results. In this study, nine tea genotypes were evaluated for their genetic diversity using six Random Amplified Polymorphic DNA (RAPD) markers (OPA 03, OPA 05, OPB 04, OPB 06, OPC 06, and OPD 08). The study was conducted from March to May 2013 in the Integrated Laboratory of Biotrop Bogor. The estimation of genetic similarity and the cluster analysis were done using NTSYSpc version 2.02. Of the six RAPD markers used in this study, a total of 54 RAPD marker loci have been successfully amplified. In which, 47 loci (87.04%) were polymorphic and subsequently used for the evaluation of tea genotypes. The results of cluster analysis showed that those tea genotypes were clustered into six groups. Each of four groups (I, II, IV, V) consisted of only one genotype. Meanwhile, the other two groups (III and VI) had three and two genotypes, respectively.</p>

scholarly journals Genetic diversity of Aquilaria microcarpa Baill. in Kalimantan using RAPD Markers

2021 ◽  
Vol 914 (1) ◽  
pp. 012039
Author(s):  
M Qiptiyah ◽  
A Y P B C Widyatmoko ◽  
I L G Nurtjahjaningsih ◽  
I Prihatini
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Local Community ◽  
Geographic Distance ◽  
Iucn Red List ◽  
Private Allele ◽  
West Kalimantan ◽  
Seed Sources ◽  
Expected Heterozygosity ◽  
Two Populations

Abstract Aquilaria microcarpa is one of the agarwood producers species included in the endangered category based on the IUCN red list. Kalimantan is one of the natural distributions of this species. The high demand for agarwood causes this species to be commonly cultivated in community plantations. This study aimed to assess the genetic diversity of A. microcarpa using the RAPD markers. Forty-four leaf samples of A.microcarpa were collected from the local community in Gumbil (South Kalimantan) and Sanggau (West Kalimantan). The results showed that 9 out of 24 RAPD primers were stable in amplification, and polymorphic totally consisted of 49 polymorphic loci. The values of unbiased expected heterozygosity (uHE) were at a low level; they ranged between 0.152 (Gumbil II) to 0.249 (Gumbil I). The average genetic distance between Gumbil and Sanggau is 0.1733, related to their geographic distance. A private allele was only found at Gumbil at locus G18/540 and Sanggau at locus G18/550, respectively. A great DA value between Gumbil I and II showed that the plantations originated from different seed sources. AMOVA also verified the differences between the two populations. This finding has important implications in managing plantations and seeds transfer.

scholarly journals Discrimination and Genetic Diversity among Cultivated Olives of Greece Using RAPD Markers

2003 ◽  
Vol 128 (5) ◽  
pp. 741-746 ◽  
Author(s):  
N. Nikoloudakis ◽  
G. Banilas ◽  
F. Gazis ◽  
P. Hatzopoulos ◽  
J. Metzidakis
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Fruit Size ◽  
Similarity Index ◽  
Group Method ◽  
Poor Correlation ◽  
Pair Group ◽  
Electrophoretic Patterns

Random amplified polymorphic DNA (RAPD) markers were used to study the genetic diversity and to discriminate among 33 Greek olive (Olea europaea L.) cultivars. Three feral forms from Crete and five foreign cultivars recently introduced into Greece were also included. Nineteen primers were selected which produced 64 reproducible polymorphic bands in the 41 olive genotypes studied, with an average of 3.4 informative markers per primer. The RAPD markers resulted in 135 distinct electrophoretic patterns, with an average of 7.1 patterns per primer. Based on either unique or combined patterns, all genotypes could be identified. Genetic similarities between genotypes were estimated using the Dice similarity index and these indicated that a high degree of diversity exists within the Greek olive germplasm. Using the unweighted pair-group method (UPGMA) most cultivars were clustered into two main groups according to their fruit size or commercial use (table or olive oil). However, poor correlation was detected between clustering of cultivars and their principal area of cultivation. RAPD marker data were subjected to nonmetric multidimentional scaling (NMDS) which produced results similar to those of the UPGMA analysis. The results presented here contribute to a comprehensive understanding of cultivated Greek olive germplasm and provide information that could be important for cultural purposes and breeding programs.

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