Using31P magnetic resonance spectroscopy, creatine kinase (CK) reaction kinetics was assessed in the forearm flexor digitorum profundus muscle of healthy young ( n = 11, age 34.7 ± 5 yr) and older ( n = 20, age 73.5 ± 8 yr) subjects at rest, intermittent exercise at 20% maximum voluntary contraction (MVC), and 40% MVC. Exercise resulted in a significant increase in the average ratio of inorganic phosphate (Pi) to phosphocreatine (PCr) from resting values of 0.073 ± 0.031 (young) and 0.082 ± 0.037 (older) to 0.268 ± 0.140 (young, P < 0.01) and 0.452 ± 0.387 (older, P < 0.01) at 40% MVC. At 40% MVC, intracellular pH decreased significantly, from resting values of 7.08 ± 0.08 (young) and 7.08 ± 0.11 (older) to 6.84 ± 0.19 (young, P < 0.05) and to 6.75 ± 0.25 (older, P < 0.05). Average values of the pseudo-first-order reaction rate k (PCr→ATP) at rest were 0.07 ± 0.04 s−1 in the young and 0.07 ± 0.03 s−1 in the older group. At both exercise levels, the reaction rate constant increased compared with the resting value, but only the difference between the resting value and the 20% MVC value, which showed an 86% higher reaction rate constant in both groups, reached statistical significance ( P < 0.05). No difference in the reaction rate constant between the young and older groups was observed at either exercise level. As with k (PCr→ATP), the average phosphorus flux through the CK reaction increased during exercise at 20% MVC ( P < 0.05 in the older group) but decreased toward resting values at 40% MVC in both groups. The data in our study suggest that normal aging does not significantly affect the metabolic processes associated with the CK reaction.
Measurement of creatine kinase reaction rate in human brain using magnetization transfer image-selected in vivo
spectroscopy (MT-ISIS) and a volume 31
P/1
H radiofrequency coil in a clinical 3-T MRI system
