scholarly journals Optimization production conditions of photosynthetic purple bacteria biomass at pilot scale to remove sulphide from aquaculture pond

2018 ◽  
Vol 9 (2) ◽  
pp. 112-117
Author(s):  
Thi Lien Do ◽  
Thi To Uyen Do ◽  
Thi Nhi Cong Le ◽  
Phuong Ha Hoang ◽  
Thi Ngoc Mai Cung
Keyword(s):  
Biomass Production ◽  
Photosynthetic Bacteria ◽  
Low Cost ◽  
Purple Bacteria ◽  
Pilot Scale ◽  
Coastal Zones ◽  
Natural Lighting ◽  
Sulfur Bacteria ◽  
Aquaculture Ponds ◽  
Quang Ninh

For the purpose of sulphide removal in aquaculture ponds, three strains (name: TH21, QN71, QN51) were isolated and selected with the highest sulphide removal activity from Thanh Hoa and Quang Ninh coastal zones. These strains have identified and tested in a number of aquaculture ponds in different areas with good water quality results. With the objective of purple non sulfur bacteria biomass production containing 3 selected strains for wide application and suitable price for farmers, in this study, we study on optimum conditions of mixed purple non sulfur bacteria biomass production at pilot scale. The results showed that the sources of substrates were soybean meal (1g/l) and acetate (0.5g/l). These substrates are low cost, easy to find, convenient in large culture. The mixture of photosynthetic bacteria can be cultured in glass tanks, under micro aerobic and natural lighting conditions that produce highly concentrated photosynthetic bacteria and lowest rest media. Nhằm mục tiêu xử lý sulphide trong môi trường nuôi trồng thủy sản, chúng tôi đã phân lập và lựa chọn được ba chủng vi khuẩn tía quang hợp có khả năng loại bỏ sulphide cao nhất ký hiệu TH21, QN71, QN52 từ các vùng ven biển Thanh Hóa và Quảng Ninh. Các chủng này đã được định loại và thử nghiệm tại một số ao nuôi thủy sản ở các vùng khác nhau thu được kết quả tốt về chất lượng nước. Để tạo chế phẩm vi khuẩn tía quang hợp từ 3 chủng lựa chọn được ứng dụng rộng rãi và có giá thành phù hợp cho nông hộ, trong nghiên cứu này, chúng tôi nghiên cứu tối ưu hóa các điều kiện sản xuất sinh khối hỗn hợp 3 chủng vi khuẩn tía quang hợp ở quy mô pilot. Kết quả cho thấy đã tìm kiếm được nguồn cơ chất là bột đậu tương (1g/l) và acetate (0.5g/l) là những chất có giá thành thấp, dễ tìm kiếm, thuận tiện trong nhân nuôi ở quy mô lớn. Hỗn hợp vi khuẩn tía quang hợp có thể nuôi trong các bể kính, ở điều kiện vi hiếu khí, có ánh sáng chiếu tự nhiên có thể sản xuất được chế phẩm vi khuẩn tía quang hợp có mật độ cao, cơ chất còn lại sau sản xuất là ít nhất.

scholarly journals A Cyanobacteria-Based Biofilm System for Advanced Brewery Wastewater Treatment

2020 ◽  
Vol 11 (1) ◽  
pp. 174
Author(s):  
Konstantinos P. Papadopoulos ◽  
Christina N. Economou ◽  
Athanasia G. Tekerlekopoulou ◽  
Dimitris V. Vayenas
Keyword(s):  
Wastewater Treatment ◽  
Biomass Production ◽  
Low Cost ◽  
Oxygen Demand ◽  
Dry Weight ◽  
Surface Hydrophobicity ◽  
Reactor Performance ◽  
Brewery Wastewater ◽  
Kjeldahl Nitrogen ◽  
Supporting Materials

Algal/cyanobacterial biofilm photobioreactors provide an alternative technology to conventional photosynthetic systems for wastewater treatment based on high biomass production and easy biomass harvesting at low cost. This study introduces a novel cyanobacteria-based biofilm photobioreactor and assesses its performance in post-treatment of brewery wastewater and biomass production. Two different supporting materials (glass/polyurethane) were tested to investigate the effect of surface hydrophobicity on biomass attachment and overall reactor performance. The reactor exhibited high removal efficiency (over 65%) of the wastewater’s pollutants (chemical oxygen demand, nitrate, nitrite, ammonium, orthophosphate, and total Kjeldahl nitrogen), while biomass per reactor surface reached 13.1 and 12.8 g·m−2 corresponding to 406 and 392 mg·L−1 for glass and polyurethane, respectively, after 15 days of cultivation. The hydrophilic glass surface favored initial biomass adhesion, although eventually both materials yielded complete biomass attachment, highlighting that cell-to-cell interactions are the dominant adhesion mechanism in mature biofilms. It was also found that the biofilm accumulated up to 61% of its dry weight in carbohydrates at the end of cultivation, thus making the produced biomass a suitable feedstock for bioethanol production.

scholarly journals Growth and biochemical composition of Chlorella vulgaris in different growth media

2013 ◽  
Vol 85 (4) ◽  
pp. 1427-1438 ◽  
Author(s):  
MATHIAS A. CHIA ◽  
ANA T. LOMBARDI ◽  
MARIA DA GRACA G. MELAO
Keyword(s):  
Biomass Production ◽  
Chlorella Vulgaris ◽  
Biochemical Composition ◽  
Physiological Response ◽  
Low Cost ◽  
Cost Effective ◽  
Growth Conditions ◽  
Growth Media ◽  
Continuous Cultures ◽  
The Cost

The need for clean and low-cost algae production demands for investigations on algal physiological response under different growth conditions. In this research, we investigated the growth, biomass production and biochemical composition of Chlorella vulgaris using semi-continuous cultures employing three growth media (LC Oligo, Chu 10 and WC media). The highest cell density was obtained in LC Oligo, while the lowest in Chu medium. Chlorophyll a, carbohydrate and protein concentrations and yield were highest in Chu and LC Oligo media. Lipid class analysis showed that hydrocarbons (HC), sterol esthers (SE), free fatty acids (FFA), aliphatic alcohols (ALC), acetone mobile polar lipids (AMPL) and phospholipids (PL) concentrations and yields were highest in the Chu medium. Triglyceride (TAG) and sterol (ST) concentrations were highest in the LC Oligo medium. The results suggested that for cost effective cultivation, LC Oligo medium is the best choice among those studied, as it saved the cost of buying vitamins and EDTA associated with the other growth media, while at the same time resulted in the best growth performance and biomass production.

Enrichment of highly settleable microalgal consortia in mixed cultures for effluent polishing and low-cost biomass production

2017 ◽  
Vol 125 ◽  
pp. 11-22 ◽  
Author(s):  
Yuansheng Hu ◽  
Xiaodi Hao ◽  
Mark van Loosdrecht ◽  
Huiqin Chen
Keyword(s):  
Biomass Production ◽  
Low Cost ◽  
Mixed Cultures

Novel biofilm reactor for denitrification of municipal wastewater

2018 ◽  
Vol 78 (7) ◽  
pp. 1566-1575 ◽  
Author(s):  
S. S. Rathnaweera ◽  
B. Rusten ◽  
K. Korczyk ◽  
B. Helland ◽  
E. Rismyhr
Keyword(s):  
Carbon Source ◽  
Municipal Wastewater ◽  
Low Cost ◽  
Oxygen Demand ◽  
Pilot Scale ◽  
Biofilm Reactor ◽  
Treated Wastewater ◽  
Removal Rates ◽  
Good Filter ◽  
Solids Removal

Abstract A pilot-scale CFIC® (continuous flow intermittent cleaning) reactor was run in anoxic conditions to study denitrification of wastewater. The CFIC process has already proven its capabilities for biological oxygen demand removal with a small footprint, less energy consumption and low cost. The present study focused on the applicability for denitrification. Both pre-denitrification (pre-DN) and post-denitrification (post-DN) were tested. A mixture of primary treated wastewater and nitrified wastewater was used for pre-DN and nitrified wastewater with ethanol as a carbon source was used for post-DN. The pre-DN process was carbon limited and removal rates of only 0.16 to 0.74 g NOx-N/m²-d were obtained. With post-DN and an external carbon source, 0.68 to 2.2 g NO3-Neq/m²-d removal rates were obtained. The carrier bed functioned as a good filter for both the larger particles coming with influent water and the bio-solids produced in the reactor. Total suspended solids removal in the reactor varied from 20% to 78% (average 45%) during post-DN testing period and 9% to 70% (average 29%) for pre-DN. The results showed that the forward flow washing improves both the DN function and filtration ability of the reactor.

Pilot-scale process development for low-cost production of a thermostable biodiesel refining enzyme in Escherichia coli

2018 ◽  
Vol 41 (4) ◽  
pp. 555-564 ◽  
Author(s):  
Florencia Eberhardt ◽  
Andres Aguirre ◽  
Luciana Paoletti ◽  
Guillermo Hails ◽  
Mauricio Braia ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Process Development ◽  
Low Cost ◽  
Pilot Scale

scholarly journals Treatment and reuse of coalmine wastewater in Vietnam: application of microfiltration

2018 ◽  
Vol 53 (3) ◽  
pp. 133-142 ◽  
Author(s):  
H. T. T. Dang ◽  
H. D. Tran ◽  
S. H. Tran ◽  
M. Sasakawa ◽  
R. M. Narbaitz
Keyword(s):  
Pilot Scale ◽  
High Quality ◽  
Polyaluminum Chloride ◽  
Slaked Lime ◽  
Scale Test ◽  
Pre Treatment ◽  
Coated Sand ◽  
Quang Ninh ◽  
Wastewater Treatment Systems ◽  
Technical Regulations

Abstract Due to stringent local regulations and adverse environmental impacts, Vietnamese coal mining industries are under pressure to reuse the large volume of wastewater they produce. To this end, the aim of this study was to add microfiltration (MF) membranes after the conventional Vietnamese coalmine wastewater treatment systems (coagulation/sedimentation/filtration) to assess the feasibility of effluent reuse. The pilot-scale test was performed at a coalmine plant located in Quang Ninh province, Vietnam. Results indicate that precipitation with slaked lime (Ca(OH)2) and polyaluminum chloride (PACl) followed by sand filtration are important pre-treatment steps, prior to microfiltration. To achieve high Mn removals the sand in the filter had to be replaced by a KMnO4 coated sand. The MF membrane produced a stable and high quality effluent that meets the Vietnamese National Technical Regulations for Drinking Water quality (Fe < 0.5 mg/L, Mn < 0.3 mg/L, hardness <350 mg/L as CaCO3). Complete membrane recovery was achieved by sequential 24 h soakings in NaOCl, citric acid and a surfactant.

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