Effect of hydrogen peroxide on amino acid concentrations in bovine retina and vitreous humor, ex vivo

SummaryEchis colorata bites cause impairment of platelet aggregation and hemostatic disorders. The mechanism by which the snake venom inhibits platelet aggregation was studied. Upon fractionation, aggregation impairment activity and L-amino acid oxidase activity were similarly separated from the crude venom, unlike other venom enzymes. Preparations of L-amino acid oxidase from E.colorata and from Crotalus adamanteus replaced effectively the crude E.colorata venom in impairment of platelet aggregation. Furthermore, different treatments known to inhibit L-amino acid oxidase reduced in parallel the oxidase activity and the impairment potency of both the venom and the enzyme preparation. H2O2 mimicked characteristically the impairment effects of L-amino acid oxidase and the venom. Catalase completely abolished the impairment effects of the enzyme and the venom. It is concluded that hydrogen peroxide formed by the venom L-amino acid oxidase plays a role in affecting platelet aggregation and thus could contribute to the extended bleeding typical to persons bitten by E.colorata.

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Growth, carcass traits, and plasma amino acid concentrations of gilts fed low-protein diets supplemented with amino acids including histidine, isoleucine, and valine

Journal of Animal Science ◽

10.2527/2003.8161529x ◽

2003 ◽

Vol 81 (6) ◽

pp. 1529 ◽

Cited By ~ 62

Author(s):

J. L. Figueroa ◽

A. J. Lewis ◽

P. S. Miller ◽

R. L. Fischer ◽

R. M. Diedrichsen

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Carcass Traits ◽

Plasma Amino Acid ◽

Low Protein ◽

Protein Diets ◽

Amino Acid Concentrations

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Effect of Excess Metabolizable Protein Supplementation from Corn Gluten Meal or Soybean Meal on Plasma Amino Acid Concentrations of Beef Cows Consuming Low Quality Forage

10.31274/ans_air-180814-412 ◽

2016 ◽

Author(s):

Taylor Grussing ◽

Allison Meyer ◽

Patrick J. Gunn

Keyword(s):

Amino Acid ◽

Soybean Meal ◽

Beef Cows ◽

Protein Supplementation ◽

Plasma Amino Acid ◽

Corn Gluten Meal ◽

Metabolizable Protein ◽

Corn Gluten ◽

Amino Acid Concentrations

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Protein Source on Plasma Amino Acid Concentrations In Older Adults

Case Medical Research ◽

10.31525/ct1-nct04243395 ◽

2020 ◽

Author(s):

Keyword(s):

Older Adults ◽

Amino Acid ◽

Protein Source ◽

Plasma Amino Acid ◽

Amino Acid Concentrations

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An ESR study of the peroxidase reaction catalyzed by human methaemoglobin and methaemoglobin-haptoglobin complex

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19910923 ◽

1991 ◽

Vol 56 (4) ◽

pp. 923-932

Author(s):

Jana Stejskalová ◽

Pavel Stopka ◽

Zdeněk Pavlíček

Keyword(s):

Hydrogen Peroxide ◽

Ascorbic Acid ◽

Amino Acid ◽

Peroxidase Activity ◽

Amino Acid Analysis ◽

Superoxide Radical ◽

Esr Spectra ◽

The Other ◽

Delocalized Electron

The ESR spectra of peroxidase systems of methaemoglobin-ascorbic acid-hydrogen peroxide and methaemoglobin-haptoglobin complex-ascorbic acid-hydrogen peroxide have been measured in the acetate buffer of pH 4.5. For the system with methaemoglobin an asymmetrical signal with g ~ 2 has been observed which is interpreted as the perpendicular region of anisotropic spectrum of superoxide radical. On the other hand, for the system with methaemoglobin-haptoglobin complex the observed signal with g ~ 2 is symmetrical and is interpreted as a signal of delocalized electron. After realization of three repeatedly induced peroxidase processes the ESR signal of the perpendicular part of anisotropic spectrum of superoxide radical is distinctly diminished, whereas the signal of delocalized electron remains practically unchanged. An amino acid analysis of methaemoglobin along with results of the ESR measurements make it possible to derive a hypothesis about the role of haptoglobin in increasing of the peroxidase activity of methaemoglobin.

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