In Vivo μMRI-Based Finite Element and Morphological Analyses of Tibial Trabecular Bone in Eugonadal and Hypogonadal Men Before and After Testosterone Treatment

A finite element method to simulate the formation of an interconnected trabecular bone microstructure oriented with respect to applied in vivo mechanical forces is introduced and quantitatively compared to experimental data from a hydraulic bone chamber implant model. Randomly located 45 μm mineralized nodules were used as the initial condition for the model simulations to represent an early stage of intramembranous bone formation. Boundary conditions were applied consistent with the mechanical environment provided by the in vivo bone chamber model. A two-dimensional repair simulation algorithm that incorporated strain energy density (SED), SED gradient, principal strain, or principal strain gradient as the local objective criterion was utilized to simulate the formation of an oriented trabecular bone microstructure. The simulation solutions were convergent, unique, and relatively insensitive to the assumed initial distribution of mineralized nodules. Model predictions of trabecular bone morphology and anisotropy were quantitatively compared to experimental results. All simulations produced structures that qualitatively resembled oriented trabecular bone. However, only simulations utilizing a gradient objective criterion yielded results quantitatively similar to in vivo observations. This simulation approach coupled with an experimental model that delivers controlled in vivo mechanical stimuli can be utilized to study the relationship between physical factors and microstructural adaptation during bone repair.

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Potential of in vivo MRI-based nonlinear finite-element analysis for the assessment of trabecular bone post-yield properties

Medical Physics ◽

10.1118/1.4802085 ◽

2013 ◽

Vol 40 (5) ◽

pp. 052303 ◽

Cited By ~ 11

Author(s):

Ning Zhang ◽

Jeremy F. Magland ◽

Chamith S. Rajapakse ◽

Yusuf A. Bhagat ◽

Felix W. Wehrli

Keyword(s):

Finite Element Analysis ◽

Finite Element ◽

Trabecular Bone ◽

Nonlinear Finite Element Analysis ◽

Nonlinear Finite Element ◽

Element Analysis

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Assessment of trabecular bone strength at in vivo CT imaging with space-variant hysteresis and finite element modelling

2016 IEEE 13th International Symposium on Biomedical Imaging (ISBI) ◽

10.1109/isbi.2016.7493404 ◽

2016 ◽

Cited By ~ 2

Author(s):

Cheng Chen ◽

Ryan E. Amelon ◽

Anneliese Heiner ◽

Punam K. Saha

Keyword(s):

Finite Element ◽

Trabecular Bone ◽

Bone Strength ◽

Finite Element Modelling ◽

Ct Imaging ◽

Element Modelling ◽

Space Variant

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In Vivo Assessment of Architecture and Micro-Finite Element Analysis Derived Indices of Mechanical Properties of Trabecular Bone in the Radius

Osteoporosis International ◽

10.1007/s198-002-8332-0 ◽

2002 ◽

Vol 13 (1) ◽

pp. 6-17 ◽

Cited By ~ 43

Author(s):

B. van Rietbergen ◽

G. von Ingersleben ◽

C. Chesnut ◽

B. MacDonald ◽

H. K. Genant ◽

...

Keyword(s):

Mechanical Properties ◽

Finite Element Analysis ◽

Finite Element ◽

Trabecular Bone ◽

Element Analysis ◽

In Vivo Assessment

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Validation of an in vivo model for monitoring trabecular bone quality changes using finite element analysis.

10.18297/etd/721 ◽

2005 ◽

Author(s):

Boon Kam

Keyword(s):

Finite Element Analysis ◽

Finite Element ◽

Trabecular Bone ◽

Bone Quality ◽

In Vivo Model ◽

Quality Changes ◽

Element Analysis ◽

Trabecular Bone Quality

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Radiolabeled r-Hirudin as a Measure of Thrombin Activity at, or within, the Rabbit Aorta Wall In Vitro and In Vivo

Thrombosis and Haemostasis ◽

10.1055/s-0038-1642467 ◽

1994 ◽

Vol 71 (04) ◽

pp. 499-506 ◽

Cited By ~ 10

Author(s):

Mark W C Hatton ◽

Bonnie Ross-Ouellet

Keyword(s):

Rabbit Aorta ◽

Balloon Injury ◽

Recombinant Hirudin ◽

Aorta Wall ◽

Thrombin Activity ◽

Before And After ◽

The Matrix

SummaryThe behavior of 125I-labeled recombinant hirudin towards the uninjured and de-endothelialized rabbit aorta wall has been studied in vitro and in vivo to determine its usefulness as an indicator of thrombin activity associated with the aorta wall. Thrombin adsorbed to either sulfopropyl-Sephadex or heparin-Sepharose bound >95% of 125I-r-hirudin and the complex remained bound to the matrix. Binding of 125I-r-hirudin to the exposed aorta subendothelium (intima-media) in vitro was increased substantially if the tissue was pre-treated with thrombin; the quantity of l25I-r-hirudin bound to the de-endothelialized intima-media (i.e. balloon-injured in vitro) correlated positively with the quantity of bound 131I-thrombin (p <0.01). Aortas balloon-injured in vivo were measured for thrombin release from, and binding of 125I-r-hirudin to, the de-endothelialized intimal surface in vitro; 125I-r-hirudin binding correlated with the amount of active thrombin released (p <0.001). Uptake of 125I-r-hirudin by the aorta wall in vivo was proportional to the uptake of 131I-fibrinogen (as an indicator of thrombin activity) before and after balloon injury. After 30 min in the circulation, specific 125I-r-hirudin binding to the uninjured and de-endo- thelialized (at 1.5 h after injury) aorta wall was equivalent to 3.4 (± 2.5) and 25.6 (±18.1) fmol of thrombin/cm2 of intima-media, respectively. Possibly, only hirudin-accessible, glycosaminoglycan-bound thrombin is measured in this way.

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The Effect of Active Site-inhibited Factor VIIa on Tissue Factor-initiated Coagulation Using Platelets before and after Aspirin Administration

Thrombosis and Haemostasis ◽

10.1055/s-0038-1657715 ◽

1997 ◽

Vol 78 (04) ◽

pp. 1202-1208 ◽

Cited By ~ 18

Author(s):

Marianne Kjalke ◽

Julie A Oliver ◽

Dougald M Monroe ◽

Maureane Hoffman ◽

Mirella Ezban ◽

...

Keyword(s):

Tissue Factor ◽

Active Site ◽

Large Scale ◽

Thrombin Generation ◽

Factor Viia ◽

Dependent Manner ◽

Antithrombotic Agent ◽

Before And After ◽

Ic50 Values

SummaryActive site-inactivated factor VIIa has potential as an antithrombotic agent. The effects of D-Phe-L-Phe-L-Arg-chloromethyl ketone-treated factor VIla (FFR-FVIIa) were evaluated in a cell-based system mimicking in vivo initiation of coagulation. FFR-FVIIa inhibited platelet activation (as measured by expression of P-selectin) and subsequent large-scale thrombin generation in a dose-dependent manner with IC50 values of 1.4 ± 0.8 nM (n = 8) and 0.9 ± 0.7 nM (n = 7), respectively. Kd for factor VIIa binding to monocytes ki for FFR-FVIIa competing with factor VIIa were similar (11.4 ± 0.8 pM and 10.6 ± 1.1 pM, respectively), showing that FFR-FVIIa binds to tissue factor in the tenase complex with the same affinity as factor VIIa. Using platelets from volunteers before and after ingestion of aspirin (1.3 g), there were no significant differences in the IC50 values of FFR-FVIIa [after aspirin ingestion, the IC50 values were 1.7 ± 0.9 nM (n = 8) for P-selectin expression, p = 0.37, and 1.4 ± 1.3 nM (n = 7) for thrombin generation, p = 0.38]. This shows that aspirin treatment of platelets does not influence the inhibition of tissue factor-initiated coagulation by FFR-FVIIa, probably because thrombin activation of platelets is not entirely dependent upon expression of thromboxane A2.

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Determination and Treatment of Disorders of Primary Haemostasis

Hämostaseologie ◽

10.1055/s-0038-1660415 ◽

1999 ◽

Vol 19 (04) ◽

pp. 168-175 ◽

Cited By ~ 6

Author(s):

M. Weippert-Kretschmer ◽

V. Kretschmer

Keyword(s):

Platelet Function ◽

Bleeding Risk ◽

Bleeding Complications ◽

Bleeding Tendency ◽

Platelet Counts ◽

Platelet Function Disorders ◽

Perioperative Bleeding ◽

Before And After ◽

Low Sensitivity

SummaryPerioperative bleeding complications due to disorders of primary haemostasis are often underestimated. Routine determination of primary haemostasis is still problematic. The in vivo bleeding time (BT) shows low sensitivity and high variability. In this contribution the results and experiences with the IVBT having been obtained in various studies and during 10 years of routine use are reported. Patients and Methods: Blood donors before and after ASA ingestion, patients with thrombocytopenia as well as congenital and acquired platelet function disorders. Monitoring of desmopressin efficacy. IVBT with Thrombostat 4000 (tests with CaCl2 = TST-CaCl2 and ADP = TST-ADP) and PFA-100 (test cartridges with epinephrine = PFA-EPI and ADP = PFA-ADP). Results and Conclusions: IVBT becomes abnormal with platelet counts <100,000/μl. With platelet counts <50,000/μl the results are mostly outside the methodical range. IVBT proved clearly superior to BT in von Willebrand syndrome (vWS). All 16 patients with vWS were detected by PFA-EPI, whereas with BT 7 of 10 patients with moderate and 1 of 6 patients with mild forms of vWS were spotted. The majority of acquired and congenital platelet function disorders with relevant bleeding tendency were detectable by IVBT. Sometimes diagnostic problems arose in case of storage pool defect. Four to 12 h after ingestion of a single dose of 100 mg ASA the TST-CaCl2 became abnormal in all cases, the PFA-EPI only in 80%. However, the ASA sensitivity of TST-CaCl2 proved even too high when looking for perioperative bleeding complications in an urological study. Therefore, the lower ASS sensitivity of the PFA-100 seems to be rather advantageous for the estimation of a real bleeding risk. The good efficacy of desmopressin in the majority of cases with mild thrombocytopenia, congenital and acquired platelet function disorders and even ASS-induced platelet dysfunction could be proven by means of the IVBT. Thus IVBT may help to increase the reliability of the therapy. However, the IVBT with the PFA-100 is not yet fully developed. Nevertheless, routine use can be recommended when special methodical guidelines are followed.

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Аpplying of high-frequency monopolar diathermocoagulation in the treatment of chronic pulpitis

Medical alphabet ◽

10.33667/2078-5631-2020-12-40-42 ◽

2020 ◽

Vol 1 (12) ◽

pp. 40-42

Author(s):

F. Yu. Daurova ◽

D. I. Tomaeva ◽

S. V. Podkopaeva ◽

Yu. A. Taptun

Keyword(s):

Root Canal ◽

High Frequency ◽

Antibacterial Effect ◽

Comparison Group ◽

Poor Quality ◽

Endodontic Treatment ◽

Root Canals ◽

The Third ◽

Before And After

Relevance: the reason for the development of complications in endodontic treatment is poor-quality instrumental treatment root canals.Aims: a study of the animicrobial action and clinical efficacy of high-frequency monopolar diathermocoagulation in the treatment of chronic forms of pulpitis.Materials and methods: 102 patients with various chronic forms of pulpitis were divided into three groups of 34 patients each. In the first two groups, high-frequency monopolar diathermocoagulation was used in endodontic treatment in different modes. In the third group, endodontic treatment was carried out without the use of diathermocoagulation (comparison group). The root canal microflora in chronic pulpitis in vivo was studied twice-before and after diathermocoagulation.Results: it was established that high-frequency monopolar diathermocoagulation in the effect mode is 3, power is 4 (4.1 W) and effect is 4, power is 4 (5.4 W) with an exposure time of 3 seconds, it has a pronounced antibacterial effect on all presented pathogenic microflora obtained from the root canals of the teeth.

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