In vivo fast variable focus photoacoustic microscopy using an electrically tunable lens

AbstractPhotoacoustic microscopy is an in vivo imaging technology based on the photoacoustic effect. It is widely used in various biomedical studies because it can provide high-resolution images while being label-free, safe, and harmless to biological tissue. Polygon-scanning is an effective scanning method in photoacoustic microscopy that can realize fast imaging of biological tissue with a large field of view. However, in polygon-scanning, fluctuations of the rotating motor speed and the geometric error of the rotating mirror cause image distortions, which seriously affect the photoacoustic-microscopy imaging quality. To improve the image quality of photoacoustic microscopy using polygon-scanning, an image correction method is proposed based on accurate ultrasound positioning. In this method, the photoacoustic and ultrasound imaging data of the sample are simultaneously obtained, and the angle information of each mirror used in the polygon-scanning is extracted from the ultrasonic data to correct the photoacoustic images. Experimental results show that the proposed method can significantly reduce image distortions in photoacoustic microscopy, with the image dislocation offset decreasing from 24.774 to 10.365 μm.

Download Full-text

In vivo functional chronic imaging of a small animal model using optical-resolution photoacoustic microscopy

Medical Physics ◽

10.1118/1.3137572 ◽

2009 ◽

Vol 36 (6Part1) ◽

pp. 2320-2323 ◽

Cited By ~ 42

Author(s):

Song Hu ◽

Konstantin Maslov ◽

Lihong V. Wang

Keyword(s):

Animal Model ◽

Optical Resolution ◽

Small Animal ◽

Photoacoustic Microscopy ◽

Small Animal Model

Download Full-text

Subwavelength-resolution label-free photoacoustic microscopy of optical absorption in vivo

Optics Letters ◽

10.1364/ol.35.003195 ◽

2010 ◽

Vol 35 (19) ◽

pp. 3195 ◽

Cited By ~ 154

Author(s):

Chi Zhang ◽

Konstantin Maslov ◽

Lihong V. Wang

Keyword(s):

Optical Absorption ◽

Photoacoustic Microscopy ◽

Label Free ◽

Subwavelength Resolution

Download Full-text

Reflection-mode in vivo photoacoustic microscopy with subwavelength lateral resolution

Biomedical Optics Express ◽

10.1364/boe.5.004235 ◽

2014 ◽

Vol 5 (12) ◽

pp. 4235 ◽

Cited By ~ 45

Author(s):

Wei Song ◽

Wei Zheng ◽

Ruimin Liu ◽

Riqiang Lin ◽

Hongtao Huang ◽

...

Keyword(s):

Lateral Resolution ◽

Photoacoustic Microscopy ◽

Reflection Mode

Download Full-text

Transcranial Imaging of Functional Cerebral Hemodynamic Changes in Single Blood Vessels using in vivo Photoacoustic Microscopy

Journal of Cerebral Blood Flow & Metabolism ◽

10.1038/jcbfm.2012.42 ◽

2012 ◽

Vol 32 (6) ◽

pp. 938-951 ◽

Cited By ~ 47

Author(s):

Lun-De Liao ◽

Chin-Teng Lin ◽

Yen-Yu I Shih ◽

Timothy Q Duong ◽

Hsin-Yi Lai ◽

...

Keyword(s):

Blood Vessels ◽

Cerebral Blood Volume ◽

Hemoglobin Concentration ◽

Photoacoustic Microscopy ◽

Functional Changes ◽

Sagittal Sinus ◽

Microscopy Technique ◽

Initial Dip ◽

Transcranial Imaging

Optical imaging of changes in total hemoglobin concentration ( HbT), cerebral blood volume ( CBV), and hemoglobin oxygen saturation ( SO 2) provides a means to investigate brain hemodynamic regulation. However, high-resolution transcranial imaging remains challenging. In this study, we applied a novel functional photoacoustic microscopy technique to probe the responses of single cortical vessels to left forepaw electrical stimulation in mice with intact skulls. Functional changes in HbT, CBV, and SO 2 in the superior sagittal sinus and different-sized arterioles from the anterior cerebral artery system were bilaterally imaged with unambiguous 36 × 65- μm2 spatial resolution. In addition, an early decrease of SO 2 in single blood vessels during activation (i.e., ‘the initial dip’) was observed. Our results indicate that the initial dip occurred specifically in small arterioles of activated regions but not in large veins. This technique complements other existing imaging approaches for the investigation of the hemodynamic responses in single cerebral blood vessels.

Download Full-text

High-speed high-resolution laser diode-based photoacoustic microscopy for in vivo microvasculature imaging

Visual Computing for Industry Biomedicine and Art ◽

10.1186/s42492-020-00067-5 ◽

2021 ◽

Vol 4 (1) ◽

Author(s):

Xiufeng Li ◽

Victor T C Tsang ◽

Lei Kang ◽

Yan Zhang ◽

Terence T W Wong

Keyword(s):

High Resolution ◽

High Speed ◽

High Performance ◽

Continuous Wave ◽

Signal To Noise Ratio ◽

Cost Effective ◽

High Signal ◽

Photoacoustic Microscopy ◽

Mouse Ear

AbstractLaser diodes (LDs) have been considered as cost-effective and compact excitation sources to overcome the requirement of costly and bulky pulsed laser sources that are commonly used in photoacoustic microscopy (PAM). However, the spatial resolution and/or imaging speed of previously reported LD-based PAM systems have not been optimized simultaneously. In this paper, we developed a high-speed and high-resolution LD-based PAM system using a continuous wave LD, operating at a pulsed mode, with a repetition rate of 30 kHz, as an excitation source. A hybrid scanning mechanism that synchronizes a one-dimensional galvanometer mirror and a two-dimensional motorized stage is applied to achieve a fast imaging capability without signal averaging due to the high signal-to-noise ratio. By optimizing the optical system, a high lateral resolution of 4.8 μm has been achieved. In vivo microvasculature imaging of a mouse ear has been demonstrated to show the high performance of our LD-based PAM system.

Download Full-text