Integrated Analysis of miRNA and mRNA Expression in Childhood Medulloblastoma Compared with Neural Stem Cells

AbstractEpigenetic mechanisms which play an essential role in normal developmental processes, such as self-renewal and fate specification of neural stem cells (NSC) are also responsible for some of the changes in the glioblastoma (GBM) genome. Here we develop a strategy to compare the epigenetic and transcriptional make-up of primary GBM cells (GIC) with patient-matched expanded potential stem cell (EPSC)-derived NSC (iNSC). Using a comparative analysis of the transcriptome of syngeneic GIC/iNSC pairs, we identify a glycosaminoglycan (GAG)-mediated mechanism of recruitment of regulatory T cells (Tregs) in GBM. Integrated analysis of the transcriptome and DNA methylome of GBM cells identifies druggable target genes and patient-specific prediction of drug response in primary GIC cultures, which is validated in 3D and in vivo models. Taken together, we provide a proof of principle that this experimental pipeline has the potential to identify patient-specific disease mechanisms and druggable targets in GBM.

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Bioinspired Nanocomplex for Spatiotemporal Imaging of Sequential mRNA Expression in Differentiating Neural Stem Cells

ACS Nano ◽

10.1021/nn505047n ◽

2014 ◽

Vol 8 (12) ◽

pp. 12386-12396 ◽

Cited By ~ 17

Author(s):

Zhe Wang ◽

Ruili Zhang ◽

Zhongliang Wang ◽

He-Fang Wang ◽

Yu Wang ◽

...

Keyword(s):

Stem Cells ◽

Neural Stem Cells ◽

Mrna Expression

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SmartFlareTM is a reliable method for assessing mRNA expression in single neural stem cells

World Journal of Stem Cells ◽

10.4252/wjsc.v13.i12.1918 ◽

2021 ◽

Vol 13 (12) ◽

pp. 1918-1927

Author(s):

Andrea Diana ◽

Maria Dolores Setzu ◽

Zaal Kokaia ◽

Roxana Nat ◽

Cristina Maxia ◽

...

Keyword(s):

Stem Cells ◽

Neural Stem Cells ◽

Mrna Expression ◽

Reliable Method

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Neurotrophins facilitate neuronal differentiation of cultured neural stem cells via induction of mRNA expression of basic helix-loop-helix transcription factors Mash1 and Math1

Journal of Neuroscience Research ◽

10.1002/jnr.10532 ◽

2003 ◽

Vol 71 (5) ◽

pp. 648-658 ◽

Cited By ~ 52

Author(s):

Hisanori Ito ◽

Aki Nakajima ◽

Hiroshi Nomoto ◽

Shoei Furukawa

Keyword(s):

Stem Cells ◽

Transcription Factors ◽

Neural Stem Cells ◽

Mrna Expression ◽

Neuronal Differentiation ◽

Basic Helix Loop Helix ◽

Helix Loop Helix

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LncRNA and mRNA Expression Profiles in Methylprednisolone Stimulated Neural Stem Cells

Frontiers in Neuroscience ◽

10.3389/fnins.2021.669224 ◽

2021 ◽

Vol 15 ◽

Author(s):

Yong Tang ◽

Zhongyu Xie ◽

Mengjun Ma ◽

Kaidi Duan ◽

Yuxi Li ◽

...

Keyword(s):

Stem Cells ◽

Neural Stem Cells ◽

Mrna Expression ◽

Expression Profiles ◽

Differentially Expressed ◽

Akt Signaling Pathway ◽

External Stimulation ◽

Cord Injury ◽

And Migration ◽

Insight Into

Spinal cord injury (SCI) is a devastating neurological disorder that affects thousands of individuals each year. Previously, our study in non-human primates with SCI demonstrated that methylprednisolone (MP) resulted in the dysfunction of neural stem cells (NSCs), which may help to explain the controversial roles of MP in SCI. However, the detailed mechanism is still unclear. In this manuscript, we investigated the LncRNA and mRNA expression profiles of NSCs treated with MP. A total of 63 differentially expressed LncRNAs and 174 differentially expressed mRNAs were identified. Gene ontology (GO) analysis showed that differentially expressed mRNAs were highly associated with terms related to regulation of external stimulation, secretion, and migration. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis results indicated that the PI3K–Akt signaling pathway contributed to the functions of MP treated NSCs. Besides, 3899 co-expression pairs were constructed among the differentially expressed LncRNA and mRNA, among which five predicted target mRNAs with the differentially expressed LncRNAs were identified. These results provide greater insight into the precise mechanisms of MP mediating NSC dysfunction in SCI.

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