scholarly journals Gene Expression Profile of Patients with Mayer-Rokitansky-Küster-Hauser Syndrome: New Insights into the Potential Role of Developmental Pathways

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e91010 ◽  
Author(s):  
Cristina Nodale ◽  
Simona Ceccarelli ◽  
Mariateresa Giuliano ◽  
Marcella Cammarota ◽  
Sirio D’Amici ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Potential Role ◽  
Developmental Pathways

scholarly journals The role of Alk-1 and Alk-5 in the mechanosensing of chondrocytes

2014 ◽  
Vol 19 (4) ◽  
Author(s):  
Patricia Sanz-Ramos ◽  
Javier Dotor ◽  
Iñigo Izal-Azcárate
Keyword(s):  
Gene Expression ◽  
Mouse Model ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Peptide Inhibitors ◽  
Mechanical Environment ◽  
Chondrocyte Phenotype ◽  
Cartilage Cells ◽  
Alk 5

AbstractWe aim to demonstrate the role of Alk receptors in the response of hydrogel expansion. Chondrocytes from rat knees were cultured onto plastic and hydrogel surfaces. Alk-1 and Alk-5 were overexpressed or silenced and the effects on cells during expansion were tested and confirmed using peptide inhibitors for TGFβ. Overexpression of Alk-5 and silencing of Alk-1 led to a loss of the chondrocyte phenotype, proving that they are key regulators of chondrocyte mechanosensing. An analysis of the gene expression profile during the expansion of these modified cartilage cells in plastic showed a better maintenance of the chondrocyte phenotype, at least during the first passages. These passages were also assayed in a mouse model of intramuscular chondrogenesis. Our findings indicate that these two receptors are important mediators in the response of chondrocytes to changes in the mechanical environment, making them suitable targets for modulating chondrogenesis. Inhibition of TGFβ could also be effective in improving chondrocyte activity in aged or expanded cells that overexpress Alk-1.

Cell death gene expression profile: Role of RIPK2 in dengue virus-mediated apoptosis

2011 ◽  
Vol 156 (1-2) ◽  
pp. 25-34 ◽  
Author(s):  
Atthapan Morchang ◽  
Umpa Yasamut ◽  
Janjuree Netsawang ◽  
Sansanee Noisakran ◽  
Wiyada Wongwiwat ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Death ◽  
Dengue Virus ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Cell Death Gene

scholarly journals PB2193 GENE EXPRESSION PROFILE IN PRIMARY MYELOFIBROSIS: ROLE OF ASXL1

HemaSphere ◽  
2019 ◽  
Vol 3 (S1) ◽  
pp. 983-984
Author(s):  
S. Sánchez Sosa ◽  
C. Bilbao Sieyro ◽  
Y. Florido Ortega ◽  
E. González Pérez ◽  
M. D. L. N. Saez Perdomo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Primary Myelofibrosis

Gene Expression Profile of Protein Kinases Reveals a Distinctive Signature of Chronic Lymphocytic Leukemia (CLL) and Points to a Role of Second Generation Protein Kinase Inhibitors.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 2794-2794
Author(s):  
Simona Tavolaro ◽  
Sabina Chiaretti ◽  
Monica Messina ◽  
Francesca R. Mauro ◽  
Ilaria Del Giudice ◽  
...  
Keyword(s):  
Gene Expression ◽  
Prognostic Factors ◽  
Protein Kinase ◽  
Chronic Lymphocytic Leukemia ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Kinase Activity ◽  
Lymphocytic Leukemia ◽  
P Value

Abstract Background. Chronic lymphocytic leukemia (CLL) is characterized by the accumulation of relatively mature B cells and by a very variable clinical course. This clinical heterogeneity is sustained by different biologic parameters, such as the mutational status of the immunoglobulin variable genes (IgVH), CD38 and ZAP-70 expression. In order to investigate the potential role of protein kinase (PK) inhibitors in CLL, we evaluated the gene expression profile of 1324 probesets annotated as PK using the HGU133 Plus2.0 Affymetrix arrays. Methods. We evaluated 44 CLL and 137 acute lymphocytic leukemia (ALL) patients. Two additional sets of CLL (49 cases) were utilized to validate the results obtained. Probesets identified as PK genes were used for all the analyses, namely unsupervised clustering, Analysis of Variance (ANOVA) and t-test analysis. ANOVA was performed using a p-value of ≤0.001: further selection was performed retaining only those probesets whose mean expression level was ≥300 in at least one group and showed a fold change difference of ≥1.5 across all groups. Finally, to specifically identify genes differentially expressed between different subclasses of CLL, a t-test was applied: probesets were required to have a p-value ≤0.05 and a fold change>1.5. Results. Unsupervised analysis, performed on CLL samples and different ALL subgroups, highlighted in CLL a unique and very homogeneous pattern characterized by the overexpression of a large set of PK; these results were further confirmed by ANOVA. Moreover, we identified 16 PK genes that were highly expressed in all 3 CLL sets analyzed. These genes codify for proteins with tyrosine kinase activity (SYK, LYN, BLK, LCK, JAK1, CSK and FGR), serin-threonin kinase activity (PIM2, PFTK1, TLK1, MAP4K1, PDPK1, PRKCB1 and STK10) or both (GRK6 and WEE1). Some of the selected genes are members of important protein kinase families, involved in cellular signaling, such as Src kinases (SFK), MAPK and JAK kinase family. PK expression was also analyzed in different CLL subclasses, subdivided according to different prognostic factors; in particular, we compared IgVH mutated vs unmutated patients, CD38+ vs CD38- cases and, finally, ZAP-70+ vs ZAP-70- patients in the 3 experimental CLL sets. Comparison between IgVH mutated vs unmutated cases highlighted a differential expression of ZAP-70 in all the 3 sets analyzed. Contrariwise, no PK was associated with the other prognostic parameters. Thus, these analyses did not show a specific signature associated with the abovementioned biologic features, suggesting that PK overexpression is specific of the disease itself rather than of CLL subclasses. Conclusions. Our results show that CLL is characterized by a very peculiar PK signature and identify some potential molecular targets. Moreover, our findings indicate that a common mechanism of PK-mediated deregulation is operational in CLL cells, independently of other prognostic factors. Based on these pre-clinical data, we propose that second generation PK inhibitors may have a role in the management of all CLL patients.

scholarly journals Doxorubicin and NRG-1/erbB4-Deficiency Affect Gene Expression Profile: Involving Protein Homeostasis in Mouse

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Cecilia Vasti ◽  
Henning Witt ◽  
Matilde Said ◽  
Patricia Sorroche ◽  
Hernán García-Rivello ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Ventricular Remodeling ◽  
Current Knowledge ◽  
Ubiquitin Proteasome System ◽  
Neuregulin 1 ◽  
Hypertrophic Response ◽  
Ubiquitin Proteasome

The accumulating evidence demonstrates the essential role of neuregulin-1 signaling in the adult heart, and, moreover, indicates that an impaired neuregulin signaling exacerbates the doxorubicin-mediated cardiac toxicity. Despite this strong data, the specific cardiomyocyte targets of the active erbB2/erbB4 heterodimer remain unknown. In this paper, we examined pathways involved in cardiomyocyte damage as a result of the cardiac sensitization to anthracycline toxicity in the ventricular muscle-specific erbB4 knockout mouse. We performed morphological analyses to evaluate the ventricular remodeling and employed a cDNA microarray to assess the characteristic gene expression profile, verified data by real-time RT-PCR, and then grouped into functional categories and pathways. We confirm the upregulation of genes related to the classical signature of a hypertrophic response, implicating an erbB2-dependent mechanism in doxorubicin-treated erbB4-KO hearts. Our results indicate the remarkable downregulation of IGF-I/PI-3′ kinase pathway and extends our current knowledge by uncovering an altered ubiquitin-proteasome system leading to cardiomyocyte autophagic vacuolization.

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