Indigofera oblongifolia Prevents Lead Acetate-Induced Hepatotoxicity, Oxidative Stress, Fibrosis and Apoptosis in Rats

The effects of acute lead exposure on renal function, lipid peroxidation and the expression of haeme oxygenase (HO) in rat kidney were determined. A single injection of lead acetate (50 mg Pb/kg) was given to rats. Changes in renal function, characterized by a significant reduction in the Na excretion was observed six hours after Pb exposure; this effect persisted for 24 hours. TBARS levels increased in kidney cortex 24 hours after Pb administration. In kidney cortex, Pb exposure affected the expression of HO-1, a renal protein associated with oxidative stress. HO-1 mRNA increased 2.3-fold, three hours after Pb administration and remained increased for six, 12 and 24 hours. HO enzymatic activity and HO-1 protein increased six and three hours after Pb administration, respectively, and remained increased at 24 hours. HO inhibition by tin-protoporphyrin, potentiated Pb-induced increase in TBARS and prevented the Pb-induced reduction in Na excretion. Our data suggest that Pb may be acting through the generation of oxidant products and induction of HO.

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Protective Effect of Alpha-lipoic Acid Against Lead Acetate-Induced Oxidative Stress in the Bone Marrow of Rats

International Journal of Pharmacology ◽

10.3923/ijp.2011.217.227 ◽

2011 ◽

Vol 7 (2) ◽

pp. 217-227 ◽

Cited By ~ 6

Author(s):

Nagaraja Haleagraha ◽

Tan Jackie ◽

Srikumar Chakravart ◽

Anupama Bangra Kulur

Keyword(s):

Oxidative Stress ◽

Bone Marrow ◽

Protective Effect ◽

Lipoic Acid ◽

Lead Acetate ◽

Alpha Lipoic Acid

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Protective Effects of Salidroside on Lead Acetate-induced Oxidative Stress and Hepatotoxicity in Sprague-Dawley Rats

Biological Trace Element Research ◽

10.1007/s12011-019-1635-8 ◽

2019 ◽

Vol 191 (2) ◽

pp. 426-434 ◽

Cited By ~ 6

Author(s):

Chen Chen ◽

Beibei Lin ◽

Shanshan Qi ◽

Jia He ◽

Hongxing Zheng

Keyword(s):

Oxidative Stress ◽

Lead Acetate ◽

Protective Effects ◽

Sprague Dawley ◽

Sprague Dawley Rats

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Curcumin Attenuates Lead-Induced Cerebellar Toxicity in Rats via Chelating Activity and Inhibition of Oxidative Stress

Biomolecules ◽

10.3390/biom9090453 ◽

2019 ◽

Vol 9 (9) ◽

pp. 453 ◽

Cited By ~ 5

Author(s):

Kabeer Abubakar ◽

Maryam Muhammad Mailafiya ◽

Abubakar Danmaigoro ◽

Samaila Musa Chiroma ◽

Ezamin Bin Abdul Rahim ◽

...

Keyword(s):

Oxidative Stress ◽

Treatment Group ◽

Lead Acetate ◽

Curcuma Longa ◽

Oral Treatment ◽

Active Constituent ◽

Sod Activity ◽

Chelating Activity ◽

Histological Architecture ◽

Pb Concentration

Lead (Pb) is a toxic, environmental heavy metal that induces serious clinical defects in all organs, with the nervous system being its primary target. Curcumin is the main active constituent of turmeric rhizome (Curcuma longa) with strong antioxidant and anti-inflammatory properties. This study is aimed at evaluating the therapeutic potentials of curcumin on Pb-induced neurotoxicity. Thirty-six male Sprague Dawley rats were randomly assigned into five groups with 12 rats in the control (normal saline) and 6 rats in each of groups, i.e., the lead-treated group (LTG) (50 mg/kg lead acetate for four weeks), recovery group (RC) (50 mg/kg lead acetate for four weeks), treatment group 1 (Cur100) (50 mg/kg lead acetate for four weeks, followed by 100 mg/kg curcumin for four weeks) and treatment group 2 (Cur200) (50 mg/kg lead acetate for four weeks, followed by 200 mg/kg curcumin for four weeks). All experimental groups received oral treatment via orogastric tube on alternate days. Motor function was assessed using a horizontal bar method. The cerebellar concentration of Pb was evaluated using ICP-MS technique. Pb-administered rats showed a significant decrease in motor scores and Superoxide Dismutase (SOD) activity with increased Malondialdehyde (MDA) levels. In addition, a marked increase in cerebellar Pb concentration and alterations in the histological architecture of the cerebellar cortex layers were recorded. However, treatment with curcumin improved the motor score, reduced Pb concentration in the cerebellum, and ameliorated the markers of oxidative stress, as well as restored the histological architecture of the cerebellum. The results of this study suggest that curcumin attenuates Pb-induced neurotoxicity via inhibition of oxidative stress and chelating activity.

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Honey prevents neurobehavioural deficit and oxidative stress induced by lead acetate exposure in male wistar rats- a preliminary study

Metabolic Brain Disease ◽

10.1007/s11011-015-9733-6 ◽

2015 ◽

Vol 31 (1) ◽

pp. 37-44 ◽

Cited By ~ 19

Author(s):

Wahab Imam Abdulmajeed ◽

Habeeb Bolakale Sulieman ◽

Maymunah Oloruntosin Zubayr ◽

Aminu Imam ◽

Abdulbasit Amin ◽

...

Keyword(s):

Oxidative Stress ◽

Lead Acetate ◽

Wistar Rats ◽

Male Wistar Rats ◽

Preliminary Study ◽

And Oxidative Stress

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Indigofera oblongifolia Ameliorates Lead Acetate-Induced Testicular Oxidative Damage and Apoptosis in a Rat Model

Biological Trace Element Research ◽

10.1007/s12011-016-0689-0 ◽

2016 ◽

Vol 173 (2) ◽

pp. 354-361 ◽

Cited By ~ 21

Author(s):

Mohamed A. Dkhil ◽

Ahmed E. Abdel Moneim ◽

Saleh Al-Quraishy

Keyword(s):

Oxidative Damage ◽

Rat Model ◽

Lead Acetate ◽

Indigofera Oblongifolia

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Berberis vulgaris L. effects on oxidative stress and liver injury in lead-intoxicated mice

Journal of Complementary and Integrative Medicine ◽

10.1515/jcim-2015-0079 ◽

2017 ◽

Vol 14 (1) ◽

Cited By ~ 10

Author(s):

Jawhar Laamech ◽

Jaouad El-Hilaly ◽

Hamadi Fetoui ◽

Yassine Chtourou ◽

Hanane Gouitaa ◽

...

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Aqueous Extract ◽

Lead Acetate ◽

Protein Carbonyls ◽

Control Group ◽

Distilled Water ◽

Once Daily ◽

Group 2 ◽

Mice Liver

AbstractBackgroundL. (BV), commonly known as “MethodsSixty IOPS mice were divided into six groups and were treated as follows: group 1 (normal control) received double distilled water; group 2 (toxic control) received lead acetate (5 mg/kg body weight/day) in double distilled water for 40 days; groups 3–6 received BV aqueous extract at doses of 25, 50, 100 and 150 mg/kg body weight , respectively, once daily for 30 days from 11 day after beginning of lead acetate exposure to the end of the experiment.ResultsToxic control group showed a significant alteration of serum alanine-aminotransferase (ALT), aspartate-aminotransferase (AST), total cholesterol (TC), total bilirubin (TB), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and reduced glutathione (GSH). Histological assessment of lead-intoxicated mice liver revealed alterations in hepatocytes and focal necrosis. BV treatment significantly prevented lead accumulation, increased ALT, AST, TC, and TB, inhibited lipid peroxidation and protein carbonyls(PCO) formation. Additionally, BV extract normalized the antioxidant enzymes (CAT, SOD and GPx), GSH and architecture of liver tissues.ConclusionsBV aqueous extract exerts significant hepatoprotective effects against lead-induced oxidative stress and liver dysfunction. The BV effect may be mediated through the enhancement of antioxidant status, lead-chelating abilities and free radicals quenching.

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