scholarly journals Simultaneous study of antioxidant activity, DNA protection and anti-inflammatory effect of Vernonia amygdalina leaves extracts

PLoS ONE ◽  
2020 ◽  
Vol 15 (7) ◽  
pp. e0235717
Author(s):  
Wei-Te Wang ◽  
Su-Fen Liao ◽  
Zih-Ling Wu ◽  
Chia-Wei Chang ◽  
Jane-Yii Wu
Keyword(s):  
Antioxidant Activity ◽  
Vernonia Amygdalina ◽  
Dna Protection ◽  
Simultaneous Study ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals In vitro antioxidant, anti-inflammatory and anti-cancer activities of methanolic extract of Asparagus horridus grows in North Cyprus Kuzey Kıbrıs da yetişen Asparagus horridus metanolik ekstraktının in-vitro antioksidan, anti-enflamatuar ve anti-kanser aktivitesi

2020 ◽  
Vol 45 (4) ◽  
pp. 365-372
Author(s):  
Ergul Mutlu Altundag ◽  
Duygu Gençalp ◽  
Cahit Özbilenler ◽  
Kübra Toprak ◽  
Namık Kerküklü
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Protein Denaturation ◽  
Soxhlet Extraction ◽  
Edible Plant ◽  
North Cyprus ◽  
Anti Cancer ◽  
Anti Inflammatory ◽  
Inflammatory Effect

AbstractBackgroundAsparagus horridus is an edible plant known as “Ayrelli” in North Cyprus. The scientific literature has not yet submitted a report about the antioxidant, anti-inflammatory and anti-cancer activities of A. horridus plant from North Cyprus until now. The purpose of the research was to determine the antioxidant, anti-inflammatory and anti-cancer activities of A. horridus.Materials and methodsSoxhlet extraction of A. horridus was performed using methanol. Antioxidant activity was determined by DPPH, TFC, FRAP and TPC assays. Protein-denaturation assay was performed to determine the anti-inflammatory effect. The anti-cancer effects of the extract on HepG2 and B-CPAP cell lines were determined with MTT assay.ResultsAntioxidant activity for A. horridus extract was determined by DPPH (50%), TFC (266.26 μg QUE/mg extract), FRAP (1.27 μg FeSO4/mg extract) and TPC (167.613 μg GAE/mg extract) assays at 25 mg/mL. Inhibition of protein-denaturation activity was found as 29.42% at 25 mg/mL. After 24 h of the extract treatment, cell proliferation of HepG2 and B-CPAP cancer cells were inhibited at IC50 values 63.24 μg/mL and 101.24 μg/mL, respectively.ConclusionThese results have shown that the methanol extract of A. horridus grows in North Cyprus has antioxidant, anti-inflammatory and anti-cancer activities.

scholarly journals Phenolic Content, Antioxidant Activity, Anti-Inflammatory Potential, and Acute Toxicity Study of Thymus leptobotrys Murb. Extracts

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Asmaa Oubihi ◽  
Hanae Hosni ◽  
Issmail Nounah ◽  
Abdessamad Ettouil ◽  
Hicham Harhar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Female Rats ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Control Group ◽  
Standard Drug ◽  
Methanolic Extracts ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Thymus leptobotrys is a medicinal plant belonging to the Lamiaceae family, endemic in Morocco, and used in traditional medicine. The present work aims to study the phenolic compounds, the antioxidant activity, the anti-inflammatory effect, and the toxicity of two ethanolic and methanolic extracts of Thymus leptobotrys aerial part. The yield of the methanolic extraction (22.2%) is higher than that of the ethanolic extraction (15.8%) and is characterized by higher contents of polyphenols 243.08 mg/g GAE (mg/g of gallic acid), flavonoids 179.28 mg/g RE (mg/g of rutin), and tannins 39.31 mg/g CE (mg/g of catechin). The in vitro measurement of antioxidant activity with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical reduction test and Trolox equivalent antioxidant capacity (TEAC) test demonstrates the higher performance of the methanolic extract. The evaluation of the anti-inflammatory effect in vivo on adult Wistar female rats leads to a very significant decrease in the inflammation of the edema compared to the standard drug (indomethacin) and the control group. The toxicity test reveals that both extracts showed no toxicity within an LD50 above 2000 mg/kg body weight of the rats.

scholarly journals Antioxidant and anti-inflammatory effects of polyphenols extracted from Ilex latifolia Thunb

RSC Advances ◽  
2018 ◽  
Vol 8 (13) ◽  
pp. 7134-7141 ◽  
Author(s):  
Tian-Tian Zhang ◽  
Ting Hu ◽  
Jian-Guo Jiang ◽  
Jing-Wen Zhao ◽  
Wei Zhu
Keyword(s):  
Antioxidant Activity ◽  
Tea Polyphenols ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Mouse Macrophage ◽  
Cellular Antioxidant Activity ◽  
Anti Inflammatory ◽  
Inflammatory Effect

To promote the application of Ilex latifolia Thunb., a Chinese bitter tea, polyphenols were extracted from its leaves and their cellular antioxidant activity (CAA) and anti-inflammatory effect against mouse macrophage RAW 264.7 cells were analyzed.

In vivo photoprotective and anti-inflammatory effect of hyperforin is associated with high antioxidant activity in vitro and ex vivo

2012 ◽  
Vol 81 (2) ◽  
pp. 346-350 ◽  
Author(s):  
Martina C. Meinke ◽  
Sabine Schanzer ◽  
Stefan F. Haag ◽  
Federica Casetti ◽  
Marcel L. Müller ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ex Vivo ◽  
High Antioxidant Activity ◽  
Anti Inflammatory ◽  
Inflammatory Effect

CHEMICAL INDICES, ANTIOXIDANT ACTIVITY AND ANTI-INFLAMMATORY EFFECT OF EXTRACTS OF THE MEDICINAL RICE “NJAVARA” AND STAPLE VARIETIES: A COMPARATIVE STUDY

2012 ◽  
Vol 37 (3) ◽  
pp. 369-380 ◽  
Author(s):  
SMITHA MOHANLAL ◽  
RATHNAM PARVATHY ◽  
VASANTHA SHALINI ◽  
RATHEESH MOHANAN ◽  
ANTONY HELEN ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Comparative Study ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals An Experimental Exploratory Study for the Mechanism of Anti-Inflammatory Action of Mecca Myrrh (Commiphora opobalsamum)

2021 ◽  
pp. 152-165
Author(s):  
Akram A. Al–Salmi ◽  
Mai A. Alim A. Sattar Ahmad ◽  
Lateef M. Khan
Keyword(s):  
Antioxidant Activity ◽  
Inhibitory Effect ◽  
Significant Inhibition ◽  
Control Group ◽  
Edema Formation ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
And Control ◽  
Inflammatory Action ◽  
Inflammatory Effect

Background: Our recent past studies accomplished the target to investigate the anti-inflammatory effect as well as toxicological profile of Commiphora opobalsamum (CO), with almost identical potency in comparison to the contemporary anti-inflammatory drugs. This inspired us to explore its mechanism of action to further strengthen its efficacy. Aim: To investigate the mechanism of anti-inflammatory action of CO by exploration of its correlation to its antioxidant activity as well as inhibitory effect on inflammatory mediators by interaction with MDA, NO, PGE2 and TNF-α. Methods: 10 weeks old male Swiss albino mice (30 to 40 g) were used. Carrageenan–induced paw edema method was used, pretreatment with CO alone in different doses and in combination with diclofenac was done prior to carrageenan administration, subsequently homogenate of the paw was used to quantify the levels of MDA, NO, PGE2 and TNF-α by using their specific assays. In addition, Histological examination of edema paw was performed to evaluate the anti-inflammatory effect of CO extract versus diclofenac and control investigating their impact on the inflammatory cell migration and edema formation. Results: CO extract in the dose of 500mg/kg demonstrated maximum reduction of MDA level; hence antioxidant activity of CO could be contributed to its anti-inflammatory effect. Furthermore, notable observation indicated that CO administration significantly suppress increment in NO level in response to carrageenan, unequivocally significant inhibition of PGE2 accumulation at the site of inflammation by the pretreatment of CO extract was observed in the dose of 500mg/kg (0.001). Finally in contrast, the CO extract in the dose of 500mg/kg significantly accomplished the reduction of TNF-α in the paw in comparison to the control group (p<0.05). Conclusion: In this study we have illustrated a pioneering perspective to elucidate the mechanism of anti- inflammatory and antioxidant action of methanolic extract of CO, attributed through suppression of MDA. NO, PGE2 and TNF-α at the site of inflammation.

scholarly journals The Development of Innovative Clay Mask Product Mixed with Theacanthus ebracteatus Vahl Extract

2021 ◽  
pp. 364-370
Author(s):  
Narin Kakatum ◽  
Papawee Sookdee ◽  
Thanaporn Phromhan ◽  
Ilham Hamai ◽  
Natphapat Supatsaraphokin
Keyword(s):  
Antioxidant Activity ◽  
Skin Irritation ◽  
Assay Method ◽  
Total Phenolic ◽  
Activity Test ◽  
Irritation Test ◽  
Acanthus Ebracteatus ◽  
Anti Inflammatory ◽  
Effect Test ◽  
Inflammatory Effect

This research is a study on the development of innovative clay mask mixed with the Acanthus ebracteatus Vahl extract product. The purposes of this research are as follows. Firstly, the research is to develop a clay mask product mixed with natural extracts from the Acanthus ebracteatus Vahl. Secondly, it is aimed to study on the antioxidant activity, anti-inflammatory effect, antimicrobial effect and total Phenolic contents of the Acanthus ebracteatus Vahl extracts. Thirdly, it is tostudy stability of the product. Finally, the research is to study on the calculation of suitable product formulations for clay products mixed with Acanthus ebracteatus Vahl extracts. By starting from the selection of raw materials, the extracts are prepared to determine the total phenolic content. Later, 2,2-diphenyl-1-picrylhydrazyl (DPPH) Assay method is conducted for antioxidant activity test, followed by anti-inflammatory effect test (Nitric oxide production inhibitory activity test, inhibition of LPS-induced TNF-α secretion test and Anti-inflammatory activity test (COX-2). Afterwards, a suitable formulation is developed. The test for the safety and physical properties of the product is then carried out followed by the skin irritation test in a group of 10 volunteers in order to ensure that the clay mask product containing Acanthus ebracteatus Vahl extracts is effective and safe. From the results of the product stability study, the findings indicated that the clay mask product containing Acanthus ebracteatus Vahl extracts did not show any phase separation. The irritation test in 10 volunteers after 12 hours of using the product showed no sign of allergic reaction, itching or irritation. Toxicity test results found no cytotoxicity. The anti-inflammatory effect test was found to have potent anti-inflammatory properties, when tested in the RAW 264.7 macrophage cell. This study is only for the development of an innovative product of skin clay mask mixed with the Acanthus ebracteatus Vahl extracts.

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