scholarly journals Genetic and morphometric variability between populations of Betula ×oycoviensis from Poland and Czechia: A revised view of the taxonomic treatment of the Ojców birch

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243310
Author(s):  
Rostislav Linda ◽  
Ivan Kuneš ◽  
Martin Baláš
Keyword(s):  
Genetic Variability ◽  
Genome Size ◽  
Parental Species ◽  
Morphological Variability ◽  
Taxonomic Position ◽  
Polish Population ◽  
Separate Species ◽  
Taxonomic Treatment ◽  
Czech Population

Birches are generally known for their high genetic and morphological variability, which has resulted in the description of many species. Ojców birch was described in 1809 by Willibald Suibert Joseph Gottlieb Besser in Poland. Since then, several studies assessing its taxonomy were conducted. Today, various authors present Ojców birch at different taxonomic ranks. In Czechia, the Ojców birch is classified a critically endangered taxon and confirmed at one locality consisting of several tens of individuals. However, before a strategy for its conservation can be applied, we consider it necessary to assess the taxonomic position of the endangered Czech population and to evaluate its relationship to the original Polish population. This study aimed to evaluate the morphometric and genetic variability between populations of B. ×oycoviensis in Poland and the Czechia and their relationship to regional populations of B. pendula, one of the putative parental species of the Ojców birch. Altogether, 106 individuals were sampled, including the holotype of B. szaferi, the second putative parental species of B. ×oycoviensis, received from the herbarium of W. Szafer, which is deposited at the Institute of Botany in Kraków. Morphological analyses identified differences in leaves between B. ×oycoviensis and B. pendula. However, no significant differences were found in genome size between selected taxa/working units except for B. pendula sampled in Czechia. The identified difference of the Czech population of B. pendula is probably caused by population variability. Genetic variability between all the taxa under comparison, regardless of their origin, was also very low; only the benchmark taxa (B. nana and B. humilis) clearly differed from all samples analyzed. The results indicate minute morphological and negligible genetic variability between the Czech and Polish populations of B. ×oycoviensis. In light of our results, the classification of B. ×oycoviensis as B. pendula var. oycoviensis seems more accurate than all hitherto presented alternatives (e.g. B. ×oycoviensis as a separate species).

Overlooked morphological variation and a proposal for a new taxonomic circumscription of Alyssum simplex (Brassicaceae)

Phytotaxa ◽  
2019 ◽  
Vol 416 (2) ◽  
pp. 149-166
Author(s):  
VERONIKA CETLOVÁ ◽  
JAVIER FUERTES-AGUILAR ◽  
DARIA IUDOVA ◽  
STANISLAV ŠPANIEL
Keyword(s):  
Genome Size ◽  
Genetic Markers ◽  
Geographic Distribution ◽  
Morphological Variation ◽  
Related Species ◽  
Evolutionary History ◽  
Separate Species ◽  
Taxonomic Treatment ◽  
Morphological Differences

A recent study of European annual taxa of Alyssum has revealed that A. simplex includes two cytotypes (diploid and tetraploid) which differ in genetic markers, evolutionary history, and monoploid relative genome size. In this paper we present a new taxonomic treatment of A. simplex in which we treat the two cytotypes as two separate species. We select and discuss suitable names for both taxa (diploid A. simplex and tetraploid A. collinum), examine their morphological differences and survey their geographic distribution. In addition, we compare both cytotypes with the morphologically similar and genetically related species A. strigosum and designate lectotypes and a neotype of several relevant names.

Morphological and genetic variability of Baetis (Rhodobaetis) braaschi Zimmermann, 1980 (Ephemeroptera: Baetidae)

Zootaxa ◽  
2012 ◽  
Vol 3323 (1) ◽  
pp. 27 ◽  
Author(s):  
PAVEL SROKA ◽  
ALEXANDER V. MARTYNOV ◽  
ROMAN J. GODUNKO
Keyword(s):  
Genetic Variability ◽  
Methodological Approach ◽  
Morphological Variability ◽  
Geographic Origin ◽  
Intraspecific Variability ◽  
Morphological Data ◽  
Mtdna Coi ◽  
Geographic Regions ◽  
Coi Sequences ◽  
The Individual

Specimens of Baetis (Rhodobaetis) braaschi Zimmermann, 1980 from the three distant geographic regions (Crimean Pen-insula, Eastern Ukraine and Caucasus) are investigated and compared using a methodological approach combining mor-phological and molecular (partial mtDNA COI sequences) data. Intraspecific variability in several morphologicalcharacters is recognized and described, whereas COI sequences are found to be very uniform. The amount and distributionof the changes of COI sequences do not follow the pattern of morphological variability and/or geographic origin of thespecimens. This indicates that analysis of the changes in the COI sequence can contradict the pattern of morphologicalcharacters commonly used for the discrimination of the individual Rhodobaetis species. As a basis for the future taxonom-ic changes concerning subgenus Rhodobaetis, it is advised (where possible) to critically evaluate both molecular and morphological data.

scholarly journals GENOME SIZE IN THREE SPECIES OF Glandularia AND THEIR HYBRIDS

2019 ◽  
Vol 30 (2) ◽  
pp. 47-54
Author(s):  
M.R. Ferrari ◽  
E.J. Greizerstein ◽  
L. Poggio
Keyword(s):  
Genome Size ◽  
Dna Content ◽  
High Frequency ◽  
Parental Species ◽  
The Other ◽  
F1 Hybrids ◽  
Homoeologous Pairing ◽  
The Relationship ◽  
Species Being ◽  
Homoeologous Chromosomes

In this work the relationship between genome size of Glandularia species and the meiotic configurations found in their hybrids are discussed. Glandularia incisa (Hook.) Tronc., growing in two localities of Corrientes and Córdoba provinces, Argentina, with different ecological conditions, showed inter-population variability of the 2C-value. The DNA content found in the Corrientes locality (2.41 pg) was higher than that obtained in the Córdoba locality (2.09 pg) which has more stressful environmental conditions than the former. These values are statistically different from those that were found in Glandularia pulchella (Sweet) Tronc. from Corrientes (1.43 pg) and in Glandularia perakii Cov. et Schn from Córdoba (1.47 pg). The DNA content of the diploid F1 hybrids, G. pulchella × G. incisa and G. perakii × G. incisa, differed statistically from the DNA content of the parental species, being intermediate between them. Differences in the frequency of pairing of homoeologous chromosomes were observed in the hybrids; these differences cannot be explained by differences in genome size since hybrids with similar DNA content differ significantly in their meiotic behavior. On the other hand, the differences in the DNA content between the parental species justify the presence of a high frequency of heteromorphic open and closed bivalents and univalents with different size in the hybrids. Key words: Intra-specific DNA content variability, homoeologous pairing, heteromorphic bivalents

scholarly journals Identification of European and Japanese Larch and Their Interspecific Hybrid with Morphological Markers: Application to Young Seedlings

2006 ◽  
Vol 55 (1-6) ◽  
pp. 123-134 ◽  
Author(s):  
L. E. Pâques ◽  
G. Philippe ◽  
D. Prat
Keyword(s):  
Molecular Markers ◽  
Parental Species ◽  
Correct Classification ◽  
Morphological Markers ◽  
Hybrid Features ◽  
Pure Species ◽  
Seed Orchards ◽  
Linear Discriminant ◽  
Non Destructive

Abstract Open-pollinated hybridisation seed orchards of European and Japanese larches produce mixed progenies combining a highly variable proportion of hybrids along with pure parental species. For several reasons, it is desirable to identify and to sort out hybrids from pure species at the seedling stage. Taxa identification of 1-2 yr-old seedlings was attempted using non-destructive assessment of several traits, including morphology, phenology, growth and architecture parameters. Two sets of progenies originating from 10 open-pollinated hybridisation seed orchards were used, relying in a first step on taxa identification of individual seedlings with diagnostic molecular markers. Based on 21 traits assessed, some clear trends in pure species and hybrid features were apparent but due to the large and overlapping ranges of taxa characteristics, no single parameter allowed unambiguous identification of taxa. Combination of traits through linear discriminant analysis made possible correct classification of 90.2% to 98.6% of individuals depending on the orchard although there were a few problematic orchards. Two traits appeared particularly pertinent for discriminating young plants taxa, namely 1st-yr leaf retention (marcescence) and the bark colour of 2nd-year shoot increments. Results were corroborated using progenies from several orchards and over two experimental periods.

New varieties of Diphasiastrum zeilleri (Rouy) Holub (Lycopodiaceae)

2016 ◽  
pp. 5-21
Author(s):  
Yu. A. Ivanenko
Keyword(s):  
Parental Species ◽  
Introgressive Hybridization ◽  
Growing Season ◽  
High Variability ◽  
Separate Species ◽  
New Variety ◽  
Hybrid Species ◽  
New Varieties ◽  
Light And Shadow ◽  
Ecological Forms

Diphasiastrum zeilleri (Rouy) Holub is a hybrid species, with the formula D. complanatum (L.) Holub × D. tristachyum (Pursh) Holub. A high variability of D. zeilleri is due to environmental conditions (light and shadow ecological forms) and probably to introgressive hybridization with the parental species. Intermediate forms between D. zeilleri, D. complanatum and D. tristachyum are quite rare, that allows to treat the three taxa as separate species. D. zeilleri is missing in subarctic regions of Europe and North America, and some degree of its resemblance with D. complanatum subsp. montellii Kukk. is convergent. Lycopodium complanatum f. polystachyum H. Lindb. is considered to belong to D. zeilleri, and an appropriate conbination is published, Diphasiastrum zeilleri var. polystachyum (H. Lindb.) Ivanenko. A new variety Diphasiastrum zeilleri (Rouy) Holub var. ramistachyum Ivanenko is described, differing by sessile strobili formed on the basal part of fertile branches during one growing season. The structure, the base of which is a fertile branch, and the upper part is strobilus (strobili), is proposed to term «strobilus branch». Besides D. zeilleri var. ramistachyum, the strobilus branches are produced by D. issleri (Rouy) Holub, D. oellgaardii Stoor, Boudrie, Jérôme, K. Horn et Bennert, and rarely by D. alpinum (L.) Holub and D. complanatum.

scholarly journals Relationships among Morphological Characters, Isozymes Polymorphism and DNA Variability – the Impact on Lactuca Germplasm Taxonomy

2011 ◽  
Vol 39 (No. 2) ◽  
pp. 59-67 ◽  
Author(s):  
I. Doležalová ◽  
A. Lebeda ◽  
M. Dziechciarková ◽  
E. Křístková ◽  
D. Astley ◽  
...  
Keyword(s):  
Dna Content ◽  
Fragment Length Polymorphism ◽  
Morphological Variability ◽  
Morphological Characters ◽  
Aflp Analysis ◽  
Dapi Staining ◽  
Lactuca Species ◽  
Relative Dna Content ◽  
The Impact

Fifty one accessions of nineteen Lactuca species, the hybrid L. serriola × L. sativa and the related species Mycelis muralis were evaluated for morphological variability, esterase (EST) polymorphism, Amplified Fragment Length Polymorphism (AFLP) and relative DNA content. Sixteen Lactuca accessions were classified taxonomically on the basis of morphology, isozyme analysis and AFLP. Twenty-eight bands (isoforms) of EST were recorded allowing 82% of accessions to be distinguished. The relative DNA content, measured using flow-cytometry (DAPI staining), ranged from 2.02 pg in L. capensis to 17.96 pg in L. canadensis. The results from AFLP analysis and the relative DNA content measurement corresponded well with recent taxonomic classification of the genus Lactuca.  

scholarly journals Reciprocal hybridization between diploid Ficaria calthifolia and tetraploid Ficaria verna subsp. verna: evidence from experimental crossing, genome size and molecular markers

2019 ◽  
Vol 189 (3) ◽  
pp. 293-310 ◽  
Author(s):  
Ondřej Popelka ◽  
Michal Sochor ◽  
Martin Duchoslav
Keyword(s):  
Genome Size ◽  
Morphological Variability ◽  
Plastid Dna ◽  
Genotypic Diversity ◽  
Hybrid Origin ◽  
Aflp Analysis ◽  
Sympatric Populations ◽  
Aflp Data ◽  
Evolutionary Forces ◽  
Genetic Clusters

Abstract Ficaria is a taxonomically intriguing polyploid complex with high morphological variability. Both hybridization and polyploidization have been suggested as the main evolutionary forces behind the high morphological variability in this genus; however, detailed studies are lacking. In Central Europe, two Ficaria taxa (diploid F. calthifolia and tetraploid F. verna subsp. verna) occasionally co-occur in local sympatry, which might result in hybridization. We investigated sympatric populations of the two Ficaria taxa using flow cytometry, chromosome counts, AFLP analysis and plastid DNA sequencing; we also performed experimental homoploid and heteroploid crosses to determine the frequency and direction of hybrid triploid formation, an alternative route of triploid origin (autopolyploidy) and the possibility of a one-step neoallotetraploid origin. Sympatric populations were composed of three genetic clusters corresponding to diploid F. calthifolia (2n = 16), tetraploid F. verna subsp. verna (2n = 32) and triploid plants (2n = 24). The holoploid genome size and AFLP data suggest a hybrid origin of the triploids, thereby making their formation via autopolyploidization in F. calthifolia unlikely. The triploid populations are monoclonal and of independent origin. In contrast, the parental populations exhibit high genotypic diversity and frequent sexual reproduction, including those of predominantly asexual F. verna subsp. verna. Experimental crossing confirmed that both parental taxa produce fertile seeds via a sexual pathway, but not by apomixis, and that both serve as pollen acceptors in heteroploid crosses, which is consistent with the plastid sequencing. However, hybridization is asymmetric, with maternal-excess crosses being significantly more successful. No signs of neoautotetraploidization or neoallotetraploidization were detected. In summary, recent gene flow between the studied Ficaria taxa is either limited or absent.

scholarly journals Using Color to Define Species Boundaries: Quantitative Analysis in the Orchard Oriole Complex Supports the Recognition of two Species

The Condor ◽  
2007 ◽  
Vol 109 (3) ◽  
pp. 692-697 ◽  
Author(s):  
Lynna M. Kiere ◽  
Christopher M. Hofmann ◽  
Ian E. Tracy ◽  
Thomas W. Cronin ◽  
Jeff Leips ◽  
...  
Keyword(s):  
Adult Male ◽  
Spectral Characteristics ◽  
Color Measurement ◽  
Species Boundaries ◽  
Plumage Color ◽  
Separate Species ◽  
Reflectance Spectrometry ◽  
Ideal System ◽  
Molecular Studies

Abstract Abstract. The recent divergence of Orchard (Icterus spurius spurius) and Fuertes's Orioles (I. s. fuertesi) makes them an ideal system for investigating species boundaries. Orchard and Fuertes's Orioles differ in several respects. They have distinct breeding ranges—Fuertes's Orioles breed in eastern coastal Mexico, whereas Orchard Orioles breed throughout eastern and central North America—and differ in plumage coloration, with adult male Orchard Orioles appearing “chestnut” and Fuertes's Orioles “ochre.” However, no detailed quantitative color analyses have been conducted. To characterize these differences we quantitatively measured plumage color using reflectance spectrometry. The colored plumages of adult male Orchard and Fuertes's Orioles have unique spectral characteristics, with no color overlap between them. Combined with life history differences and previous molecular studies, these findings support the classification of Orchard and Fuertes's Orioles as separate species. Additionally, this study demonstrates the utility of quantitative color measurement as a tool for examining species boundaries.

scholarly journals CLASSIFICATION OF LIFE BY THE MECHANISM OF GENOME SIZE EVOLUTION

2009 ◽  
Vol 23 (30) ◽  
pp. 3563-3580 ◽  
Author(s):  
DIRSON JIAN LI ◽  
SHENGLI ZHANG
Keyword(s):  
Genome Size ◽  
Phylogenetic Trees ◽  
Living System ◽  
Protein Length ◽  
Genome Size Evolution ◽  
Size Evolution ◽  
C Value ◽  
Stable Characteristic ◽  
Main Component

We find that the global relationships among species should be of circular phylogeny, which is quite different from common sense based on phylogenetic trees. A domain can be defined by a distinct phylogenetic circle, which is a global and stable characteristic of the living system. The mechanism in genome size evolution has been clarified; hence the main component questions on C-value enigma can be explained. We find the intrinsic relationship between genome size evolution and protein length evolution; that is the genome size and non-coding DNA ratio can be calculated based on protein length distributions.

scholarly journals Phylogeny and morphology of genus Nephrocytium (Sphaeropleales, Chlorophyceae, Chlorophyta) from China

Phytotaxa ◽  
2017 ◽  
Vol 319 (1) ◽  
pp. 84 ◽  
Author(s):  
XUDONG LIU ◽  
HUAN ZHU ◽  
BENWEN LIU ◽  
GUOXIANG LIU ◽  
ZHENGYU HU
Keyword(s):  
Phylogenetic Analyses ◽  
Light And Electron Microscopy ◽  
Taxonomic Position ◽  
Phylogenetic Position ◽  
Phytoplankton Communities ◽  
Close Phylogenetic Relationship ◽  
The Family ◽  
Close Relationship ◽  
Molecular Phylogenetic

The genus Nephrocytium Nägeli is a common member of phytoplankton communities that has a distinctive morphology. Its taxonomic position is traditionally considered to be within the family Oocystaceae (Trebouxiophyceae). However, research on its ultrastructure is rare, and the phylogenetic position has not yet been determined. In this study, two strains of Nephrocytium, N. agardhianum Nägeli and N. limneticum (G.M.Smith) G.M.Smith, were identified and successfully cultured in the laboratory. Morphological inspection by light and electron microscopy and molecular phylogenetic analyses were performed to explore the taxonomic position. Ultrastructure implied a likely irregular network of dense and fine ribs on the surface of the daughter cell wall that resembled that of the genus Chromochloris Kol & Chodat (Chromochloridaceae). Phylogenetic analyses revealed that Nephrocytium formed an independent lineage in the order Sphaeropleales (Chlorophyceae) with high support values and a close phylogenetic relationship with Chromochloris. Based on combined morphological, ultrastructural and phylogenetic data, we propose a re-classification of Nephrocytium into Sphaeropleales, sharing a close relationship with Chromochloris.

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