Phylogeny and morphology of genus Nephrocytium (Sphaeropleales, Chlorophyceae, Chlorophyta) from China

The genus Nephrocytium Nägeli is a common member of phytoplankton communities that has a distinctive morphology. Its taxonomic position is traditionally considered to be within the family Oocystaceae (Trebouxiophyceae). However, research on its ultrastructure is rare, and the phylogenetic position has not yet been determined. In this study, two strains of Nephrocytium, N. agardhianum Nägeli and N. limneticum (G.M.Smith) G.M.Smith, were identified and successfully cultured in the laboratory. Morphological inspection by light and electron microscopy and molecular phylogenetic analyses were performed to explore the taxonomic position. Ultrastructure implied a likely irregular network of dense and fine ribs on the surface of the daughter cell wall that resembled that of the genus Chromochloris Kol & Chodat (Chromochloridaceae). Phylogenetic analyses revealed that Nephrocytium formed an independent lineage in the order Sphaeropleales (Chlorophyceae) with high support values and a close phylogenetic relationship with Chromochloris. Based on combined morphological, ultrastructural and phylogenetic data, we propose a re-classification of Nephrocytium into Sphaeropleales, sharing a close relationship with Chromochloris.

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Five new morphological types of virgulate and microcotylous xiphidiocercariae based on morphological and molecular phylogenetic analyses

Journal of Helminthology ◽

10.1017/s0022149x19000853 ◽

2019 ◽

Vol 94 ◽

Cited By ~ 2

Author(s):

S.V. Shchenkov ◽

S.A. Denisova ◽

G.A. Kremnev ◽

A.A. Dobrovolskij

Keyword(s):

Phylogenetic Analyses ◽

Morphological Characters ◽

Molecular Data ◽

Phylogenetic Position ◽

The Family ◽

Family Level ◽

Molecular Phylogenetic ◽

Morphological And Molecular Data ◽

First Time

Abstract The phylogenetic position of most xiphidiocercariae from subgroups Cercariae virgulae and Cercariae microcotylae remains unknown or unclear, even at the family level. In this paper, we studied the morphology and molecular phylogeny of 15 microcotylous and virgulate cercariae (11 new and four previously described ones). Based on morphological and molecular data, we suggested five distinct morphological types of xiphidiocercariae, which are a practical alternative to Cercariae virgulae and Cercariae microcotylae subgroups. Four of these types correspond to actual digenean taxa (Microphallidae, Lecithodendriidae, Pleurogenidae and Prosthogonimidae), while the fifth is represented by Cercaria nigrospora Wergun, 1957, which we classified on the basis of molecular data for the first time. We reassessed the relative importance of morphological characters used for the classification of virgulate and microcotylous cercariae, and discussed the main evolutionary trends within xiphidiocercariae. Now stylet cercariae can be reliably placed into several sub-taxa of Microphalloidea on the basis of their morphological features.

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The Phylogenetic Position of Crenea in the Lythraceae Based on Molecular Evidence, and the Transfer of Its Two Species to Ammannia

Annals of the Missouri Botanical Garden ◽

10.3417/2021629 ◽

2021 ◽

Vol 106 ◽

pp. 325-339

Author(s):

Shirley A. Graham ◽

Peter W. Inglis ◽

Taciana B. Cavalcanti

Keyword(s):

Morphological Character ◽

Phylogenetic Analyses ◽

Taxonomic Status ◽

Phylogenetic Position ◽

Molecular Evidence ◽

Monophyletic Lineage ◽

The Family ◽

Mangrove Vegetation ◽

Molecular Phylogenetic ◽

Globally Distributed

Crenea Aubl. (Lythraceae) is a ditypic genus of subshrubs occurring in mangrove vegetation on the coasts of northern South America. Phylogenetic analyses based on morphology have offered unresolved and conflicting phylogenetic positions for the genus in the family. This study presents the first molecular sequences for Crenea, from nrITS, rbcL, trnL, trnL-F, and matK regions. Molecular phylogenetic analyses find full support for Crenea within Ammannia L., a relationship not previously recognized. Ammannia is a globally distributed genus of terrestrial to amphibious herbs mostly occurring in freshwater marshes and wetlands. It was recently reconfigured based on phylogenetic evidence to include the genera Nesaea Comm. ex Kunth and Hionanthera A. Fern. & Diniz. The transfer of Crenea to Ammannia further extends the morphological, ecological, and biogeographical diversity of Ammannia and provides the final evidence defining Ammannia as a monophyletic lineage of the Lythraceae. A revised circumscription of Ammannia s.l. adds several new morphological character states and the first species in the genus restricted to mangrove vegetation. Two changes in taxonomic status are made: Ammannia maritima (Aubl.) S. A. Graham, P. W. Inglis, & T. B. Cavalc., comb. nov., and Ammannia patentinervius (Koehne) S. A. Graham, P. W. Inglis, & T. B. Cavalc., comb. nov. The new combinations are described, a list of exsiccatae examined is provided, and the effects of the reconfiguration to the morphology and biogeography of the genus are detailed.

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A new predatory leech from Vietnam (Hirudinida : Arhynchobdellida : Salifidae): its phylogenetic position with comments on the classification of the family

Invertebrate Systematics ◽

10.1071/is15008 ◽

2015 ◽

Vol 29 (5) ◽

pp. 473 ◽

Cited By ~ 1

Author(s):

Takafumi Nakano ◽

Son Truong Nguyen

Keyword(s):

Phylogenetic Relationships ◽

Phylogenetic Analyses ◽

Morphological Characteristics ◽

Molecular Data ◽

Phylogenetic Position ◽

12S Rrna ◽

The Family ◽

Morphological And Molecular Data ◽

First Time

The family Salifidae is a predaceous leech taxon in the suborder Erpobdelliformes. Although Salifidae is widely distributed in the African, Oriental, Indo-Malayan, Sino-Japanese and Australasian regions, the phylogenetic relationships of the family Salifidae have never been tested using molecular data obtained from leeches collected from the family distributional range. A salifid species was collected for the first time in Vietnam, and relevant morphological and molecular data are presented here. Because the Vietnamese salifid species possesses unique morphological characteristics among the known salifid species, this species is herein described as a new species, Salifa motokawai, sp. nov. Phylogenetic analyses based on nuclear 18S rRNA and histone H3, as well as mitochondrial cytochrome c oxidase subunit I, tRNACys, tRNAMet, 12S rRNA, tRNAVal, 16S rRNA, tRNALeu and NADH dehydrogenase subunit 1 markers demonstrate that the Vietnamese salifid species is a close congener with the African Salifa perspicax and the Malagasy Linta be. Furthermore, molecular data revealed non-monophyly of the Asian salifid leeches. According to the observed phylogenetic relationships and morphological characteristics of the Vietnamese Salifa motokawai, sp. nov., the current classification of salifid taxa should be revised.

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Phylogenomic analysis and revised classification of atypoid mygalomorph spiders (Araneae, Mygalomorphae), with notes on arachnid ultraconserved element loci

PeerJ ◽

10.7717/peerj.6864 ◽

2019 ◽

Vol 7 ◽

pp. e6864 ◽

Cited By ~ 20

Author(s):

Marshal Hedin ◽

Shahan Derkarabetian ◽

Adan Alfaro ◽

Martín J. Ramírez ◽

Jason E. Bond

Keyword(s):

Species Diversity ◽

Phylogenetic Analyses ◽

Divergence Time ◽

Divergence Times ◽

Phylogenomic Analysis ◽

Early Tertiary ◽

The Family ◽

Molecular Phylogenetic ◽

Probe Set

The atypoid mygalomorphs include spiders from three described families that build a diverse array of entrance web constructs, including funnel-and-sheet webs, purse webs, trapdoors, turrets and silken collars. Molecular phylogenetic analyses have generally supported the monophyly of Atypoidea, but prior studies have not sampled all relevant taxa. Here we generated a dataset of ultraconserved element loci for all described atypoid genera, including taxa (MecicobothriumandHexurella)key to understanding familial monophyly, divergence times, and patterns of entrance web evolution. We show that the conserved regions of the arachnid UCE probe set target exons, such that it should be possible to combine UCE and transcriptome datasets in arachnids. We also show that different UCE probes sometimes target the same protein, and under the matching parameters used here show that UCE alignments sometimes include non-orthologs. Using multiple curated phylogenomic matrices we recover a monophyletic Atypoidea, and reveal that the family Mecicobothriidae comprises four separate and divergent lineages. Fossil-calibrated divergence time analyses suggest ancient Triassic (or older) origins for several relictual atypoid lineages, with late Cretaceous/early Tertiary divergences within some genera indicating a high potential for cryptic species diversity. The ancestral entrance web construct for atypoids, and all mygalomorphs, is reconstructed as a funnel-and-sheet web.

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The phylogenetic position of Artematopodidae (Coleoptera: Elateroidea), with description of the first two Eurypogon species from China

Contributions to Zoology ◽

10.1163/18759866-08204004 ◽

2013 ◽

Vol 82 (4) ◽

pp. 199-221 ◽

Cited By ~ 5

Author(s):

Robin Kundrata ◽

Milada Bocakova ◽

Ladislav Bocak

Keyword(s):

Molecular Markers ◽

Phylogenetic Analyses ◽

Female Genitalia ◽

Phylogenetic Position ◽

The Family ◽

Molecular Phylogenetic ◽

Basal Position

The Artematopodidae is a species-poor beetle family with contentious relationships to byrrhoid and elateroid families. Recent molecular phylogenetic analyses brought ambiguous results based on a single sequenced species. We investigated the taxonomic placement of Artematopodidae within Elateriformia using ribosomal (18S, 28S) and mitochondrial (rrnL, cox1) molecular markers and three artematopodid species. Our analyses placed Artematopodidae close to Omethidae+Telegeusidae in a basal position of broadly defined Elateroidea. Additionally, we described the first artematopodid species from China – Eurypogon jaechi sp. nov. and E. heishuiensis sp. nov. These species are reported from mountains of Yunnan and can be easily distinguished from their Palaearctic congeners by their large bodies and metallic green elytra. They differ from each other by the shape of the pronotum, puncturation of the head and pronotum, the relative lengths of the antennomeres 3-5, and the morphology of the female genitalia. With respect to our findings, we discussed the phylogeny, diversity and distribution of the family Artematopodidae.

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Tracing the origins of hybrids through history: monstrous cultivars and Napoléon Bonaparte’s exiled paper daisies (Asteraceae; Gnaphalieae)

Botanical Journal of the Linnean Society ◽

10.1093/botlinnean/boab020 ◽

2021 ◽

Author(s):

Timothy L Collins ◽

Jeremy J Bruhl ◽

Alexander N Schmidt-Lebuhn ◽

Ian R H Telford ◽

Rose L Andrew

Keyword(s):

Gene Flow ◽

Phylogenetic Analyses ◽

Strong Support ◽

Napoleon Bonaparte ◽

Snp Data ◽

Cast Doubt ◽

Molecular Phylogenetic ◽

St Helena ◽

New Evidence

Abstract Golden everlasting paper daisies (Xerochrysum, Gnaphalieae, Asteraceae) were some of the earliest Australian native plants to be cultivated in Europe. Reputedly a favourite of Napoléon Bonaparte and Empress Joséphine, X. bracteatum is thought to have been introduced to the island of St Helena in the South Atlantic during Napoléon’s exile there. Colourful cultivars were developed in the 1850s, and there is a widely held view that these were produced by crossing Xerochrysum with African or Asian Helichrysum spp. Recent molecular phylogenetic analyses and subtribal classification of Gnaphalieae cast doubt on this idea. Using single-nucleotide polymorphism (SNP) data, we looked for evidence of gene flow between modern cultivars, naturalized paper daisies from St Helena and four Xerochrysum spp. recorded in Europe in the 1800s. There was strong support for gene flow between cultivars and X. macranthum. Paper daisies from St Helena were genotypically congruent with X. bracteatum and showed no indications of ancestry from other species or from the cultivars, consistent with the continuous occurrence of naturalized paper daisies introduced by Joséphine and Napoléon. We also present new evidence for the origin of colourful Xerochrysum cultivars and hybridization of congeners in Europe from Australian collections.

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Resolving the Lourinia armata (Claus, 1866) complex with remarks on the monophyletic status of Louriniidae, Monard 1927 (Copepoda: Harpacticoida)

Zootaxa ◽

10.11646/zootaxa.5051.1.15 ◽

2021 ◽

Vol 5051 (1) ◽

pp. 346-386

Author(s):

SÜPHAN KARAYTUĞ ◽

SERDAR SAK ◽

ALP ALPER ◽

SERDAR SÖNMEZ

Keyword(s):

Comparative Evaluation ◽

Species Complex ◽

Detailed Examination ◽

The Other ◽

Phylogenetic Position ◽

Nicobar Island ◽

Other Hand ◽

The Family ◽

Close Relationship ◽

Almost All

An attempt was made to test if Lourinia armata (Claus, 1866)—as it is currently diagnosed—represents a species complex. Detailed examination and comparisons of several specimens collected from different localities suggest that L. armata indeed represents a complex of four closely related morphospecies that can be differentiated from one another by only detailed observations. One of the four species is identified as Lourinia aff. armata and the other three species are described as new to science and named as Lourinia wellsi sp. nov., L. gocmeni sp. nov., and L. aldabraensis sp. nov. Detailed review of previous species records indicates that the genus Lourinia Wilson, 1924 is distributed worldwide. Ceyloniella nicobarica Sewell, 1940, originally described from Nicobar Island and previously considered a junior subjective synonym of L. armata is reinstated as Lourinia nicobarica (Sewell, 1940) comb. nov. on the basis of the unique paddle-shaped caudal ramus seta V. It is postulated that almost all of these records are unreliable in terms of representing true Lourinia aff. armata described herein. On the other hand, the comparative evaluation of the illustrations and descriptions in the published literature indicates the presence of several new species waiting to be discovered in the genus Lourinia. It has been determined that, according to updated modern keys, the recent inclusion of the monotypic genus Archeolourinia Corgosinho & Schizas, 2013 in the Louriniidae is not justified since Archeolourinia shermani Corgosinho & Schizas, 2013 does not belong to this family but should be assigned to the Canthocamptidae. On the other hand, it has been argued that the exact phylogenetic position of the Louriniidae still remains problematic since none of the diagnostic characters supports the monophyly of the family within the Oligoarthra. It has also been argued that the close relationship between Louriniidae and Canthocamptidae is supported since both families share the homologous sexual dimorphism (apophysis) on P3 endopod. The most important characteristic that can possibly be used to define Louriniidae is the reduction of maxilliped.

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Morphology of a unique ophidiid, Hypopleuron caninum Radcliffe 1913 (Ophidiiformes, Ophidiidae, Neobythitinae), suggesting a close relationship with the family Carapidae

Zootaxa ◽

10.11646/zootaxa.4521.4.2 ◽

2018 ◽

Vol 4521 (4) ◽

pp. 499

Author(s):

SHINPEI OHASHI

Keyword(s):

Phylogenetic Position ◽

Dorsal Fin ◽

The Family ◽

Close Relationship ◽

Pelvic Fin

Osteological and myological characters of the ophidiid Hypopleuron caninum are described here in detail. In addition to well-known characters of Ophidiidae (e.g., anal-fin origin well posterior to dorsal-fin origin; barbel-like pelvic fin; many scales), many unique or rare conditions were also recognized, including predorsal bone and maxilla condition, and canine teeth on the premaxilla, which were suggestive of a relationship with Carapidae. Furthermore, the predorsal bone appear to be homologous with the vexillar support, a carapid synapomorphy. Although several characters differ from carapid counterparts (e.g., more anterior anal-fin origin and scales absent in Carapidae), some intermediate conditions between Ophidiidae and Carapidae are apparent in Hypopleuron caninum, which may therefore occupy an intermediate phylogenetic position between the two families. A unique morphology of small second infraorbital bone is recognized in all ophidiiform species observed in this study, and it may be a synapomorphic character of the order Ophidiiformes.

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Noteworthy record of the Ethiopian genet, Genetta abyssinica, (Carnivora, Viverridae) from Djibouti informs its phylogenetic position within Genetta

Mammalia ◽

10.1515/mammalia-2017-0081 ◽

2019 ◽

Vol 83 (2) ◽

pp. 180-189 ◽

Cited By ~ 2

Author(s):

Adam W. Ferguson ◽

Houssein R. Roble ◽

Molly M. McDonough

Keyword(s):

Phylogenetic Relationships ◽

Sequence Data ◽

Alternative Hypothesis ◽

Phylogenetic Analyses ◽

Phylogenetic Position ◽

Sister Relationship ◽

Molecular Sequence Data ◽

Molecular Sequence ◽

Molecular Phylogenetic ◽

Relationship Of

AbstractThe molecular phylogeny of extant genets (Carnivora, Viverridae,Genetta) was generated using all species with the exception of the Ethiopian genetGenetta abyssinica. Herein, we provide the first molecular phylogenetic assessment ofG. abyssinicausing molecular sequence data from multiple mitochondrial genes generated from a recent record of this species from the Forêt du Day (the Day Forest) in Djibouti. This record represents the first verified museum specimen ofG. abyssinicacollected in over 60 years and the first specimen with a specific locality for the country of Djibouti. Multiple phylogenetic analyses revealed conflicting results as to the exact relationship ofG. abyssinicato otherGenettaspecies, providing statistical support for a sister relationship to all other extant genets for only a subset of mitochondrial analyses. Despite the inclusion of this species for the first time, phylogenetic relationships amongGenettaspecies remain unclear, with limited nodal support for many species. In addition to providing an alternative hypothesis of the phylogenetic relationships among extant genets, this recent record provides the first complete skeleton of this species to our knowledge and helps to shed light on the distribution and habitat use of this understudied African small carnivore.

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Polulichloris henanensis gen. et sp. nov. (Trebouxiophyceae, Chlorophyta), a novel subaerial coccoid green alga

Phytotaxa ◽

10.11646/phytotaxa.218.2.3 ◽

2015 ◽

Vol 218 (2) ◽

pp. 137 ◽

Cited By ~ 10

Author(s):

Huiyin Song ◽

Qi Zhang ◽

Guoxiang Liu ◽

Zhengyu Hu

Keyword(s):

Green Alga ◽

New Genus ◽

Cultured Cells ◽

Phylogenetic Analyses ◽

Light And Electron Microscopy ◽

Molecular Data ◽

Ribulose Bisphosphate Carboxylase ◽

Bisphosphate Carboxylase ◽

Molecular Phylogenetic ◽

18S Rdna Gene

Coccoid green algae are abundant in subaerial habitats, but they are largely unexplored because of their morphological uniformity. Several new genus-level lineages have recently been described on the basis of molecular data. In this study, a coccoid green alga was isolated from surface soil in Zhoukou, Henan Province, China, and the cultured cells were described using light and electron microscopy. The ellipsoidal cell had smooth cell wall and parietal chloroplast with a pyrenoid surrounded by a starch envelope. Reproduction occurred by formation of 2‒16 autospores. Molecular phylogenetic analyses based on the nuclear 18S rDNA gene and the chloroplast ribulose-bisphosphate carboxylase gene (rbcL) indicated that this coccoid green alga represents a new lineage of the Watanabea clade (Trebouxiophyceae, Chlorophyta). Here, we describe this organism as a new genus and species, Polulichloris henanensis, gen. et sp. nov.

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