scholarly journals Trypanosoma (Herpetosoma) diversity in rodents and lagomorphs of New Mexico with a focus on epizootological aspects of infection in Southern Plains woodrats (Neotoma micropus)

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0244803
Author(s):  
Irina Goodrich ◽  
Clifton McKee ◽  
Michael Kosoy
Keyword(s):  
New Mexico ◽  
Natural Populations ◽  
Wild Rodents ◽  
Southern Plains ◽  
Geographic Ranges ◽  
Dipodomys Ordii ◽  
Infection Dynamics ◽  
Pcr Targeting ◽  
Insight Into ◽  
Host Associations

Protozoan parasites of the genus Trypanosoma infect a broad diversity of vertebrates and several species cause significant illness in humans. However, understanding of the phylogenetic diversity, host associations, and infection dynamics of Trypanosoma species in naturally infected animals is incomplete. This study investigated the presence of Trypanosoma spp. in wild rodents and lagomorphs in northern New Mexico, United States, as well as phylogenetic relationships among these parasites. A total of 458 samples from 13 rodent and one lagomorph species collected between November 2002 and July 2004 were tested by nested PCR targeting the 18S ribosomal RNA gene (18S rRNA). Trypanosoma DNA was detected in 25.1% of all samples, with the highest rates of 50% in Sylvilagus audubonii, 33.1% in Neotoma micropus, and 32% in Peromyscus leucopus. Phylogenetic analysis of Trypanosoma sequences revealed five haplotypes within the subgenus Herpetosoma (T. lewisi clade). Focused analysis on the large number of samples from N. micropus showed that Trypanosoma infection varied by age class and that the same Trypanosoma haplotype could be detected in recaptured individuals over multiple months. This is the first report of Trypanosoma infections in Dipodomys ordii and Otospermophilus variegatus, and the first detection of a haplotype phylogenetically related to T. nabiasi in North America in S. audubonii. This study lends important new insight into the diversity of Trypanosoma species, their geographic ranges and host associations, and the dynamics of infection in natural populations.

scholarly journals Contrasting Patterns of Virus Protection and Functional Incompatibility Genes in Two ConspecificWolbachiaStrains fromDrosophila pandora

2018 ◽  
Vol 85 (5) ◽  
Author(s):  
Angelique K. Asselin ◽  
Simon Villegas-Ospina ◽  
Ary A. Hoffmann ◽  
Jeremy C. Brownlie ◽  
Karyn N. Johnson
Keyword(s):  
Disease Transmission ◽  
Cytoplasmic Incompatibility ◽  
Natural Populations ◽  
Differential Protection ◽  
Content Type ◽  
Infection Dynamics ◽  
Modification Factor ◽  
Control Disease ◽  
Multiple Strains ◽  
High Degree

ABSTRACTWolbachiainfections can present different phenotypes in hosts, including different forms of reproductive manipulation and antiviral protection, which may influence infection dynamics within host populations. In populations ofDrosophila pandoratwo distinctWolbachiastrains coexist, each manipulating host reproduction: strainwPanCI causes cytoplasmic incompatibility (CI), whereas strainwPanMK causes male killing (MK). CI occurs when aWolbachia-infected male mates with a female not infected with a compatible type ofWolbachia, leading to nonviable offspring.wPanMK can rescuewPanCI-induced CI but is unable to induce CI. The antiviral protection phenotypes provided by thewPanCI andwPanMK infections were characterized; the strains showed differential protection phenotypes, whereby cricket paralysis virus (CrPV)-induced mortality was delayed in flies infected withwPanMK but enhanced in flies infected withwPanCI compared to their respectiveWolbachia-cured counterparts. Homologs of thecifAandcifBgenes involved in CI identified inwPanMK andwPanCI showed a high degree of conservation; however, the CifB protein inwPanMK is truncated and is likely nonfunctional. The presence of a likely functional CifA inwPanMK andwPanMK’s ability to rescuewPanCI-induced CI are consistent with the recent confirmation of CifA’s involvement in CI rescue, and the absence of a functional CifB protein further supports its involvement as a CI modification factor. Taken together, these findings indicate thatwPanCI andwPanMK have different relationships with their hosts in terms of their protective and CI phenotypes. It is therefore likely that different factors influence the prevalence and dynamics of these coinfections in naturalDrosophila pandorahosts.IMPORTANCEWolbachiastrains are common endosymbionts in insects, with multiple strains often coexisting in the same species. The coexistence of multiple strains is poorly understood but may rely onWolbachiaorganisms having diverse phenotypic effects on their hosts. AsWolbachiais increasingly being developed as a tool to control disease transmission and suppress pest populations, it is important to understand the ways in which multipleWolbachiastrains persist in natural populations and how these might then be manipulated. We have therefore investigated viral protection and the molecular basis of cytoplasmic incompatibility in two coexistingWolbachiastrains with contrasting effects on host reproduction.

New Mexico Obsidian Sources and Exchange on the Southern Plains

1987 ◽  
Vol 14 (3) ◽  
pp. 313-329 ◽  
Author(s):  
Timothy G. Baugh ◽  
Fred W. Nelson
Keyword(s):  
New Mexico ◽  
Southern Plains

scholarly journals Haemoplasmas in wild rodents: Routes of transmission and infection dynamics

2018 ◽  
Vol 27 (18) ◽  
pp. 3714-3726 ◽  
Author(s):  
Carmit Cohen ◽  
Merav Shemesh ◽  
Mario Garrido ◽  
Irit Messika ◽  
Monica Einav ◽  
...  
Keyword(s):  
Wild Rodents ◽  
Infection Dynamics

scholarly journals Pervasive Phenotypic Impact of a Large Nonrecombining Introgressed Region in Yeast

2020 ◽  
Vol 37 (9) ◽  
pp. 2520-2530 ◽  
Author(s):  
Christian Brion ◽  
Claudia Caradec ◽  
David Pflieger ◽  
Anne Friedrich ◽  
Joseph Schacherer
Keyword(s):  
Mating Type ◽  
Budding Yeast ◽  
Natural Populations ◽  
Phenotypic Effect ◽  
Genetic Characteristics ◽  
Type Locus ◽  
First Time ◽  
The Relationship ◽  
Insight Into ◽  
Introgressed Region

Abstract To explore the origin of the diversity observed in natural populations, many studies have investigated the relationship between genotype and phenotype. In yeast species, especially in Saccharomyces cerevisiae, these studies are mainly conducted using recombinant offspring derived from two genetically diverse isolates, allowing to define the phenotypic effect of genetic variants. However, large genomic variants such as interspecies introgressions are usually overlooked even if they are known to modify the genotype–phenotype relationship. To have a better insight into the overall phenotypic impact of introgressions, we took advantage of the presence of a 1-Mb introgressed region, which lacks recombination and contains the mating-type determinant in the Lachancea kluyveri budding yeast. By performing linkage mapping analyses in this species, we identified a total of 89 loci affecting growth fitness in a large number of conditions and 2,187 loci affecting gene expression mostly grouped into two major hotspots, one being the introgressed region carrying the mating-type locus. Because of the absence of recombination, our results highlight the presence of a sexual dimorphism in a budding yeast for the first time. Overall, by describing the phenotype–genotype relationship in the Lachancea kluyveri species, we expanded our knowledge on how genetic characteristics of large introgression events can affect the phenotypic landscape.

scholarly journals Hantavirus infections in fluctuating host populations: the role of maternal antibodies

2010 ◽  
Vol 277 (1701) ◽  
pp. 3783-3791 ◽  
Author(s):  
Eva R. Kallio ◽  
Michael Begon ◽  
Heikki Henttonen ◽  
Esa Koskela ◽  
Tapio Mappes ◽  
...  
Keyword(s):  
Breeding Season ◽  
Natural Populations ◽  
Late Summer ◽  
Maternal Antibodies ◽  
Infection Prevalence ◽  
Infection Dynamics ◽  
High Infection ◽  
Maternal Protection ◽  
First Time

Infected females may transfer maternal antibodies (MatAbs) to their offspring, which may then be transiently protected against infections the mother has encountered. However, the role of maternal protection in infectious disease dynamics in wildlife has largely been neglected. Here, we investigate the effects of Puumala hantavirus (PUUV)-specific MatAbs on PUUV dynamics, using 7 years' data from a cyclic bank vole population in Finland. For the first time to our knowledge, we partition seropositivity data from a natural population into separate dynamic patterns for MatAbs and infection. The likelihood of young of the year carrying PUUV-specific MatAbs during the breeding season correlated positively with infection prevalence in the overwintered parent population in the preceding spring. The probability of PUUV infection varied between seasons (highest in spring, lowest in late summer) and depended on population structure, but was also, in late autumn, notably, negatively related to summer MatAb prevalence, as well as to infection prevalence earlier in the breeding season. Hence, our results suggest that high infection prevalence in the early breeding season leads to a high proportion of transiently immune young individuals, which causes delays in transmission. This suggests, in turn, that MatAb protection has the potential to affect infection dynamics in natural populations.

Clonal Analysis of IgM Cells in Multiple Myeloma Patients Suggests the Co-Existence of Normal and Malignant Arms of the Myeloma Clone.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 1415-1415
Author(s):  
Brian J. Taylor ◽  
Ming Ye ◽  
Erin R. Strachan ◽  
Tara M. Tiffinger ◽  
Andrew R. Belch ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
B Cell ◽  
Plasma Cells ◽  
Somatic Hypermutation ◽  
Immunomagnetic Separation ◽  
B Cell Differentiation ◽  
Reading Frame ◽  
A Minor ◽  
Pcr Targeting ◽  
Insight Into

Abstract Analysis of immunoglobulin V genes, which undergo stepwise changes during B cell differentiation such as VDJ rearrangement, somatic hypermutation, and class switch recombination, provides insight into the point of transformation of B cell tumors. In Multiple Myeloma (MM), clonotypic VDJ sequences of malignant plasma cells are mutated, homogeneous, and associated with post-switch constant regions (either IgG or IgA, called the clinical isotype), suggesting the malignant arm of the MM clone arises from transformation events in the late stages of the germinal centre reaction. By contrast, the existence of clonotypic VDJ associated with pre-switch IgM is well established, and we have shown persistent clonotypic IgM is associated with advanced disease at diagnosis and poor survival in MM. Whether clonotypic IgM cells represent a malignant progenitor or a non-malignant population that parallels disease severity is unclear. To address these possibilities, we focused our analysis of clonotypic VDJ mutation profiles on IgM+ cells sorted by immunomagnetic separation from MM patient peripheral blood cells (PBMC). IgM clonotypic transcripts were amplified by hemi-nested RT-PCR targeting the CDR2-C mu constant region in IgM+ cells from 4/7 patients. These products were cloned, and 122, 28, 27, and 25 IgM clonotypic colonies were identified by specific CDR2/CDR3 PCR for patients 1–4 respectively. Each of these clones was sequenced, and mutations were identified by comparison with the closest germline V gene and tumor derived plasma cell VDJ sequences. An average mutation frequency of 0.005, significantly greater than the Taq error rate, was obtained for the 250–280 bp fragment downstream of CDR2, including the D-J-C mu region. Typically, MM clones were observed with 1–2 mutations in this region, many localizing to the D-J-C mu region. Small deletions that preserve reading frame were also observed in the D region of single clones of patients 1 and 4 respectively. The detection of intraclonal heterogeneity amongst clonotypic IgM cells may reflect a normal arm of the myeloma clone that co-exists with the post-switch malignant arm. In previous work examining bulk PBMC populations we had detected diversified clonotypic cells in the non-clinical isotype compartment of one patient, but, in accordance with studies performed by several other groups, were unable to detect diversified pre-switch counterparts. In this work we have focused on IgM+ MM B cells, a compartment of the MM clone that may remain driven by antigenic selection and undergo persistent clonal expansion. Our analysis gives insight into the nature of this proposed normal arm of the myeloma clone, revealing two coexisting subsets of pre-switch clonotypic IgM cells: a major set exhibiting homogeneity, identity with post-switch tumor VDJ, and questionable transformation status, and a minor clonally heterogeneous set which may represent the pre-malignant clone from which myeloma arose.

scholarly journals Adaptive plasticity – its definition, measurement, and importance

2021 ◽  
Author(s):  
Rob Brooker ◽  
Lawrie K Brown ◽  
Timothy S. George ◽  
Robin J. Pakeman ◽  
Sarah Palmer ◽  
...  
Keyword(s):  
Evolutionary Biology ◽  
Natural Populations ◽  
Plant Ecology ◽  
Adaptive Plasticity ◽  
Plant Fitness ◽  
First Line ◽  
Growth And Survival ◽  
Plant Sciences ◽  
Insight Into ◽  
Climate Change Resilience

Plasticity is a widely used concept in plant sciences, but there is inconsistency over its interpretation and measurement. One aspect of plasticity – adaptive plasticity – may be particularly important in shaping plant fitness and reproductive success and represents the first line of a plants defence to environmental change. Here, we define adaptive plasticity, highlight its importance to plant growth and survival, and suggest appropriate approaches for its measurement. We argue that a focus on adaptive plasticity could help address some fundamental challenges in plant ecology and evolutionary biology, including developing insight into climate-change resilience of natural populations and crops.

scholarly journals Pervasive phenotypic impact of a large non-recombining introgressed region in yeast

2020 ◽  
Author(s):  
Christian Brion ◽  
Claudia Caradec ◽  
David Pflieger ◽  
Anne Friedrich ◽  
Joseph Schacherer
Keyword(s):  
Mating Type ◽  
Budding Yeast ◽  
Natural Populations ◽  
Phenotypic Effect ◽  
Genetic Characteristics ◽  
Type Locus ◽  
First Time ◽  
The Relationship ◽  
Insight Into ◽  
Introgressed Region

AbstractTo explore the origin of the diversity observed in natural populations, many studies have investigated the relationship between genotype and phenotype. In yeast species, especially in Saccharomyces cerevisiae, these studies are mainly conducted using recombinant offspring derived from two genetically diverse isolates, allowing to define the phenotypic effect of genetic variants. However, large genomic variants such as interspecies introgressions are usually overlooked even if they are known to modify the genotype-phenotype relationship. To have a better insight into the overall phenotypic impact of introgressions, we took advantage of the presence of a 1-Mb introgressed region, which lacks recombination and contains the mating-type determinant in the Lachancea kluyveri budding yeast. By performing linkage mapping analyses in this species, we identified a total of 89 loci affecting growth fitness in a large number of conditions and 2,187 loci affecting gene expression mostly grouped into two major hotspots, one being the introgressed region carrying the mating-type locus. Because of the absence of recombination, our results highlight the presence of a sexual dimorphism in a budding yeast for the first time. Overall, by describing the phenotype-genotype relationship in the L. kluyveri species, we expanded our knowledge on how genetic characteristics of large introgression events can affect the phenotypic landscape.

INSIGHT INTO SMALL-VOLUME VOLCANIC FIELDS IN CENTRAL RIO GRANDE RIFT—ALBUQUERQUE VOLCANOES, NEW MEXICO

2016 ◽  
Author(s):  
Christine F. Chan ◽  
◽  
Ren A. Thompson ◽  
Matthew J. Zimmerer ◽  
Wayne R. Premo ◽  
...  
Keyword(s):  
New Mexico ◽  
Small Volume ◽  
Rio Grande ◽  
Rio Grande Rift ◽  
Volcanic Fields ◽  
Insight Into

scholarly journals Geographical Distribution of Borrelia burgdorferi sensu lato in Ticks Collected from Wild Rodents in the Republic of Korea

Pathogens ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 866
Author(s):  
Seong Yoon Kim ◽  
Tae-Kyu Kim ◽  
Tae Yun Kim ◽  
Hee Il Lee
Keyword(s):  
Sequence Analysis ◽  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Jeju Island ◽  
Republic Of Korea ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Wild Rodents ◽  
The Republic ◽  
Tick Vectors ◽  
Pcr Targeting

Lyme disease is a tick-borne zoonotic disease caused by Borrelia burgdorferi sensu lato (s. l.) via transmission cycles involving competent tick vectors and vertebrate reservoirs. Here, we determined the prevalence and distribution of Borrelia genospecies in 738 ticks of at least three species from wild rodents in nine regions of the Republic of Korea (ROK). Ticks were analyzed using nested PCR targeting partial flagellin B gene sequences, followed by sequence analysis. The prevalence of Borrelia infection was 33.6%, and the most common genospecies were B. afzelii (62.5%), B. valaisiana (31.9%), B. yangtzensis (2.4%), B. garinii (1.6%), and B. tanukii (1.6%). Borrelia afzelii was found in all regions except Jeju Island; this predominant genospecies was found in the northern and central sampling regions. Borrelia valaisiana, B. yangtzensis, and B. tanukii were found only in the southern regions with B. valaisiana being the most common, whereas B. yangtzensis and B. tanukii were only found on Jeju Island. Our study is the first to describe the nationwide prevalence of B. burgdorferi s. l. in ticks from wild rodents in the ROK. Continuous surveillance in ticks, animals, humans, and different regions is required to avoid disease distribution and possible transmission to humans in the ROK.

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