scholarly journals Light-Induced Byproducts of Vitamin C in Multivitamin Solutions

2004 ◽  
Vol 50 (1) ◽  
pp. 135-140 ◽  
Author(s):  
Jean-Claude Lavoie ◽  
Philippe Chessex ◽  
Thérèse Rouleau ◽  
Diane Migneault ◽  
Blandine Comte
Keyword(s):  
Mass Spectrometry ◽  
Ascorbic Acid ◽  
Vitamin C ◽  
Organic Peroxides ◽  
Ambient Light ◽  
Rapid Loss ◽  
Biological Importance ◽  
Before And After

Abstract Background: When solutions of multivitamin preparations (MVPs) are exposed to light, H2O2 as well as organic peroxides are generated and the concentration of vitamin C decreases. The aim of this study was to determine, using mass spectrometry, whether the generation of oxidative byproducts of vitamin C, such as dehydroascorbate (DHA) and 2,3-diketogulonic acid (DKG), accounted for the reported decrease in ascorbic acid in MVPs exposed to light. Methods: Mass spectrometry was used to document the formation of byproducts of ascorbic acid in solutions containing a MVP, vitamin C + riboflavin, and vitamin C + H2O2 + Fe2+. The involvement of ascorbic acid and H2O2 in the formation of organic peroxides was tested by measuring peroxide concentrations in solutions containing H2O2 with or without ascorbic acid and with or without Fe2+ before and after addition of catalase. Results: The loss of ascorbic acid in photo-exposed MVPs was associated with the concomitant generation of byproducts different from DHA and DKG. Among them, one mass fingerprint was particularly observed with solutions of vitamin C + riboflavin exposed to ambient light as well as with the solution of vitamin C + H2O2 + Fe2+, suggesting a Fenton-like reaction. This fingerprint was associated with the formation of catalase-resistant peroxides. Conclusion: Exposure of MVPs to light leads to the rapid loss of ascorbic acid and generation of specific byproducts that differ from DHA and DKG. The conversion of vitamin C into byproducts could be of biological importance in accounting for the decrease in ascorbic acid concentrations and the generation of organic peroxides in light-exposed MVPs.

scholarly journals Effect of ascorbic acid on Mycobacterium tuberculosis biofilms

2014 ◽  
Author(s):  
James P Dalton ◽  
Benedict Uy ◽  
Simon Swift ◽  
Siouxsie Wiles
Keyword(s):  
Ascorbic Acid ◽  
Mycobacterium Tuberculosis ◽  
Vitamin C ◽  
Biofilm Growth ◽  
Light Production ◽  
Tb Treatment ◽  
Light Levels ◽  
Before And After ◽  
Surrogate Measure ◽  
Non Destructive

Planktonic cultures of Mycobacterium tuberculosis, the bacterium responsible for the lung disease tuberculosis (TB), are highly susceptible to killing by ascorbic acid (vitamin C). As planktonically grown M. tuberculosis are unlikely to be representative of the bacterium during infection, we set out to determine if ascorbic acid was also antibacterial against M. tuberculosis growing as a biofilm. We use biofilm growth as a model for the multiple phenotypic states M. tuberculosis can exist in during an infection. In our experiments we employed bioluminescent M. tuberculosis H37Rv (BSGTB1) in which light production is a non-destructive surrogate measure of bacterial viability. Light levels were monitored before and after treatment with 1mM to 256mM ascorbic acid. After 3 weeks of treatment, biofilms were disrupted, washed and inoculated into fresh media to look for sterilisation. Our findings show that ascorbic acid concentrations of 32mM or greater reduced bioluminescence produced by M. tuberculosis BSGTB1 growing in biofilms to background levels and resulted in the death of all cells within the biofilm. This indicates that M. tuberculosis biofilms are susceptible to inhibition and killing by ascorbic acid (vitamin C) and suggests that novel antibiotics with a mode of action similar to ascorbic acid could represent a useful avenue of investigation for TB treatment.

Connective tissue studies. IV: l-ascorbic-1-C14 acid excretion in intact and wounded guinea pigs on varying vitamin C intakes

1960 ◽  
Vol 199 (3) ◽  
pp. 423-428 ◽  
Author(s):  
Susanne von Schuching ◽  
Theodore Enns ◽  
Arthur F. Abt
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Guinea Pigs ◽  
Single Injection ◽  
Scar Tissue ◽  
Simplified Method ◽  
Tissue Area ◽  
Before And After ◽  
Distant Tissue ◽  
Total Excretion

Tissues of scorbutic guinea pigs take up a greater percentage of radioactive ascorbic acid following a single injection than do the tissues of guinea pigs on higher vitamin C diets. After wounding, the scar tissue area contains a larger amount of ascorbic acid than distant tissue. Studies of total excretion following a single injection of l-ascorbic-i-C14 acid were conducted at three dietary intake levels, both before and after wounding. Results obtained show that the greatest differences from the preoperative C14 excretion occur during the immediate postoperative period, and after 36 hours the rate constants approximate. Relative to wounding, the guinea pigs on the three different dietary levels react with the same basic pattern; increased deposition of l-ascorbic-i-C14 acid in the scar tissue accompanied by less excretion of C14O2 and a slight increase in C14 activity in the urine. A simplified method for measurement of C14O2 is described. An explanation of human vitamin C requirement and a possible simple assay of ascorbic acid analogues are also suggested.

scholarly journals Evaluation of the Effect of Zinc, Quercetin, Bromelain and Vitamin C on COVID-19 Patients

2020 ◽  
Author(s):  
Amr Kamel ◽  
Heba Abdelseed ◽  
Yousef Albalawi ◽  
Eman Aslsalameen ◽  
Yousef Almutairi ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Zinc Acetate ◽  
Randomized Clinical Trials ◽  
Case Series ◽  
Phase Iii ◽  
P Value ◽  
Once Daily ◽  
Case Series Study ◽  
Before And After

AbstractCoronavirus disease 2019 (COVID-19) is an infectious disease caused by a new strain of coronavirus. There are three phases of COVID-19: early infection stage, pulmonary stage and hyper-inflammation stage respectively. It is important to prevent lung or other organs injuries by preventing phase-II and phase-III via pharmacological or non-pharmacological treatments. This was a case series study done on twenty-two patients confirmed to be infected with SARS-CoV-2 and diagnosed with COVID-19. Patients in this study have been used quercetin 800 mg, bromelain 165 mg, zinc acetate 50 mg and ascorbic acid 1 g once daily as supplements for 3 to 5 days during SARS-CoV-2 infection. The aim of this study is to evaluate the safety and efficacy of quercetin, bromelain, zinc and ascorbic acid combination supplements on patients with COVID-19. The mean levels of WBC, ANC, ALC, AMC and AST were normal among all included patients before and after taking quercetin, bromelain, zinc and ascorbic acid supplements (P-value > 0.05). Quercetin 800 mg, bromelain 165 mg, zinc acetate 50 mg and ascorbic acid 1 g once daily supplements were safe for patients infected with SARS-CoV-2 and may prevent poor prognosis. Randomized clinical trials needed in the future to ensure the efficacy of quercetin, bromelain, zinc and vitamin c combination.

scholarly journals The effect of high ascorbic acid supplementation on body iron stores

Blood ◽  
1984 ◽  
Vol 64 (3) ◽  
pp. 721-726 ◽  
Author(s):  
JD Cook ◽  
SS Watson ◽  
KM Simpson ◽  
DA Lipschitz ◽  
BS Skikne
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Serum Ferritin ◽  
Iron Status ◽  
Intestinal Adaptation ◽  
Dietary Iron ◽  
Enhancing Effect ◽  
Iron Stores ◽  
Iron Deficient ◽  
Before And After

The level of assimilation of dietary iron is believed to have an important influence on iron status. To examine the effect of enhancing the availability of dietary iron on iron balance, 17 adult volunteer subjects were given 2 g of ascorbic acid daily with meals for 16 weeks. Serum ferritin levels before and after the study averaged 46 and 43 micrograms/L, respectively, indicating a negligible effect on iron stores. When vitamin C supplementation was continued for an additional 20 months in five iron-replete and four iron-deficient subjects, serum ferritin determinations again failed to indicate any significant effect of the vitamin C on iron reserves. These findings were not explained by intestinal adaptation to the enhancing effect of the vitamin, because radioisotopic measurements of nonheme iron absorption showed no reduction in the enhancing effect of 1 g of ascorbic acid after four months of megadoses of vitamin C. It is concluded that altering the availability of nonheme dietary iron has little effect on iron status when the diet contains substantial amounts of meat.

scholarly journals Effect of ascorbic acid on Mycobacterium tuberculosis biofilms

2014 ◽  
Author(s):  
James P Dalton ◽  
Benedict Uy ◽  
Simon Swift ◽  
Siouxsie Wiles
Keyword(s):  
Ascorbic Acid ◽  
Mycobacterium Tuberculosis ◽  
Vitamin C ◽  
Biofilm Growth ◽  
Light Production ◽  
Tb Treatment ◽  
Light Levels ◽  
Before And After ◽  
Surrogate Measure ◽  
Non Destructive

Planktonic cultures of Mycobacterium tuberculosis, the bacterium responsible for the lung disease tuberculosis (TB), are highly susceptible to killing by ascorbic acid (vitamin C). As planktonically grown M. tuberculosis are unlikely to be representative of the bacterium during infection, we set out to determine if ascorbic acid was also antibacterial against M. tuberculosis growing as a biofilm. We use biofilm growth as a model for the multiple phenotypic states M. tuberculosis can exist in during an infection. In our experiments we employed bioluminescent M. tuberculosis H37Rv (BSGTB1) in which light production is a non-destructive surrogate measure of bacterial viability. Light levels were monitored before and after treatment with 1mM to 256mM ascorbic acid. After 3 weeks of treatment, biofilms were disrupted, washed and inoculated into fresh media to look for sterilisation. Our findings show that ascorbic acid concentrations of 32mM or greater reduced bioluminescence produced by M. tuberculosis BSGTB1 growing in biofilms to background levels and resulted in the death of all cells within the biofilm. This indicates that M. tuberculosis biofilms are susceptible to inhibition and killing by ascorbic acid (vitamin C) and suggests that novel antibiotics with a mode of action similar to ascorbic acid could represent a useful avenue of investigation for TB treatment.

scholarly journals EXPERIMENTAL STUDY OF THE INFLUENCE OF TEMPERATURE ON PASTEURIZATION OF PÊRA RIO IN NATURA ORANGE JUICE

2021 ◽  
Vol 29 (31) ◽  
pp. 37-42
Author(s):  
GALIASSI Gabriela Regina Rosa ◽  
RAMIREZ Maribel Valverde
Keyword(s):  
Heat Transfer ◽  
Heat Treatment ◽  
Ascorbic Acid ◽  
Heat Transfer Coefficient ◽  
Vitamin C ◽  
Central Region ◽  
Orange Juice ◽  
Convective Heat Transfer Coefficient ◽  
Dimensionless Numbers ◽  
Before And After

Background: Heat treatment is one of the most used methods to preserve food, such as orange juices, which are an excellent source of ascorbic acid. To avoid vitamin C degradation and reduce loss, fast heating is recommended. However, little is known about heat transfer during juice pasteurization. Aim: Therefore, this work aimed to determine the vitamin C content and the convective heat transfer coefficient in the pasteurization of orange juice. Methods: To perform the experiment, in the juice container, two regions were analyzed: the central region and near the wall. For the time-temperature control, thermometers were installed in the two regions mentioned. Every 120 seconds, the temperature was measured. The vitamin C content in the juice was evaluated before and after pasteurization using the iodometric method. The convective coefficient was evaluated using the method of dimensionless numbers and the experimental method. Results and Discussion: In pasteurization, the solution was heated to 80 °C, where heating lasted 3000 seconds and cooling for 2520 seconds. The graph showing the relationship of the convective heat transfer coefficient and temperature follows the same trend of the literature. The convective coefficient is higher in the region near the wall. As time passes and temperature decreases, the central region tends to equilibrium, and the coefficient becomes more constant. The vitamin C content remained constant before and after pasteurization. The values of the dimensionless numbers used in the calculations are in the same order of magnitude as the literature. Conclusions: The pasteurization did not cause ascorbic acid degradation since the heating step was fast in the heat treatment. The graphic showed that there is a dependence of the dimensionless of temperature with the dimensionless Biot and Fourier. It was noted that studying the thermal behavior in the cooling of orange juice is extremely important to ensure its quality.

scholarly journals The effect of high ascorbic acid supplementation on body iron stores

Blood ◽  
1984 ◽  
Vol 64 (3) ◽  
pp. 721-726 ◽  
Author(s):  
JD Cook ◽  
SS Watson ◽  
KM Simpson ◽  
DA Lipschitz ◽  
BS Skikne
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Serum Ferritin ◽  
Iron Status ◽  
Intestinal Adaptation ◽  
Dietary Iron ◽  
Enhancing Effect ◽  
Iron Stores ◽  
Iron Deficient ◽  
Before And After

Abstract The level of assimilation of dietary iron is believed to have an important influence on iron status. To examine the effect of enhancing the availability of dietary iron on iron balance, 17 adult volunteer subjects were given 2 g of ascorbic acid daily with meals for 16 weeks. Serum ferritin levels before and after the study averaged 46 and 43 micrograms/L, respectively, indicating a negligible effect on iron stores. When vitamin C supplementation was continued for an additional 20 months in five iron-replete and four iron-deficient subjects, serum ferritin determinations again failed to indicate any significant effect of the vitamin C on iron reserves. These findings were not explained by intestinal adaptation to the enhancing effect of the vitamin, because radioisotopic measurements of nonheme iron absorption showed no reduction in the enhancing effect of 1 g of ascorbic acid after four months of megadoses of vitamin C. It is concluded that altering the availability of nonheme dietary iron has little effect on iron status when the diet contains substantial amounts of meat.

Sorbitol enhances the physicochemical stability of B12 vitamers

2020 ◽  
Vol 90 (5-6) ◽  
pp. 439-447 ◽  
Author(s):  
Andrew Hadinata Lie ◽  
Maria V Chandra-Hioe ◽  
Jayashree Arcot
Keyword(s):  
Ascorbic Acid ◽  
Uv Light ◽  
Heat Exposure ◽  
Water Soluble ◽  
Uv Exposure ◽  
Physicochemical Stability ◽  
Before And After ◽  
The Stability

Abstract. The stability of B12 vitamers is affected by interaction with other water-soluble vitamins, UV light, heat, and pH. This study compared the degradation losses in cyanocobalamin, hydroxocobalamin and methylcobalamin due to the physicochemical exposure before and after the addition of sorbitol. The degradation losses of cyanocobalamin in the presence of increasing concentrations of thiamin and niacin ranged between 6%-13% and added sorbitol significantly prevented the loss of cyanocobalamin (p<0.05). Hydroxocobalamin and methylcobalamin exhibited degradation losses ranging from 24%–26% and 48%–76%, respectively; added sorbitol significantly minimised the loss to 10% and 20%, respectively (p < 0.05). Methylcobalamin was the most susceptible to degradation when co-existing with ascorbic acid, followed by hydroxocobalamin and cyanocobalamin. The presence of ascorbic acid caused the greatest degradation loss in methylcobalamin (70%-76%), which was minimised to 16% with added sorbitol (p < 0.05). Heat exposure (100 °C, 60 minutes) caused a greater loss of cyanocobalamin (38%) than UV exposure (4%). However, degradation losses in hydroxocobalamin and methylcobalamin due to UV and heat exposures were comparable (>30%). At pH 3, methylcobalamin was the most unstable showing 79% degradation loss, which was down to 12% after sorbitol was added (p < 0.05). The losses of cyanocobalamin at pH 3 and pH 9 (~15%) were prevented by adding sorbitol. Addition of sorbitol to hydroxocobalamin at pH 3 and pH 9 reduced the loss by only 6%. The results showed that cyanocobalamin was the most stable, followed by hydroxocobalamin and methylcobalamin. Added sorbitol was sufficient to significantly enhance the stability of cobalamins against degradative agents and conditions.

Assessment of Color Stability of Treated Leathers with Silicone Oil and Polyethylene Glycol

2014 ◽  
Vol 1 (1) ◽  
pp. 24-34
Author(s):  
Alireza K. ◽  
Hossein Ahmadi ◽  
Mohsen Mohammadi
Keyword(s):  
Ascorbic Acid ◽  
Polyethylene Glycol ◽  
Treatment Process ◽  
Color Change ◽  
Silicone Oil ◽  
Accelerated Aging ◽  
Color Stability ◽  
Antioxidant Additive ◽  
Before And After ◽  
Aesthetic Values

Lubricants and leather dressings are the most common treatments of dry and water logged historical leathers. Color change has a great importance during the time and treatment process, due to visual and aesthetic values of historic leather relics. Polyethylene glycol (PEG) and silicone oil (SiO) are frequently used leather dressings in the conservation procedures. Therefore, color stability of treated leathers with PEG and SiO were investigated before and after heat accelerated aging. Moreover, application of ascorbic acid was evaluated as an antioxidant additive for PEG (PEG+AA).Color change after treatment and aging were studied by colorimetry technique in the CIE *L*a*b system. Results indicated to severe color alteration in PEG treated and aged leathers with or without ascorbic acid. Whereas, SiO treated samples showed better stability and minimum color shift after aging. Silicone oil was characterized as the best dressing for historical leathers with compared to PEG and PEG+AA, due to its high stability and aesthetical properties.

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