scholarly journals TO ESTABLISH THE CORRELATION BETWEEN P53 AND HISTOPATHOLOGICAL GRADING OF GLIAL TUMORS

2021 ◽  
Vol 10 (4) ◽  
pp. 3421-3424
Author(s):  
Tabish Hassan
Keyword(s):  
Histopathological Examination ◽  
Histological Grade ◽  
Grading System ◽  
High Grade ◽  
Histological Grading ◽  
Biological Behaviour ◽  
P53 Expression ◽  
Glial Tumors ◽  
P53 Status ◽  
Histopathological Grading

Glial tumors occupy approximately 70% of the spectrum of all brain tumors with astrocytoma’s being the most common primary. High grade glial tumors have a poor outcome with limited survival rate. To establish the correlation between p53 status and histological grading of glial tumors. Objectives: To diagnose glial tumors on histopathological examination, to evaluate histological grade, to evaluate p53 expression and to assess the correlation between p53 expression and histological grade of gliomas. The study investigated 52 cases of gliomas. Histological grade was determined by WHO Grading System. Nuclear expression of p53 was evaluated by immunohistochemistry. A direct correlation between the histological grade and the p53 expression was observed. High grade gliomas exhibit high p53 expression. Thus, p53 as an adjunct to histological grade can provide a supportive clue to the clinicians, to predict the biological behaviour of gliomas.

scholarly journals Feline Invasive Mammary Carcinomas: Prognostic Value of Histological Grading

2019 ◽  
Vol 56 (5) ◽  
pp. 660-670 ◽  
Author(s):  
Elie Dagher ◽  
Jérôme Abadie ◽  
Delphine Loussouarn ◽  
Mario Campone ◽  
Frédérique Nguyen
Keyword(s):  
Prognostic Value ◽  
Malignant Tumors ◽  
Histological Grade ◽  
Grading System ◽  
Human Breast ◽  
Histological Grading ◽  
Cancer Specific Survival ◽  
Mammary Carcinomas ◽  
Histologic Grading ◽  
Grading Systems

Feline mammary carcinomas are highly malignant tumors usually associated with poor outcome. Nevertheless, survival times can differ significantly according to various prognostic factors. The Elston and Ellis (EE) histologic grading system, originally developed for human breast cancer, is commonly used to grade feline mammary carcinomas, although it is not really adapted for this species, hence the need of a more relevant grading system. Although few veterinary studies attempted to validate previously published results in an independent cohort, the aim of our study was to evaluate the prognostic value of different histologic grading systems in feline invasive mammary carcinomas, including the EE grading system applicable to human breast cancers and the modified and newly designed histologic grading systems recently proposed by Mills et al. Survey data and histologic features of 342 feline invasive mammary carcinomas were analyzed with respect to overall and cancer-specific survival. The histological grading system with best prognostic value was the mitotic-modified Elston and Ellis (MMEE) grading system: grade III carcinomas ( P = .04, hazard ratio [HR] = 1.46, 95% CI, 1.01–2.11), grade II ( P = .03, HR = 1.39, 95% CI, 1.03–1.88), and grade I carcinomas (HR = 1.00, reference), with decreasing hazard ratios significantly were associated with a worse overall survival, independently from the pathologic tumor size (pT ≥ 20 mm: P = .002, HR = 1.45, 95% CI, 1.15–1.83) and positive nodal stage ( P = .001, HR = 1.51, 95% CI, 1.18–1.94). This retrospective study validates Mills et al’s proposal to adapt the thresholds for mitotic counts to better assess the histological grade of the highly proliferative mammary carcinomas encountered in the cat.

scholarly journals Her2neu Amplification Associates with Co-deletion 1p/14q in Recurrent Meningiomas

2013 ◽  
Vol 40 (3) ◽  
pp. 361-365 ◽  
Author(s):  
Brenda O. Hamilton ◽  
Joanne S. Sy ◽  
Joseph F. Megyesi ◽  
Lee Cyn Ang
Keyword(s):  
Aggressive Behaviour ◽  
Histological Grade ◽  
Tissue Bank ◽  
Positive Association ◽  
Health Science ◽  
Histological Grading ◽  
Science Center ◽  
Biological Behaviour ◽  
Brain Invasion ◽  
Formalin Fixed Paraffin Embedded

Background:The current methods to predict recurrence and aggressive behaviour of meningiomas rely mainly on histological grading, histological subtype, proliferative index, as well as brain invasion. In many instances, histological grade alone fails to predict recurrence in the grade I and grade II meningiomas. Deletions of 1p and 14q have previously been reported to correlate with poor prognosis in terms of either recurrence or higher histological grades. The Her2neu (ErbB2) amplification has been shown to be a useful predictor of aggressive behaviour in breast and ovarian tumours, but its significance in meningioma is so far uncertain.Method:In order to determine the cytogenetic differences between 22 recurrent and 25 non-recurrent meningiomas of all grades, we used fluorescent in situ hybridization (FISH) DNA probes for 1p36, 14q11.2 and 17q11.2-12 (Her2neu) on formalin fixed paraffin embedded (FFPE) tissue from the Brain Tumour Tissue Bank (BTTB), London Health Science Center (LHSC).Results:We showed a positive association for meningioma recurrence correlated with 1p36 deletion plus or minus 14q 11.2 deletions in all grades of meningiomas. The Her2neu amplification was strongly associated with 1p/14q co-deletion in cases of recurrent meningiomas, especially the higher grade tumours.Conclusion:These cytogenetic markers can be applied in addition to histological grading for predicting the risk of recurrence and biological behaviour.

Prognostic significance of histological grading, p53 status, YKL-40 expression, and IDH1 mutations in pediatric high-grade gliomas

2010 ◽  
Vol 99 (2) ◽  
pp. 209-215 ◽  
Author(s):  
Manila Antonelli ◽  
Francesca Romana Buttarelli ◽  
Antonietta Arcella ◽  
Sumihito Nobusawa ◽  
Vittoria Donofrio ◽  
...  
Keyword(s):  
Prognostic Significance ◽  
High Grade ◽  
Histological Grading ◽  
P53 Status ◽  
Idh1 Mutations ◽  
High Grade Gliomas

scholarly journals Correlation of p53 Status with Histopathological Grading of Glial Tumors

2020 ◽  
Vol 12 (22) ◽  
pp. 79-81
Author(s):  
Tabish Hassan ◽  
Sunita Vagha ◽  
Samarth Shukla ◽  
Amoli Belsare
Keyword(s):  
Glial Tumors ◽  
P53 Status ◽  
Histopathological Grading

scholarly journals Correlation between p53 and Mdm2 expression with histopathological parameters in cattle squamous cell carcinomas

2022 ◽  
pp. 10-15
Author(s):  
Waseem Al-Jameel ◽  
S. S. Al-Mahmood ◽  
A. M. Al-Saidya
Keyword(s):  
Squamous Cell ◽  
Histological Grading ◽  
P53 Expression ◽  
Grading Systems ◽  
Mdm2 Expression ◽  
Poorly Differentiated ◽  
Mitotic Figures ◽  
Degree Of Differentiation ◽  
Histopathological Grading

Background and Aim: Squamous cell carcinoma (SCC) is the most common form of carcinoma in cattle. Histopathological grading systems have been utilized over several decades for estimating the malignancy of cattle SCCs. This study aimed to detect p53 and Mdm2 expression in different SCC cases in cattle and correlate their expression with the SCC histopathological grading. Materials and Methods: Cattle SCC cases were collected at the Veterinary Teaching Hospital in Nineveh. The SCC grading system categorized the cases histologically based on their differentiation grade into three groups: Well, moderately, and poorly differentiated. The SCC cases were subsequently verified for p53 and Mdm2 immunoexpression. Results: Fourteen of 16 examined cattle SCC samples tested positive for p53 expression. Moreover, 15 out of the 16 SCC samples tested positive for Mdm2 expression. The increased immunoreactivity of both p53 and Mdm2 was associated with a poor histological grading of the cattle SCC. There is a positive correlation between the nuclear expression of p53 and Mdm2, and the degree of differentiation and the number of mitotic figures in the examined cattle SCC samples. Conclusion: Our results demonstrate an increased p53 and Mdm2 expression in cattle SCC cases characterized by poor histopathological grading, thus suggesting an essential role of these molecules in the development of moderately and poorly differentiated SCC in cattle.

scholarly journals A PERMANENT NEUROSURGICAL CHALLENGE: LOW GRADE GLIOMAS

2016 ◽  
Vol 15 (4) ◽  
pp. 160-167
Author(s):  
Daniel Mihai Teleanu ◽  
◽  
Nicolae-Stefan Bogaciu ◽  
Andreea Marinescu ◽  
Raluca Ioana Teleanu ◽  
...  
Keyword(s):  
Histopathological Examination ◽  
Histological Grade ◽  
Pathological Examination ◽  
Resonance Spectroscopy ◽  
Grading System ◽  
Low Grade ◽  
Low Grade Gliomas ◽  
Tumoral Lesion ◽  
One Year

Introduction. The utilization of Magnetic Resonance Spectroscopy (MRS) brings an important piece of information to an overall MR study, thus aiding the physician in making an accurate assumption regarding the histological grade of a tumor. The purpose of this study is to verify the reliability of MRS in correctly diagnosing both the nature of tumors and their grade. Material and methods. This is an observational study that was conducted from January 2011 to June 2016 on 49 patients confirmed to be low-grade gliomas (LGG) by pathological examination, who were admitted in our Neurosurgery Department in this period. Both retrospective and prospective data were collected. Inclusion criteria comprise unique tumoral lesion at the moment of diagnosis, follow-up for at least one year. Exclusion criteria included: other types of tumors with any location, patient refusal to undergo histopathological examination of the resected tissue, uncompliant and non-collaborating patients. Of all patients, 22 were subjected to an MRS study which suggested the presence of a low-grade glioma subsequently confirmed by the pathological examination. Results. MRS has been shown to provide accurate non-invasive diagnosis of LGG’s, by analyzing the concentration of metabolites inside the lesions which tend to be specific for these tumours: relatively elevated levels of N-acetylaspartate and creatine with reduced levels in the concentration of choline and absent lipids and lactate. It has also been observed that pre-operative MRS assists the physician in selecting the optimal place for biopsy, so that the pathological examination provides conclusive results. Conclusions. With the increased availability of MRI technology, MRS comes as a milestone in the advancement of more accurate and patient-friendly methods of diagnostic for pathologies as LGG’s which constitute a permanent challenge for neurosurgeons. The results of this study underlines the importance of MRS as a method for follow up patients with LGG, but cannot replace the need for obtaining bioptic tissue for pathological examination, especially after the new grading system of WHO, which was published in June 2016. This new grading system takes into account the molecular biology in predicting the natural history of cerebral tumours.

Histological grading and bromodeoxyuridine labeling index of astrocytomas

1991 ◽  
Vol 75 (2) ◽  
pp. 202-205 ◽  
Author(s):  
François Labrousse ◽  
Catherine Daumas-Duport ◽  
Leszek Batorski ◽  
Takao Hoshino
Keyword(s):  
Histological Grade ◽  
Tumor Grade ◽  
Cell Types ◽  
Labeling Index ◽  
Grading System ◽  
Proliferative Potential ◽  
Histological Grading ◽  
Content Type ◽  
Endothelial Proliferation ◽  
Grade 3

✓ The histological grade and the bromodeoxyuridine (BUdR) labeling index of 60 astrocytomas of “ordinary” cell types (fibrillary, protoplasmic, gemistocytic, and anaplastic astrocytomas and glioblastomas) were compared to determine whether the grading system reflects the proliferative potential of the tumors. The tumor grade was based on the presence or absence of four criteria (nuclear abnormalities, mitosis, necrosis, and vascular endothelial proliferation): Grade 1, no criterion, Grade 2, one criterion, Grade 3, two criteria; and Grade 4, three or four criteria. The BUdR labeling index, or percentage of S-phase cells, was calculated in paraffinembedded tumor sections after in situ labeling by intraoperative intravenous infusion of BUdR, 200 mg/sq m. Exponential regression analyses showed a positive correlation between the histological grade and labeling index (r = 0.88, p < 0.001) that was stronger than the correlations between log labeling index and age (r = 0.55, p < 0.001) and between grade and age (r = 0.55, p < 0.001). These results indicate that the histological grading system reflects the proliferative potential of “ordinary” astrocytomas.

scholarly journals Prognostic significance of CEA, Ki67 and p53 in pseudomyxoma peritonei of appendiceal origin

2021 ◽  
Vol 49 (6) ◽  
pp. 030006052110222
Author(s):  
Fengcai Yan ◽  
Feng Shi ◽  
Xinbao Li ◽  
Hong Chang ◽  
Mulan Jin ◽  
...  
Keyword(s):  
Overall Survival ◽  
Pseudomyxoma Peritonei ◽  
Histological Grade ◽  
Prognostic Significance ◽  
Mucinous Carcinoma ◽  
Cox Proportional Hazards ◽  
Nuclear Antigen ◽  
Low Grade ◽  
High Grade ◽  
Biological Behaviour

Objective To determine the levels of carcinoembryonic antigen (CEA), proliferating nuclear antigen Ki67 and p53 in pseudomyxoma peritonei (PMP) of appendiceal origin and to correlate the levels with clinicopathological characteristics and overall survival. Methods This retrospective study collected data on clinicopathological features and immunohistochemical staining of CEA, Ki67 and p53 in patients with PMP of appendiceal origin. Overall survival was evaluated using Kaplan–Meier plots. Median survival time was estimated by Log-rank tests. Potential prognostic factors were evaluated by Cox proportional hazards regression models. Results A total of 141 patients with PMP of appendiceal origin were enrolled in the study with a median age of 54 years. Of these, 93 (66.0%) were diagnosed with low-grade mucinous carcinoma, 43 (30.5%) with high-grade mucinous carcinoma and five (3.5%) with high-grade with signet ring cells. CEA exhibited ubiquitous immunopositivity in most cases and was not associated with overall survival. Ki67 labelling index (LI) and p53 status were related to histological grade and overall survival. The main pathological indicators affecting survival included histological grade, lymph node involvement, angiolymphatic invasion, Ki67 LI and p53. Conclusion Combined analysis of high Ki67 LI and aberrant p53 may provide the basis for evaluating the biological behaviour of PMP and predicting clinical outcome.

scholarly journals Diffuse leptomeningeal glioneuronal tumor with high-grade features masquerading as tubercular meningitis—a case report

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Sameer Peer ◽  
Vivek Murumkar ◽  
Karthik Kulanthaivelu ◽  
Chandrajit Prasad ◽  
Shilpa Rao ◽  
...  
Keyword(s):  
Case Report ◽  
Early Diagnosis ◽  
Histopathological Examination ◽  
Glioneuronal Tumor ◽  
Communicating Hydrocephalus ◽  
High Grade ◽  
Endemic Region ◽  
Tubercular Meningitis ◽  
Leptomeningeal Enhancement ◽  
Diffuse Leptomeningeal Glioneuronal Tumor

Abstract Background Diffuse leptomeningeal glioneuronal tumor (DLGNT) has been recently described in the literature. The complete neuroimaging spectrum and histopathological characteristics of this entity are yet to be elucidated. In an endemic region, diffuse leptomeningeal enhancement on neuroimaging with associated communicating hydrocephalus is usually suggestive of infective meningitis and the patients are started on empirical anti-microbial therapy. However, it is important to consider other differential diagnosis of leptomeningeal enhancement in such cases, particularly if the clinical condition does not improve on anti-microbial therapy. An early diagnosis of a neoplastic etiology may be of particular importance as the treatment regimens vary considerably depending on the underlying disease condition. Case presentation In this case report, we describe a case of DLGNT with high-grade histopathological features which was initially managed as tubercular meningitis based on the initial neuroimaging findings. Due to worsening of the clinical course and subsequent imaging findings at follow-up, a diagnosis of DLGNT was considered and subsequently proven to be DLGNT with features of anaplasia on histopathological examination of leptomeningeal biopsy specimen. Conclusion This case highlights the importance of recognizing certain subtle finding on MRI which may help in an early diagnosis of DLGNT which is crucial for appropriate treatment.

scholarly journals Stereology in Grading and Prognosis of Canine Cutaneous Mast Cell Tumors

2021 ◽  
pp. 030098582098513
Author(s):  
Mafalda Casanova ◽  
Sandra Branco ◽  
Inês Berenguer Veiga ◽  
André Barros ◽  
Pedro Faísca
Keyword(s):  
Mast Cell ◽  
Clinical Outcome ◽  
Prognostic Value ◽  
Histological Grade ◽  
Intraobserver Variability ◽  
Low Grade ◽  
High Grade ◽  
Mast Cell Tumors ◽  
Cutaneous Mast Cell ◽  
Grade Iii

Canine cutaneous mast cell tumors (ccMCTs) are currently graded according to Patnaik and Kiupel grading schemes. The qualitative and semiquantitative parameters applied in these schemes may lead to inter- and intraobserver variability. This study investigates the prognostic value of volume-weighted mean nuclear volume ([Formula: see text]), a stereological estimation that provides information about nuclear size and its variability. [Formula: see text] of 55 ccMCTs was estimated using the “point-sampled intercept” method and compared with histological grade and clinical outcome. The clinical history of dogs treated with surgical excision alone was available for 30 ccMCTs. Statistical differences in [Formula: see text] were found between grade II ([Formula: see text]= 115 ± 29 µm3) and grade III ccMCTs ([Formula: see text]= 197 ± 63 µm3), as well as between low-grade ([Formula: see text]= 113 ± 28 µm3) and high-grade ccMCTs ([Formula: see text]= 184 ± 63 µm3). An optimal cutoff value of [Formula: see text] ≥ 150 µm3 and [Formula: see text] ≥ 140 µm3 was determined for grade III and high-grade ccMCTs, respectively. In terms of prognosis, [Formula: see text] was not able to predict the clinical outcome in 42% of the cases; however, cases with [Formula: see text]<125 µm3 had a favorable outcome. These results indicate that, despite having limited prognostic value when used as a solitary parameter, [Formula: see text] is highly reproducible and is associated with histological grade as well as with benign behavior.

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