scholarly journals Fabrication and Characterization of Chitosan-Polypyrrole/Strontium-Magnesium Substituted Hydroxyapatite Biocomposite with Potential Application in Tissue Engineering Scaffolds

2020 ◽  
Vol 32 (12) ◽  
pp. 3113-3119
Author(s):  
P. Lavanya ◽  
N. Vijayakumari ◽  
R. Sangeetha ◽  
G. Priya
Keyword(s):  
Solvent Casting ◽  
Osteoblast Cell ◽  
Casting Method ◽  
Tissue Engineering Scaffolds ◽  
Osteoblast Cells ◽  
Good Antibacterial Activity ◽  
Osteoblast Cell Line ◽  
Fabrication And Characterization ◽  
Sem Morphology

Biocomposite scaffolds of strontium-magnesium substituted hydroxyapatite (SMHA) with a mixture of chitosan/polypyrrole (CS-PPY) have been prepared by solvent casting method. The synthesized SMHA nanoparticles and biocomposite scaffold were characterized by FTIR, XRD and SEM techniques. The SEM morphology revealed the porous structure of the scaffolds designed for the muticomponents. The biomineralization and cell viability of the biocomposite were assessed via alkaline phosphatase activity and MTT assay on the osteoblast cell line. The study demonstrated improved differentiation and mineralization of osteoblast cells in the designed biocomposite scaffolds. The dye stained fluorescent microscopic photographs apparent the good scattering and permeability of cells onto the scaffolds. However, biocomposite demonstrated good antibacterial activity and the excellent biocompatibility against osteoblast cells offers a possible route for the production of a biocomposite as a viable replacement for regenerative medicine scaffolding substance.

Fabrication and Characterization of MMCs Using Stir Casting Method

2014 ◽  
Vol 4 (3) ◽  
pp. 27-32
Author(s):  
Jeevan Singh Bisht ◽  
◽  
Akshay Dvivedi ◽  
Apurbba Kumar Sharma ◽  
◽  
...  
Keyword(s):  
Stir Casting ◽  
Casting Method ◽  
Fabrication And Characterization

Development and Characterization of Chitosan Membranes as a System for Controlled Release of Piperine

2016 ◽  
Vol 869 ◽  
pp. 864-868
Author(s):  
Imarally Vitor de Souza Ribeiro Nascimento ◽  
Mairly Karolyne da Silva Souza ◽  
Willams Teles Barbosa ◽  
Thiago Bizerra Fideles ◽  
Thais Maria Alves Marinho ◽  
...  
Keyword(s):  
Controlled Release ◽  
Drug Carriers ◽  
Solvent Casting ◽  
X Ray Diffraction ◽  
Casting Method ◽  
Pharmacological Activities ◽  
X Ray ◽  
Natural Alkaloid ◽  
Chitosan Membranes

Among the variety of polymers used as drug carriers, chitosan has received attention in the fields of medicine and pharmacy by being a nontoxic, biocompatible and biodegradable copolymer. Piperine is a natural alkaloid, has various pharmacological activities such as anti-inflammatory, analgesic, antipyretic, hepatoprotective and antitumor when combined with chitosan has better bioavailability and more effectiveness. This work aims to prepare and characterize chitosan / piperine membranes obtained by the solvent casting method. The membranes were characterized by Spectroscopy Fourier Transform Infrared (FTIR), X-ray Diffraction (DRX) and Scanning Electron Microscopy (MEV). From the results obtained it can be concluded that the process described was effective in obtaining the system chitosan/piperine indicating its potential for studies of controlled release of drug.

Preparation and Characterization of Porous Membrane for Drug Release

2007 ◽  
Vol 342-343 ◽  
pp. 485-488
Author(s):  
Soo Young Lee ◽  
Young Ho Cho ◽  
Ju Yong Youn ◽  
Moon Suk Kim ◽  
Bong Lee ◽  
...  
Keyword(s):  
Pore Formation ◽  
Water Solubility ◽  
Porous Membrane ◽  
Solvent Casting ◽  
Casting Method ◽  
Semipermeable Membranes ◽  
Permeable Membrane ◽  
Sem Image ◽  
Water Dissolution

To develop osmotic granule with semi-permeable membranes, we prepared the semipermeable membranes with different pore forming agent by using solvent casting method. The membrane was consisted of cellulose acetate, Eudragit® RS, hydroxypropylcellulose (HPC), and triethylcitrate (TEC) in the presence of PEG200, PEG1, 000, or dibutylsebacate(DBS) as a pore forming agent. The produced membranes were white and elastic and exhibited soft property on touch. The release amount of pore forming agent from membrane with different pore forming agent was measured in water dissolution media and the order was PEG200 > PEG1, 000 > DBS. The formation of pore in membrane was observed by morphological SEM image after dissolution. The pore formation and porosity of membrane depended on water solubility of pore forming agent. We confirmed that pores in porous semi-permeable membrane could be controlled by the pore forming agent.

Fabrication and Characterization of Composite Microspheres Containing Carbonated Hydroxyapatite Nanoparticles

2007 ◽  
Vol 334-335 ◽  
pp. 1221-1224 ◽  
Author(s):  
Wen You Zhou ◽  
Min Wang ◽  
W.L. Cheung
Keyword(s):  
Hydroxyapatite Nanoparticles ◽  
Tissue Engineering Scaffolds ◽  
Sem Images ◽  
Carbonated Hydroxyapatite ◽  
Suitable Material ◽  
Composite Microspheres ◽  
Evaporation Technique ◽  
Nanoparticle Content ◽  
Fabrication And Characterization

Nano-sized carbonated hydroxyapatite (CHAp) particles were firstly synthesized using a nanoemulsion method. TEM analyses revealed that as-synthesized nanoparticles were calcium-deficient and spherical in shape (diameter: 16.8±2.6nm). Biocomposite microspheres comprising CHAp nanoparticles and poly(L-lactide) (PLLA) were fabricated using the single emulsion solvent evaporation technique. SEM images showed that composite microspheres were mainly 5-30 μm in size despite the change of CHAp nanoparticle content. When the CHAp nanoparticle content in composite microspheres was below 10 wt%, all nanoparticles were encapsulated within the microspheres which possessed a nanocomposite structure. DSC results showed that the crystallinity of the PLLA matrix of microspheres increased from 38 to 42% when the CHAp nanoparticle content was increased from 0 to 20 wt%. The biocomposite microspheres should be a suitable material for constructing bone tissue engineering scaffolds.

scholarly journals Bone Formation Ability and Cell Viability Enhancement of MC3T3-E1 Cells by Ferrostatin-1 a Ferroptosis Inhibitor of Cancer Cells

2021 ◽  
Vol 22 (22) ◽  
pp. 12259
Author(s):  
Alireza Valanezhad ◽  
Tetsurou Odatsu ◽  
Shigeaki Abe ◽  
Ikuya Watanabe
Keyword(s):  
Gene Expression ◽  
Cell Death ◽  
Bone Formation ◽  
Cell Viability ◽  
Osteoblast Cell ◽  
Osteoblast Cells ◽  
Alizarin Red ◽  
Activity Test ◽  
Osteoblast Cell Line ◽  
Regulated Necrosis

Recently, ferroptosis has gained scientists’ attention as an iron-related regulated necrosis. However, not many reports have investigated the effect of ferroptosis on bone. Therefore, with the present study, we assessed the effect of ferroptosis inhibition using ferrostatin-1 on the MC3T3-E1 pre-osteoblast cell. Cell images, cell viability, alkaline phosphatase activity test, alizarin red staining, and RUNX2 gene expression using real-time PCR were applied to investigate the effects of ferrostatin and erastin on MC3T3-E1 osteoblast cells. Erastin was used as a well-known ferroptosis inducer reagent. Erastin with different concentrations ranging from 0 to 50 µmol/L was used for inducing cell death. The 25 µmol/L erastin led to controllable partial cell death on osteoblast cells. Ferrostatin-1 with 0 to 40 µmol/L was used for cell doping and cell death inhibition effect. Ferrostatin-1 also displayed a recovery effect on the samples, which had already received the partially artificial cell death by erastin. Cell differentiation, alizarin red staining, and RUNX2 gene expression confirmed the promotion of the bone formation ability effect of ferrostatin-1 on osteoblast cells. The objective of this study was to assess ferrostatin-1’s effect on the MC3T3-E1 osteoblast cell line based on its ferroptosis inhibitory property.

Fabrication of Polyelectrolyte/TiO2 Hybrid Membrane via a Simple Method and Characterization of its Thermal Behavior

2012 ◽  
Vol 602-604 ◽  
pp. 1484-1487 ◽  
Author(s):  
Shu Jun Liang ◽  
Shi Ping Zhu
Keyword(s):  
Thin Films ◽  
Thermal Behavior ◽  
Hybrid Membrane ◽  
Solvent Casting ◽  
Simple Method ◽  
Diallyldimethylammonium Chloride ◽  
Fabrication And Characterization ◽  
Thermal Stability Of ◽  
Scanning Electron

This study deals with the fabrication and characterization of polymer matrix thin films containing high content titanium dioxide (TiO2). Poly(diallyldimethylammonium chloride) (PDADMAC) membrane incorporated with different percentages of TiO2nanoparticles were prepared by solvent casting. The morphologies of the films were characterised by scanning electron microscopy (SEM), and its thermal behavior were characterised by thermogravimetry(TG). The results indicated that dense TiO2/polymer thin films can been fabricated conveniently by solvent casting from aqueous solutions of TiO2/PDADMAC when the concentrations of TiO2were lower than 40 wt%, and the thermal stability of the TiO2/PDADMAC composites were greatly improved by the addition of TiO2moieties.

scholarly journals SIFAT TRANSPARANSI DAN PERMEABILITAS FILM BIONANOKOMPOSIT POLYLACTIC ACID DAN POLYCAPROLACTONE DENGAN PENAMBAHAN NANOCRYSTALLINE CELLULOSE SEBAGAI PENGISI [Transparency and permeability properties of Bionanocomposite Film of Polylactic Acid and Polycaprolactone, and Nanocrystalline Cellulose as a Filler]

2020 ◽  
Vol 25 (2) ◽  
pp. 81
Author(s):  
Eti Indarti ◽  
Arisa Sri Marlita ◽  
Zaidiyah Zaidiyah
Keyword(s):  
Water Vapor ◽  
Polylactic Acid ◽  
Water Vapor Permeability ◽  
Nanocrystalline Cellulose ◽  
Vapor Permeability ◽  
Solvent Casting ◽  
Casting Method ◽  
Empty Fruit Bunches ◽  
Bionanocomposite Films

Production of Polylactic acid (PLA)/Polycaprolactone (PCL) bionanocomposite films with various ratios was done by adding nanocrystalline celullose (NCC) from oil palm empty fruit bunches (OPEFB) as a filler. The aim of the research was to find out the effect of PLA/PCL ratio on film thickness, transparency of bionanocomposite films and water vapor permeability or WVP of the film bionanocomposite with addition of the 3% NCC.  The PLA/PCL ratio are 1.0/0.0; 0.8/0.2; 0.6/0.4; 0.5/0.5; 0.4/0.6; 0.2/0.8; and 0.0/1.0, prepared with solvent casting method. Characterization of PLA/PCL bionanocomposites film performed was thickness, transparency test and water vapor permeability (WVP) test. The thickness of bionanocomposites film produced were around are about 0.036-0.053 mm, results show that the lower PLA/PCL ratio the thicker film obtained. The highest value of film transparency was obtained at a ratio of 1.0 / 0.0 (81.4% at a wavelength of 550 nm), the smaller the PLA / PCL ratio, the lower the value of transparency. The WVP value of PLA/PCL bionanocomposite films gives a lower value than the WVP value of pure PLA film and pure PCL film. The best WVP was obtained at a PLA/PCL ratio of 0.8/0.2 which was 1.49x10-16kg.m/(m2.s.Pa).  

scholarly journals Development of Chitosan-Collagen/Substituted Hydroxyapatite-Polypyrrole Biocomposite with Prospective Application in Bone Tissue Engineering Scaffolds

2020 ◽  
Vol 33 (1) ◽  
pp. 76-82
Author(s):  
P. Lavanya ◽  
N. Vijayakumari ◽  
R. Sangeetha ◽  
G. Priya
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue ◽  
Solvent Casting ◽  
Tissue Engineering Scaffolds ◽  
Osteoblast Cells ◽  
Deterioration Rate ◽  
Physico Chemical ◽  
Copper Manganese ◽  
Prospective Application ◽  
Mechanical Nature

An innovative hybrid based on chitosan-collagen/copper, manganese substituted hydroxyapatitepolypyrrole (CS-COL/CMHA-PPY) was developed in present study to further enhance the surface morphology of regenerative medicine scaffolds by combining the synthesized copper, manganese substituted hydroxyapatite (CMHA) and polypyrrole with the chitosan and collage solution accompanied by solvent casting techniques. The fabricated biocomposite was characterized using FTIR, XRD and SEM techniques. It investigated the effects of CMHA and polypyrrole on the scaffold's physico-chemical characteristics like swelling proportion, degradation, and mechanical nature. The CS-COL/CMHA-PPY biocomposite demonstrated lower deterioration rate and higher mechanical properties according to the chitosan-collagen and CS-COL/CMHA biocomposite. MTT assay conducted a tentative evaluation of the tissue engineering and cytotoxicity of the chitosan-biocomposite scaffold utilizing osteoblast cells. Tests demonstrated no toxicity, so osteoblast cells were bound to the biocomposite pore surfaces so propagated. Such results indicated that the scaffold established has the preconditions which can be used as a scaffold for the reconstruction of bone tissue.

Sign in / Sign up

Export Citation Format

Share Document