scholarly journals Chromatographic Estimation of Water Soluble Vitamins in Wild Edible Plants

2021 ◽  
Vol 33 (11) ◽  
pp. 2845-2850
Author(s):  
Tapan Seal ◽  
Kausik Chaudhuri ◽  
Basundhara Pillai
Keyword(s):  
Limit Of Detection ◽  
Acetic Acid Solution ◽  
Reversed Phase ◽  
Water Soluble ◽  
Wild Edible Plants ◽  
Simultaneous Estimation ◽  
Eastern Region ◽  
Chromatographic Technique ◽  
Edible Plants ◽  
Vitamin B9

A reversed-phase high performance liquid chromatographic technique has been developed for the simultaneous quantitation of water soluble vitamins in 10 potent wild edible plants consumed by the tribal people of North-eastern region in India. The chromatographic separations of vitamins were assessed on Acclaim C18 column using a mobile phase of acetonitrile and aqueous trifluoro acetic acid solution with gradient elution. The experimental results exhibited that for different plants, the vitamin C content ranged between 0.15 ± 0.003 to 8.10 ± 0.03 mg/100g dry plant material (DPM). The vitamin B2 content was determined high in C. album (2.64 ± 0.03 mg/100g DPM) and significant amount of vitamin B9 (1.44 ± 0.03 mg/100g) was detected in E. acuminata. The results showed that these plants are rich sources of vitamins, which can contribute immensely to nutrition and food security. The high percentage of recovery and low limit of detection confirm the suitability of the method for simultaneous estimation of vitamins in these 10 wild edible plants.

Microscopic and Phytochemical analysis of Desmodium velutinum (Willd) DC and Desmodium gangeticum (L.) DC. Panchanga powder

2021 ◽  
pp. 2950-2956
Author(s):  
Jigyasa Pathak ◽  
Aswathi M. P. ◽  
B. R. Patel ◽  
Harisha C. R. ◽  
Shukla Vinay J.
Keyword(s):  
Chemical Analysis ◽  
Analytical Study ◽  
Water Soluble ◽  
Wild Edible Plants ◽  
Phytochemical Analysis ◽  
Edible Plants ◽  
Therapeutic Purpose ◽  
Whole Plant ◽  
Physico Chemical ◽  
Collection Practices

Background: Desmodium velutinum (Fabaceae) is one of the wild edible plants used traditionally to treat diarrhoea, haematuria, infertility and also externally used to treat scorpion bites. Though various parts of the plant have been used for therapeutic purpose, panchanga of the plant has not been studied for its Pharmacognostical and phytochemical characteristics in detail, following scientific parameters. Hence in the present study Desmodium velutinum whole plant has been studied for its pharmacognostical and phytochemical characters and compared with commonly used species Desmodium gangeticum. Methods: Whole plant of Desmodim velutinum was collected from Paikmal, Odisha and Desmodium gangeticum was collected from Junagarh, Gujarat in the month of November 2019 following good collection practices and shade dried. Pharmacognostical study and analytical study were performed following standard pharmacopoeial procedures. Results: Whole plant (Panchanga) of former is sweetish and latter is bitter. Powder microscopy of Desmodium gangeticum showed presence of rhomboidal crystals and lignified annular and spiral vessels. Desmodium velutinum showed presence of prismatic crystals and lignified pitted vessels. Physico-chemical analysis showed higher loss on drying in Desmodium gangeticum, and higher water soluble extractive values of Desmodium velutinum. Phytochemical estimation showed the presence of Proteins, tannins, and carbohydrates in both samples. HPTLC study showed similar Rf value 0.82 and 0.94 at 254 and 0.03 and 0.41 at 366nm. Conclusion: Basing upon the analytical and microscopic similarities Desmodium velutinum may be considered as substitute of Desmodium gangeticum

scholarly journals RP-HPLC analysis for the simultaneous estimation of rabeprazole sodium and aceclofenac in a combined dosage form

2012 ◽  
Vol 1 (12) ◽  
pp. 410-413 ◽  
Author(s):  
Sukhbir Lal Khokra ◽  
Balram Choudhary ◽  
Heena Mehta
Keyword(s):  
High Performance ◽  
Dosage Form ◽  
Limit Of Detection ◽  
Pharmaceutical Journal ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Simultaneous Estimation ◽  
Rp Hplc ◽  
Rabeprazole Sodium

A rapid, simple and highly sensitive reversed phase high performance liquid chromatographic (RP-HPLC) method has been developed for the quantitative determination of Rabeprazole sodium and Aceclofenac in a combined dosage form. Rabeprazole sodium and Aceclofenac were chromatographed using C-18 column as stationary phase and methanol: acetonitrile: water (60 : 10 : 30 v/v/v) as the mobile phase at a flow rate of 1.0 ml/min at ambient temperature and detected at 280 nm. The retention time (RT) of Rabeprazole sodium and Aceclofenac were found to be 5.611 min and 2.102 minute, respectively. The linearities of Rabeprazole sodium and Aceclofenac were in the range of 1-10 µg/ml and 3-15 µg/ml, respectively. The limit of detection was found to be 0.091 µg/ml for Rabeprazole sodium and 0.043 µg/ml for Aceclofenac. The proposed method was applied for the determination of Rabeprazole sodium and Aceclofenac in a combined dosage form and result was found satisfactory.DOI: http://dx.doi.org/10.3329/icpj.v1i12.12450 International Current Pharmaceutical Journal 2012, 1(12): 410-413

scholarly journals A Simplified, Specific HPLC Method of Assaying Thiamine and Riboflavin in Mushrooms

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Mohammad F. Hossain ◽  
Mamoon Rashid ◽  
Rajjit Sidhu ◽  
Randy Mullins ◽  
Susan L. Mayhew
Keyword(s):  
High Performance ◽  
Limit Of Detection ◽  
Extraction Process ◽  
Reversed Phase ◽  
Hplc Method ◽  
Water Soluble ◽  
Qualitative And Quantitative Analysis ◽  
Food Industries ◽  
Qualitative And Quantitative ◽  
Rp Hplc

Mushrooms have been used as part of the average diet and as a nutraceutical for thousands of years due to their immense health benefits. The purpose of this study was to develop a simple, fast, accurate, specific, reproducible, and robust chromatographic method to identify and quantify two water-soluble vitamins: thiamine (B1) and riboflavin (B2) in mushrooms. The method employed for qualitative and quantitative analysis of these vitamins was Reversed Phase-High Performance Liquid Chromatography (RP-HPLC) equipped with Ultraviolet–Visible (UV-Vis) Detector. The extraction process involved acid hydrolysis followed by enzymatic dephosphorylation with takadiastase enzyme. Chromatographic separation was achieved with a Shimadzu prominence HPLC system using isocratic elution mode on a Waters Xterra® MS C-18 column (4.6mm × 150mm, 5 μm) integrated with a XBridge® BEH C-18 Guard column (2.1mm × 5 mm, 5 μm). The mobile phase of this study consisted of buffer and methanol in the ratio of 80:20, where the buffer contained sodium-1-hexanesulfonate, glacial acetic acid, methanol, and pH adjusted to 3.0 with diethylamine. Vitamins were detected simultaneously at their lambda max wavelengths B1: 245nm and B2: 268nm using dual-wavelength UV detection technique to get their highest response. The proposed method was found to be specific, linear R>1.0, accurate, precise (% recovery ± SD; B1:104.45±4.5 and B2: 104.88±2.04), sensitive, (limit of detection for B1 and B2 was 0.043 and 0.029 μg/mL, respectively), and robust for mushrooms analysis. No coeluting peaks were observed at the retention time of the vitamins and all the peaks were spectrally homogenous. The standard and sample solutions were found to remain stable at cold temperature for 72 hours. In summary, our data suggest that the proposed method could be used in food industries to monitor the product quality during routine quality control purposes.

EFFECT OF BOILING AND MICROWAVE COOKING ON NUTRITIONAL, ANTI-NUTRITIONAL AND TOXICITY OF WILD EDIBLE PLANTS OF NORTH-EASTERN REGION IN INDIA

2020 ◽  
pp. 61-70
Author(s):  
TAPAN SEAL
Keyword(s):  
Wild Edible Plants ◽  
Wild Plants ◽  
Local Market ◽  
Eastern Region ◽  
Edible Plants ◽  
Microwave Cooking ◽  
Cytotoxicity Studies ◽  
North Eastern ◽  
Wild Edibles

Objective: The target of this appraisal was to explore the impacts of various cooking techniques, for example, boiling and microwave cooking on nutritional, antinutritional, minerals content and in vitro toxicity of ten wild consumable plants viz. Zanthoxylum acanthopodium, Viburnum foetidum, Houttuynia cordata, Sonchus arvensis, Oenanthe linearis, Perilla ocymoides, Clerodendrum colebrookeanum, Solanum gilo, Solanum kurzii, Potentilla lineata, widely consumed by the common individuals of North-Eastern area in India. Methods: The proximate parameters like ash, moisture, protein, fat, fibre, carbohydrate, energy content, minerals viz. sodium, calcium, potassium, iron, magnesium, manganese, copper, zinc and antinutritional parameters like oxalate, phytate, tannin, saponin, cyanogenic glycoside content were evaluated in the selected wild edible plants using standard food analysis techniques. In vitro haemolytic toxicity of aqueous extracts (100, 300 and 500µg/cc) of ten palatable wild plants was done with the blood samples were gathered from healthy rat, mixed with Ethylenediaminetetraacetic acid(EDTA) and centrifuged at 5,000 Revolutions Per Minute (rpm) for five minutes. The 10 % erythrocyte suspension was set up in sterile Phosphate buffer saline (PBS, pH 7.4) for haemolytic examination. The genotoxic potential of the concentrates were assessed by a single-cell gel electrophoresis comet test. Cytotoxicity studies were evaluated with fresh goat livers procured from the local market were perfused in PBS (pH 7.4) with collagenase and the liver was then minced in minute pieces and cells were isolated utilizing cell strainer. Results: Both cooking medications diminished the congregations of ash, fat, minerals, antinutritional parts and the destructive nature of the consumable plants while the carbohydrate and fiber substance were expanded. The protein focuses in the wild edibles were expanded fundamentally (P<0.05) in the range from 1.26 to 10.45% on microwave cooking while the indistinguishable were exhausted in the range out of 2.20 to11.55% on boiling treatment. The microwave cooking demonstrated lesser misfortunes in minerals in the consumable plants than those cooked by frothing. The microwave cooking also caused the colossal rot (P<0.05) of antinutritional parameters and damaging tendency to a more significant degree than the sputtering medications of the wild edibles. Conclusion: Therefore, the outcomes uncovered that microwaving of appealing plants could be prescribed to expand the supporting quality and to diminish the fat, threatening to dietary structure and lethality. The toxicity assessment of the consumable plants at cell and genomic level showed that these are harmless to consume.

scholarly journals Ultra Sensitive UPLC Method Development and Validation for the Simultaneous Estimation of Tamsulosin Hydrochloride and Tolterodine Tartrate in Bulk and Pharmaceutical Dosage Form

2018 ◽  
pp. 50-59
Author(s):  
Mahmoud M. Sebaiy ◽  
Sobhy M. El-Adl ◽  
Mohamed Baraka ◽  
Mostafa S. Mohram ◽  
Fatma Ibrahim
Keyword(s):  
Dosage Form ◽  
Method Development ◽  
Limit Of Detection ◽  
Pharmaceutical Preparation ◽  
Potassium Phosphate ◽  
Reversed Phase ◽  
Simultaneous Estimation ◽  
Limit Of Quantification ◽  
Pharmaceutical Dosage Form ◽  
Significant Difference

A rapid, sensitive and accurate ultra-performance reversed phase liquid chromatographic method was developed for the simultaneous determination of tamsulosin and tolterodine in pure form and pharmaceutical preparation. The developed UPLC method is superior to conventional HPLC with respect to speed, resolution, solvent consumption and cost. The separation was carried out on RP C18 nucleosil (1.7 µm, 5 cm x 2 mm) using an isocratic mode in eluting Tamsulosin and Tolterodine at 1.54 min and 2.43 min respectively with a mobile phase composed of acetonitrile and 0.025N potassium phosphate buffer pH 3.50 (60%:40%), respectively. Chromatographic run time was 5 min with a flow rate 0.5 ml/min and UV detection at 220 nm. The linearity for tamsulosin and tolterodine were in the range of 2-20 µg/mL for both drugs, showed excellent recoveries for bulk and tablet dosage form with a very low LOD of 4.29 and 0.59 ng/mL for tamsulosin and tolterodine, respectively. The method has been validated for linearity, accuracy, precision, specificity, and limit of detection, limit of Quantification, robustness, and ruggedness. The method which was developed was validated as per the ICH guidelines. Finally, the method was compared statistically with reference methods indicating that there is no significant difference between them in respect of precision and accuracy.

Validated RP-HPLC method for the estimation of nebivolol hydrochloride and valsartan in combined tablet dosage form

2021 ◽  
pp. 168-172
Author(s):  
R. Anantha Kumar ◽  
G. Raveendra Babu ◽  
Sowjanya M. ◽  
Ramayyappa M.
Keyword(s):  
Dosage Form ◽  
Limit Of Detection ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Chromatographic Technique ◽  
Limit Of Quantification ◽  
Accuracy And Precision ◽  
High Degree ◽  
Tablet Dosage ◽  
Liquid Chromatographic

The intention of this work is to develop a rapid, precise, accurate and sensitive reverse phase liquid chromatographic technique for the simultaneous estimation of Nebivolol and Valsartan in tablet dosage form. The chromatographic method was standardized using Inertsil ODS column (250×4.6mm, 5μm particle size) with UV detection at 278nm and flow rate of 1ml/min. The mobile phase comprises of ACN: Buffer (pH adjusted to 3.5 with dilute Ortho Phosphoric acid) in the fraction of 60:40 v/v. The linearity of proposed method was investigated in the range of 12.5-62.5μg/ml (R²=0.999) for Nebivolol and 200-1000μg/ml(R²=0.999) for Valsartan duly. The limit of detection (LOD) was found to be 0.05μg/ml and 0.81μg/ml for Nebivolol and Valsartan duly. The limit of quantification (LOQ) was found to be 0.15μg/ml and 2.44μg/ml for Nebivolol and Valsartan respectively. The retention time of Nebivolol and Valsartan were ground in to be 4.400min and 2.568min respectively. The method was authentically suggested and % RSD was ground in to be less than 2 indicating high degree of accuracy and precision. Hence proposed method can be successfully graded for the simultaneous estimation of Nebivolol and Valsartan in marketed formulation.

scholarly journals CLEANING VALIDATION OF A SIMPLE AND RAPID REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR THE SIMULTANEOUS ESTIMATION OF ASPIRIN AND ROSUVASTATIN

2019 ◽  
pp. 261-265
Author(s):  
CELINA NAZARETH ◽  
GISELLE FIZARDO ◽  
CHARMAINE VAZ
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Reversed Phase ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Dihydrogen Phosphate ◽  
Cleaning Validation ◽  
Rp Hplc

Objective: This study describes a new, simple, precise, accurate, and reproducible reversed-phase high-performance liquid chromatography (RP-HPLC) cleaning validation method for simultaneous estimation of rosuvastatin and aspirin. Methods: The proposed RP-HPLC method was carried out on AGILENT-ZORBAX RP-Inertsil column (250 mm × 4.6 mm, 5 μm) in an isocratic mode utilizing potassium dihydrogen phosphate buffer (pH 2.5 with OPA):acetonitrile (50:50,v/v) as mobile phase, at a flow rate of 1.5 ml/min. Detection was carried out at 243 nm using UV detector. Results: The method was found specific as there was no swab interference. The Beer–Lambert’s law was obeyed in the concentration range of 0.5–20 μg/ml for both rosuvastatin and aspirin. The mean percentage recoveries at 100% level were 89.4% for rosuvastatin and 82.1% for aspirin. The limit of detection and limit of quantification for rosuvastatin and aspirin were 0.03 μg/ml and 0.1 μg/ml, respectively. The method was found to be robust and precise with percentage RSD <2.0%. Conclusion: A simple, novel, and economical RP-HPLC method for cleaning validation has been developed for the simultaneous estimation of rosuvastatin and aspirin. The method was validated as per ICH guidelines for specificity, linearity, accuracy, precision, and robustness. The developed method can be used as a sensitive analytical tool for ensuring the effectiveness of the cleaning procedure adopted.

scholarly journals Determination of Lincomycin Residue in Salmon Tissues by Ion-Pair Reversed-Phase Liquid Chromatography with Electrochemical Detection

1996 ◽  
Vol 79 (4) ◽  
pp. 839-843 ◽  
Author(s):  
Wenhong Luo ◽  
Eugene B Hansen ◽  
Catharina Y W Ang ◽  
Harold C Thompson
Keyword(s):  
Liquid Chromatography ◽  
Electrochemical Detection ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Reversed Phase ◽  
Ion Pair ◽  
Water Soluble ◽  
Reversed Phase Liquid Chromatography ◽  
Limit Of Quantitation ◽  
Phase Liquid

Abstract A method is described for detecting and quantitating lincomycin residue in salmon muscle and skin tissues by ion-pair reversed-phase liquid chromatography (LC) with electrochemical detection at +0.9 V. Lincomycin was extracted from tissues by homogenizing with 0.01 M KH2PO4 buffer (pH 4.5) and centrifuging the mixture. Water-soluble proteins were precipitated by adding sodium tungstate and sulfuric acid and removed by cent r if u gat ion. The buffer extract was then passed through a C18 solid-phase extraction cartridge. Lincomycin was eluted with 50% acetonitrile in water, and the eluate containing lincomycin was extracted with ethyl acetate. After the solvent had evaporated, the residue was redissolved in mobile phase and analyzed by LC. The method had a limit of detection of 7 ng/g lincomycin for salmon muscle and 12 ng/g for salmon skin. The limit of quantitation was 17 ng/g for salmon muscle and 24 ng/g for salmon skin. Average recoveries of lincomycin spiked at 50,100, and 200 ng/g were ≥85% for salmon muscle and ≥80% for salmon skin.

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