Microscopic and Phytochemical analysis of Desmodium velutinum (Willd) DC and Desmodium gangeticum (L.) DC. Panchanga powder

Author(s):  
Jigyasa Pathak ◽  
Aswathi M. P. ◽  
B. R. Patel ◽  
Harisha C. R. ◽  
Shukla Vinay J.

Background: Desmodium velutinum (Fabaceae) is one of the wild edible plants used traditionally to treat diarrhoea, haematuria, infertility and also externally used to treat scorpion bites. Though various parts of the plant have been used for therapeutic purpose, panchanga of the plant has not been studied for its Pharmacognostical and phytochemical characteristics in detail, following scientific parameters. Hence in the present study Desmodium velutinum whole plant has been studied for its pharmacognostical and phytochemical characters and compared with commonly used species Desmodium gangeticum. Methods: Whole plant of Desmodim velutinum was collected from Paikmal, Odisha and Desmodium gangeticum was collected from Junagarh, Gujarat in the month of November 2019 following good collection practices and shade dried. Pharmacognostical study and analytical study were performed following standard pharmacopoeial procedures. Results: Whole plant (Panchanga) of former is sweetish and latter is bitter. Powder microscopy of Desmodium gangeticum showed presence of rhomboidal crystals and lignified annular and spiral vessels. Desmodium velutinum showed presence of prismatic crystals and lignified pitted vessels. Physico-chemical analysis showed higher loss on drying in Desmodium gangeticum, and higher water soluble extractive values of Desmodium velutinum. Phytochemical estimation showed the presence of Proteins, tannins, and carbohydrates in both samples. HPTLC study showed similar Rf value 0.82 and 0.94 at 254 and 0.03 and 0.41 at 366nm. Conclusion: Basing upon the analytical and microscopic similarities Desmodium velutinum may be considered as substitute of Desmodium gangeticum

2021 ◽  
Vol 8 (8) ◽  
pp. 440-449
Author(s):  
Fathima Suman P ◽  
Minhaj . ◽  
I Arthi

This study deals with the Pharmacognostical physicochemical and phytochemical evaluation of the whole plant of Piper longum L. Pharmacognostical studies include macroscopic, microscopic and physicochemical evaluation. Physicochemical constants showed the values of total ash (10.8%), acid insoluble ash (1.3%), water soluble ash (3.2%), sulphated ash (12%), nitrated ash (1.85%), hot extraction (11.2%), water soluble extractive (4.8%), ethanol soluble extractive (12.4%), ether soluble extractive (5.6%), loss on drying (29%), swelling index (0.33) and mucilage content (0.8%) w/w. The preliminary phytochemical screening of the alcoholic extract showed presents of alkaloids, phenolic compounds, tannins, mucilage, starch, steroid and flavonoids. This study helps to development of different standard parameters of the plant which help the plant identification and authentication. Keywords: Piper longum L., Whole plant, Pharmacognostic, Phytochemical, Extract.


Author(s):  
Abhishek Abhishek ◽  
Ashok Kumar BN ◽  
Sujatha K. ◽  
Sunil Kumar

Introduction: Mrityunjaya Rasa is a herbo–mineral formulation mentioned under Jwara Chikitsa in Rasendra Sara Sangraha along with different Anupanas. It is having ingredients like Shudha Hingula, Shudha Gandhaka, Shudha Vatsanabha, Shudha Tankana, Pippali and Maricha which show significant result on various types of fever. Aim: To prepare Mrityunjaya Rasa as per guidelines given in classical text book Rasendra Sara Sangraha and to validate physico-chemical analysis of the sample. Methods: The preparation of Mrityunjaya Rasa was carried out at practical hall, Department of Rasashastra and Bhaishajya Kalpana, Shri Dharmasthala Manjunatheshwara College of Ayurveda, Kuthpady, Udupi, Karnataka. It was subjected to analysis on parameters like organoleptic characters, loss on drying, total ash, acid insoluble ash, water soluble ash, pH, alcohol soluble extractive value, and water soluble extractive value. Results: The results of Analytical study showed specific characteristics which are in accordance with the properties of Mrityunjaya Rasa. Conclusion: The study showed significant results, which proves quality of the drugs and efficacy.


Author(s):  
Sorab Gaind ◽  
Pradeep Agnihotri

Analytical study of any drug is essential to standardize it. Analytical study is carried out to check drug quality. For this purpose some analytical tests are performed and their results are compared with standard parameters. The drug fulfilling these criteria can be taken as standard drug and can be used for therapeutic purpose. Percentage of total mercury in Shadguna Kajjali and Shadguna balijarita rasasindur was 40.03%, and 84.17%. Total Sulphur in Shadguna Kajjali and Shadguna balijarita rasasindur was 43.07% and 11.16%. Percentage of free sulfur in Shadguna Kajjali and Shadguna balijarita rasasindur was 22.11%. XRD of Shadguna balijarita Rasasindur were identified as Cinnabar (Hgs) with Hexagonal Crystal Structure having primitive lattice. In this paper Physico-Chemical Analysis of Shadguna Kajjali and Shadguna balijarita rasasindura is done.


2014 ◽  
Vol 1 (2) ◽  
pp. 105-108
Author(s):  
Sharmila S ◽  
Kalaichelvi K ◽  
Jayanthi G

Medicinal plants are an integral component of alternative medical care. For millennia, Indian people traditionally played an important role in the management of biological resources and were custodians of related knowledge that they acquired through trial and error over centuries. India has a rich wealth ofmedicinal plants and the potential to accept the challenge to meet the global demand for them. In recent time, focus on plant research has increased all over the world and a large body evidence has collected to show immense potential of medicinal plants used in various traditional system. Plumbago zeylanica L.(Plumbaginaceae) is a tropical plant and the source for the well known ayurvedic drug ‘chitrakah’. Different parts of this plant are widely used for variety of medicinal treatments. Especially used for appetite, indigestion, piles, worms, liver diseases and cancer treatments. The present study was therefore carried outto provide pharmacognostic details of root of Plumbago zeylanica. The physico-chemical analysis of ash content indicated the presence of inorganic matter and siliceous matter in the drug. The phytochemical analysis indicated the presence of alkaloids, phenols, flavonoids, tannins and terpenoids. Plumbagin is anaturally occurring yellow pigment, produced by member of plumbaginaceae that can be obtained from roots


2020 ◽  
Vol 5 (05) ◽  
pp. 214-219
Author(s):  
Arya Elias ◽  
Pramod C. Baragi ◽  
Kashinath Hadimur ◽  
K. A. Patil ◽  
Khazi Rahimbi

Background: Shilajatu or Adrija is one of the Maharasa, which is considered as a wonderful medicine in Ayurveda. It is named as it comes out of the stones heated by the sun in summer in the form of thick blackish exudation having many shades. Since it contain stone, mud, wood, sand and various physical and metallic impurities, Shodhana (Purification) of Shilajatu is a mandatory procedure. It has been used as a prime ingredient in many formulations mainly for Prameha, Sotha, Pandu Roga, Kshaya, Swasa, Pliha Vrudhi, Jwara, Agnimandya, Apasmara, etc. Objectives: Shodhana of Ashudha Shilajatu and Physico chemical analysis of Shodhita Shilajatu. Materials and Methods: Bhringaraja Swarasa for Shodhana of Shilajatu. Results:It took 8 days for completion of Shilajatu Shodhana. XRD Analysis report indicates that the sample Shilajatu was Amorphous material. Conclusion: Total yield of Shodhita Shilajatu was 99.6%. The Sample of Shilajatu was found to be Amorphous material in XRD Analysis hence crystal structure was not identified.


Author(s):  
Akhilraj A.R ◽  
Rukmini S ◽  
Amalraj A.R

Background: Citraka or Sveta Citraka (Plumbago zeylanica L.) is an important medicinal plant mentioned in Ayurvedic classics, belongs to the family Plumbaginaceae. The root of the plant exhibits medicinal properties. It has a broad range of pharmacological activities, including anti-inflammatory, antibacterial, antifungal, anticancer, antidiabetic, antioxidant, hepatoprotective, wound healing and cytotoxic. Objective: The aim of the study was to evaluate phyto-pharmacognostical standardization with HPTLC fingerprinting of root of P. zeylanica, one of the most accepted and beneficial medicinal plant in Ayurveda. Materials and Methods: P. zeylanica was collected from Konni, Kerala, India during the month of May 2018 and its root was studied for macroscopic, microscopic, physico-chemical, preliminary phytochemical screening and HPTLC fingerprinting following the standard protocol recommended by The Ayurvedic Pharmacopoeia of India and Quality Standards of Indian Medicinal Plants. Results: The root is long, stout, cylindrical and reddish to dark brown in colour. The transverse section of root showed 5-7 rows of small cubical or rectangular celled cork with light yellow walls, polygonal or slightly tangentially elongated cortex cells containing starch grains and coloured contents, phloem containing small groups of fibres, radially arranged xylem vessels and narrow, 1-4 seriate medullary rays. Physico-chemical analysis showed water soluble extractive value as 26.11±0.01 w/w and alcohol soluble extractive value as 15.85±0.00 w/w. Preliminary phytochemical analysis of root decoction showed the presence of alkaloids, carbohydrates, tannins, saponins, resin and quinones. Conclusion: The information obtained from this research work may be useful to establish the botanical as well as analytical standards for the root of P. zeylanica.


2021 ◽  
Vol 12 (3) ◽  
pp. 538-543
Author(s):  
Ambika S ◽  
Gazala Hussain

Introduction: Lepa is a bahirparimarjana chikitsa that helps in bringing samyata in sthanika dosha and dhatu. Many lepa are mentioned in Ayurveda pharmaceutics for the treatment of mukhadooshika. Among it, one is a combination of Lodhra, Kustumburu, Vacha, Vatapallava, and Narikelashuktya. Mukhadooshika is a type of kshudraroga, featuring the formation of pidaka on face. The symptoms of mukhadooshika resembles with acne vulgaris. Aim: An attempt is made to analyze the physico-chemical parameters of the mukhadooshikahara lepa churna. Materials and methods: The pharmaceutical preparation is easy and simple with easily available ingredients. The organoleptic characters like appearance, odour, taste and physico-chemical parameters like pH, total ash. Water Soluble ash, Alcohol soluble extractives, Water soluble extractives, loss on drying and HPTLC were carried out. Observations and results: The obtained results were discussed in the present paper. Discussion and conclusion: Mukhadooshikahara lepa churna is a simple preparation and can be prepared by easily available drugs. HPTLC fingerprinting at different wavelengths was carried out. At 254 nm, 366nm and 620nm 11, 8 and 10 peaks were found with different retention factor starting from 0.02 to 0.80, 0.03 to 0.096 and 0.07 to 0.80respectively. The analytical study findings can be taken as a preliminary standard for mukhadooshikahara lepa churna.


2021 ◽  
Vol 6 (4) ◽  
pp. 217-221
Author(s):  
JM Dahanayake ◽  
◽  
PK Perera ◽  
P Galappatty ◽  
P Fernando ◽  
...  

Tinospora cordifolia (wild) Hook.f. (Thomas) is an important medicinal plant distributed throughout in Sri Lanka and commonly known as Rasakinda in Sinhala and Giloy in English. It is widely used in Sri Lankan traditional medical system and Ayurveda for the treatment of diabetes mellitus, fever, arthritis, skin diseases and for Rasayana (rejuvenating) therapies due to its anti-inflammatory, hypoglyceamic, immunomodulatory, antioxident, anti-allergy, antipyretic, antiarthritic and various other medicinal properties. Imported T. cordifolia stems and Sri Lankan grown T. cordifolia stems can be found in Sri Lankan herbal market. Moreover, different varieties of Tinospora species known as Tikthakinda, Bukinda and Gatakinda are available in the market under the name of Rasakinda which leads to adulteration. Hence, in this research an attempt was made to develop standards for genuine T. cordifolia grown in Sri Lanka. Stems of T. cordifolia evaluated for macroscopical, microscopical, physico-chemical, phytochemical constituents, TLC and HPTLC fingerprint patterns. Microscopical examinations of T. cordifolia stem exhibited the wheel shaped appearance at the transverse cut surface, which is a main characteristic feature of the family Menespermaceae. Stem also showed the mucilage cells and abundant starch granules. Percentages of total ash, water soluble ash and acid insoluble ash, were 9.1%+0.1, 2.31%+0.1, <0.1% respectively. Among the percentages of extractable matter of T. cordifolia, highest amount was shown in hot water extract (16.2 %+0.3). Heavy metals (Hg, As, Cd, Pb) were within the limits given in WHO guidelines. Phytochemical screening revealed the presence of phenols, saponins, tannins, steroids, flavonoids, terpenoids and cardiac glycosides. TLC fingerprint of T. cordifolia was developed using butanol: ethyl acetate: acetic acid: water in a ratio of 5: 8: 6:2 v/v and compared with one of its marker compound, Berberine. The HPTLC fingerprint patt ern of T. cordifolia showed a spot bearing the same Rf value corresponds to Berberine, at wavelength 254 nm. In conclusion, the results obtained from this study can be used as a standard reference for Sri Lankan grown T. cordifolia stems.


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